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Diagnostic significance of TCR gene clonal rearrangement analysis in early mycosis fungoides 被引量:3
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作者 Chen Xu Chuan Wan +3 位作者 Lin Wang Han-Jun Yang Yuan Tang Wei-Ping Liu 《Chinese Journal of Cancer》 SCIE CAS CSCD 北大核心 2011年第4期264-272,共9页
Mycosis fungoides(MF),the most common type of cutaneous T-cell lymphoma,has various unspecific clinical and histological characteristics.Its early diagnosis is challenging.The application of T-cell receptor(TCR) gene ... Mycosis fungoides(MF),the most common type of cutaneous T-cell lymphoma,has various unspecific clinical and histological characteristics.Its early diagnosis is challenging.The application of T-cell receptor(TCR) gene clonal rearrangement to the diagnosis of MF has been widely studied.In this study,we used polymerase chain reaction(PCR) to investigate the diagnostic significance of detecting TCR-γ and-β gene clonal rearrangement in the early diagnosis of mycosis fungoides.PCR for TCR-γ and TCR-β gene rearrangement was performed on 19 patients with suspected early MF,6 with typical MF,and 6 with chronic dermatitis.Of the 19 patients with suspected early MF,13 had TCR-γ gene clonal rearrangement,whereas none had TCR-β gene clonal rearrangement.All patients with typical MF had TCR gene clonal rearrangement,in which 4 showed TCR-γ clonal rearrangement,1 showed TCR-β gene clonal rearrangements,and 1 showed both.No patients with chronic dermatitis had TCR gene clonal rearrangement.These results indicate that TCR gene clonal rearrangement analysis is a useful tool in diagnosing early MF.TCR-γ gene is recommended to the routine analysis,whereas TCR-β gene has potential in combination toward intractable cases. 展开更多
关键词 基因重排 基因克隆 早期诊断 诊断意义 tcr R基因 肉芽肿 T细胞受体
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Detection of Immunoglobulin Heavy China and T Cell Receptorγ Gene Rearrangement in Lymph Node Aspirates with PCR:Diagnostic Significance for Lymphoproliferative Disorders
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作者 盛瑞兰 《The Journal of Biomedical Research》 CAS 1994年第1期49-52,共4页
Lymph node aspirates of 17 cases with enlarged superficial or isceral lymph nodes were detected for immunoglobulin heavy chain gene rearrangement (IgHRA) and T cell rcceptor γ gene rearrangement (TCRγRA) bypolymeras... Lymph node aspirates of 17 cases with enlarged superficial or isceral lymph nodes were detected for immunoglobulin heavy chain gene rearrangement (IgHRA) and T cell rcceptor γ gene rearrangement (TCRγRA) bypolymerase chain reaction (PCR). Combining with clinical data, pathologic diagnosis and immunophenotapy, we analyse the results as follows: 5 cases with nonlymphoid cancers and 3 cases with reactive lymphadenopathy do not present two kinds of clone gene rearrangements.5 out of 7 cases with NHL show clone gene rearrangements (IgH 3 cases, TCRY 2cases),two kinds of monoclonal band(100-120bp for IgHRA and 170-230bp for TCRγRA) were observed after electrophoresis of amplified DNA products. One case whose clinical sitcaion accorded with features of lymphoma was diagnosed as granulomatous lymphadenitis by pathologist, after gene rearaangement clone TCRγRA was detected,a correct diaguosis as NHL was made then. The significance of detecting the two kinds of gene rearrangement for clinical application and the limitations in diagnosis of lymphoproliferative disorders was discussed. 展开更多
关键词 PCR IgH and tcrγ gene rearrangement lymph node asprate lymphoproliferative Disorders
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Clonal immunoglobulin heavy chain and T-cell receptor γ gene rearrangements in primary gastric lymphoma 被引量:3
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作者 Guo-Dong Shan Feng-Ling Hu +6 位作者 Ming Yang Hong-Tan Chen Wen-Guo Chen Yun-Gui Wang Li-Hua Chen You-Ming Li Guo-Qiang Xu 《World Journal of Gastroenterology》 SCIE CAS 2013年第34期5727-5731,共5页
AIM:To study the diagnostic value of immunoglobulin heavy chain(IgH)and T-cell receptorγ (TCR-γ)gene monoclonal rearrangements in primary gastric lymphoma(PGL).METHODS:A total of 48 patients with suspected PGL at ou... AIM:To study the diagnostic value of immunoglobulin heavy chain(IgH)and T-cell receptorγ (TCR-γ)gene monoclonal rearrangements in primary gastric lymphoma(PGL).METHODS:A total of 48 patients with suspected PGL at our hospital were prospectively enrolled in this study from January 2009 to December 2011.The patients were divided into three groups(a PGL group,a gastric linitis plastica group,and a benign gastric ulcer group)based on the pathological results(gastric mucosal specimens obtained by endoscopy or surgery)and follow-up.Endoscopic ultrasonography(EUS)and EUSguided biopsy were performed in all the patients.The tissue specimens were used for histopathological examination and for IgH and TCR-γ gene rearrangement polymerase chain reaction analyses.RESULTS:EUS and EUS-guided biopsy were successfully performed in all 48 patients.In the PGL group(n=21),monoclonal IgH gene rearrangements were detected in 14(66.7%)patients.A positive result for each set of primers was found in 12(57.1%),8(38.1%),and 4(19.0%)cases using FR1/JH,FR2/JH,and FR3/JH primers,respectively.Overall,12(75%)patients with mucosal-associated lymphoid tissue lymphoma(n=16)and 2(40%)patients with diffuse large B-cell lymphoma(n=5)were positive for monoclonal IgH gene rearrangements.No patients in the gastric linitis plastica group(n=17)and only one(10%)patient in the benign gastric ulcer group(n=10)were positive for a monoclonal IgH gene rearrangement.No TCRgene monoclonal rearrangements were detected.The sensitivity of monoclonal IgH gene rearrangements was 66.7%for a PGL diagnosis,and the specificity was96.4%.In the PGL group,8(100%)patients with stage IIE PGL(n=8)and 6(46.1%)patients with stage IE PGL(n=13)were positive for monoclonal IgH gene rearrangements.CONCLUSION:IgH gene rearrangements may be associated with PGL staging and may be useful for the diagnosis of PGL and for differentiating between PGL and gastric linitis plastica. 展开更多
