In Beyond the Fifth Generation(B5G)heterogeneous edge networks,numerous users are multiplexed on a channel or served on the same frequency resource block,in which case the transmitter applies coding and the receiver u...In Beyond the Fifth Generation(B5G)heterogeneous edge networks,numerous users are multiplexed on a channel or served on the same frequency resource block,in which case the transmitter applies coding and the receiver uses interference cancellation.Unfortunately,uncoordinated radio resource allocation can reduce system throughput and lead to user inequity,for this reason,in this paper,channel allocation and power allocation problems are formulated to maximize the system sum rate and minimum user achievable rate.Since the construction model is non-convex and the response variables are high-dimensional,a distributed Deep Reinforcement Learning(DRL)framework called distributed Proximal Policy Optimization(PPO)is proposed to allocate or assign resources.Specifically,several simulated agents are trained in a heterogeneous environment to find robust behaviors that perform well in channel assignment and power allocation.Moreover,agents in the collection stage slow down,which hinders the learning of other agents.Therefore,a preemption strategy is further proposed in this paper to optimize the distributed PPO,form DP-PPO and successfully mitigate the straggler problem.The experimental results show that our mechanism named DP-PPO improves the performance over other DRL methods.展开更多
The simultaneous wireless information and power transfer(SWIPT)relay system is one of the emerging technologies.Xiaomi Corporation and Motorola Inc.recently launched indoor wireless power transfer equipment is one of ...The simultaneous wireless information and power transfer(SWIPT)relay system is one of the emerging technologies.Xiaomi Corporation and Motorola Inc.recently launched indoor wireless power transfer equipment is one of the most promising applications.To tap the potential of the system,hybrid automatic repeat request(HARQ)is introduced into the SWIPT relay system.Firstly,the time slot structure of HARQ scheme based on full duplex two-way amplify and forward(AF)SWIPT relay is given,and its retransmission status is analyzed.Secondly,the equivalent signal-to-noise ratio and outage probability of various states are calculated by approximate simplification.Thirdly,the energy harvesting power in each state is calculated.Finally,the energy harvested-throughput sum function is constructed to characterize the performance of energy harvesting and data transmission.Simulation results show that the proposed HARQ scheme has better energy harvestedthroughput sum function than the traditional HARQ scheme.When P_(2)=22 dB,the maximum sum function is 54.86%(the proposed HARQ scheme)and 52.307%(the traditional HARQ scheme),respectively.展开更多
The minimum energy per bit(EPB)as the energy efficiency(EE)metric in an automatic retransmission request(ARQ)based multi-hop system is analyzed under power and throughput constraints.Two ARQ protocols including type-I...The minimum energy per bit(EPB)as the energy efficiency(EE)metric in an automatic retransmission request(ARQ)based multi-hop system is analyzed under power and throughput constraints.Two ARQ protocols including type-I(ARQ-I)and repetition redundancy(ARQ-RR)are considered and expressions for the optimal power allocation(PA)are obtained.Using the obtained optimal powers,the EE-throughput tradeoff(EETT)is analyzed and the EETT closed-form expressions for both ARQ protocols and in arbitrary average channel gain values are obtained.It is shown that how different throughput requirements,especially the high levels,affect the EE performance.Additionally,asymptotic analysis is made in the feasible high throughput values and lower and upper EETT bounds are derived for ARQ-I protocol.To evaluate the EE a distributed PA scenario,as a benchmark,is presented and the energy savinggain obtained from the optimal PA in comparison with the distributed PA for ARQ-I and ARQ-RR protocols is discussed in different throughput values and node locations.展开更多
Single atomic catalysts(SACs),especially metal-nitrogen doped carbon(M-NC)catalysts,have been extensively explored for the electrochemical oxygen reduction reaction(ORR),owing to their high activity and atomic utiliza...Single atomic catalysts(SACs),especially metal-nitrogen doped carbon(M-NC)catalysts,have been extensively explored for the electrochemical oxygen reduction reaction(ORR),owing to their high activity and atomic utilization efficiency.However,there is still a lack of systematic screening and optimization of local structures surrounding active centers of SACs for ORR as the local coordination has an essential impact on their electronic structures and catalytic performance.Herein,we systematic study the ORR catalytic performance of M-NC SACs with different central metals and environmental atoms in the first and second coordination sphere by using density functional theory(DFT)calculation and machine learning(ML).The geometric and electronic informed overpotential model(GEIOM)based on random forest algorithm showed the highest accuracy,and its R^(2) and root mean square errors(RMSE)were 0.96 and 0.21,respectively.30 potential high-performance catalysts were screened out by GEIOM,and the RMSE of the predicted result was only 0.12 V.This work not only helps us fast screen high-performance catalysts,but also provides a low-cost way to improve the accuracy of ML models.展开更多
In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection...In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection results,the laboratory information management system was used as the information platform to design a high-throughput laboratory automation pre-treatment system based on the deep integration of mechanical principles,visual analysis,high-speed conduction,intelligent storage and other technical systems.The experimental results showed that the system could shorten the sample circulation cycle,effectively improve the laboratory biosafety,and meet the requirements of high-throughput processing of samples.展开更多
Chemical vapor deposition is considered as the most hopeful method for the synthesis of large-area high-quality hexagonal boron nitride on the substrate of catalytic metal. However, the size the hexagonal boron nitrid...Chemical vapor deposition is considered as the most hopeful method for the synthesis of large-area high-quality hexagonal boron nitride on the substrate of catalytic metal. However, the size the hexagonal boron nitride films are limited to the size of growth chamber, which indicates a lower production efficiency. In this paper, the utilization efficiency of growth chamber is highly improved by alternately stacking multiple pieces of Cu foils and carbon fiber surface felt with porous structure. Uniform and continuous hexagonal boron nitride films are prepared on Cu foils through chemical vapor deposition utilizing ammonia borane as the precursor. This work develops a simple and practicable method for high-throughput preparation of hexagonal boron nitride films, which could contribute to the industrial application of hexagonal boron nitride. .展开更多
