Objective:To investigate the expression characteristics of TNF-αin myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopoiysaccharide(LPS).Methods: Male SPF Wistar rats were randomly di...Objective:To investigate the expression characteristics of TNF-αin myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopoiysaccharide(LPS).Methods: Male SPF Wistar rats were randomly divided into room temperature+physiological saline group (Croup C),hyperthermia+physiological saline group(Croup II),room temperature+LPS group(Croup L) and hyperthermia+LPS group(Croup HL).The rats were put in simulated climate cabin.Croup HL and Croup H were exposed in the environment at a dry bulb temperature(TDB) of(35.0±0.5)℃,while Croup L and Croup C were exposed in the environment at a TDB of(26.0±0.5)℃. The rats in Croup HL and Croup L were given tail intravenous injection of LPS 10 mg/kg,while the rats in Croup H and Croup C were given tail intravenous injection of 9 g/L NaCl 10 ml/kg. After the stress,immunohistochemical SABC staining method was used to detect the expression characteristics of TNF-αin myocardium and hepatic tissue of rats,and those rats were given routine pathological examinations.Results:The expression of TNF-αin myocardium and hepatic tissue in Croup HL was enhanced remarkably,and the tissue damages of Croup HL were severest.Conclusions:The eardiotoxieity and hepatotoxicity caused by compound stress of hyperthermia and LPS is closely related to the expression of TNF-α.展开更多
Objective Rheumatoid arthritis(RA)is an autoimmune disease involving the synovial lining of the major joints.Current therapies have noteworthy side effects.Our study involved in silico evaluation of Ehretia laevis(E.l...Objective Rheumatoid arthritis(RA)is an autoimmune disease involving the synovial lining of the major joints.Current therapies have noteworthy side effects.Our study involved in silico evaluation of Ehretia laevis(E.laevis)phytoconstituents targeting tumor necrosis factor-α(TNF-α).Methods Molecular docking studies performed to investigate the binding pattern of the plant E.laevis phytoconstituents along with the crystal structure of TNF-α(PDB ID:2 AZ5)using AutoDock Vina followed by a study of interacting amino acid residues and their influence on the inhibitory potentials of the active constituents.Further the pharmacokinetic profile and toxicity screening carried out using Swiss ADME and pk CSM.Results The docked results suggest that lupeol(-9.4 kcal/mol)andα-amyrin(-9.4 kcal/mol)has best affinity towards TNF-αcompared to standard drug thalidomide(-7.4 kcal/mol).The active chemical constituents represents better interaction with the conserved catalytic residues,leading to the inhibition/blockade of the TNF-α-associated signaling pathway in RA.Furthermore,pharmacokinetics and toxicity parameters of these phytochemicals were within acceptable limits according to ADMET studies.Conclusion The binding potential of phytoconstituents targeting TNF-αshowed promising results.Nonetheless,it encourages the traditional use of E.laevis and provides vital information on drug development and clinical treatment.展开更多
[Objectives]The effects of Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb on serum endotoxin,NO and TNF-αlevels were investigated.[Methods]Total 105 mice were randomly divided into Dachengqi...[Objectives]The effects of Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb on serum endotoxin,NO and TNF-αlevels were investigated.[Methods]Total 105 mice were randomly divided into Dachengqi decoction made from raw rhubarb groups(6,10 g/kg),Dachengqi decoction made from vinegar-processed rhubarb groups(6,10 g/kg),positive control group,blank control group and model group.After administration at a dose of 20 mL/kg,the levels of endotoxin,NO and TNF-αin serum were determined.The effects of Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb on serum endotoxin,NO and TNF-αlevels in mice were compared.