关键词 IMMUNOGLOBULIN heavy chain T-CELL receptor γ gene rearrangement Primary gastric lymphoma Endoscopic BIOPSY specimen
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REARRANGEMENT AND EXPRESSION OF T CELL RECEPTOR β GENE IN HUMAN HEMOPOIETIC CELL LINES AND PRIMARY CELLS FROM ACUTE LYMPHOCYTIC LEUKEMIAS 被引量:2
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作者 仇一华 陈诗书 《Medical Bulletin of Shanghai Jiaotong University》 CAS 1992年第1期63-69,共7页
Using Southern blot, Northern blot and Quick blot methods, we examined the rearrangement and expression of TCR βgene in four early differentiation stage cell lines from human hemopoietic system, namely HL-60, Jurkat,... Using Southern blot, Northern blot and Quick blot methods, we examined the rearrangement and expression of TCR βgene in four early differentiation stage cell lines from human hemopoietic system, namely HL-60, Jurkat, Daudi and Raji cells as well as lymphocytes from 17 acute lymphocytic leukemia (ALL) patients. The results showed. Ⅰ) Rearrangement of TCR βgene was seen in Jurkat cells. A germline pattern was observed in HL-60, Daudi and Raji cells. 2) Eight of 9 patients with T-ALL had cells with rearranged TCR βgene. But two of 3 patients with B-ALL and three of 5 patients with nonT, nonB-ALL also had cells with rearranged TCR βgene. 3) A 1.3 kb full-length transcript and a 1.0 kb truncated transcript were detected in Jurkat cells by probing with <sup>32</sup>P-TCR βcDNA. But some leukemic B cells also expressed an incompleted transcript. 4) TCR βmRNA was detected in six of 8 patients with T-ALL, four of 5 patients with nonT, nonB-ALL and one of 3 patients with B-ALL. But the level of expression was quite differ ent. The dual-rearrangement and the abnormal expression may give us a new clue for researching leukemogenesis. 展开更多
关键词 tcr β gene rearrangement tcr β gene EXPRESSION acute LYMPHOCYTIC leukemia HUMAN hemopoietic cell lines
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Comparison of Biolistic and Agrobacterium-mediated Transformation Methods on Transgene Copy Number and Rearrangement Frequency in Rice 被引量:11
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作者 程在全 黄兴奇 Ray WU 《Acta Botanica Sinica》 CSCD 2001年第8期826-833,共8页
Transferring foreign DNA into plant cells by biolistic and Agrobacterium _mediated methods may result in random integration of different copy numbers of the transgene, and different proportions of intact vs. rearra... Transferring foreign DNA into plant cells by biolistic and Agrobacterium _mediated methods may result in random integration of different copy numbers of the transgene, and different proportions of intact vs. rearranged copies of the transgene. This may, in turn, affect transgene expression levels. To test the above hypothesis, we first introduced the same plasmid, pAc1PG_CAM, into rice (Oryza sativa L.) calli separately by the biolistic method and by the Agrobacterium _mediated method. To show whether different plasmids may affect the results, we also introduced pTOK233 by the Agrobacterium _mediated method and pJPM44 by the biolistic method. Transgene expression of R0 plants was monitored by histochemical analysis of GUS activity. Transgene copy number was determined by Southern blot analysis after digesting genomic DNA with an enzyme that has a unique cutting site within the input plasmid. The total genomic DNA was also digested by a two_cut enzyme (the cuts are located at two sides of a given transgene expression cassette), followed by Southern blotting analysis, for determining the number of intact transgene expression cassettes. Our data showed that Agrobacterium _mediated transformation resulted in lower transgene copy number (average between 2.1 and 2.3) in transgenic rice plants, compared with those plants obtained by the biolistic method (average between 4.2 and 5.6). The frequency of DNA rearrangement in expression cassettes is lower in transgenic rice plants obtained by the Agrobacterium _ mediated method than those obtained by the biolistic method. The average rearrangement frequency is 0.07 to 0.106 for the Agrobacterium _mediated method, and 0.57 to 0.66 for the biolistic method. Our results suggest that it is better to compare the number of intact expression cassettes instead of the total copy number of the transgene in demonstrating their influence on the level of transgene expression. This is the first report on the frequency of expression cassette rearrangement in transgenic plants transformed with the same plasmid by two different transformation methods. 展开更多
关键词 DNA rearrangement gene expression cassette Agrobacterium _mediated method biolistic method rice ( Oryza sativa )