This paper proposes a raptor-like low-density parity-check(RL-LDPC)code design together with the corresponding decoder hardware architecture aiming at next-generation mobile communication.A new kind of protograph diff...This paper proposes a raptor-like low-density parity-check(RL-LDPC)code design together with the corresponding decoder hardware architecture aiming at next-generation mobile communication.A new kind of protograph different from the 5G new radio(NR)LDPC basic matrix is presented,and a code construction algorithm is proposed to improve the error-correcting performance.A multi-core layered decoder architecture that supports up to 100 Gbit/s throughput is designed based on the special protograph structure.展开更多
As an essential component of the Materials Genome Initiative aiming to shorten the period of materials research and development, combinatorial synthesis and rapid characterization technologies have been playing a more...As an essential component of the Materials Genome Initiative aiming to shorten the period of materials research and development, combinatorial synthesis and rapid characterization technologies have been playing a more and more important role in exploring new materials and comprehensively understanding materials properties. In this review, we discuss the advantages of high-throughput experimental techniques in researches on superconductors. The evolution of combinatorial thin-film technology and several high-speed screening devices are briefly introduced. We emphasize the necessity to develop new high-throughput research modes such as a combination of high-throughput techniques and conventional methods.展开更多
The experiments of high throughput drilling of Ti-6Al-4V at 183 m/min cutting speed and 156 mm^3/s material removal rate using a 4 mm diameter WC-Co spiral point drill are conducted. At this material removal rate, it ...The experiments of high throughput drilling of Ti-6Al-4V at 183 m/min cutting speed and 156 mm^3/s material removal rate using a 4 mm diameter WC-Co spiral point drill are conducted. At this material removal rate, it took only 0.57 s to drill a hole in a 6.35 mm thick Ti plate. Supplying the cutting fluid via through-the-drill holes and the balance of cutting speed and feed have proven to be critical for drill life. An inverse heat transfer model is developed to predict the heat flux and the drill temperature distribution in drilling. A three-dimensional finite element modeling of drilling is con-ducted to predict the thrust force and torque. Experimental result demonstrates that, using proper machining process parameters, tool geometry, and fine-grained WC-Co tool material, the high throughput machining of Ti alloy is technically feasible.展开更多
RNA sequencing is the use of hight hroughput next generation sequencing technology to survey, characterize, and quantify the transcriptome of a genome. RNA sequencing has been used to analyze the pathogenesis of sever...RNA sequencing is the use of hight hroughput next generation sequencing technology to survey, characterize, and quantify the transcriptome of a genome. RNA sequencing has been used to analyze the pathogenesis of several malignancies such melanoma, lung cancer, and colorectal cancer. RNA sequencing can identify differential expression of genes(DEG's), mutated genes, fusion genes, and gene isoforms in disease states. RNA sequencing has been used in the investigation of several colorectal diseases such as colorectal cancer, inflammatory bowel disease(ulcerative colitis and Crohn's disease), and irritable bowel syndrome.展开更多
This paper presents a performance study of the distributed coordination function (DCF) of 802.11 networks considering erroneous channel and capture effects under non-saturated traffic conditions employing a basic ac...This paper presents a performance study of the distributed coordination function (DCF) of 802.11 networks considering erroneous channel and capture effects under non-saturated traffic conditions employing a basic access method.The aggregate throughput of a practical wireless local area network (WLAN) strongly depends on the channel conditions.In a real radio environment,the received signal power at the access point from a station is subjected to deterministic path loss,shadowing,and fast multipath fading.The binary exponential backoff (BEB) mechanism of IEEE 802.11 DCF severely suffers from more channel idle time under high bit error rate (BER).To alleviate the low performance of IEEE 802.11 DCF,a new mechanism is introduced,which greatly outperforms the existing methods under a high BER.A multidimensional Markov chain model is used to characterize the behavior of DCF in order to account both non-ideal channel conditions and capture effects.展开更多
Our aim is to study the roles of a new emerging group of non-coding RNAs, circRNAs, in tomato(Solanum lycopersicum L.) plants grown at the combination of drought and heat, two of the most usual stress conditions known...Our aim is to study the roles of a new emerging group of non-coding RNAs, circRNAs, in tomato(Solanum lycopersicum L.) plants grown at the combination of drought and heat, two of the most usual stress conditions known to frequently happen in field. Tomato seedlings from cultivar‘Jinling Meiyu’ were treated without stresses(control), at water shortage, high temperature and subjected the multiple stresses. In total, 467 circRNAs were identified with 87.82% from exon using high throughput sequencing technology. Among the circRNAs, 70 were from chr1 with the range from 23 to 49 from the other chromosomes. In detail, 156 circRNAs were shared in the four libraries, while 21, 17 and 36 circRNAs were only shown in drought, heat and multiple stresses libraries, respectively. Through a differential expression analysis, four, seven and nine circRNAs were differentially regulated in tomato at drought, heat and multiple stresses as compared with control. These circRNAs played roles on photosynthesis, starch and sucrose metabolism, RNA transport, RNA degradation, spliceosome, ribosome, etc. Our study underlined the potential role of circRNAs involved in the abiotic stress response in tomato, which might pave the way for studying biological roles of circRNAs responding to multiple stresses in plants.展开更多
