Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and apoptosis of human hepatocarcinoma HepG-2 cells.Methods: Cells proliferation was assessed by MTT assay,c...Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and apoptosis of human hepatocarcinoma HepG-2 cells.Methods: Cells proliferation was assessed by MTT assay,cells morphologic was observed by the inverted microscopy,Annexin V/PI stain was used to detect the apoptosis and necrosis of the tumor cells.The expression of TOPO I mRNA and TOPO II mRNA were examined by RT-PCR.Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in doseand time-dependent ways.After Cinobufacini injection intervention,HepG-2 cells showed typical morphological changes: cells changed from polygon into round,chromatin looseness and karyolysis were observed.The percentages of apoptosis were 88.49%,76.02%,61.73% corresponding to the 48 h interference of 0.42 μg/mL,0.21 μg/mL,0.105 μg/mL Cinobufacini injection,perspectively.RT-PCR assay showed that Cinobufacini injection down-regulated TOPO I and TOPO II expression at mRNA level.Conclusion: Cinobufacini can inhibit human hepatocarcinoma HepG-2 cells growth and induce tumor cells apoptosis,the mechanism of which might partly relate to the down-regulation of TOPO I mRNA and TOPO II mRNA induced by Cinobufacini injection.展开更多
文摘Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and apoptosis of human hepatocarcinoma HepG-2 cells.Methods: Cells proliferation was assessed by MTT assay,cells morphologic was observed by the inverted microscopy,Annexin V/PI stain was used to detect the apoptosis and necrosis of the tumor cells.The expression of TOPO I mRNA and TOPO II mRNA were examined by RT-PCR.Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in doseand time-dependent ways.After Cinobufacini injection intervention,HepG-2 cells showed typical morphological changes: cells changed from polygon into round,chromatin looseness and karyolysis were observed.The percentages of apoptosis were 88.49%,76.02%,61.73% corresponding to the 48 h interference of 0.42 μg/mL,0.21 μg/mL,0.105 μg/mL Cinobufacini injection,perspectively.RT-PCR assay showed that Cinobufacini injection down-regulated TOPO I and TOPO II expression at mRNA level.Conclusion: Cinobufacini can inhibit human hepatocarcinoma HepG-2 cells growth and induce tumor cells apoptosis,the mechanism of which might partly relate to the down-regulation of TOPO I mRNA and TOPO II mRNA induced by Cinobufacini injection.