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Three New Ranidae Mitogenomes and the Evolution of Mitochondrial Gene Rearrangements among Ranidae Species 被引量:1
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作者 Jiandong YANG Jiaojiao YU +3 位作者 Jiabin LIU Ming ZHOU Biao LI Bo OUYANG 《Asian Herpetological Research》 SCIE CSCD 2018年第2期85-98,共14页
Various types of gene rearrangements have been discovered in the mitogenoes of the frog family Ranidae. In this study, we determined the complete mitogenome sequence of three Rana frogs. By combining the available mit... Various types of gene rearrangements have been discovered in the mitogenoes of the frog family Ranidae. In this study, we determined the complete mitogenome sequence of three Rana frogs. By combining the available mitogenomic data sets from GenBank, we evaluated the phylogenetic relationships of Ranidae at the mitogenome level and analyzed mitogenome rearrangement cases within Ranidae. The three frogs shared an identical mitogenome organization that was extremely similar to the typical Neobatrachian-type arrangement. Except for the genus Babina, the monophyly of each genus was well supported. The genus Amnirana occupied the most basal position among the Ranidae. The [Lithobates + Rana] was the closest sister group of Odorrana. The diversity of mitochondrial gene arrangements in ranid species was unexpectedly high, with 47 mitogenomes from 40 ranids being classified into 10 different gene rearrangement types. Some taxa owned their unique gene rearrangement characteristics, which had significant implication for their phylogeny analysis. All rearrangement events discovered in the Ranidae mitogenomes can be explained by the duplication and random loss model. 展开更多
关键词 mitochondrial genomes gene rearrangement molecular phylogeny RANIDAE
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Progress in clinical diagnosis and treatment of colorectal cancer with rare genetic variants
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作者 Shuyi Chen Jing Gu +2 位作者 Kaichun Wu Xiaodi Zhao Yuanyuan Lu 《Cancer Biology & Medicine》 SCIE CAS CSCD 2024年第6期473-483,共11页
Targeted therapy is crucial for advanced colorectal cancer(CRC) positive for genetic drivers. With advances in deep sequencing technology and new targeted drugs, existing standard molecular pathological detection syst... Targeted therapy is crucial for advanced colorectal cancer(CRC) positive for genetic drivers. With advances in deep sequencing technology and new targeted drugs, existing standard molecular pathological detection systems and therapeutic strategies can no longer meet the requirements for careful management of patients with advanced CRC. Thus, rare genetic variations require diagnosis and targeted therapy in clinical practice. Rare gene mutations, amplifications, and rearrangements are usually associated with poor prognosis and poor response to conventional therapy. This review summarizes the clinical diagnosis and treatment of rare genetic variations, in genes including erb-b2 receptor tyrosine kinase 2(ERBB2), B-Raf proto-oncogene, serine/threonine kinase(BRAF), ALK receptor tyrosine kinase/ROS proto-oncogene 1, receptor tyrosine kinase(ALK/ROS1), neurotrophic receptor tyrosine kinases(NTRKs), ret proto-oncogene(RET), fibroblast growth factor receptor 2(FGFR2), and epidermal growth factor receptor(EGFR), to enhance understanding and identify more accurate personalized treatments for patients with rare genetic variations. 展开更多
关键词 genetic variation gene mutation gene amplification gene rearrangement targeted therapy
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Epidemiological Surveillance: Genetic Diversity of Rotavirus Group A in the Pearl River Delta, Guangdong, China in 2019
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作者 JIANG Jie Ying LIANG Dan +9 位作者 WANG Li XIAO Yun LIANG Yu Feng KE Bi Xia SU Juan XIAO Hong WANG Tao ZOU Min LI Hong Jian KE Chang Wen 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2024年第3期278-293,共16页
Objective This study aimed to understand the epidemic status and phylogenetic relationships of rotavirus group A(RVA)in the Pearl River Delta region of Guangdong Province,China.Methods This study included individuals ... Objective This study aimed to understand the epidemic status and phylogenetic relationships of rotavirus group A(RVA)in the Pearl River Delta region of Guangdong Province,China.Methods This study included individuals aged 28 days–85 years.A total of 706 stool samples from patients with acute gastroenteritis collected between January 2019 and January 2020 were analyzed for 17 causative pathogens,including RVA,using a Gastrointestinal Pathogen Panel,followed by genotyping,virus isolation,and complete sequencing to assess the genetic diversity of RVA.Results The overall RVA infection rate was 14.59%(103/706),with an irregular epidemiological pattern.The proportion of co-infection with RVA and other pathogens was 39.81%(41/103).Acute gastroenteritis is highly prevalent in young children aged 0–1 year,and RVA is the key pathogen circulating in patients 6–10 months of age with diarrhea.G9P[8](58.25%,60/103)was found to be the predominant genotype in the RVA strains,and the 41 RVA-positive strains that were successfully sequenced belonged to three different RVA genotypes in the phylogenetic analysis.Recombination analysis showed that gene reassortment events,selection pressure,codon usage bias,gene polymorphism,and post-translational modifications(PTMs)occurred in the G9P[8]and G3P[8]strains.Conclusion This study provides molecular evidence of RVA prevalence in the Pearl River Delta region of China,further enriching the existing information on its genetics and evolutionary characteristics and suggesting the emergence of genetic diversity.Strengthening the surveillance of genotypic changes and gene reassortment in RVA strains is essential for further research and a better understanding of strain variations for further vaccine development. 展开更多
