目的探讨TLR-2(Toll like receptor-2)信号途径对新生儿免疫系统在淋巴细胞增殖和调节性T细胞(Treg)扩增方面的影响及其与成人免疫系统的差别。方法应用TLR-2的配体-肽聚糖(PPG)和有丝分裂原-植物凝血素(PHA)刺激胎儿脐带血单核淋巴细胞...目的探讨TLR-2(Toll like receptor-2)信号途径对新生儿免疫系统在淋巴细胞增殖和调节性T细胞(Treg)扩增方面的影响及其与成人免疫系统的差别。方法应用TLR-2的配体-肽聚糖(PPG)和有丝分裂原-植物凝血素(PHA)刺激胎儿脐带血单核淋巴细胞(CBMC)和成人外周血单核淋巴细胞(PBMC),然后采用3H胸腺嘧啶掺入法测淋巴细胞增殖情况及流式细胞技术检测活化T细胞,Treg细胞扩增情况。结果PPG和PHA刺激后,CBMC淋巴细胞总体明显增殖(P<0.05),但PPG的刺激不能使活化T细胞和Treg细胞比例增加(P>0.05)。PPG和PHA刺激后,PBMC淋巴细胞总体明显增殖(P<0.05),且活化T细胞和Treg细胞比例均增加(P<0.05)。此外,PPG诱导的CBMC淋巴细胞增殖强度弱于PBMC(P<0.05)。结论TLR-2信号途径可以诱导新生儿淋巴细胞增殖但活化T细胞和Treg细胞比例不增加,与成人免疫系统的TLR-2信号途径存在差别。展开更多
目的研究脊柱结核患者Toll样受体-2(Toll like receptor 2,TLR-2)表达与血清中炎性因子及应激因子的相关性。方法收集2015年8月至2018年10月住院手术治疗的椎间盘突出症患者40例作为对照组,脊柱结核患者40例作为观察组。入院后采集两组...目的研究脊柱结核患者Toll样受体-2(Toll like receptor 2,TLR-2)表达与血清中炎性因子及应激因子的相关性。方法收集2015年8月至2018年10月住院手术治疗的椎间盘突出症患者40例作为对照组,脊柱结核患者40例作为观察组。入院后采集两组患者静脉血,采用免疫组化检测TLR-2表达,采用Western blot检测TLR-2蛋白表达,采用Elisa法检测血清白细胞介素1β(Interleukin,IL-1β)、白细胞介素6(Interleukin,IL-6)和C反应蛋白(C reactive protein,CRP)含量,采用Person检验分析TLR-2与IL-1β、IL-6和CRP相关性。结果观察组TLR-2表达量(0.81±0.06)显著高于对照组TLR-2表达量(0.43±0.05)(t=13.447,P<0.05);观察组TLR-2蛋白相对表达量(0.63±0.05)显著高于对照组TLR-2蛋白相对表达量(0.37±0.07)(t=46.848,P<0.05);观察组血清IL-1β(77.23±11.22)pg/ml、IL-6(61.39±13.73)pg/ml和CRP(49.29±8.98)pg/ml显著高于对照组IL-1β(48.33±12.32)pg/ml、IL-6(32.63±9.32)pg/ml和CRP(29.89±14.37)pg/ml(P<0.05);TLR-2蛋白与血清IL-1β(r=0.762,P<0.05)、IL-6(r=0.689,P<0.05)和CRP(r=0.721,P<0.05)含量之间呈正相关。结论脊柱结核血清中IL-1β、IL-6和CRP可被认为与TLR-2有密切关系,有效抑制TLR-2可有效降低脊髓结核患者的炎症水平。展开更多
[Objectives]This study aimed to explore the protective effect of Oxalis coriniculata L.on rats with acute liver injury induced by carbon tetrachloride(CCl4)and related mechanism by regulating oxidative stress and the ...[Objectives]This study aimed to explore the protective effect of Oxalis coriniculata L.on rats with acute liver injury induced by carbon tetrachloride(CCl4)and related mechanism by regulating oxidative stress and the TLR-2 TLR-2/NF-κB signaling pathway.[Methods]A total of 48 female rats were randomly and evenly divided into normal group,model group,silymarin group(0.12 g/kg),and high(16 g/kg),middle(8 g/kg)and low-dose(4 g/kg)O.coriniculata L.groups.The rats in the groups were intragastrically administered with 5 mL/kg of corresponding drugs(equal-volume distilled water for normal group and control group),respectively.The administration was conducted twice a day,for 10 consecutive days.After 2 h of the last administration,the rats in all the groups except the normal group were intraperitoneally injected with 12%carbon tetrachloride(CCl4)olive oil solution(5 mL/kg),respectively to establish