The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accur...The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors.In this study,the expression profiles of ten candidate genes,ACT1,ACT2,GAPDH,eIF1,eIF2,α-TUB,β-TUB,TBP,RNA Pol II and RP II,were calculated for a suitable reference gene selection in Paeonia ostii T.Hong et J.X.Zhang leaves under various drought stress conditions.Data were processed by the four regularly used evaluation software.A comprehensive analysis revealed that RNA Pol II was the most stable gene and eIF2 was the least stable one.In addition,the geNorm program provided the optimal choice of two reference gene combination,RNA Pol II andβ-TUB,for qRT-PCR normalization in P.ostii subjected to different drought stress levels.Our research provided convenience for gene expression analysis in P.ostii under drought stress and promoted research of effective methods to alleviate P.ostii drought stress in the future.展开更多
[Objectives] This study was conducted to investigate the effects of different treatments on rooting and germination of Paeonia rockii T. Hong et J.J.Li seeds. [Methods] Different stratification time and temperatures a...[Objectives] This study was conducted to investigate the effects of different treatments on rooting and germination of Paeonia rockii T. Hong et J.J.Li seeds. [Methods] Different stratification time and temperatures and different GA_3 concentrations and treatment time were set to screen the optimum treatment method for promoting seed rooting and germination. [Results] The rooting rate was the highest when the seeds were subjected to 30 d of stratification treatment in an incubator at 25℃, and treated with 300 mg/L GA_3 for 24 h. Soaking the seeds with 300 mg/L GA_3 for 24 h combined with 30 d of stratification treatment was most beneficial to germination, and the germination rate was over 90%. [Conclusions] This study provides technical guidance and basic information for the rooting and germination of P. rockii seeds.展开更多
在黑龙江省东南部地区的海林市进行紫斑牡丹[Paeonia rockii(S.G.Haw et L.A.Lauener)T.Hong et J.J.Li]苗木的栽培试验,连续3年对紫斑牡丹的生长发育表现、物候期进行了观测,采用不同防寒措施进行对比试验。结果表明,紫斑牡丹苗木部分...在黑龙江省东南部地区的海林市进行紫斑牡丹[Paeonia rockii(S.G.Haw et L.A.Lauener)T.Hong et J.J.Li]苗木的栽培试验,连续3年对紫斑牡丹的生长发育表现、物候期进行了观测,采用不同防寒措施进行对比试验。结果表明,紫斑牡丹苗木部分植株地下根系不做防寒处理能正常越冬,但地上枝条冻害严重,保存率逐年降低,长势弱,每年生长量不明显;物候期与原产地相比均较晚;采用不同防寒措施对其萌芽期和始花期影响较大,对其成活率也有一定影响。展开更多
开展了凤丹牡丹(Paeonia ostii T.Hong et J.X.Zhang)种子的特性观测并进行了种子分级试验;采用不同浸种温度、不同浸种时间、沙藏层积、不同浓度激素处理凤丹牡丹种子,筛选出能够促进种子萌发的最佳处理方式。结果表明,利用凤丹种子的...开展了凤丹牡丹(Paeonia ostii T.Hong et J.X.Zhang)种子的特性观测并进行了种子分级试验;采用不同浸种温度、不同浸种时间、沙藏层积、不同浓度激素处理凤丹牡丹种子,筛选出能够促进种子萌发的最佳处理方式。结果表明,利用凤丹种子的比重不同,采取水浸法进行种子分级,能有效区分种子优劣,且Ⅱ级种子的发芽率最高,达到99%;赤霉素(GA3)处理能大幅提高播种出苗率,GA3促进种子萌发的的最佳浓度为150 mg/L,种子萌发率达到99%。种子的品质与质量相关性不大,与吸水率呈反比;凤丹牡丹种子萌发需要一定的生理后熟期。展开更多
琼岛杨(Populus qiongdaoensis T.Hong et P.Luo)为杨柳科,杨属,落叶大乔木。该珍贵树种在海南岛霸王岭高山地区被发现,它的发现打破了杨树在我国南缘分布的纬度界限,是海南林业的一新发现。琼岛杨迁地培育新技术研究课题组,经过深入调...琼岛杨(Populus qiongdaoensis T.Hong et P.Luo)为杨柳科,杨属,落叶大乔木。该珍贵树种在海南岛霸王岭高山地区被发现,它的发现打破了杨树在我国南缘分布的纬度界限,是海南林业的一新发现。琼岛杨迁地培育新技术研究课题组,经过深入调查发现,琼岛杨分布于海南岛西部昌江和白沙两县交界处,霸王岭保护区东部子宰林场150林班、东干线13km处、大炎工区178林班的县边山、保护区内169、177、176、165林班。在鹦歌岭自然保护区内、五指山市南阳镇、布伦村委会的山上以及白沙县南开乡高峰村的原始林中,都有琼岛杨树分布。这次深入调查发现,琼岛杨群落分布的面积范围很大,种质资源非常丰富。展开更多