关键词 Infectious diarrheal disease ROTAVIRUS Phylogenetic analysis gene rearrangement Codon usage bias genetic diversity
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Gene Rearrangement Analysis of Orbital Lymphoid Infiltrating Disorders 被引量:2
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作者 JianhuaYan ZhongyaoWu 《眼科学报》 2000年第1期15-21,共7页
Purpose:To determine whether the use of polymerase chain reaction for B-cell gene rearrangement in patients with orbital lymphoid infiltrate disorders could be useful in the diagnosis of lymphoma,especially,in differe... Purpose:To determine whether the use of polymerase chain reaction for B-cell gene rearrangement in patients with orbital lymphoid infiltrate disorders could be useful in the diagnosis of lymphoma,especially,in differentiating benign lesion from malignant one.Methods:In addition to clinical,pathological,and immunohistochemical evaluations,48 cases of orbital lymphoid infiltrate disorders were examined for immunoglobulin heavy(IgH)gene rearrangement by means of PCRto amplify the FR3region with formalin-fixed and paraffin-embedded tissues.Results:Gene rearrangement in the third frame-work of the IgH region was detected in specimens obtained from15cases of malignant lymphoma,4of reactive lymphoid hyperplasia and3of orbital pseudotumor.All of these patients showed a discrete band(100bp)which reflected monoclonal proliferation of B lymphocytes.5cases of malignant lymphoma,6ofreactive lymphoid hyperplasia and 15of orbital pseudotumor did not show a discrete band on PCR.Conclusions:The FR3 region gene rearrangement of Ig heavy in patients with orbital lymphoid infiltrate disorders may be an additional diagnostic tool in differentiating benign from malignant lymphoid diseases and in offering a useful adjunct for diagnosis in difficult or unclear cases.It is a reliable and practical method of gene diagnosis in orbital lymphoid infiltrate disorders and helps to identify the molecular mechanism of malignant lymhoma.Eye Science2000;16:15-21. 展开更多
关键词 眼窝 淋巴渗透紊乱 基因重组 聚合酶链反应 B淋巴细胞 细胞增殖 诊断
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THE RELATIONSHIP BETWEEN NON-HODGKIN'S LYMPHOMA AND THE GENE REARRANGEMENT
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作者 郭素堂 刘永昌 孙俊宁 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1998年第4期73-76,共4页
Objective: To investigate the pattern of clonal rearrangement of immunoglobulin heavy chain gene (IGH) and T cell receptor γ gene (TCRγ) of Non Hodgkin's lymphoma (NHL) Methods: Bone marrow smears of 211 pat... Objective: To investigate the pattern of clonal rearrangement of immunoglobulin heavy chain gene (IGH) and T cell receptor γ gene (TCRγ) of Non Hodgkin's lymphoma (NHL) Methods: Bone marrow smears of 211 patients of NHL were detected by PCR, the rearranged IGH and TCRγ gene was amplified using oligonucleotide primers Results: The clonal rearrangement of IGH gene was detectable in 51 2% (108/211); the clonal rearrangement of TCRγ gene was detectable in 21 3% (45/211); both IGH and TCRγ was detectable in 5 7% (12/211); no clonal rearrangement in 21 8% (46/211) And compared clonal gene rearrangement with pathological type and primary site of tumor Ten patients of NHL were investigated serially 5/10 patients still had clonal gene rearrangement at clinical complete remission Conclusion: It demonstrated that this assay may be useful in monitoring the minimal residual disease (MRD) and in evaluating effectiveness of therapy 展开更多
关键词 NHL Clonal gene rearrangement Minimal residual disease IGH tcrγ
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Bcl-2 GENE REARRANGEMENT DETERMINED BY PCR AS AMEAN TO DETECT MINIMAL RESIDUAL DISEASE INMALIGNANT LYMPHOMAS
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作者 向直富 卢玉英 +3 位作者 赖永榕 陈燕 李慧玉 邹萍 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1999年第1期49-52,共4页
Objective: To develop a sensitive method to detect minimal residual disease and to elucidate the significance of bcl-2 gene rearrangement in diagnosis and treatment of malignant lymphoma. Methods: Using polymerase cha... Objective: To develop a sensitive method to detect minimal residual disease and to elucidate the significance of bcl-2 gene rearrangement in diagnosis and treatment of malignant lymphoma. Methods: Using polymerase chain reaction (PCR) to detect bcl-2 gene rearrangement and using serial dilution method to define the sensitivity of PCR. Results: In 9 different malignant lymphoma cell lines, Su-DHL-4 and Su-DHL-6 were shown bcl-2(MBR)/JH rearrangement, the sensitivity of PCR was 1:105. In 16 patients with follicular lymphoma, the peripheral blood and bone marrow were PCR positive in 4 cases both at initial diagnosis and after complete remission. Conclusion: Detection of bcl-2 gene rearrangement by PCR provides a sensitive and specific assay of minimal residual disease. It is helpful to improve staging of disease, prognosis and evaluation of the treatment results. 展开更多
