The number of trichomes significantly increased in CRISPR/Cas9-edited BrrTCP4b turnip(Brassica rapa var.rapa)plants.However,the underlying molecular mechanism remains to be uncovered.In this study,we performed the Y2H...The number of trichomes significantly increased in CRISPR/Cas9-edited BrrTCP4b turnip(Brassica rapa var.rapa)plants.However,the underlying molecular mechanism remains to be uncovered.In this study,we performed the Y2H screen using BrrTCP4b as the bait,which unveiled an interaction between BrrTCP4b and BrrTTG1,a pivotal WD40-repeat protein transcription factor in the MYB-bHLH-WD40(MBW)complex.This physical interaction was further validated through bimolecular luciferase complementation and co-immunoprecipitation.Furthermore,it was found that the interaction between BrrTCP4b and BrrTTG1 could inhibit the activity of MBW complex,resulting in decreased expression of BrrGL2,a positive regulator of trichomes development.In contrast,AtTCP4 is known to regulate trichomes development by interacting with AtGL3 in Arabidopsis thaliana.Overall,this study revealed that BrrTCP4b is involved in trichome development by interacting with BrrTTG1 in turnip,indicating a divergence from the mechanisms observed in model plant A.thaliana.The findings contribute to our understanding of the regulatory mechanisms governing trichome development in the non-model plants turnip.展开更多
低速率拒绝服务LDoS(Low-rate Denial of Service)攻击是当代大数据中心和云计算平台的最大威胁之一。本文主要通过NS2仿真平台实现LDoS攻击,并利用TCP状态机模型建立HMM模型,计算TCP状态机拥塞控制四个参量求加权平均数,得到的值用NCPS...低速率拒绝服务LDoS(Low-rate Denial of Service)攻击是当代大数据中心和云计算平台的最大威胁之一。本文主要通过NS2仿真平台实现LDoS攻击,并利用TCP状态机模型建立HMM模型,计算TCP状态机拥塞控制四个参量求加权平均数,得到的值用NCPSD的值代替,作为判别有无攻击的依据,以此达到检测LDoS攻击的目的。展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences,Pan-Third Pole Environment Study for a Green Silk Road(Pan-TPE)(XDA2004010306)the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502)Science and Technology Program of Xizang Autonomous Region(XZ202001ZY0003G).
文摘The number of trichomes significantly increased in CRISPR/Cas9-edited BrrTCP4b turnip(Brassica rapa var.rapa)plants.However,the underlying molecular mechanism remains to be uncovered.In this study,we performed the Y2H screen using BrrTCP4b as the bait,which unveiled an interaction between BrrTCP4b and BrrTTG1,a pivotal WD40-repeat protein transcription factor in the MYB-bHLH-WD40(MBW)complex.This physical interaction was further validated through bimolecular luciferase complementation and co-immunoprecipitation.Furthermore,it was found that the interaction between BrrTCP4b and BrrTTG1 could inhibit the activity of MBW complex,resulting in decreased expression of BrrGL2,a positive regulator of trichomes development.In contrast,AtTCP4 is known to regulate trichomes development by interacting with AtGL3 in Arabidopsis thaliana.Overall,this study revealed that BrrTCP4b is involved in trichome development by interacting with BrrTTG1 in turnip,indicating a divergence from the mechanisms observed in model plant A.thaliana.The findings contribute to our understanding of the regulatory mechanisms governing trichome development in the non-model plants turnip.
文摘低速率拒绝服务LDoS(Low-rate Denial of Service)攻击是当代大数据中心和云计算平台的最大威胁之一。本文主要通过NS2仿真平台实现LDoS攻击,并利用TCP状态机模型建立HMM模型,计算TCP状态机拥塞控制四个参量求加权平均数,得到的值用NCPSD的值代替,作为判别有无攻击的依据,以此达到检测LDoS攻击的目的。