In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park,the ITS+5.8S rDNA gene and 16S rDNA...In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park,the ITS+5.8S rDNA gene and 16S rDNA gene V3-V4 region sequencing analysis for fungi and bacteria,respectively,were conducted by high throughput sequencing(Illumina MiSeq).The results showed that the fungal diversity in the rhizosphere soil samples of C.coggygria in Fragrant Hills park in 2018 was significantly higher than that in 2016,and it was higher in the rhizosphere soil of healthy C.coggygria in Xunlupo than that in diseased ones in 2018.Verticillium dahliae,which is the causal agent of C.coggygria wilt,was detected in five soil samples.In 2018,the bacterial diversity in the rhizosphere soil of diseased C.coggygria in Xunlupo was the lowest,while it was the highest in the rhizosphere soil of healthy C.coggygria under Platycladus orientalis in Langfengting.展开更多
This study was conducted to investigate the phylogenetic diversity of archaea in the rumen of adult and elderly yaks. Six domesticated female yaks, 3 adult yaks ((5.3±0.6) years old), and 3 elderly yaks ((1...This study was conducted to investigate the phylogenetic diversity of archaea in the rumen of adult and elderly yaks. Six domesticated female yaks, 3 adult yaks ((5.3±0.6) years old), and 3 elderly yaks ((10.7±0.6) years old), were used for the rumen contents collection. Illumina MiSeq high-throughput sequencing technology was applied to examine the archaeal composition of rumen contents. A total of 92 901 high-quality archaeal sequences were analyzed, and these were assigned to 2 033 operational taxonomic units (OTUs). Among these, 974 OTUs were unique to adult yaks while 846 OTUs were unique to elderly yaks; 213 OTUs were shared by both groups. At the phylum level, more than 99% of the obtained OTUs belonged to the Euryarchaeota phylum. At the genus level, the archaea could be divided into 7 archaeal genera. The 7 genera (i.e., Methanobrevibacter, Methanobacterium, Methanosphaera, Thermogymnomonas, Methanomicrobiu, Meth- animicrococcus and the unclassified genus) were shared by all yaks, and their total abundance accounted for 99% of the rumen archaea. The most abundant archaea in elderly and adult yaks were Methanobrevibacterand Thermogymnomonas, respectively. The abundance of Methanobacteria (class), Methanobacteriales (order), Methanobacteriaceae (family), and Methanobrevibacter (genus) in elderly yaks was significantly higher than in adult yaks. In contrast, the abundance of Ther-mogymnomonas in elderly yaks was 34% lower than in adult yaks, though the difference was not statistically significant. The difference in abundance of other archaea was not significant between the two groups. These results suggested that the structure of archaea in the rumen of yaks changed with age. This is the first study to compare the phytogenetic differences of rumen archaeal structure and composition using the yak model.展开更多
Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has...Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still limited.Results: Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver,muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine(A-to-G) conversion. Most A-to-G sites were located in noncoding regions and generally had low editing levels. In total, 151 A-to-G sites were detected in coding regions(CDS), including 94 sites that could lead to nonsynonymous amino acid changes. We provide further evidence supporting a previous observation that pig transcriptomes are highly editable at PRE-1 elements. The number of A-to-G editing sites ranged from 4155(muscle) to 25001(brain) across the seven tissues. The expression levels of the ADAR enzymes could explain some but not all of this variation across tissues. The functional analysis of the genes with tissuespecific editing sites in each tissue revealed that RNA editing might play important roles in tissue function.Specifically, more pathways showed significant enrichment in the fat and liver than in other tissues, while no pathway was enriched in the muscle.Conclusions: This study identified a total of 74863 nonredundant RNA editing sites in seven tissues and revealed the potential importance of RNA editing in tissue function. Our findings largely extend the porcine editome and enhance our understanding of RNA editing in swine.展开更多
Background:In-feed antibiotics are being phased out in livestock production worldwide.Alternatives to antibiotics are urgently needed to maintain animal health and production performance.Host defense peptides(HDPs)are...Background:In-feed antibiotics are being phased out in livestock production worldwide.Alternatives to antibiotics are urgently needed to maintain animal health and production performance.Host defense peptides(HDPs)are known for their broad-spectrum antimicrobial and immunomodulatory capabilities.Enhancing the synthesis of endogenous HDPs represents a promising antibiotic alternative strategy to disease control and prevention.Methods:To identify natural products with an ability to stimulate the synthesis of endogenous HDPs,we performed a high-throughput screening of 1261 natural products using a newly-established stable luciferase reporter cell line known as IPEC-J2/pBD3-luc.The ability of the hit compounds to induce HDP genes in porcine IPEC-J2 intestinal epithelial cells,3D4/31 macrophages,and jejunal explants were verified using RT-qPCR.Augmentation of the antibacterial activity of porcine 3D4/31 macrophages against a Gram-negative bacterium(enterotoxigenic E.coli)and a Gram-positive bacterium(Staphylococcus aureus)were further confirmed with four selected HDP-inducing compounds.Results:A total of 48 natural products with a minimum Z-score of 2.0 were identified after high-throughput screening,with 21 compounds giving at least 2-fold increase in luciferase activity in a follow-up dose-response experiment.Xanthohumol and deoxyshikonin were further found to be the most potent in inducing pBD3 mRNA expression,showing a minimum 10-fold increase in IPEC-J2,3D4/31 cells,and jejunal explants.Other compounds such as isorhapontigenin and calycosin also enhanced pBD3 mRNA expression by at least 10-fold in both IPEC-J2 cells and jejunal explants,but not 3D4/31 cells.In addition to pBD3,other porcine HDP genes such as pBD2,PG1-5,and pEP2C were induced to different magnitudes by xanthohumol,deoxyshikonin,isorhapontigenin,and calycosin,although clear gene-and cell type-specific patterns of regulation were observed.Desirably,these four compounds had a minimum effect on the expression of several representative inflammatory cytokine genes.Furthermore,when used at HDP-inducing concentrations,these compounds showed no obvious direct antibacterial activity,but significantly augmented the antibacterial activity of 3D4/31 macrophages(P<0.05)against both Gram-negative and Gram-positive bacteria.Conclusions:Our results indicate that these newly-identified natural HDP-inducing compounds have the potential to be developed as novel alternatives to antibiotics for prophylactic and therapeutic treatment of infectious diseases in livestock production.展开更多
