期刊文献+
共找到4篇文章
< 1 >
每页显示 20 50 100
Expression of TN4 gene and its role in human hepatocarcinog enesis from Qidong, a liver cancer risk area 被引量:2
1
作者 陆东东 张锡然 曹祥荣 《Chinese Medical Journal》 SCIE CAS CSCD 2004年第3期440-444,共5页
Background We investigated the expression and role of TN4 in the oncogenesis of human hepatocellular carcinoma (HCC) from Qidong which is a HCC risk area. Methods The expression of TN4 in HCC was observed using immuno... Background We investigated the expression and role of TN4 in the oncogenesis of human hepatocellular carcinoma (HCC) from Qidong which is a HCC risk area. Methods The expression of TN4 in HCC was observed using immunohistochemical staining (IHC). TN4 levels were manipulated in human liver cancer cell SMMC7721, using pcDNA3.1 eukaryotic expression constructs designed to express the complete TN4 cDNA. The biological changes of the cells were observed before and after transfection of TN4 and the change of gene expression was analysed by atlas cDNA expression array. Results Among 100 pairs of samples of HCC, TN4 down-regulation expression and up-regulation expression positive rate were 81% (81/100), 19% (19/100), respectively (P<0.01). TN4 protein was mainly localized in cytoplasm and membrane. The positive rate of TN4 were 10% (3/30), 100% (70/70) in lymph node metastasis and no lymph node metastasis, respectively ( P <0.01). The growth rates of the derivative SMMC7721-TN4 cell lines were decreased in comparasion with that of normal SMMC7721 cells and pcDNA-SMMC7721. Some gene expression was changed before and after transfection of TN4. At 30 days of post-implantation of SMMC7721-TN4, SMMC7721-pcDNA3, SMMC7721 group produced tumors of (301.9 +/- 143.4) mm(3), (2418.7 +/- 362.8) mm(3), (2317.4 +/- 587.8) mm(3), respectively, ( P <0.01). Tumor inhibiting rate was 82.4% in TN4 transfection group. Sections of tumors were observed for their degree of tissue necrosis and there was higher degree of necrosis in tumors of the TN4-SMMC7721 cell group than those of the SMMC7721, SMMC7721-pcDNA groups. Conclusions TN4 may play an important role in the oncogenesis of human HCC, especially in Qidong, the HCC risk area and TN4 could be a candidate tumor suppressor gene for HCC. 展开更多
关键词 tn4 gene liver neoplasms PATHOLOGY TRANSFECTION SMMC7721 cell
原文传递
TNS4和Stat3蛋白表达与大肠癌侵袭转移的相关性研究 被引量:1
2
作者 杨朝晖 曹学全 +3 位作者 卢洪胜 孙刚 章辉 王四玲 《浙江医学》 CAS 2013年第22期1964-1966,1971,共4页
目的探讨张力蛋白4(TNS4)和信号转导和转录活化因子3(Stat3)在大肠癌中的表达情况及其临床意义。方法采用免疫组织化学En Visiob法检测90例大肠癌患者的癌组织和其中20例患者的癌旁组织中TNS4和Stat3的表达情况。结果TNS4和Stat3在大肠... 目的探讨张力蛋白4(TNS4)和信号转导和转录活化因子3(Stat3)在大肠癌中的表达情况及其临床意义。方法采用免疫组织化学En Visiob法检测90例大肠癌患者的癌组织和其中20例患者的癌旁组织中TNS4和Stat3的表达情况。结果TNS4和Stat3在大肠癌组织中阳性表达率均显著高于癌旁组织(均P<0.05)。TNS4和Stat3的阳性率在不同肿瘤浸润深度、有无淋巴结转移和不同临床分期的大肠癌患者之间均具有统计学差异(均P<0.05),而在不同年龄、性别、肿瘤大小及分化程度的患者之间无统计学差异(均P>0.05)。在大肠癌组织中两者表达呈正相关(r=-0.476,P<0.05)。结论 TNS4和Stat3的表达异常可能共同参与了大肠癌的发生、发展、侵袭和转移;联合检测以上两种蛋白的表达对于评价大肠癌的恶性程度、判断其转移潜能和预后具有一定的参考价值。 展开更多
