The presence of a relatively mature CD4 <sup>+</sup> CD8<sup>-</sup> （SP） T cell subset in mouse thymus has been demonstrated. Composing of 10% of total CD4SP thymocytes, this subset is defin...The presence of a relatively mature CD4 <sup>+</sup> CD8<sup>-</sup> （SP） T cell subset in mouse thymus has been demonstrated. Composing of 10% of total CD4SP thymocytes, this subset is defined by the absence of 3G11 and 6C10 expression with a phenotype of CD69<sup>+/-</sup>, HSA<sup>med/lo</sup> and heterogeneous for Qa-2 expression. The proliferation capability of TCRαβ<sup>+</sup> 3G11<sup>-</sup> 6C10<sup>-</sup> CD4<sup>+</sup> CD8<sup>-</sup> thymocytes was high while using Con A stimulus. And Con A stimulation could result in secretion of IL-4, IL-10, IL-6 and a little amount of IFNγ. IL-2 was barely detectable. This is distinct from typical Th0 type cytokines. The cells of this subset were NK1.1 negative, but strongly expressed GATA-3 mRNA. The results suggest that the CD4<sup>+</sup> subset of 3G11<sup>-</sup> 6C10<sup>-</sup> NK1.1<sup>-</sup> phenotype possesses immunocompetent cells with functions characteristic of Th2-like cytokines, which may indicate the cells at transitional status from Th0 to Th2, with a propensity to Th2.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant No. 39730410).
文摘The presence of a relatively mature CD4 <sup>+</sup> CD8<sup>-</sup> （SP） T cell subset in mouse thymus has been demonstrated. Composing of 10% of total CD4SP thymocytes, this subset is defined by the absence of 3G11 and 6C10 expression with a phenotype of CD69<sup>+/-</sup>, HSA<sup>med/lo</sup> and heterogeneous for Qa-2 expression. The proliferation capability of TCRαβ<sup>+</sup> 3G11<sup>-</sup> 6C10<sup>-</sup> CD4<sup>+</sup> CD8<sup>-</sup> thymocytes was high while using Con A stimulus. And Con A stimulation could result in secretion of IL-4, IL-10, IL-6 and a little amount of IFNγ. IL-2 was barely detectable. This is distinct from typical Th0 type cytokines. The cells of this subset were NK1.1 negative, but strongly expressed GATA-3 mRNA. The results suggest that the CD4<sup>+</sup> subset of 3G11<sup>-</sup> 6C10<sup>-</sup> NK1.1<sup>-</sup> phenotype possesses immunocompetent cells with functions characteristic of Th2-like cytokines, which may indicate the cells at transitional status from Th0 to Th2, with a propensity to Th2.