Huguangyan Maar Lake is a typical maar lake in the southeast of China. It is well preserved and not disturbed by anthropogenic activities. In this study, microbial community structures in sediment and water samples fr...Huguangyan Maar Lake is a typical maar lake in the southeast of China. It is well preserved and not disturbed by anthropogenic activities. In this study, microbial community structures in sediment and water samples from Huguangyan Maar Lake were investigated using a high-throughput sequencing method. We found significant differences between the microbial community compositions of the water and the sediment. The sediment samples contained more diverse Bacteria and Archaea than did the water samples. Actinobacteria, Betaproteobacteria, Cyanobacteria, and Deltaproteobacteria predominated in the water samples while Deltaproteobacteria, Anaerolineae, Nitrospira, and Dehalococcoidia were the major bacterial groups in the sediment. As for Archaea, Woesearchaeota (DHVEG-6), unclassified Archaea, and Deep Sea Euryarchaeotic Group were detected at higher abundances in the water, whereas the Miscellaneous Crenarchaeotic Group, Thermoplasmata, and Methanomicrobia were significantly more abundant in the sediment. Interactions between Bacteria and Archaea were common in both the water column and the sediment. The concentrations of major nutrients (NO^3-, PO4^3-, SiO3^2- and NH4^+) shaped the microbial population structures in the water. At the higher phylogenetic levels including phylum and class, many of the dominant groups were those that were also abundant in other lakes;however, novel microbial populations (unclassified) were often seen at the lower phylogenetic levels. Our study lays a foundation for examining microbial biogeochemical cycling in sequestered lakes or reservoirs.展开更多
Gene sequencing is a great way to interpret life, and high-throughput sequencing technology is a revolutionary technological innovation in gene sequencing researches. This technology is characterized by low cost and h...Gene sequencing is a great way to interpret life, and high-throughput sequencing technology is a revolutionary technological innovation in gene sequencing researches. This technology is characterized by low cost and high-throughput data. Currently, high-throughput sequencing technology has been widely applied in multi-level researches on genomics, transcriptomics and epigenomics. And it has fundamentally changed the way we approach problems in basic and translational researches and created many new possibilities. This paper presented a general description of high-throughput sequencing technology and a comprehensive review of its application with plain, concisely and precisely. In order to help researchers finish their work faster and better, promote science amateurs and understand it easier and better.展开更多
The exponentially increasing number of heterogeneous Internet of Things(Io T)devices(e.g.,Wi Fi and Zig Bee)crowed in the same ISM band(2.4 G)and recent advances in CrossTechnology Communications(CTC)motivate us to ex...The exponentially increasing number of heterogeneous Internet of Things(Io T)devices(e.g.,Wi Fi and Zig Bee)crowed in the same ISM band(2.4 G)and recent advances in CrossTechnology Communications(CTC)motivate us to explore more efficient data collection and maximize network throughput.CTC enables Wi Fi and Zig Bee devices to communicate directly without any hardware changes or gateway equipment,which sheds light on a more efficient data collection design.In this work,we propose a distributed algorithm,named Max Bee,to compute the maximum network throughput,which is formulated as a linear programming problem.Considering that the problem turns out to be non-convex and hard to solve exactly,we propose a distributed algorithm to solve nonlinear programming by using the dual decomposition method and gradient/subgradient algorithms.Through extensive simulations on different sets of deployed Zig Bee and Wi Fi devices,we observe that the proposed algorithm significantly increases the network throughput based on CTC for Sensor Networks.展开更多
This paper investigates the power control and routing problem in the communication process of an energy harvesting(EH)multi-hop cognitive radio network(CRN).The secondary user(SU)nodes(i.e.,source node and relay nodes...This paper investigates the power control and routing problem in the communication process of an energy harvesting(EH)multi-hop cognitive radio network(CRN).The secondary user(SU)nodes(i.e.,source node and relay nodes)harvest energy from the environment and use the energy exclusively for transmitting data.The SU nodes(i.e.,relay nodes)on the path,store and forward the received data to the destination node.We consider a real world scenario where the EH-SU node has only local causal knowledge,i.e.,at any time,each EH-SU node only has knowledge of its own EH process,channel state and currently received data.In order to study the power and routing issues,an optimization problem that maximizes path throughput considering quality of service(QoS)and available energy constraints is proposed.To solve this optimization problem,we propose a hybrid game theory routing and power control algorithm(HGRPC).The EH-SU nodes on the same path cooperate with each other,but EH-SU nodes on the different paths compete with each other.By selecting the best next hop node,we find the best strategy that can maximize throughput.In addition,we have established four steps to achieve routing,i.e.,route discovery,route selection,route reply,and route maintenance.Compared with the direct transmission,HGRPC has advantages in longer distances and higher hop counts.The algorithm generates more energy,reduces energy consumption and increases predictable residual energy.In particular,the time complexity of HGRPC is analyzed and its convergence is proved.In simulation experiments,the performance(i.e.,throughput and bit error rate(BER))of HGRPC is evaluated.Finally,experimental results show that HGRPC has higher throughput,longer network life,less latency,and lower energy consumption.展开更多
基金supported by the Key Research and Development Program of China(No.2022YFC3005401)Key Research and Development Program of China,Yunnan Province(No.202203AA080009,202202AF080003)Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX21_0482).
