目的探讨Tnn基因对小鼠听觉功能的影响。方法①取2月龄、3月龄的C57BL/6野生型(wild-type,WT)小鼠各3只进行耳蜗冰冻切片及免疫荧光染色,观察Tnn基因在小鼠耳蜗的表达;②各取8只野生型WT小鼠及Tnn基因敲除小鼠(Tnn+/-小鼠)在1月龄、2月...目的探讨Tnn基因对小鼠听觉功能的影响。方法①取2月龄、3月龄的C57BL/6野生型(wild-type,WT)小鼠各3只进行耳蜗冰冻切片及免疫荧光染色,观察Tnn基因在小鼠耳蜗的表达;②各取8只野生型WT小鼠及Tnn基因敲除小鼠(Tnn+/-小鼠)在1月龄、2月龄、3月龄进行短声(click)及短纯音(tome burst,tb),听性脑干反应(ABR)测试并比较结果。结果①免疫荧光显示Tnn基因在2月龄、3月龄WT小鼠耳蜗中均有表达,且主要表达于螺旋神经节神经元,与Ⅲ型β-tubulin共定位;②Tnn+/-小鼠在3月龄时,click-ABR阈值较WT型升高小鼠(分别为41.88±9.64和23.13±2.59 dB SPL,P<0.01),tb-ABR阈值4、8、16、24、32 kHz(分别为45.00±17.73、44.38±18.60、45.63±18.60、50.00±20.70、54.38±19.90 dB SPL)较WT小鼠(分别为21.25±2.31、21.87±2.59、21.25±2.31、22.50±2.67、24.38±1.77 dB SPL)均升高(P<0.05或P<0.01)。结论Tnn基因对维持小鼠听功能有重要作用,该蛋白表达异常可使小鼠听功能受损,推测其可能影响螺旋神经节神经元的正常发育,影响神经元细胞的迁移或神经突向外生长,从而引起听觉功能的异常。展开更多
Low hip bone mineral density(BMD)is an important index for osteoporosis and is associated with hip fracture,which leads to more cases of disability and mortality than all other kinds of fractures(Kanis et al.,2007).BM...Low hip bone mineral density(BMD)is an important index for osteoporosis and is associated with hip fracture,which leads to more cases of disability and mortality than all other kinds of fractures(Kanis et al.,2007).BMD’s heritability is more than 60%(Arden et al.,1996).A number of candidate loci for BMD have been previously identified by Genome Wide Association Studies(GWAS)(Xiong et al.,2009;Karasik et al.,2010;Zhang et al.,2013).Nevertheless,many significant signals based on GWAS are展开更多
Due to the demand of data processing for polar ice radar in our laboratory, a Curvelet Thresholding Neural Network (TNN) noise reduction method is proposed, and a new threshold function with infinite-order continuous ...Due to the demand of data processing for polar ice radar in our laboratory, a Curvelet Thresholding Neural Network (TNN) noise reduction method is proposed, and a new threshold function with infinite-order continuous derivative is constructed. The method is based on TNN model. In the learning process of TNN, the gradient descent method is adopted to solve the adaptive optimal thresholds of different scales and directions in Curvelet domain, and to achieve an optimal mean square error performance. In this paper, the specific implementation steps are presented, and the superiority of this method is verified by simulation. Finally, the proposed method is used to process the ice radar data obtained during the 28th Chinese National Antarctic Research Expedition in the region of Zhongshan Station, Antarctica. Experimental results show that the proposed method can reduce the noise effectively, while preserving the edge of the ice layers.展开更多
文摘目的探讨Tnn基因对小鼠听觉功能的影响。方法①取2月龄、3月龄的C57BL/6野生型(wild-type,WT)小鼠各3只进行耳蜗冰冻切片及免疫荧光染色,观察Tnn基因在小鼠耳蜗的表达;②各取8只野生型WT小鼠及Tnn基因敲除小鼠(Tnn+/-小鼠)在1月龄、2月龄、3月龄进行短声(click)及短纯音(tome burst,tb),听性脑干反应(ABR)测试并比较结果。结果①免疫荧光显示Tnn基因在2月龄、3月龄WT小鼠耳蜗中均有表达,且主要表达于螺旋神经节神经元,与Ⅲ型β-tubulin共定位;②Tnn+/-小鼠在3月龄时,click-ABR阈值较WT型升高小鼠(分别为41.88±9.64和23.13±2.59 dB SPL,P<0.01),tb-ABR阈值4、8、16、24、32 kHz(分别为45.00±17.73、44.38±18.60、45.63±18.60、50.00±20.70、54.38±19.90 dB SPL)较WT小鼠(分别为21.25±2.31、21.87±2.59、21.25±2.31、22.50±2.67、24.38±1.77 dB SPL)均升高(P<0.05或P<0.01)。结论Tnn基因对维持小鼠听功能有重要作用,该蛋白表达异常可使小鼠听功能受损,推测其可能影响螺旋神经节神经元的正常发育,影响神经元细胞的迁移或神经突向外生长,从而引起听觉功能的异常。
基金partially supported by or benefited from grants from NIH (P50AR055081,R01AG026564,R01AR050496,R01AR059781,D43TW009107,P20GM109036,R01GM109068,R01MH104680,R01MH107354,R01AR057049,and R03TW008221)
文摘Low hip bone mineral density(BMD)is an important index for osteoporosis and is associated with hip fracture,which leads to more cases of disability and mortality than all other kinds of fractures(Kanis et al.,2007).BMD’s heritability is more than 60%(Arden et al.,1996).A number of candidate loci for BMD have been previously identified by Genome Wide Association Studies(GWAS)(Xiong et al.,2009;Karasik et al.,2010;Zhang et al.,2013).Nevertheless,many significant signals based on GWAS are
基金Supported by the National High Technology Research and Development Program of China (No. 2011AA040202)the National Natural Science Foundation of China (No. 40976114)
文摘Due to the demand of data processing for polar ice radar in our laboratory, a Curvelet Thresholding Neural Network (TNN) noise reduction method is proposed, and a new threshold function with infinite-order continuous derivative is constructed. The method is based on TNN model. In the learning process of TNN, the gradient descent method is adopted to solve the adaptive optimal thresholds of different scales and directions in Curvelet domain, and to achieve an optimal mean square error performance. In this paper, the specific implementation steps are presented, and the superiority of this method is verified by simulation. Finally, the proposed method is used to process the ice radar data obtained during the 28th Chinese National Antarctic Research Expedition in the region of Zhongshan Station, Antarctica. Experimental results show that the proposed method can reduce the noise effectively, while preserving the edge of the ice layers.
