用FT—IR技术研究人卵泡促性腺激素释放肽hF—GRP及其类似物TF14的二级结构

用付立叶变换红外（ＦＴ—ＩＲ）技术，定量分析了人卵泡促性腺激素释放肽ｈＦ—ＧＲＰ及其类似物ＴＦ１４这两个多肽在重水溶液中的二级结构，结果为ｈＦ－ＧＲＰ有近四分之一的残基处于无规卷曲，其余部分是非典型的伸展结构，ＴＦ１４则基本上处于伸展和转角结构，无规和螺旋的成分很少，这与用二维核磁共振技术测定的ｈＦ—ＧＲＰ和ＴＦ１４的溶液构象实验结果非常吻合。

用~1H NMR和FT-IR研究hF-GRP及其类似物TF14与DMPC脂质体的相互作用

用１ＨＮＭＲ和ＦＴ－ＩＲ技术研究了肽激素ｈＦ－ＧＲＰ及其类似物ＴＦ１４与ＤＭＰＣ脂质体的相互作用及其构象变化，１Ｈ－ＮＭＲ实验结果提示这两个肽与ＤＭＰＣ之间存在静电相互作用，这使其可从水相结合到脂相中。ＦＴ－ＩＲ实验结果显示它们的构象从水相到脂相确实发生了某些调整变化，ｈＦ－ＧＲＰ趋于更加固定和弯曲，ＴＦ１４趋于更加伸展。以上工作为进一步从事ｈＦ－ＧＲＰ和ＴＦ１４受体结合的研究奠定了基础。

用2-D-NMR测定人卵泡促性腺激素释放肽类似物TF14的溶液构象

人卵泡促性腺激素释放肽(hF-GRP)被发现以后,人们合成了许多类似物以便寻找其激动剂或抑制剂,类似物TF14的活性就比hF-GRP强近1倍,为了探求结构活性关系,用2-D-NMR技术(TOCSY,ROESY)测定了TF14的溶液构象,得到了与hF-GRP有显著不同的结果,该14肽整个处于1个非典型的伸展结构.

Conformation of an analog of human follicular gonadotropin releasing peptide TF14 in solution by 2D-NMR

Since the discovery of hF-GRP, several analogs have been synthesized in order to see their effects on the gonadotropin releasing activity, either as agonists or antagonists to this peptide. TF14 is one of these analogs, whose 14th position in the primary sequence is Phe instead of Asn in hF-GRP, while its activity is doubled. 2D-NMR (TOCSY, ROESY) was used to determine the conformation of TF14 in solution. Compared with hF-GRP, the whole peptide is in a non-typical more extended conformation, which may give some due to the relation between structure and function of these two peptides.