关键词 LYMPHOMA Polymerase chain reaction Bcl-2 gene gene rearrangement
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Detection of IgH and T-cell receptor gene rearrangement in leukemia by PCR amplification and its clinical application
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作者 徐兵 周淑芸 孙竞 《Journal of Medical Colleges of PLA(China)》 CAS 1997年第3期200-203,共4页
PCR amplification was used to detect immunoglobulin heavy chain (IgH), T-cell receptor (TCR)Vγ1-Jγ, Vδ2-Dδ 3 rearranged genes in 45 cases of acute lymphoblastic leukemia (ALL) and IgH gene rearrangement in 41 case... PCR amplification was used to detect immunoglobulin heavy chain (IgH), T-cell receptor (TCR)Vγ1-Jγ, Vδ2-Dδ 3 rearranged genes in 45 cases of acute lymphoblastic leukemia (ALL) and IgH gene rearrangement in 41 cases acute nonlymphoblastic leukemia (ANLL). The positive percentage of the IgH, TCR Vγ1-Jγ and Vδ 2-Dδ3 gene rearrangement was found to be 66. 7%, 62.2%, and 35. 6%, respectively in 45 ALL patients. Multiple rearranged IgH gene bands were found in 8 (26. 7% ) out of 30 ALL patients with IgH gene rearrangement. IgH gene rearrangemens were found in 7 (17. 1% ) out of 41 ANLL patients. ALL patients with positive IgH rearranged gene were with B cell and those with positive TCR Vγ1-Jγ gene rearrangements and with negative IgH rearranged gene were with T-ALL,those with positive both IgH and TCR Vδ2-Dδ3 rearranged genes mostly were with preB-ALL. The complete remission (CR) rate was low in ALL patients with multiple rearranged heavy chains and ANLL patients with IgH gene rearrangement, who were more likely to have a clinical relapse, which may be due to clonal evolution. In complete remission (CR) patients, the cytologic relapse fell behind postive PCR results for an average periods of 7. 2 weeks (range 2 to 23 weeks). Using PCR to detect rearranged genes could be helpful in determining the efficacy of chemotherapy, predicting clinical relapse, assisting gene typing and predicting prognosis. 展开更多
关键词 POLYMERASE CHAIN reaction (PCR) LEUKEMIA rearranged geneS multiple rearrangementS
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T-CELL RECEPTOR GENE REARRANGEMENT ANALYSIS IN THE PRIMARY CUTANEOUS T-CELL LYMPHOMA
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作者 邱丙森 王平 +2 位作者 高红阳 尚易非 许良中 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1997年第3期53-58,共6页
Object: The present paper is to evaluate the significance of T cell receptor (TCR) gene rearrange ments in primary cutaneous T cell lymphomas (PCTCL) as detected by analysis of Southern Blot (SBA) and polymerase c... Object: The present paper is to evaluate the significance of T cell receptor (TCR) gene rearrange ments in primary cutaneous T cell lymphomas (PCTCL) as detected by analysis of Southern Blot (SBA) and polymerase chain reaction (PCR). Patients and Methods: Skin specimens and peripheral blood samples were taken from 44 patients with PCTCL, including 30 patients with mycosis fungoides (MF), 2 patients with Sezary's syndrome (SS), and 12 patients with PCTCL other than MF and SS (PNCTCL). 11 patients with a presumptive diagnosis of MF, 23 patients with lymphoproliferative dermatoses including lymphomatoid papulosis (LyP) and 8 patients with benign cutaneous lymphoid infiltrates were simultaneously studied by the amplification of junctional V (variable) J (joining) sequences of the rearranged TCRγ genes by PCR(TCRγPCR) and the analysis of TCRb chain genes by SBA(TCRβSBA) for detection of clonal gene rearrangements (GR). One lymph node specimen of a case with MF IIA was also detected by TCRγ PCR and TCRβSBA. Results: In MF, GR were detected by TCRγPCR and TCRβSBAb in 83.3 85.7% and 66.7% 71.4% of skin specimens of cases IIA IIB and in 57.1% 70.0% and 14.3% 10.0% of those of cases IA IB, respectively. GR were seen in 66.7% 71.4% and 33.3% 43.0.% of blood samples of cases IIA IIB, and 42.9% 40.0% and 0 10.0% of those of cases IA IB, respectively. GR was confirmed by TCRγ PCR and TCRβSBA in one lymph node showing dermato pathic lymphadenopathy of a case with MF IIA. In 11 patients of clinically suspected MF, GR were present in skin specimens of 5 cases (45.4%) and in blood samples of 3 cases ( 27.3% ) by TCRγ PCR. In PNCTCL, GR were found in 9 skin specimens (90.0%) from 10 patients detected by TCRγ PCR and in 6 skin specimens (75.0%) from 8 patients detected by TCRβSBA. GR were also seen in 6 blood samples (72.8%) from 11 patients detected by TCRγ PCR, and in 7 blood samples (70.0%) from 10 patients by TCRβSBA. In SS and LyP, GR were detected by TCRγ PCR and TCRβSBA in each of the two skin specimens of two cases with LyP and in each of the two blood samples of two cases with SS. GR were seen in one skin specimen of one case with SS and one blood sample of one case with LyP detected by TCRγPCR. Conclusions: This study demonstrated that TCRγ PCR is a rapid, more sensitive tool than TCRβSBA, can be used in the analysis of T cell clonality in skin, lymph node and blood samples of patients with PCTCL and indicated that this method forms a useful supplement to other methods for diagnosis of early and suspected MF, confirmation of PNCTCL and determination of extracutaneous involvement of lymph node and blood. 展开更多
关键词 Primary cutaneous T cell lymphoma PCR T cell receptor gene rearrangement.