文摘In Beyond the Fifth Generation(B5G)heterogeneous edge networks,numerous users are multiplexed on a channel or served on the same frequency resource block,in which case the transmitter applies coding and the receiver uses interference cancellation.Unfortunately,uncoordinated radio resource allocation can reduce system throughput and lead to user inequity,for this reason,in this paper,channel allocation and power allocation problems are formulated to maximize the system sum rate and minimum user achievable rate.Since the construction model is non-convex and the response variables are high-dimensional,a distributed Deep Reinforcement Learning(DRL)framework called distributed Proximal Policy Optimization(PPO)is proposed to allocate or assign resources.Specifically,several simulated agents are trained in a heterogeneous environment to find robust behaviors that perform well in channel assignment and power allocation.Moreover,agents in the collection stage slow down,which hinders the learning of other agents.Therefore,a preemption strategy is further proposed in this paper to optimize the distributed PPO,form DP-PPO and successfully mitigate the straggler problem.The experimental results show that our mechanism named DP-PPO improves the performance over other DRL methods.
基金This work was supported by the National Natural Science Foundation of China(Grants No.61701251,62071244,62071249,61872423,61801236 and 61806100)Open Fund of Key Laboratory of Icing and Anti/De-icing(Grant No.IADL20190105)the Natural Science Foundation of Jiangsu Province(Grants No.BK20160903).
文摘The simultaneous wireless information and power transfer(SWIPT)relay system is one of the emerging technologies.Xiaomi Corporation and Motorola Inc.recently launched indoor wireless power transfer equipment is one of the most promising applications.To tap the potential of the system,hybrid automatic repeat request(HARQ)is introduced into the SWIPT relay system.Firstly,the time slot structure of HARQ scheme based on full duplex two-way amplify and forward(AF)SWIPT relay is given,and its retransmission status is analyzed.Secondly,the equivalent signal-to-noise ratio and outage probability of various states are calculated by approximate simplification.Thirdly,the energy harvesting power in each state is calculated.Finally,the energy harvested-throughput sum function is constructed to characterize the performance of energy harvesting and data transmission.Simulation results show that the proposed HARQ scheme has better energy harvestedthroughput sum function than the traditional HARQ scheme.When P_(2)=22 dB,the maximum sum function is 54.86%(the proposed HARQ scheme)and 52.307%(the traditional HARQ scheme),respectively.
文摘The minimum energy per bit(EPB)as the energy efficiency(EE)metric in an automatic retransmission request(ARQ)based multi-hop system is analyzed under power and throughput constraints.Two ARQ protocols including type-I(ARQ-I)and repetition redundancy(ARQ-RR)are considered and expressions for the optimal power allocation(PA)are obtained.Using the obtained optimal powers,the EE-throughput tradeoff(EETT)is analyzed and the EETT closed-form expressions for both ARQ protocols and in arbitrary average channel gain values are obtained.It is shown that how different throughput requirements,especially the high levels,affect the EE performance.Additionally,asymptotic analysis is made in the feasible high throughput values and lower and upper EETT bounds are derived for ARQ-I protocol.To evaluate the EE a distributed PA scenario,as a benchmark,is presented and the energy savinggain obtained from the optimal PA in comparison with the distributed PA for ARQ-I and ARQ-RR protocols is discussed in different throughput values and node locations.
基金financially supported by the National Key Research and Development Program of China (2018YFA0702002)the Beijing Natural Science Foundation (Z210016)the National Natural Science Foundation of China (21935001)。
文摘Single atomic catalysts(SACs),especially metal-nitrogen doped carbon(M-NC)catalysts,have been extensively explored for the electrochemical oxygen reduction reaction(ORR),owing to their high activity and atomic utilization efficiency.However,there is still a lack of systematic screening and optimization of local structures surrounding active centers of SACs for ORR as the local coordination has an essential impact on their electronic structures and catalytic performance.Herein,we systematic study the ORR catalytic performance of M-NC SACs with different central metals and environmental atoms in the first and second coordination sphere by using density functional theory(DFT)calculation and machine learning(ML).The geometric and electronic informed overpotential model(GEIOM)based on random forest algorithm showed the highest accuracy,and its R^(2) and root mean square errors(RMSE)were 0.96 and 0.21,respectively.30 potential high-performance catalysts were screened out by GEIOM,and the RMSE of the predicted result was only 0.12 V.This work not only helps us fast screen high-performance catalysts,but also provides a low-cost way to improve the accuracy of ML models.
文摘In the process of food testing,human operation is an important variable affecting the experimental results.In order to reasonably avoid the influence of human subjective operation behavior on the accuracy of detection results,the laboratory information management system was used as the information platform to design a high-throughput laboratory automation pre-treatment system based on the deep integration of mechanical principles,visual analysis,high-speed conduction,intelligent storage and other technical systems.The experimental results showed that the system could shorten the sample circulation cycle,effectively improve the laboratory biosafety,and meet the requirements of high-throughput processing of samples.
文摘Chemical vapor deposition is considered as the most hopeful method for the synthesis of large-area high-quality hexagonal boron nitride on the substrate of catalytic metal. However, the size the hexagonal boron nitride films are limited to the size of growth chamber, which indicates a lower production efficiency. In this paper, the utilization efficiency of growth chamber is highly improved by alternately stacking multiple pieces of Cu foils and carbon fiber surface felt with porous structure. Uniform and continuous hexagonal boron nitride films are prepared on Cu foils through chemical vapor deposition utilizing ammonia borane as the precursor. This work develops a simple and practicable method for high-throughput preparation of hexagonal boron nitride films, which could contribute to the industrial application of hexagonal boron nitride. .
基金supported in part by ZTE Industry-University-Institute Coop⁃eration funds under Grant No.2020ZTE01-03.
文摘This paper proposes a raptor-like low-density parity-check(RL-LDPC)code design together with the corresponding decoder hardware architecture aiming at next-generation mobile communication.A new kind of protograph different from the 5G new radio(NR)LDPC basic matrix is presented,and a code construction algorithm is proposed to improve the error-correcting performance.A multi-core layered decoder architecture that supports up to 100 Gbit/s throughput is designed based on the special protograph structure.