liver injury rat models.After 16 h,the eyeball blood of the rats was collected,and their liver tissues were collected for preparation of HE sections.The biochemical indicators detected included aspartate aminotransferase(AST),alanine aminotransferase(ALT),total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA)content in the serum.The contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in the serum were detected by ELISA.The expression of Toll-like receptor-2(TLR-2)and nuclear factor-κB(NF-κB)in liver tissue was detected using Western blotting.The pathological changes of liver were observed under light microscope.[Results]Compared with the normal group,the ALT,AST activity and MDA,IL-1β,IL-6,TNF-αlevels in rat serum significantly increased(P<0.01),the GSH-Px,T-SOD activity in rat serum significantly decreased(P<0.01),and the expression of TLR-2 and NF-κB in liver tissue was up-regulated(P<0.01)in the model group.Compared with the model group,the ALT,AST activity and MDA,IL-1β,IL-6 and TNF-αlevels in rat serum reduced(P<0.05,P<0.01),the GSH-Px and T-SOD activity in rat serum increased(P<0.05,P<0.01),and the expression of TLR-2 and NF-κB in liver tissue was down-regulated(P<0.05,P<0.01)in the O.coriniculata L.administration groups.Pathological sections show that O.coriniculata L.had an improving effect on rats with acute liver injury induced by CCl4.[Conclusions]O.coriniculata L.has a good protective effect on rats with acute liver injury induced by CCl4.Its mechanism may be related to inhibition of oxidative stress,inhibition of inflammatory response and regulation of the TLR-2/NF-κB signaling pathway.展开更多
目的探讨Toll样受体-2(TLR-2)在人牙髓组织中的分布与表达。方法对10颗健康牙髓和10颗炎症牙髓分别进行免疫组化和逆转录实时荧光聚合酶链反应(PCR)检测,研究TLR-2的分布与表达。结果 TLR-2在健康人牙髓组织中表达呈阴性,在炎症牙髓组...目的探讨Toll样受体-2(TLR-2)在人牙髓组织中的分布与表达。方法对10颗健康牙髓和10颗炎症牙髓分别进行免疫组化和逆转录实时荧光聚合酶链反应(PCR)检测,研究TLR-2的分布与表达。结果 TLR-2在健康人牙髓组织中表达呈阴性,在炎症牙髓组织中表达呈阳性。炎症牙髓组织中TLR-2阳性表达率显著高于健康牙髓组,差异有统计学意义(P<0.01)。2组牙髓组织标本总R N A R T-PCR扩增产物电泳显示,炎症牙髓标本均在β-actin条带之上368bp位置出现条带,而健康牙髓标本未见对应条带。结论 TLR-2在炎症牙髓组织中存在弱阳性表达,提示TLR-2可能参与了人牙髓组织的炎症和免疫反应。展开更多
文摘目的探讨TLR-2(Toll like receptor-2)信号途径对新生儿免疫系统在淋巴细胞增殖和调节性T细胞(Treg)扩增方面的影响及其与成人免疫系统的差别。方法应用TLR-2的配体-肽聚糖(PPG)和有丝分裂原-植物凝血素(PHA)刺激胎儿脐带血单核淋巴细胞(CBMC)和成人外周血单核淋巴细胞(PBMC),然后采用3H胸腺嘧啶掺入法测淋巴细胞增殖情况及流式细胞技术检测活化T细胞,Treg细胞扩增情况。结果PPG和PHA刺激后,CBMC淋巴细胞总体明显增殖(P<0.05),但PPG的刺激不能使活化T细胞和Treg细胞比例增加(P>0.05)。PPG和PHA刺激后,PBMC淋巴细胞总体明显增殖(P<0.05),且活化T细胞和Treg细胞比例均增加(P<0.05)。此外,PPG诱导的CBMC淋巴细胞增殖强度弱于PBMC(P<0.05)。结论TLR-2信号途径可以诱导新生儿淋巴细胞增殖但活化T细胞和Treg细胞比例不增加,与成人免疫系统的TLR-2信号途径存在差别。
基金Supported by Bagui Scholar Program of Guangxi(Gui Cai Jiao Han[2017]No.143).