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STUDY ON THE RFLPs AND AMPLICATION AND REARRANGEMENT OF THE TRANSFORMING GENES IN PRIMARY HEPATIC CARCINOMA, GASTRIC CARCINOMA AND BRAIN TUMOR WITH SIX HUMAN ONCOGENE PROBES
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作者 王世浚 单祥年 +10 位作者 张丽珊 高翼之 赵寿元 张志平 李方园 张芹 严明 黄鹰 茅一萍 蒋清 贺林 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1990年第2期22-26,共5页
By using c-Ha-ras-1, N-ras Wigler (left sequence) and P52C.(right sequence), c-sis, v-erbB, c-myc and v-fos oncogenes as probes, restriction fragment length polymorphisms (RFLPs) of tumor tissue DNAs of 95 patients wi... By using c-Ha-ras-1, N-ras Wigler (left sequence) and P52C.(right sequence), c-sis, v-erbB, c-myc and v-fos oncogenes as probes, restriction fragment length polymorphisms (RFLPs) of tumor tissue DNAs of 95 patients with gastric carcinoma, primary hepatic carcinoma and brain tumor, and those of 90 normal individuals were studied with the techniques of Southern blot and dot blot. Gene amplification and recombination were also examined in some tumors simultaneously. Some alleles of oncogene are reported in Chinese population for the first time. Moreover, the characteristic frequency of some "rare" alleles and genotypes occurred in some tumor samples is significantly higher than that occured in normal individuals. Pedigree analysis for 2 patients showed that some "rare" alleles are also abandant. Besides, gene amplification and recombination were found in some tumors. 展开更多
关键词 STUDY ON THE RFLPs AND AMPLICATION AND rearrangement OF THE TRANSFORMING geneS IN PRIMARY HEPATIC CARCINOMA GASTRIC CARCINOMA AND BRAIN TUMOR WITH SIX HUMAN ONCOgene PROBES gene
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Detection of BCL2-IGHrearrangement on paraffin-embedded tissue sections obtained from a small submucosal tumor of the rectum in a patient with recurrent follicular lymphoma 被引量:3
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作者 Naohisa Yoshida Kenichi Nomura +7 位作者 Yosuke Matsumoto Kazuhiro Nishida Naoki Wakabayashi Hideyuki Konishi Shoji Mitsufuji Keisho Kataoka Takeshi Okanoue Masafumi Taniwaki 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第17期2602-2604,共3页
A 59-year-old woman was admitted to our hospital because of recurrent follicular lymphoma(FL).Colonoscopic examination revealed a rectal submucosal tumor(SMT)without any erosions and ulcers.In this patient,it was diff... A 59-year-old woman was admitted to our hospital because of recurrent follicular lymphoma(FL).Colonoscopic examination revealed a rectal submucosal tumor(SMT)without any erosions and ulcers.In this patient,it was difficult to distinguish non-Hodgkin's lymphoma(NHL)invasion from other disorders of the colon including carcinoid tumor merely based on endoscopic findings.Histopathologic and immunohistochemical studies on biopsy specimens showed an infiltration of atypical lymphocytes that were positive for CD20 and BCL2 but negative for UCHL-1.Fluorescence in situ hybridization on paraffin-embedded tissue sections (T-FISH)identified a translocation of BCL2 with IGHgene. Based on these findings,the tumor was defined as an invasion of FL.T-FISH method is useful for the detection of a monoclonality of atypical lymphocytes in an SMT of the gastrointestinal tract,and particularly for the detection of chromosomal translocations specific to lymphoma subtypes. 展开更多
关键词 gene rearrangement BIOPSY Female Humans Immunoglobulin Heavy Chains In Situ Hybridization Fluorescence Lymphoma Follicular Middle Aged Neoplasm Recurrence Local Paraffin Embedding Proto-Oncogene Proteins c-bcl-2 Rectal Neoplasms
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BCL-1 Rearrangement in Acute Lymphocytic Leukemia and Its Clinical Significance
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作者 刘新月 唐泽海 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2001年第4期283-285,共3页