基金Project supported by the National Key Basic Research Program of China(Grant Nos.2015CB921000,2016YFA0300301,2017YFA0303003,and 2017YFA0302902)the National Natural Science Foundation of China(Grant Nos.11674374,11804378,and 11574372)+3 种基金the Beijing Municipal Science and Technology Project(Grant No.Z161100002116011)the Key Research Program of Frontier Sciences,Chinese Academy of Sciences(Grant Nos.QYZDB-SSW-SLH008 and QYZDY-SSW-SLH001)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB07020100)the Opening Project of Wuhan National High Magnetic Field Center(Grant No.PHMFF2015008)
文摘As an essential component of the Materials Genome Initiative aiming to shorten the period of materials research and development, combinatorial synthesis and rapid characterization technologies have been playing a more and more important role in exploring new materials and comprehensively understanding materials properties. In this review, we discuss the advantages of high-throughput experimental techniques in researches on superconductors. The evolution of combinatorial thin-film technology and several high-speed screening devices are briefly introduced. We emphasize the necessity to develop new high-throughput research modes such as a combination of high-throughput techniques and conventional methods.
基金Selected from Proceedings of the 7th International Conference on Frontiers of Design and Manufacturing (ICFDM’2006).
文摘The experiments of high throughput drilling of Ti-6Al-4V at 183 m/min cutting speed and 156 mm^3/s material removal rate using a 4 mm diameter WC-Co spiral point drill are conducted. At this material removal rate, it took only 0.57 s to drill a hole in a 6.35 mm thick Ti plate. Supplying the cutting fluid via through-the-drill holes and the balance of cutting speed and feed have proven to be critical for drill life. An inverse heat transfer model is developed to predict the heat flux and the drill temperature distribution in drilling. A three-dimensional finite element modeling of drilling is con-ducted to predict the thrust force and torque. Experimental result demonstrates that, using proper machining process parameters, tool geometry, and fine-grained WC-Co tool material, the high throughput machining of Ti alloy is technically feasible.
文摘RNA sequencing is the use of hight hroughput next generation sequencing technology to survey, characterize, and quantify the transcriptome of a genome. RNA sequencing has been used to analyze the pathogenesis of several malignancies such melanoma, lung cancer, and colorectal cancer. RNA sequencing can identify differential expression of genes(DEG's), mutated genes, fusion genes, and gene isoforms in disease states. RNA sequencing has been used in the investigation of several colorectal diseases such as colorectal cancer, inflammatory bowel disease(ulcerative colitis and Crohn's disease), and irritable bowel syndrome.
文摘This paper presents a performance study of the distributed coordination function (DCF) of 802.11 networks considering erroneous channel and capture effects under non-saturated traffic conditions employing a basic access method.The aggregate throughput of a practical wireless local area network (WLAN) strongly depends on the channel conditions.In a real radio environment,the received signal power at the access point from a station is subjected to deterministic path loss,shadowing,and fast multipath fading.The binary exponential backoff (BEB) mechanism of IEEE 802.11 DCF severely suffers from more channel idle time under high bit error rate (BER).To alleviate the low performance of IEEE 802.11 DCF,a new mechanism is introduced,which greatly outperforms the existing methods under a high BER.A multidimensional Markov chain model is used to characterize the behavior of DCF in order to account both non-ideal channel conditions and capture effects.
基金funding from National Natural Science Foundation of China (Grant No. 31601745)Natural Science Foundation of Jiangsu Province (Grant No. BK20160579)Aarhus University Research Foundation (Grant No. 30379)
文摘Our aim is to study the roles of a new emerging group of non-coding RNAs, circRNAs, in tomato(Solanum lycopersicum L.) plants grown at the combination of drought and heat, two of the most usual stress conditions known to frequently happen in field. Tomato seedlings from cultivar‘Jinling Meiyu’ were treated without stresses(control), at water shortage, high temperature and subjected the multiple stresses. In total, 467 circRNAs were identified with 87.82% from exon using high throughput sequencing technology. Among the circRNAs, 70 were from chr1 with the range from 23 to 49 from the other chromosomes. In detail, 156 circRNAs were shared in the four libraries, while 21, 17 and 36 circRNAs were only shown in drought, heat and multiple stresses libraries, respectively. Through a differential expression analysis, four, seven and nine circRNAs were differentially regulated in tomato at drought, heat and multiple stresses as compared with control. These circRNAs played roles on photosynthesis, starch and sucrose metabolism, RNA transport, RNA degradation, spliceosome, ribosome, etc. Our study underlined the potential role of circRNAs involved in the abiotic stress response in tomato, which might pave the way for studying biological roles of circRNAs responding to multiple stresses in plants.
基金Supported by Science and Technology Project from the Beijing Municipal Administration Center of Parks(ZX2018016).
文摘In order to reveal the influence of different plant configurations on the microbial community structure and diversity in rhizosphere soil of Cotinus coggygria in Fragrant Hills park,the ITS+5.8S rDNA gene and 16S rDNA gene V3-V4 region sequencing analysis for fungi and bacteria,respectively,were conducted by high throughput sequencing(Illumina MiSeq).The results showed that the fungal diversity in the rhizosphere soil samples of C.coggygria in Fragrant Hills park in 2018 was significantly higher than that in 2016,and it was higher in the rhizosphere soil of healthy C.coggygria in Xunlupo than that in diseased ones in 2018.Verticillium dahliae,which is the causal agent of C.coggygria wilt,was detected in five soil samples.In 2018,the bacterial diversity in the rhizosphere soil of diseased C.coggygria in Xunlupo was the lowest,while it was the highest in the rhizosphere soil of healthy C.coggygria under Platycladus orientalis in Langfengting.
