Expression of TAC1 and Its Receptors TACR1,TACR2,TACR3 Genes in Different Goat Tissues Under Different Photoperiods

This study was conducted to analyze the regulation mechanisms of TAC1,TACR1,TACR2 and TACR3 genes on reproduction of goat under different photoperiods. The expression conditions of TAC1,TACR1,TACR2 and TACR3 genes in 12 tissues( oviduct,ovary,uterus,gluteus,mesenteric fat,brain,cerebellum,medulla oblongata,hart,lung,liver,kidney) of adult Henan Huai goat under different photoperiods( short day light,Light 8 h∶ dark 16 h,and long day light,light 16 h∶ dark 8 h) were analyzed by q-PCR method. TAC1,TACR1,TACR2 and TACR3 genes were expressed in all the 12 tissues of goats,with different expression characteristics; and the expression levels of all the genes were affected by photoperiod and changing of light signal between light and dark under short photoperiod. Shifting of light signal from dark to light was more conductive to the expression of these genes in all tissues than that of light signal from light to dark.There were significant differences in the expression levels of genes between light shifting from light to dark and from dark to light when the genes were expressed at a higher level in some tissues. TAC1 and TACR2 genes were expressed at a higher level than TACR1 and TACR3 genes in the various tissues,which implied that TACR2 is a receptor given priority to combine with TAC1 when TAC1 is functional.

细胞分裂素受体基因TaCRE1变异对小麦次生根及分蘖的影响

为了探讨小麦TaCRE1基因与次生根、分蘖的关系,本研究选用40份农艺性状差异较大的小麦品种,检测和鉴定该基因等位变异,并分析其对不同时期的小麦次生根、分蘖的影响。结果表明,冬前及拔节期次生根数和分蘖数与最终有效分蘖相关性较好。TaCRE1基因等位变异对冬前及拔节期次生根和分蘖数影响显著,具有B型等位基因的品种的次生根、分蘖数的均值显著高于具有A型等位基因品种。相关分析结果表明,TaCRE1等位基因变异与冬前、拔节期次生根数、分蘖数以及有效分蘖数均值都呈显著或极显著正相关,证实TaCRE1基因等位变异对上述生长阶段次生根、分蘖发育及有效分蘖作用较大。