CD8^(+)T cells play a central role in antiviral immune responses.Upon infection,naive CD8^(+)T cells differentiate into effector cells to eliminate virus-infected cells,and some of these effector cells further differe...CD8^(+)T cells play a central role in antiviral immune responses.Upon infection,naive CD8^(+)T cells differentiate into effector cells to eliminate virus-infected cells,and some of these effector cells further differentiate into memory cells to provide long-term protection after infection is resolved.Although extensively investigated,the underlying mechanisms of CD+T-cell differentiation remain incompletely understood.Themis is a T-cell-specific protein that plays critical roles in T-cell development.Recent studies using Themis T-cell conditional knockout mice also demonstrated that Themis is required to promote mature CD8^(+)T-cell homeostasis,cytokine responsiveness,and antibacterial responses.In this study,we used LCMV Armstrong infection as a probe to explore the role of Themis in viral infection.We found that preexisting CD8^(+)T-cell homeostasis defects and cytokine hyporesponsiveness do not impair viral clearance in Themis T-cell conditional knockout mice.Further analyses showed that in the primary immune response,Themis deficiency promoted the differentiation of CD8^(+)effector cells and increased their TNF and IFNy production.Moreover,Themis deficiency impaired memory precursor cell(MPEC)differentiation but promoted short-lived effector cell(SLEC)differentiation.Themis deficiency also enhanced effector cytokine production in memory CD8^(+)T cells while impairing central memory CD8^(+)T-cell formation.Mechanistically,we found that Themis mediates PD-1 expression and its signaling in effector CD8^(+)T cells,which explains the elevated cytokine production in these cells when Themis is disrupted.展开更多
In the past two years, many progresses have been made in magnetospheric physics by using the data of Double Star Program, Cluster, THEMIS and RBSP missions, or by computer simulations. This paper briefly reviews these...In the past two years, many progresses have been made in magnetospheric physics by using the data of Double Star Program, Cluster, THEMIS and RBSP missions, or by computer simulations. This paper briefly reviews these works based on papers selected from the 126 publications from March 2012 to March 2014. The subjects cover various sub-branches of magnetospheric physics,including geomagnetic storm, magnetospheric substorm and magnetic reconnection.展开更多
The cause of substorm onset is not yet understood. Chen CX(2016) proposed an entropy switch model, in which substorm onset results from the development of interchange instability. In this study, we sought observationa...The cause of substorm onset is not yet understood. Chen CX(2016) proposed an entropy switch model, in which substorm onset results from the development of interchange instability. In this study, we sought observational evidence for this model by using Time History of Events and Macroscale Interactions during Substorms(THEMIS) data. We examined two events, one with and the other without a streamer before substorm onset. In contrast to the stable magnetosphere, where the total magnetic field strength is a decreasing function and entropy is an increasing function of the downtail distance, in both events the total magnetic field strength and entropy were reversed before substorm onset. After onset, the total magnetic field strength, entropy, and other plasma quantities fluctuated. In addition, a statistical study was performed. By confining the events with THEMIS satellites located in the downtail region between ~8 and ~12 Earth radii, and 3 hours before and after midnight, we found the occurrence rate of the total magnetic field strength reversal to be 69% and the occurrence rate of entropy reversal to be 77% of the total 205 events.展开更多
基金the Core Facility of Biomedical Sciences,Xiamen University for Flow Cytometry Cell Sorting service.This study was supported by the National Natural Science Foundation of China(32070887)the Fundamental Research Funds for the Central Universities(20720220003)to G.F.Singapore Ministry of Education(MOE-000112)Singapore Ministry of Health's National Medical Research Council(MOH-000523)to N.RJ.Gascoigne.The Natural Science Foundation of Fujian Province(No.2019J01009)to Q.L.The National Natural Science Foundation of China(U1904206)to X.L.C.The funders had no role in the study design,data collection and analysis,decision to publish,or preparation of the manuscript。
文摘CD8^(+)T cells play a central role in antiviral immune responses.Upon infection,naive CD8^(+)T cells differentiate into effector cells to eliminate virus-infected cells,and some of these effector cells further differentiate into memory cells to provide long-term protection after infection is resolved.Although extensively investigated,the underlying mechanisms of CD+T-cell differentiation remain incompletely understood.Themis is a T-cell-specific protein that plays critical roles in T-cell development.Recent studies using Themis T-cell conditional knockout mice also demonstrated that Themis is required to promote mature CD8^(+)T-cell homeostasis,cytokine responsiveness,and antibacterial responses.In this study,we used LCMV Armstrong infection as a probe to explore the role of Themis in viral infection.We found that preexisting CD8^(+)T-cell homeostasis defects and cytokine hyporesponsiveness do not impair viral clearance in Themis T-cell conditional knockout mice.Further analyses showed that in the primary immune response,Themis deficiency promoted the differentiation of CD8^(+)effector cells and increased their TNF and IFNy production.Moreover,Themis deficiency impaired memory precursor cell(MPEC)differentiation but promoted short-lived effector cell(SLEC)differentiation.Themis deficiency also enhanced effector cytokine production in memory CD8^(+)T cells while impairing central memory CD8^(+)T-cell formation.Mechanistically,we found that Themis mediates PD-1 expression and its signaling in effector CD8^(+)T cells,which explains the elevated cytokine production in these cells when Themis is disrupted.
文摘In the past two years, many progresses have been made in magnetospheric physics by using the data of Double Star Program, Cluster, THEMIS and RBSP missions, or by computer simulations. This paper briefly reviews these works based on papers selected from the 126 publications from March 2012 to March 2014. The subjects cover various sub-branches of magnetospheric physics,including geomagnetic storm, magnetospheric substorm and magnetic reconnection.
基金supported by the National Natural Science Foundation of China(Grant No.NSFC41974204)。
文摘The cause of substorm onset is not yet understood. Chen CX(2016) proposed an entropy switch model, in which substorm onset results from the development of interchange instability. In this study, we sought observational evidence for this model by using Time History of Events and Macroscale Interactions during Substorms(THEMIS) data. We examined two events, one with and the other without a streamer before substorm onset. In contrast to the stable magnetosphere, where the total magnetic field strength is a decreasing function and entropy is an increasing function of the downtail distance, in both events the total magnetic field strength and entropy were reversed before substorm onset. After onset, the total magnetic field strength, entropy, and other plasma quantities fluctuated. In addition, a statistical study was performed. By confining the events with THEMIS satellites located in the downtail region between ~8 and ~12 Earth radii, and 3 hours before and after midnight, we found the occurrence rate of the total magnetic field strength reversal to be 69% and the occurrence rate of entropy reversal to be 77% of the total 205 events.