基金the International Cooperation Project of the Ministry of Sciences and Technology of China(2014DFA32860)the National Natural Science Foundation of China(31402104)for their financial support
文摘This study was conducted to investigate the phylogenetic diversity of archaea in the rumen of adult and elderly yaks. Six domesticated female yaks, 3 adult yaks ((5.3±0.6) years old), and 3 elderly yaks ((10.7±0.6) years old), were used for the rumen contents collection. Illumina MiSeq high-throughput sequencing technology was applied to examine the archaeal composition of rumen contents. A total of 92 901 high-quality archaeal sequences were analyzed, and these were assigned to 2 033 operational taxonomic units (OTUs). Among these, 974 OTUs were unique to adult yaks while 846 OTUs were unique to elderly yaks; 213 OTUs were shared by both groups. At the phylum level, more than 99% of the obtained OTUs belonged to the Euryarchaeota phylum. At the genus level, the archaea could be divided into 7 archaeal genera. The 7 genera (i.e., Methanobrevibacter, Methanobacterium, Methanosphaera, Thermogymnomonas, Methanomicrobiu, Meth- animicrococcus and the unclassified genus) were shared by all yaks, and their total abundance accounted for 99% of the rumen archaea. The most abundant archaea in elderly and adult yaks were Methanobrevibacterand Thermogymnomonas, respectively. The abundance of Methanobacteria (class), Methanobacteriales (order), Methanobacteriaceae (family), and Methanobrevibacter (genus) in elderly yaks was significantly higher than in adult yaks. In contrast, the abundance of Ther-mogymnomonas in elderly yaks was 34% lower than in adult yaks, though the difference was not statistically significant. The difference in abundance of other archaea was not significant between the two groups. These results suggested that the structure of archaea in the rumen of yaks changed with age. This is the first study to compare the phytogenetic differences of rumen archaeal structure and composition using the yak model.
基金supported by the National Key Technology R&D Program of China(2015BAD03B02–2)Beijing Natural Science Foundation(6174047)+1 种基金earmarked fund for Modern Agro-industry Technology Research System(CARS-35)Agricultural Science and Technology Innovation Program(ASTIP-IAS02)
文摘Background: RNA editing is a co/posttranscriptional modification mechanism that increases the diversity of transcripts, with potential functional consequences. The advent of next-generation sequencing technologies has enabled the identification of RNA edits at unprecedented throughput and resolution. However, our knowledge of RNA editing in swine is still limited.Results: Here, we utilized RES-Scanner to identify RNA editing sites in the brain, subcutaneous fat, heart, liver,muscle, lung and ovary in three 180-day-old Large White gilts based on matched strand-specific RNA sequencing and whole-genome resequencing datasets. In total, we identified 74863 editing sites, and 92.1% of these sites caused adenosine-to-guanosine(A-to-G) conversion. Most A-to-G sites were located in noncoding regions and generally had low editing levels. In total, 151 A-to-G sites were detected in coding regions(CDS), including 94 sites that could lead to nonsynonymous amino acid changes. We provide further evidence supporting a previous observation that pig transcriptomes are highly editable at PRE-1 elements. The number of A-to-G editing sites ranged from 4155(muscle) to 25001(brain) across the seven tissues. The expression levels of the ADAR enzymes could explain some but not all of this variation across tissues. The functional analysis of the genes with tissuespecific editing sites in each tissue revealed that RNA editing might play important roles in tissue function.Specifically, more pathways showed significant enrichment in the fat and liver than in other tissues, while no pathway was enriched in the muscle.Conclusions: This study identified a total of 74863 nonredundant RNA editing sites in seven tissues and revealed the potential importance of RNA editing in tissue function. Our findings largely extend the porcine editome and enhance our understanding of RNA editing in swine.
基金supported by the National Natural Science Foundation of China(31972576)the Beijing Natural Science Foundation(6202004)+2 种基金the Special Program on Science and Technology Innovation Capacity Building of BAAFS(KJCX20180414 and KJCX201914)the USDA National Institute of Food and Agriculture(2018-68003-27462 and 2018-33610-28252)the Oklahoma Center for the Advancement of Science and Technology(AR19-27)。
文摘Background:In-feed antibiotics are being phased out in livestock production worldwide.Alternatives to antibiotics are urgently needed to maintain animal health and production performance.Host defense peptides(HDPs)are known for their broad-spectrum antimicrobial and immunomodulatory capabilities.Enhancing the synthesis of endogenous HDPs represents a promising antibiotic alternative strategy to disease control and prevention.Methods:To identify natural products with an ability to stimulate the synthesis of endogenous HDPs,we performed a high-throughput screening of 1261 natural products using a newly-established stable luciferase reporter cell line known as IPEC-J2/pBD3-luc.The ability of the hit compounds to induce HDP genes in porcine IPEC-J2 intestinal epithelial cells,3D4/31 macrophages,and jejunal explants were verified using RT-qPCR.Augmentation of the antibacterial activity of porcine 3D4/31 macrophages against a Gram-negative bacterium(enterotoxigenic E.coli)and a Gram-positive bacterium(Staphylococcus aureus)were further confirmed with four selected HDP-inducing compounds.Results:A total of 48 natural products with a minimum Z-score of 2.0 were identified after high-throughput screening,with 21 compounds giving at least 2-fold increase in luciferase activity in a follow-up dose-response experiment.Xanthohumol and deoxyshikonin were further found to be the most potent in inducing pBD3 mRNA expression,showing a minimum 10-fold increase in IPEC-J2,3D4/31 cells,and jejunal explants.Other compounds such as isorhapontigenin and calycosin also enhanced pBD3 mRNA expression by at least 10-fold in both IPEC-J2 cells and jejunal explants,but not 3D4/31 cells.In addition to pBD3,other porcine HDP genes such as pBD2,PG1-5,and pEP2C were induced to different magnitudes by xanthohumol,deoxyshikonin,isorhapontigenin,and calycosin,although clear gene-and cell type-specific patterns of regulation were observed.Desirably,these four compounds had a minimum effect on the expression of several representative inflammatory cytokine genes.Furthermore,when used at HDP-inducing concentrations,these compounds showed no obvious direct antibacterial activity,but significantly augmented the antibacterial activity of 3D4/31 macrophages(P<0.05)against both Gram-negative and Gram-positive bacteria.Conclusions:Our results indicate that these newly-identified natural HDP-inducing compounds have the potential to be developed as novel alternatives to antibiotics for prophylactic and therapeutic treatment of infectious diseases in livestock production.
