Mn-TCPP-CSn(n=6,1 1,20) as a type of potential magnetic resonance imaging(MRI) contrast agents were synthesized via manganese(Ⅱ) meso-tetra(4-carboxyphenyl) porphyrin(Mn-TCPP) modified with chitosan oligosa...Mn-TCPP-CSn(n=6,1 1,20) as a type of potential magnetic resonance imaging(MRI) contrast agents were synthesized via manganese(Ⅱ) meso-tetra(4-carboxyphenyl) porphyrin(Mn-TCPP) modified with chitosan oligosaccharides(CSn).Experimental data of infared(IR),UV-Vis,MS,inductively coupled plasma-atomic emission spectrometer(ICP-AES) and size exclusion chromatography evidenced the formation of Mn-TCPP-CSn-The stability results show that Mn-TCPP-CSn in aqueous solution was stable enough to prevent Mn(Ⅱ) ions from leaking.The magnetic properties in vitro indicate that Mn-TCPP-CS20 possesses higher longitudinal relaxivity(r1=10.38 L·mmol^-1·s^-1) in aqueous solution than unmodified porphyrin Mn-TCPPNa4[manganese(Ⅱ) meso-tetra(4-carboxyphenyl) porphyrin,tetrasodium salt](r1=5.10 L·mmol^-1·s^-1) and the commercial contrast agent Gd-DTPA(r1=4.05 L·mmol^-1·s^-1).The preliminary T1-weighted flash image studies in vitro show that the contrast and the imaging signal of Mn-TCPP-CSn were superior to those of Mn-TCPPNa4 and Gd-DTPA under the same conditions.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay shows that Mn-TCPP-CSn has a good biocompatibility.In addition,the thermodynamical parameters(ΔH〈0,ΔS〈0,ΔG〈0) of Mn-TCPP-CSn bound to bovine serum albumin(BSA) show that Mn-TCPP-CSn could bind to BSA spontaneously,where the binding complex was stabilized mainly by van der Waals interactions and hydrogen bonds.These results suggest that Mn-TCPP-CSn have the advantage of becoming a potential MRI contrast agent.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.21261008, 21302071, 21171076), and the Cooperation Project of Hainan International Science and Technology, China(No.KJHZ2014-05).
文摘Mn-TCPP-CSn(n=6,1 1,20) as a type of potential magnetic resonance imaging(MRI) contrast agents were synthesized via manganese(Ⅱ) meso-tetra(4-carboxyphenyl) porphyrin(Mn-TCPP) modified with chitosan oligosaccharides(CSn).Experimental data of infared(IR),UV-Vis,MS,inductively coupled plasma-atomic emission spectrometer(ICP-AES) and size exclusion chromatography evidenced the formation of Mn-TCPP-CSn-The stability results show that Mn-TCPP-CSn in aqueous solution was stable enough to prevent Mn(Ⅱ) ions from leaking.The magnetic properties in vitro indicate that Mn-TCPP-CS20 possesses higher longitudinal relaxivity(r1=10.38 L·mmol^-1·s^-1) in aqueous solution than unmodified porphyrin Mn-TCPPNa4[manganese(Ⅱ) meso-tetra(4-carboxyphenyl) porphyrin,tetrasodium salt](r1=5.10 L·mmol^-1·s^-1) and the commercial contrast agent Gd-DTPA(r1=4.05 L·mmol^-1·s^-1).The preliminary T1-weighted flash image studies in vitro show that the contrast and the imaging signal of Mn-TCPP-CSn were superior to those of Mn-TCPPNa4 and Gd-DTPA under the same conditions.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay shows that Mn-TCPP-CSn has a good biocompatibility.In addition,the thermodynamical parameters(ΔH〈0,ΔS〈0,ΔG〈0) of Mn-TCPP-CSn bound to bovine serum albumin(BSA) show that Mn-TCPP-CSn could bind to BSA spontaneously,where the binding complex was stabilized mainly by van der Waals interactions and hydrogen bonds.These results suggest that Mn-TCPP-CSn have the advantage of becoming a potential MRI contrast agent.