Epithelial-mesenchymal transition(EMT)is a vital pathological feature of silica-induced pulmonary fibrosis.However,whether circRNA is involved in the process remains unclear.The present study aimed to investigate the ...Epithelial-mesenchymal transition(EMT)is a vital pathological feature of silica-induced pulmonary fibrosis.However,whether circRNA is involved in the process remains unclear.The present study aimed to investigate the role of circPVT1 in the silica-induced EMT and the underlying mechanisms.We found that an elevated expression of circPVT1 promoted EMT and enhanced the migratory capacity of silica-treated epithelial cells.The isolation of cytoplasmic and nuclear separation assay showed that circPVT1 was predominantly expressed in the cytoplasm.RNA immunoprecipitation assay and RNA pull-down experiment indicated that cytoplasmic-localized circPVT1 was capable of binding to miR-497-5p.Furthermore,we found that miR-497-5p attenuated the silica-induced EMT process by targeting transcription factor 3(TCF3),an E-cadherin transcriptional repressor,in the silica-treated epithelial cells.Collectively,these results reveal a novel role of the circPVT1/miR-497-5p/TCF3 axis in the silica-induced EMT process in lung epithelial cells.Once validated,this finding may provide a potential theoretical basis for the development of interventions and treatments for pulmonary fibrosis.展开更多
针对末端夹持激光位移传感器的机器人TCF(tool/terminal control frame)标定,提出一种基于平面模扳的标定方法,机器人操作末端执行器使激光位移传感器在不同位形下对平面模板进行测量,再通过非线性最小.乘拟合求解标定参数.为了减小问...针对末端夹持激光位移传感器的机器人TCF(tool/terminal control frame)标定,提出一种基于平面模扳的标定方法,机器人操作末端执行器使激光位移传感器在不同位形下对平面模板进行测量,再通过非线性最小.乘拟合求解标定参数.为了减小问题的奇异性,对标定时应该采取的参数控制策略进行了定性分析.该标定方法只需要一块表面精度较高的平面模板,而无需其它测量仪器,标定过程简单、易操作,且易于实现自动己.仿真和实验结果表明本文提出的标定方法具有较高的精度.展开更多
基金funded by the National Natural Science Foundation of China(Grant No.82073518).
文摘Epithelial-mesenchymal transition(EMT)is a vital pathological feature of silica-induced pulmonary fibrosis.However,whether circRNA is involved in the process remains unclear.The present study aimed to investigate the role of circPVT1 in the silica-induced EMT and the underlying mechanisms.We found that an elevated expression of circPVT1 promoted EMT and enhanced the migratory capacity of silica-treated epithelial cells.The isolation of cytoplasmic and nuclear separation assay showed that circPVT1 was predominantly expressed in the cytoplasm.RNA immunoprecipitation assay and RNA pull-down experiment indicated that cytoplasmic-localized circPVT1 was capable of binding to miR-497-5p.Furthermore,we found that miR-497-5p attenuated the silica-induced EMT process by targeting transcription factor 3(TCF3),an E-cadherin transcriptional repressor,in the silica-treated epithelial cells.Collectively,these results reveal a novel role of the circPVT1/miR-497-5p/TCF3 axis in the silica-induced EMT process in lung epithelial cells.Once validated,this finding may provide a potential theoretical basis for the development of interventions and treatments for pulmonary fibrosis.
文摘针对末端夹持激光位移传感器的机器人TCF(tool/terminal control frame)标定,提出一种基于平面模扳的标定方法,机器人操作末端执行器使激光位移传感器在不同位形下对平面模板进行测量,再通过非线性最小.乘拟合求解标定参数.为了减小问题的奇异性,对标定时应该采取的参数控制策略进行了定性分析.该标定方法只需要一块表面精度较高的平面模板,而无需其它测量仪器,标定过程简单、易操作,且易于实现自动己.仿真和实验结果表明本文提出的标定方法具有较高的精度.