[Results]Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb both increased the NO level and reduced the endotoxin and TNF-αlevels in serum of ABP mice.[Conclusions]Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb have different effects on endotoxin,NO and TNF-αcontents.These changes correspond to the effects of Dachengqi Decoctions made from raw rhubarb and vinegar-processed rhubarb.The change in the content of rhubarb anthraquinones has a certain effect on the efficacy of Dachengqi decoction.展开更多
Objectives To determine thechange of plasma leptin levels and the correlation be-tween hyperleptinaemia and insulin resistance orTNF -α in patients with congestive heart failure(CHF). Methods The levels of plasma lep...Objectives To determine thechange of plasma leptin levels and the correlation be-tween hyperleptinaemia and insulin resistance orTNF -α in patients with congestive heart failure(CHF). Methods The levels of plasma leptin, fastingplasma insulin (FINS) and TNF-α in 64 male patientswith CHF and 30 healthy male people were measuredby ELISA. Body mass index (BMI), waist/hip ratio(WHR) and insulin resistance index (Homa-IR) werecalculated respectively. Results The plasma leptinlevels in CHF group were higher compared with con-trol group (P<0.00l). There was a increased trend ofplasma leptin levels in CHF group with the increaseof NYHA class (r_s=0.884, P<0.001), according to dif-ferent etiology,there was no significant difference ofplasma leptin levels in CHF group (P>0.05); FINS,Homa-IR and TNF-α levels in CHF group were high-er than in control group (all P<0.001); in CHF groupplasma leptin levels correlated with FINS (r=0.652, P<0.001),Homa-IR (r=0.561,P<0.001) and TNF-α (r=0.629,P<0.001); in multiple stepwise regression anal-ysis BMI, FINS and TNF-α were isolated factors af-fecting plasma leptin levels in CHF group, Y=-3.466+0.456 (BMI)+ 0.162 (FINS)+4.487(TNF-α), F=38.405,P<0.001. Conclusions plasma leptin levels wereraised and correlated with disease severity in patientswith CHF; FINS and TNF-α were isolated factor af-fecting plasma leptin level in CHF patients; hyper-leptinaemia may play a role in the pathophysiology ofCHF such as catabolic process and activation of neu-rohormonal.展开更多
文摘Objective:To investigate the expression characteristics of TNF-αin myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopoiysaccharide(LPS).Methods: Male SPF Wistar rats were randomly divided into room temperature+physiological saline group (Croup C),hyperthermia+physiological saline group(Croup II),room temperature+LPS group(Croup L) and hyperthermia+LPS group(Croup HL).The rats were put in simulated climate cabin.Croup HL and Croup H were exposed in the environment at a dry bulb temperature(TDB) of(35.0±0.5)℃,while Croup L and Croup C were exposed in the environment at a TDB of(26.0±0.5)℃. The rats in Croup HL and Croup L were given tail intravenous injection of LPS 10 mg/kg,while the rats in Croup H and Croup C were given tail intravenous injection of 9 g/L NaCl 10 ml/kg. After the stress,immunohistochemical SABC staining method was used to detect the expression characteristics of TNF-αin myocardium and hepatic tissue of rats,and those rats were given routine pathological examinations.Results:The expression of TNF-αin myocardium and hepatic tissue in Croup HL was enhanced remarkably,and the tissue damages of Croup HL were severest.Conclusions:The eardiotoxieity and hepatotoxicity caused by compound stress of hyperthermia and LPS is closely related to the expression of TNF-α.
