[目的]建立基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI-TOF MS)技术检测不同禽肉动物源性成分的方法,构建不同禽肉蛋白质分子指纹图谱标准数据库,对不同...[目的]建立基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI-TOF MS)技术检测不同禽肉动物源性成分的方法,构建不同禽肉蛋白质分子指纹图谱标准数据库,对不同禽肉进行鉴别。[方法]样品用磷酸盐缓冲液提取,净化后用MALDI-TOF MS法测定。采用ChemPattern软件对不同禽肉蛋白图谱进行化学计量学分析,建立识别模型。[结果]鸡、鸭、鹅3种不同禽肉之间具有明显差异。首次建立不同禽肉蛋白质分子指纹图谱标准数据库,可一次性对每个样品中鸡、鸭、鹅等肉源性成分进行同时分析。[结论]建立的基于蛋白质组学的鸡、鸭、鹅源性成分鉴定系统具有简便、稳定、准确、重现性好的优点,适用于不同禽肉中鸡鸭鹅动物源性成分的鉴别。展开更多
Detecting feature points on the human body in video frames is a key step for tracking human movements. There have been methods developed that leverage models of human pose and classification of pixels of the body imag...Detecting feature points on the human body in video frames is a key step for tracking human movements. There have been methods developed that leverage models of human pose and classification of pixels of the body image. Yet, occlusion and robustness are still open challenges. In this paper, we present an automatic, model-free feature point detection and action tracking method using a time-of-flight camera. Our method automatically detects feature points for movement abstraction. To overcome errors caused by miss-detection and occlusion, a refinement method is devised that uses the trajectory of the feature points to correct the erroneous detections. Experiments were conducted using videos acquired with a Microsoft Kinect camera and a publicly available video set and comparisons were conducted with the state-of-the-art methods. The results demonstrated that our proposed method delivered improved and reliable performance with an average accuracy in the range of 90 %.The trajectorybased refinement also demonstrated satisfactory effectiveness that recovers the detection with a success rate of 93.7 %. Our method processed a frame in an average time of 71.1 ms.展开更多
With the expansion of herbal medicine （HM） market, the issue on how to apply up-to- date analytical tools on qualitative analysis of HMs to assure their quality, safety and efficacy has been arousing great attention...With the expansion of herbal medicine （HM） market, the issue on how to apply up-to- date analytical tools on qualitative analysis of HMs to assure their quality, safety and efficacy has been arousing great attention. Due to its inherent characteristics of accurate mass measurements and multiple stages analysis, the integrated strategy of liquid chromatography （LC） coupled with time-of-flight mass spectrometry （TOF-MS） and ion trap mass spectrometry （IT-MS） is well-suited to be performed as qualitative analysis tool in this field. The purpose of this review is to provide an overview on the potential of this integrated strategy, including the review of general features of LC-IT-MS and LC-TOF-MS, the advantages of their combination, the common procedures for structure elucidation, the potential of LC-hybrid-IT-TOF/MS and also the summary and discussion of the applications of the integrated strategy for HM qualitative analysis （2006-2011）. The advantages and future developments of LC coupled with IT and TOF-MS are highlighted.展开更多
文摘Detecting feature points on the human body in video frames is a key step for tracking human movements. There have been methods developed that leverage models of human pose and classification of pixels of the body image. Yet, occlusion and robustness are still open challenges. In this paper, we present an automatic, model-free feature point detection and action tracking method using a time-of-flight camera. Our method automatically detects feature points for movement abstraction. To overcome errors caused by miss-detection and occlusion, a refinement method is devised that uses the trajectory of the feature points to correct the erroneous detections. Experiments were conducted using videos acquired with a Microsoft Kinect camera and a publicly available video set and comparisons were conducted with the state-of-the-art methods. The results demonstrated that our proposed method delivered improved and reliable performance with an average accuracy in the range of 90 %.The trajectorybased refinement also demonstrated satisfactory effectiveness that recovers the detection with a success rate of 93.7 %. Our method processed a frame in an average time of 71.1 ms.
文摘With the expansion of herbal medicine （HM） market, the issue on how to apply up-to- date analytical tools on qualitative analysis of HMs to assure their quality, safety and efficacy has been arousing great attention. Due to its inherent characteristics of accurate mass measurements and multiple stages analysis, the integrated strategy of liquid chromatography （LC） coupled with time-of-flight mass spectrometry （TOF-MS） and ion trap mass spectrometry （IT-MS） is well-suited to be performed as qualitative analysis tool in this field. The purpose of this review is to provide an overview on the potential of this integrated strategy, including the review of general features of LC-IT-MS and LC-TOF-MS, the advantages of their combination, the common procedures for structure elucidation, the potential of LC-hybrid-IT-TOF/MS and also the summary and discussion of the applications of the integrated strategy for HM qualitative analysis （2006-2011）. The advantages and future developments of LC coupled with IT and TOF-MS are highlighted.
