Temporomandibular disorders (TMD) negatively affect quality of life, causing pain and restricted jaw movements. This study evaluates the effectiveness of Transcutaneous Electrical Nerve Stimulation (TENS) on TMD patie...Temporomandibular disorders (TMD) negatively affect quality of life, causing pain and restricted jaw movements. This study evaluates the effectiveness of Transcutaneous Electrical Nerve Stimulation (TENS) on TMD patients before orthodontic treatment at the Juarez Autonomous University of Tabasco. A quantitative, quasi-experimental study was conducted with 30 patients who met the inclusion criteria: aged 18 to 65 years, with TMJ pain symptoms, diagnosed with temporomandibular disorders using the Helkimo index, and who had provided informed consent for the study. The range of mandibular movement and the Visual Analog Scale (VAS) were measured to record values before therapy. The electrodes of the TENS therapy were positioned on both sides of the jaw in the preauricular area and the masseter muscle, the therapy was administered for 40 minutes, utilizing a frequency of 2 - 4 Hz and a pulse duration of 300 microseconds. Following this, the range of mandibular movement and the VAS were reassessed to evaluate the outcomes., significantly improved maximum mouth opening (37.7 ± 6.4 mm to 44.6 ± 5.88 mm), right laterality (3.86 ± 1.57 mm to 5.43 ± 1.21 mm), left laterality (3.13 ± 1.63 mm to 4.53 ± 1.61 mm), protrusion (4.01 ± 1.7 mm to 5.36 ± 1.32 mm), and pain perception (4.56 ± 2.045 to 2.03 ± 1.5) (P < 0.01). TENS therapy enhances mandibular movement and reduces pain, making it a well-tolerated, non-invasive complementary treatment for TMD.展开更多
目的观察二氢杨梅素对人乳腺癌MCF-7细胞中第10号染色体缺失的磷酸酶和张力蛋白同源基因(phosphatase and tensin homology deleted on chromosome ten,PTEN)甲基化及其表达的影响,并探讨相关作用机制。方法以CCK-8法检测细胞活力,qRT-...目的观察二氢杨梅素对人乳腺癌MCF-7细胞中第10号染色体缺失的磷酸酶和张力蛋白同源基因(phosphatase and tensin homology deleted on chromosome ten,PTEN)甲基化及其表达的影响,并探讨相关作用机制。方法以CCK-8法检测细胞活力,qRT-PCR检测PTEN和DNA甲基转移酶(DNA methyltransferase,DNMT)DNMT1、DNMT3a、DNMT3b的mRNA表达,Western blot法检测PTEN的蛋白表达,荧光法检测细胞DNA甲基转移酶总活性,甲基化特异性PCR法(methylation-specific PCR,MSP)检测PTEN基因甲基化水平。结果细胞活力检测结果表明,二氢杨梅素可剂量依赖性降低乳腺癌MCF-7细胞活力(P<0.05);qRT-PCR和Western blot检测结果表明,二氢杨梅素可显著上调PTEN的表达(P<0.05),并呈现剂量-效应关系;MSP检测结果显示,二氢杨梅素可显著诱导MCF-7细胞PTEN基因去甲基化;qRT-PCR和荧光法检测结果显示,二氢杨梅素可显著降低MCF-7细胞DNMT1表达和DNMT活性(P<0.05)。结论二氢杨梅素显著诱导乳腺癌细胞PTEN基因去甲基化,促进其表达,其机制可能主要涉及对DNMT1表达和活性的抑制。展开更多
人第10号染色体缺失的磷酸酶及张力蛋白同源(phosphatase and tensin homolog deleted on chromosome ten,PTEN)基因作为一种抑癌基因,在调控肿瘤方面的作用广为人知。近年来研究发现,PTEN除了在肿瘤防治上发挥作用外,还能通过抑制炎症...人第10号染色体缺失的磷酸酶及张力蛋白同源(phosphatase and tensin homolog deleted on chromosome ten,PTEN)基因作为一种抑癌基因,在调控肿瘤方面的作用广为人知。近年来研究发现,PTEN除了在肿瘤防治上发挥作用外,还能通过抑制炎症、氧化应激的发展来减缓动脉粥样硬化(atherosclerosis,AS)的进程。同时,PTEN蛋白能够抑制AS中血管平滑肌细胞(vascular smooth muscle cells,VSMCs)的异常增殖,以及调控巨噬细胞极化的方向、改变巨噬细胞表面清道夫受体的类型来抗AS。而PTEN对于血管内皮细胞(vascular endothelial cells,VECs)的调控则具有双重性。文章对PTEN的结构、功能与发挥的调控作用做一简要综述,以期为AS防治的基础研究提供新的思路和靶点。展开更多
文摘Temporomandibular disorders (TMD) negatively affect quality of life, causing pain and restricted jaw movements. This study evaluates the effectiveness of Transcutaneous Electrical Nerve Stimulation (TENS) on TMD patients before orthodontic treatment at the Juarez Autonomous University of Tabasco. A quantitative, quasi-experimental study was conducted with 30 patients who met the inclusion criteria: aged 18 to 65 years, with TMJ pain symptoms, diagnosed with temporomandibular disorders using the Helkimo index, and who had provided informed consent for the study. The range of mandibular movement and the Visual Analog Scale (VAS) were measured to record values before therapy. The electrodes of the TENS therapy were positioned on both sides of the jaw in the preauricular area and the masseter muscle, the therapy was administered for 40 minutes, utilizing a frequency of 2 - 4 Hz and a pulse duration of 300 microseconds. Following this, the range of mandibular movement and the VAS were reassessed to evaluate the outcomes., significantly improved maximum mouth opening (37.7 ± 6.4 mm to 44.6 ± 5.88 mm), right laterality (3.86 ± 1.57 mm to 5.43 ± 1.21 mm), left laterality (3.13 ± 1.63 mm to 4.53 ± 1.61 mm), protrusion (4.01 ± 1.7 mm to 5.36 ± 1.32 mm), and pain perception (4.56 ± 2.045 to 2.03 ± 1.5) (P < 0.01). TENS therapy enhances mandibular movement and reduces pain, making it a well-tolerated, non-invasive complementary treatment for TMD.