文摘Objective Rheumatoid arthritis(RA)is an autoimmune disease involving the synovial lining of the major joints.Current therapies have noteworthy side effects.Our study involved in silico evaluation of Ehretia laevis(E.laevis)phytoconstituents targeting tumor necrosis factor-α(TNF-α).Methods Molecular docking studies performed to investigate the binding pattern of the plant E.laevis phytoconstituents along with the crystal structure of TNF-α(PDB ID:2 AZ5)using AutoDock Vina followed by a study of interacting amino acid residues and their influence on the inhibitory potentials of the active constituents.Further the pharmacokinetic profile and toxicity screening carried out using Swiss ADME and pk CSM.Results The docked results suggest that lupeol(-9.4 kcal/mol)andα-amyrin(-9.4 kcal/mol)has best affinity towards TNF-αcompared to standard drug thalidomide(-7.4 kcal/mol).The active chemical constituents represents better interaction with the conserved catalytic residues,leading to the inhibition/blockade of the TNF-α-associated signaling pathway in RA.Furthermore,pharmacokinetics and toxicity parameters of these phytochemicals were within acceptable limits according to ADMET studies.Conclusion The binding potential of phytoconstituents targeting TNF-αshowed promising results.Nonetheless,it encourages the traditional use of E.laevis and provides vital information on drug development and clinical treatment.
基金National Natural Science Foundation of China(No.81360524)Youth Foundation of Guangxi University of Chinese Medicine(No.2019QN036)+1 种基金Project for Improving Basic Scientific Research Ability of Young and Middle-aged Teachers in Colleges and Universities of Guangxi in 2019(No.2019KY0341)Traditional Chinese Medicine Scientific Research Laboratory(Grade III)of National Administration of Traditional Chinese Medicine:Laboratory of Chinese(Zhuang)Medicine Chemical and Quality Analysis(Guo Zhong Yi Yao Fa 2009[21]).
文摘[Objectives]The effects of Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb on serum endotoxin,NO and TNF-αlevels were investigated.[Methods]Total 105 mice were randomly divided into Dachengqi decoction made from raw rhubarb groups(6,10 g/kg),Dachengqi decoction made from vinegar-processed rhubarb groups(6,10 g/kg),positive control group,blank control group and model group.After administration at a dose of 20 mL/kg,the levels of endotoxin,NO and TNF-αin serum were determined.The effects of Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb on serum endotoxin,NO and TNF-αlevels in mice were compared.[Results]Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb both increased the NO level and reduced the endotoxin and TNF-αlevels in serum of ABP mice.[Conclusions]Dachengqi decoctions made from raw rhubarb and vinegar-processed rhubarb have different effects on endotoxin,NO and TNF-αcontents.These changes correspond to the effects of Dachengqi Decoctions made from raw rhubarb and vinegar-processed rhubarb.The change in the content of rhubarb anthraquinones has a certain effect on the efficacy of Dachengqi decoction.
文摘Objectives To determine thechange of plasma leptin levels and the correlation be-tween hyperleptinaemia and insulin resistance orTNF -α in patients with congestive heart failure(CHF). Methods The levels of plasma leptin, fastingplasma insulin (FINS) and TNF-α in 64 male patientswith CHF and 30 healthy male people were measuredby ELISA. Body mass index (BMI), waist/hip ratio(WHR) and insulin resistance index (Homa-IR) werecalculated respectively. Results The plasma leptinlevels in CHF group were higher compared with con-trol group (P<0.00l). There was a increased trend ofplasma leptin levels in CHF group with the increaseof NYHA class (r_s=0.884, P<0.001), according to dif-ferent etiology,there was no significant difference ofplasma leptin levels in CHF group (P>0.05); FINS,Homa-IR and TNF-α levels in CHF group were high-er than in control group (all P<0.001); in CHF groupplasma leptin levels correlated with FINS (r=0.652, P<0.001),Homa-IR (r=0.561,P<0.001) and TNF-α (r=0.629,P<0.001); in multiple stepwise regression anal-ysis BMI, FINS and TNF-α were isolated factors af-fecting plasma leptin levels in CHF group, Y=-3.466+0.456 (BMI)+ 0.162 (FINS)+4.487(TNF-α), F=38.405,P<0.001. Conclusions plasma leptin levels wereraised and correlated with disease severity in patientswith CHF; FINS and TNF-α were isolated factor af-fecting plasma leptin level in CHF patients; hyper-leptinaemia may play a role in the pathophysiology ofCHF such as catabolic process and activation of neu-rohormonal.