BCL 1 rearrangement (BCL 1/IgH gene rearrangement) in acute lymphocytic leukemia and its clinical significance was investigated. In 38 patients with acute lymphocytic leukemia (ALL), the genomic DNA of mononuclear c... BCL 1 rearrangement (BCL 1/IgH gene rearrangement) in acute lymphocytic leukemia and its clinical significance was investigated. In 38 patients with acute lymphocytic leukemia (ALL), the genomic DNA of mononuclear cells isolated from peripheral blood and bone marrow was amplified by using hemi nested polymerase chain reaction (PCR) technique and the expression of cycline D1 protein of mononuclear cells was detected by using immunohistochemical method. Ten patients with acute granulocytic leukemia, 2 with chronic granulocytic leukemia and 10 with normal bone marrow served as control group. The results showed that BCL 1 rearrangement was detectable in 3 of 38 ALL patients (7.9 %) and cyclin D1 protein positive expression was detected in 4 ALL patients (10 5 %). Three ALL patients with BCL 1 rearrangement were all B cell leukemia (B ALL) and accompanied by cyclin D1 protein expression. No BCL 1/IgH rearrangement or cyclin D1 protein expression was detected in 12 patients with granulocytic leukemia and 10 cases of normal bone marrow. Leukocyte counts in peripheral blood of B ALL patients with BCL 1 rearrangement and(or) cyclin D1 protein expression were significantly increased and the patients had bad reaction to chemotherapy. It was concluded that: 1) BCL 1/IgH gene rearrangement were detected in acute B lymphocytic leukemia; 2) B ALL patients with BCL 1 rearrangement and(or) cyclin D1 protein expression had poor prognosis. 展开更多
关键词 LEUKEMIA gene rearrangement BCL 1 protein cyclin D1 protein
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Two complete mitogenomes of Ocypodoidea(Decapoda: Brachyura), Cleistostoma dilatatum(Camptandriidae) and Euplax sp.(Macrophthalmidae) and its phylogenetic implications
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作者 Ying Zhang Liming Wei +3 位作者 Bingjian Liu Liqin Liu Zhenming Lü Li Gong 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2023年第4期81-92,共12页
Complete mitochondrial genomes(mitogenomes) can provide useful information for phylogenetic relationships,gene rearrangement, and molecular evolution. In the present study, two newly sequenced mitogenomes of Ocypodoid... Complete mitochondrial genomes(mitogenomes) can provide useful information for phylogenetic relationships,gene rearrangement, and molecular evolution. In the present study, two newly sequenced mitogenomes of Ocypodoidea(Cleistostoma dilatatum and Euplax sp.) were reported for the first time, which are 15 444 bp and16 129 bp in length, respectively. Cleistostoma dilatatum is the first species in the family Camptandriidae whose complete mitogenome was sequenced. Each mitogenome contains an entire set of 37 genes and a putative control region, but their gene arrangements are largely different. Tandem duplication and random loss model is proposed to account for their gene arrangements. Comparative genomic analyses of 19 mitogenomes clustering in one branch reveal that 18 of them shared the same gene rearrangement, while that of C. dilatatum mitogenome was consistent with the ancestral gene arrangement of Brachyura. The dN/dS ratio analysis shows that all PCGs are evolving under purifying selection. Phylogenetic analyses show that all Macrophalmidae species cluster together as a group, and then form a sister clade with Camptandriidae. Moreover, the polyphyly of three superfamilies(Ocypodoidea, Eriphioidea, and Grapsoidea) is reconfirmed. These findings help to confirm the phylogenetic position of Camptandriidae, as well as provide new insights into the phylogeny of Brachyura. 展开更多
关键词 Camptandriidae Macrophthalmidae MITOGENOME gene rearrangement phylogenetic analysis
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Comparative mitochondrial genome analysis of Cynoglossidae(Teleost:Pleuronectiformes)and phylogenetic implications
