MCM7和TACSTD2在甲状腺乳头状癌中的表达及意义

目的:研究微小染色体维持蛋白7 (MCM7)和肿瘤相关钙信号转导蛋白2 (TACSTD2)在甲状腺乳头状癌(PTC)中的表达及临床意义。方法:采用免疫组化方法对109例甲状腺良恶性病变(甲状腺乳头状癌69例、结节性甲状腺肿组织40例、癌旁甲状腺组织69例)中MCM7和TACSTD2的表达进行检测。结果:MCM7在上述组织中的阳性表达率分别为97.1% (67/69)、65.0% (26/40)和15.94% (11/69),TACSTD2在上述组织的阳性表达率分别为98.55% (68/69)、60.0% (24/40)和17.39% (12/69)。两种蛋白在上述三种组织的阳性表达率差异均具有统计学意义(c2 = 94.5 P c2 = 93.6, P P P = 0.016, Objective: To investigate the expression and clinical significance of minosomal maintenance protein 7 (MCM7) and tumor-associated calcium signal transducer 2 (TACSTD2) in papillary thyroid carcinoma (PTC). Methods: Immunohistochemistry was used to detect the expression of MCM7 and TACSTD2 in 109 cases of benign and malignant thyroid lesions (69 cases of papillary thyroid carcinoma, 40 cases of nodular goiter and 69 cases of adjacent thyroid tissues). Results: The positive expression rates of MCM7 in the above tissues were 97.1% (67/69), 65.0% (26/40) and 15.94% (11/69), respectively. The positive expression rates of TACSTD2 in the above tissues were 98.55% (68/69), 60.0% (24/40) and 17.39% (12/69), respectively. The positive expression rates of the two proteins in the above three tissues were statistically significant (c2 = 94.5, P c2 = 93.6, P P P = 0.016, < 0.05). Conclusions: MCM7 and TACSTD2 are highly expressed in PTC tissues, and the positive expression of TACSTD2 is positively correlated with tumor diameter, which may be involved in the occurrence and development of PTC. Both of them may provide new molecular biological markers for the diagnosis of PTC.

TACSTD2在卵巢高级别浆液性癌中的表达及意义

目的探讨肿瘤关联钙信号转导因子2(TACSTD2)在卵巢高级别浆液性癌中的异常表达,揭示其与"输卵管起源"发病机制的相关性。方法收集2015年3月至2016年10月于青岛大学附属医院妇科行手术切除的卵巢高级别浆液性癌、输卵管上皮、卵巢表面上皮的组织标本各36例,通过免疫组化法检测"苗勒氏管"起源标记物配对盒基因8(PAX8)的表达,比较三组在组织学表型方面的相似性;应用光纤微珠芯片平台进行分析,比较三组在基因表达谱方面的相似性;进而筛选出与卵巢高级别浆液性癌相关程度较高的目的基因。通过实时定量聚合酶链反应(RT-PCR)及蛋白质免疫印迹(Western blot)检测目的基因在不同组织中的表达差异。结果 PAX8在输卵管上皮中的阳性表达率为100.00%(36/36),在卵巢高级别浆液性癌的阳性表达率为83.33%(30/36),在卵巢上皮中的阳性表达率为16.67%(6/36)。前两者差异无统计学意义(χ~2=2.180,P>0.05),后两者差异有统计学意义(χ~2=10.670,P<0.05),提示前两者在组织学表型上的相似性。芯片分析显示,相比较卵巢上皮,卵巢高级别浆液性癌和输卵管上皮表现出更多的基因表达谱相似性。筛选出目的基因TACSTD2,并证实其在卵巢高级别浆液性癌中的基因和蛋白表达较在输卵管上皮组织中升高,差异有统计学意义(t=-50.411,P<0.05)。结论 TACSTD2在卵巢高级别浆液性癌中的高表达,可能在输卵管起源的发病机制中发挥重要作用。

血清中肿瘤相关钙信号转导蛋白2抗体对乳腺癌诊断及预后的临床价值

目的探讨乳腺癌患者血清中肿瘤相关钙信号传导蛋白2(TACSTD2)抗体表达水平及其在乳腺癌诊断及预后中的临床应用价值。方法用表达TACSTD2的噬菌体包被空白的微孔板,ELISA法分别检测100例乳腺癌患者、60例乳腺良性增生患者及60名健康体检者血清中TACSTD2抗体含量。结果与健康对照组相比,乳腺癌患者血清中TACSTD2抗体含量明显升高,具有统计学意义(P<0.001);乳腺癌患者组血清中TACSTD2抗体含量亦明显高于良性对照组(P<0.001);但是已发生淋巴结转移的乳腺癌患者与未发生淋巴结转移的患者血清中TACSTD2抗体含量相比较,无显著性差异(P>0.05)。结论血清TACSTD2抗体的表达与乳腺癌的发生密切相关,可作为乳腺癌诊断的肿瘤标志物。