采用完全随机区组设计,研究了留茬高度(15、10和5 cm)及平茬时间(11月和2月)对凤丹(Paeonia ostii T. Hong et J. X. Zhang)3年生实生苗生长、光合生理和结实的影响。结果表明:随留茬高度的降低,凤丹的冠幅、单株新生枝数量、单株叶面...采用完全随机区组设计,研究了留茬高度(15、10和5 cm)及平茬时间(11月和2月)对凤丹(Paeonia ostii T. Hong et J. X. Zhang)3年生实生苗生长、光合生理和结实的影响。结果表明:随留茬高度的降低,凤丹的冠幅、单株新生枝数量、单株叶面积、比叶质量、叶片的光合作用参数[包括净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和水分利用效率(WUE)]和叶绿素含量、果荚直径、单果荚籽粒数以及百粒质量均呈逐渐增加的趋势,且明显高于对照(未平茬处理)组。在同一留茬高度,随着时间的推移,凤丹叶片的Pn、Gs和WUE值以及叶绿素含量呈逐渐降低的趋势,Tr值呈逐渐升高的趋势。11月平茬处理组凤丹植株和叶片的生长指标、叶片光合作用参数以及结实指标总体上高于2月平茬处理组。方差分析结果表明:留茬高度处理组间凤丹的植株生长指标、叶片光合作用参数和叶绿素含量以及结实指标均存在极显著差异,平茬时间处理组间凤丹的植株生长指标和结实指标总体上存在极显著差异,其他指标间均无显著差异。总体上看,留茬高度5 cm和11月平茬处理利于凤丹塑造株型和提高光合效率,能够为优产和高产提供更有利的生长条件。展开更多
基金This work was supported by the National Key R&D Program of China(2018YFD1000405)Jiangsu Modern Agricultural Industrial Technology System(JATS[2020]436)。
文摘The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy.Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors.In this study,the expression profiles of ten candidate genes,ACT1,ACT2,GAPDH,eIF1,eIF2,α-TUB,β-TUB,TBP,RNA Pol II and RP II,were calculated for a suitable reference gene selection in Paeonia ostii T.Hong et J.X.Zhang leaves under various drought stress conditions.Data were processed by the four regularly used evaluation software.A comprehensive analysis revealed that RNA Pol II was the most stable gene and eIF2 was the least stable one.In addition,the geNorm program provided the optimal choice of two reference gene combination,RNA Pol II andβ-TUB,for qRT-PCR normalization in P.ostii subjected to different drought stress levels.Our research provided convenience for gene expression analysis in P.ostii under drought stress and promoted research of effective methods to alleviate P.ostii drought stress in the future.
基金Supported by the Cooperative Project Between the People's Republic of China and the Republic of Korea
文摘[Objectives] This study was conducted to investigate the effects of different treatments on rooting and germination of Paeonia rockii T. Hong et J.J.Li seeds. [Methods] Different stratification time and temperatures and different GA_3 concentrations and treatment time were set to screen the optimum treatment method for promoting seed rooting and germination. [Results] The rooting rate was the highest when the seeds were subjected to 30 d of stratification treatment in an incubator at 25℃, and treated with 300 mg/L GA_3 for 24 h. Soaking the seeds with 300 mg/L GA_3 for 24 h combined with 30 d of stratification treatment was most beneficial to germination, and the germination rate was over 90%. [Conclusions] This study provides technical guidance and basic information for the rooting and germination of P. rockii seeds.
文摘在黑龙江省东南部地区的海林市进行紫斑牡丹[Paeonia rockii(S.G.Haw et L.A.Lauener)T.Hong et J.J.Li]苗木的栽培试验,连续3年对紫斑牡丹的生长发育表现、物候期进行了观测,采用不同防寒措施进行对比试验。结果表明,紫斑牡丹苗木部分植株地下根系不做防寒处理能正常越冬,但地上枝条冻害严重,保存率逐年降低,长势弱,每年生长量不明显;物候期与原产地相比均较晚;采用不同防寒措施对其萌芽期和始花期影响较大,对其成活率也有一定影响。
文摘琼岛杨(Populus qiongdaoensis T.Hong et P.Luo)为杨柳科,杨属,落叶大乔木。该珍贵树种在海南岛霸王岭高山地区被发现,它的发现打破了杨树在我国南缘分布的纬度界限,是海南林业的一新发现。琼岛杨迁地培育新技术研究课题组,经过深入调查发现,琼岛杨分布于海南岛西部昌江和白沙两县交界处,霸王岭保护区东部子宰林场150林班、东干线13km处、大炎工区178林班的县边山、保护区内169、177、176、165林班。在鹦歌岭自然保护区内、五指山市南阳镇、布伦村委会的山上以及白沙县南开乡高峰村的原始林中,都有琼岛杨树分布。这次深入调查发现,琼岛杨群落分布的面积范围很大,种质资源非常丰富。
文摘采用完全随机区组设计,研究了留茬高度(15、10和5 cm)及平茬时间(11月和2月)对凤丹(Paeonia ostii T. Hong et J. X. Zhang)3年生实生苗生长、光合生理和结实的影响。结果表明:随留茬高度的降低,凤丹的冠幅、单株新生枝数量、单株叶面积、比叶质量、叶片的光合作用参数[包括净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和水分利用效率(WUE)]和叶绿素含量、果荚直径、单果荚籽粒数以及百粒质量均呈逐渐增加的趋势,且明显高于对照(未平茬处理)组。在同一留茬高度,随着时间的推移,凤丹叶片的Pn、Gs和WUE值以及叶绿素含量呈逐渐降低的趋势,Tr值呈逐渐升高的趋势。11月平茬处理组凤丹植株和叶片的生长指标、叶片光合作用参数以及结实指标总体上高于2月平茬处理组。方差分析结果表明:留茬高度处理组间凤丹的植株生长指标、叶片光合作用参数和叶绿素含量以及结实指标均存在极显著差异,平茬时间处理组间凤丹的植株生长指标和结实指标总体上存在极显著差异,其他指标间均无显著差异。总体上看,留茬高度5 cm和11月平茬处理利于凤丹塑造株型和提高光合效率,能够为优产和高产提供更有利的生长条件。