基金Supported by the National Natural Science Foundation of China(Nos.41576123,41706129)the Guangdong Natural Science Foundation(Nos.2015A030313326,2016A030312004)+2 种基金the International Science and Technology Cooperation Project(No.GASI-IPOVI-04)the Project of Enhancing School with Innovation of Guangdong Ocean University(No.GDOU2016050243)the Program for Scientific Research Start-Up Funds of Guangdong Ocean University(No.E15030)
文摘Huguangyan Maar Lake is a typical maar lake in the southeast of China. It is well preserved and not disturbed by anthropogenic activities. In this study, microbial community structures in sediment and water samples from Huguangyan Maar Lake were investigated using a high-throughput sequencing method. We found significant differences between the microbial community compositions of the water and the sediment. The sediment samples contained more diverse Bacteria and Archaea than did the water samples. Actinobacteria, Betaproteobacteria, Cyanobacteria, and Deltaproteobacteria predominated in the water samples while Deltaproteobacteria, Anaerolineae, Nitrospira, and Dehalococcoidia were the major bacterial groups in the sediment. As for Archaea, Woesearchaeota (DHVEG-6), unclassified Archaea, and Deep Sea Euryarchaeotic Group were detected at higher abundances in the water, whereas the Miscellaneous Crenarchaeotic Group, Thermoplasmata, and Methanomicrobia were significantly more abundant in the sediment. Interactions between Bacteria and Archaea were common in both the water column and the sediment. The concentrations of major nutrients (NO^3-, PO4^3-, SiO3^2- and NH4^+) shaped the microbial population structures in the water. At the higher phylogenetic levels including phylum and class, many of the dominant groups were those that were also abundant in other lakes;however, novel microbial populations (unclassified) were often seen at the lower phylogenetic levels. Our study lays a foundation for examining microbial biogeochemical cycling in sequestered lakes or reservoirs.
基金Supported by the National Natural Science Foundations of China(3127218631301791)
文摘Gene sequencing is a great way to interpret life, and high-throughput sequencing technology is a revolutionary technological innovation in gene sequencing researches. This technology is characterized by low cost and high-throughput data. Currently, high-throughput sequencing technology has been widely applied in multi-level researches on genomics, transcriptomics and epigenomics. And it has fundamentally changed the way we approach problems in basic and translational researches and created many new possibilities. This paper presented a general description of high-throughput sequencing technology and a comprehensive review of its application with plain, concisely and precisely. In order to help researchers finish their work faster and better, promote science amateurs and understand it easier and better.
基金supported by The Project funded by China Postdoctoral Science Foundation(Grant No.2018T110505,2017M611828)The Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions。
文摘The exponentially increasing number of heterogeneous Internet of Things(Io T)devices(e.g.,Wi Fi and Zig Bee)crowed in the same ISM band(2.4 G)and recent advances in CrossTechnology Communications(CTC)motivate us to explore more efficient data collection and maximize network throughput.CTC enables Wi Fi and Zig Bee devices to communicate directly without any hardware changes or gateway equipment,which sheds light on a more efficient data collection design.In this work,we propose a distributed algorithm,named Max Bee,to compute the maximum network throughput,which is formulated as a linear programming problem.Considering that the problem turns out to be non-convex and hard to solve exactly,we propose a distributed algorithm to solve nonlinear programming by using the dual decomposition method and gradient/subgradient algorithms.Through extensive simulations on different sets of deployed Zig Bee and Wi Fi devices,we observe that the proposed algorithm significantly increases the network throughput based on CTC for Sensor Networks.
基金This work was partially supported by the National Natural Science Foundation of China(No.61771410,No.61876089)by the Postgraduate Innovation Fund Project by Southwest University of Science and Technology(No.19ycx0106)+2 种基金by the Artificial Intelligence Key Laboratory of Sichuan Province(No.2017RYY05,No.2018RYJ03)by the Zigong City Key Science and Technology Plan Project(2019YYJC16)by and by the Horizontal Project(No.HX2017134,No.HX2018264,Nos.E10203788,HX2019250).
文摘This paper investigates the power control and routing problem in the communication process of an energy harvesting(EH)multi-hop cognitive radio network(CRN).The secondary user(SU)nodes(i.e.,source node and relay nodes)harvest energy from the environment and use the energy exclusively for transmitting data.The SU nodes(i.e.,relay nodes)on the path,store and forward the received data to the destination node.We consider a real world scenario where the EH-SU node has only local causal knowledge,i.e.,at any time,each EH-SU node only has knowledge of its own EH process,channel state and currently received data.In order to study the power and routing issues,an optimization problem that maximizes path throughput considering quality of service(QoS)and available energy constraints is proposed.To solve this optimization problem,we propose a hybrid game theory routing and power control algorithm(HGRPC).The EH-SU nodes on the same path cooperate with each other,but EH-SU nodes on the different paths compete with each other.By selecting the best next hop node,we find the best strategy that can maximize throughput.In addition,we have established four steps to achieve routing,i.e.,route discovery,route selection,route reply,and route maintenance.Compared with the direct transmission,HGRPC has advantages in longer distances and higher hop counts.The algorithm generates more energy,reduces energy consumption and increases predictable residual energy.In particular,the time complexity of HGRPC is analyzed and its convergence is proved.In simulation experiments,the performance(i.e.,throughput and bit error rate(BER))of HGRPC is evaluated.Finally,experimental results show that HGRPC has higher throughput,longer network life,less latency,and lower energy consumption.