文摘[Objectives]This study aimed to explore the protective effect of Oxalis coriniculata L.on rats with acute liver injury induced by carbon tetrachloride(CCl4)and related mechanism by regulating oxidative stress and the TLR-2 TLR-2/NF-κB signaling pathway.[Methods]A total of 48 female rats were randomly and evenly divided into normal group,model group,silymarin group(0.12 g/kg),and high(16 g/kg),middle(8 g/kg)and low-dose(4 g/kg)O.coriniculata L.groups.The rats in the groups were intragastrically administered with 5 mL/kg of corresponding drugs(equal-volume distilled water for normal group and control group),respectively.The administration was conducted twice a day,for 10 consecutive days.After 2 h of the last administration,the rats in all the groups except the normal group were intraperitoneally injected with 12%carbon tetrachloride(CCl4)olive oil solution(5 mL/kg),respectively to establish liver injury rat models.After 16 h,the eyeball blood of the rats was collected,and their liver tissues were collected for preparation of HE sections.The biochemical indicators detected included aspartate aminotransferase(AST),alanine aminotransferase(ALT),total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)activity and malondialdehyde(MDA)content in the serum.The contents of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in the serum were detected by ELISA.The expression of Toll-like receptor-2(TLR-2)and nuclear factor-κB(NF-κB)in liver tissue was detected using Western blotting.The pathological changes of liver were observed under light microscope.[Results]Compared with the normal group,the ALT,AST activity and MDA,IL-1β,IL-6,TNF-αlevels in rat serum significantly increased(P<0.01),the GSH-Px,T-SOD activity in rat serum significantly decreased(P<0.01),and the expression of TLR-2 and NF-κB in liver tissue was up-regulated(P<0.01)in the model group.Compared with the model group,the ALT,AST activity and MDA,IL-1β,IL-6 and TNF-αlevels in rat serum reduced(P<0.05,P<0.01),the GSH-Px and T-SOD activity in rat serum increased(P<0.05,P<0.01),and the expression of TLR-2 and NF-κB in liver tissue was down-regulated(P<0.05,P<0.01)in the O.coriniculata L.administration groups.Pathological sections show that O.coriniculata L.had an improving effect on rats with acute liver injury induced by CCl4.[Conclusions]O.coriniculata L.has a good protective effect on rats with acute liver injury induced by CCl4.Its mechanism may be related to inhibition of oxidative stress,inhibition of inflammatory response and regulation of the TLR-2/NF-κB signaling pathway.
文摘目的探讨Toll样受体-2(TLR-2)在人牙髓组织中的分布与表达。方法对10颗健康牙髓和10颗炎症牙髓分别进行免疫组化和逆转录实时荧光聚合酶链反应(PCR)检测,研究TLR-2的分布与表达。结果 TLR-2在健康人牙髓组织中表达呈阴性,在炎症牙髓组织中表达呈阳性。炎症牙髓组织中TLR-2阳性表达率显著高于健康牙髓组,差异有统计学意义(P<0.01)。2组牙髓组织标本总R N A R T-PCR扩增产物电泳显示,炎症牙髓标本均在β-actin条带之上368bp位置出现条带,而健康牙髓标本未见对应条带。结论 TLR-2在炎症牙髓组织中存在弱阳性表达,提示TLR-2可能参与了人牙髓组织的炎症和免疫反应。