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作者 Bilin Hu Tingqi Jiang +7 位作者 Liming Wei Nannan Zhang Kaixin Wang Liqin Liu Bingjian Liu Jing Liu Zhenming Lü Li Gong 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2023年第11期69-80,共12页
Generally,a teleostean group(e.g.,family or genus)owns one type or a set of similar mitochondrial gene arrangement.It is interesting,however,that four different types of gene arrangement have been found in the mitocho... Generally,a teleostean group(e.g.,family or genus)owns one type or a set of similar mitochondrial gene arrangement.It is interesting,however,that four different types of gene arrangement have been found in the mitochondrial genome(mitogenome)of Cynoglossidae species.So far,the possible mechanisms of mitogenomic gene rearrangement and its potential implications have aroused widespread attention and caused lots of controversy.Here,a total of 21 Cynoglossidae mitogenomes and a newly sequenced mitogenome of Cynoglossus puncticpes(Pleuronectiformes:Cynoglossidae)were compared.The length ranges from 16417 bp to 18369 bp,which is mainly caused by the length heteroplasmy of control region(CR).Further analysis reveals that the difference of tandem repeats acts as a determining factor resulting in the length heterogeneity.Like most gene rearrangements of Cynoglossinae mitogenomes,tRNA-Gln gene encoded by the L-strand has translocated to the H-strand(Q inversion),accompanied by the translocation of CR in C.puncticpes mitogenome.The typical IQM order(tRNA-Ile-Gln-Met)changed to QIM order.Tandem duplication/random loss and mitochondrial recombination were accepted as the most possible models to account for the rearrangements in C.puncticpes mitogenome.Phylogenetic trees showed a strong correlation between the gap spacer in the rearranged QIM area and phylogeny,which provides a fresh idea for phylogenetic studies in future. 展开更多
关键词 MITOGENOME gene rearrangement tandem duplication/random loss mitochondrial inversion control region phylogenetic study
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唾液腺腺泡细胞癌中NR4A3基因重排及NOR-1蛋白表达分析
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作者 王敏 钱佳骏 +4 位作者 薛俊青 顾挺 胡宇华 陈颖 夏荣辉 《中国口腔颌面外科杂志》 CAS 2024年第3期249-254,共6页
目的:探讨NR4A3基因重排和NOR-1蛋白表达在唾液腺腺泡细胞癌中的表达及在鉴别诊断中的价值。方法:收集2020年5月—2024年1月于上海交通大学医学院附属第九人民医院口腔病理科诊断的唾液腺癌119例,包括腺泡细胞癌(acinic cell carcinoma,... 目的:探讨NR4A3基因重排和NOR-1蛋白表达在唾液腺腺泡细胞癌中的表达及在鉴别诊断中的价值。方法:收集2020年5月—2024年1月于上海交通大学医学院附属第九人民医院口腔病理科诊断的唾液腺癌119例,包括腺泡细胞癌(acinic cell carcinoma,AciCC)63例,黏液表皮样癌(mucoepidermoid carcinoma,MEC)31例,分泌性癌(secretory carcinoma,SC)25例。分别使用荧光原位杂交和免疫组织化学染色检测NR4A3基因重排和NOR-1蛋白表达情况,采用SPSS 18.0软件包对数据进行统计学分析。结果:AciCC主要发生于大唾液腺。与MEC和SC相比,AciCC好发于女性(P=0.006)。NR4A3基因重排在AciCC、MEC和SC中的阳性率分别为76.2%(48/63)、0%(0/10)和0%(0/7),NOR-1蛋白表达在AciCC、MEC和SC中的阳性率分别为92.1%(58/63)、9.7%(3/31)和0%(0/25),差异具有统计学意义(P<0.001)。单独使用NR4A3基因重排诊断AciCC时,灵敏度和特异度分别为76.2%和100%。单独使用NOR-1蛋白表达诊断AciCC时,灵敏度和特异度分别为92.1%和94.6%。联合使用NR4A3基因重排和NOR-1蛋白表达诊断AciCC时,灵敏度和特异度分别为96.8%和94.6%,曲线下面积为0.896,诊断价值最优。结论:AciCC好发于女性,主要发病部位为大唾液腺。NR4A3基因重排仅见于AciCC中,在诊断工作中具有100%的特异性,但敏感性较低。NOR-1蛋白表达检测具有很好的灵敏度和特异度,可作为鉴别AciCC、MEC和SC的初筛和替代方法。联合使用NR4A3基因重排和NOR-1蛋白表达检测具有最优的诊断价值。 展开更多
关键词 腺泡细胞癌 唾液腺 NR4A3 基因重排 NOR-1 蛋白表达
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T细胞性淋巴瘤诊断中TCR基因重排检测的应用 被引量:7
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作者 潘鑫艳 杨长绍 +2 位作者 黎贵芸 杨举伦 王丽 《临床与实验病理学杂志》 CAS CSCD 北大核心 2015年第4期400-403,共4页
目的探讨T细胞受体(T cell receptors,TCR)基因重排检测对T细胞性淋巴瘤诊断的价值。方法收集T细胞性淋巴瘤30例和淋巴反应性增生组织30例,提取DNA,应用BIOMED-2引物系统中的56条引物进行PCR扩增,核酸分子异源双链凝胶电泳分析结果。结... 目的探讨T细胞受体(T cell receptors,TCR)基因重排检测对T细胞性淋巴瘤诊断的价值。方法收集T细胞性淋巴瘤30例和淋巴反应性增生组织30例,提取DNA,应用BIOMED-2引物系统中的56条引物进行PCR扩增,核酸分子异源双链凝胶电泳分析结果。结果 30例T细胞性淋巴瘤标本中TCRβ、TCRγ、TCRδ的检出率分别为83.3%(25/30)、93.3%(28/30)、13.3%(4/30),三者联合检测的检出率为96.7%(29/30),30例淋巴反应性增生组织中均未检测出TCR基因重排。结论利用BIOMED-2引物系统检测TCR基因重排可作为T细胞性淋巴瘤的辅助诊断工具。 展开更多
关键词 T细胞性淋巴瘤 基因重排 BIOMED-2 tcr基因
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