关键词 TNS4 信号转导和转录活化因子3 大肠癌
下载PDF
TNS4在肺腺癌中的表达及预后分析
3
作者 张鑫 吴静 +4 位作者 刘亚锋 邢应如 周家伟 谢军 胡东 《安徽理工大学学报(自然科学版)》 CAS 2022年第2期102-108,共7页
目的探讨TNS4基因在肺腺癌中的诊断及预后价值,联合临床信息为肺腺癌患者构建预测模型。方法分析TNS4基因在肺腺癌组织和癌旁组织的表达情况,绘制ROC曲线探讨其诊断价值。运用单-多因素Cox分析探讨其预后价值,联合临床特点构建患者预后... 目的探讨TNS4基因在肺腺癌中的诊断及预后价值,联合临床信息为肺腺癌患者构建预测模型。方法分析TNS4基因在肺腺癌组织和癌旁组织的表达情况,绘制ROC曲线探讨其诊断价值。运用单-多因素Cox分析探讨其预后价值,联合临床特点构建患者预后临床预测模型。结果肺腺癌组织TNS4基因的表达高于癌旁组织(P<0.01),单-多因素Cox分析表明TNS4基因在肺腺癌的诊断中具有高准确性(P<0.01)。此外,研究发现TNS4低表达的癌患者预后明显好(P<0.01),构建的临床预测模型能够很好的评估肺腺癌患者3a和5a的生存率。结论TNS4基因可作为肺腺癌诊断和预后的独立生物学标志物。 展开更多
关键词 肺腺癌 TNS4 TCGA 诊断和预后 列线图 临床预测模型
下载PDF
A modified simulated annealing algorithm and an excessive area model for floorplanning using fixed-outline constraints 被引量:5
4
作者 De-xuan ZOU Gai-ge WANG +1 位作者 Gai PAN Hong-wei QI 《Frontiers of Information Technology & Electronic Engineering》 SCIE EI CSCD 2016年第11期1228-1244,共17页
Outline-free floorplanning focuses on area and wirelength reductions, which are usually meaningless, since they can hardly satisfy modern design requirements. We concentrate on a more difficult and useful issue, fixed... Outline-free floorplanning focuses on area and wirelength reductions, which are usually meaningless, since they can hardly satisfy modern design requirements. We concentrate on a more difficult and useful issue, fixed-outline floorplanning. This issue imposes fixed-outline constraints on the outline-free floorplanning, making the physical design more interesting and challenging. The contributions of this paper are primarily twofold. First, a modified simulated annealing(MSA) algorithm is proposed. In the beginning of the evolutionary process, a new attenuation formula is used to decrease the temperature slowly, to enhance MSA's global searching capacity. After a period of time, the traditional attenuation formula is employed to decrease the temperature rapidly, to maintain MSA's local searching capacity. Second, an excessive area model is designed to guide MSA to find feasible solutions readily. This can save much time for refining feasible solutions. Additionally, B*-tree representation is known as a very useful method for characterizing floorplanning. Therefore, it is employed to perform a perturbing operation for MSA. Finally, six groups of benchmark instances with different dead spaces and aspect ratios—circuits n10, n30, n50, n100, n200, and n300—are chosen to demonstrate the efficiency of our proposed method on fixed-outline floorplanning. Compared to several existing methods, the proposed method is more efficient in obtaining desirable objective function values associated with the chip area, wirelength, and fixed-outline constraints. 展开更多
关键词 Fixed-outline floorplanning Modified simulated annealing algorithm Global search Excessive area model B*-tree representation
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部