Construction of HA-1-DC Nucleic-acid Vaccine and Induction of Specific Cytotoxic T Lymphocytes

An HA-1-DC nucleic-acid vaccine was constructed to induce anti-leukemia effect after hematopoietic stem cell transplantation (HSCT). DCs were generated from HSCT donors in vitro, and its immunologic activity was assayed by using flow cytometry and mixed lymphocytes reaction. HA-1 gene was electroporated into the cultured DCs to construct a DC nucleic-acid vaccine. After transfection for 48 h, the expression of HA-1 protein could be detected by using Western blot. The DCs were cultured with syngenic lymphocytes to induce specific cytotoxic T lymphocytes (CTLs). The cytoxicity of the CTLs was detected by LDH assay. The results showed that The DCs derived from peripheral blood monocytes (PBMCs) expressed the phenotype of DCs, and were effective in stimulating proliferation of the allogenic lymphocytes. After electroporating for 48-h, HA-1 protein was detected by using Western blot. The cytotoxity of inducing CTLs was higher than the control group. It was suggested that minor histocompatibility antigen HA-1 could be considered as a target of immunotherapy against leukemia after HSCT.

Intriguing anti-superbug Cu2O@ZrP hybrid nanosheet with enhanced antibacterial performance and weak cytotoxicity

In view of it's strong antibacterial function and minor toxicity,cuprous oxide (Cu2O) is frequently used in various broad-spectrum antibacterial reagents.Nonetheless the undesirable effects of superbugs still remain challenging.In this research,a chemical deposition approach is used to prepare a Cu2O@ZrP composite with nanosheet configuration demonstrating excellent dispersibility and antibacterial traits.From systematic analysis,it was inffered that the content of copper in the nanosheet was about 57-188 mg/g while the average thickness of the nanosheets Cu2O formed on ZrP is approximately 0.8 nm.The results of the minimal inhibitory concentration (MIC) revealed that an extremely low loading of Cu2O in Cu2O@ZrP nanosheet can lead to exceptional antibacterial activity.Examined on two various superbugs;i.e.methicillin-resistant staphylococcus aureus (MRSA) and vancomycin-resistant enterococcus (VRE),the composite nanosheet reagent performed over 99% microbial reduction.More intesetingly,the cell growth rate of the Cu2O@ZrP nanosheet was determined to be 20% lower than that of the neat Cu2O,manifesting a weaker cytotoxicity.This unique hybrid nanosheet with intriguing anti-superbug performance promises highly efficient protection for the fabrics,battledress,and medical textiles.

微量液体克隆形成法体外检测三尖杉酯碱、高三尖杉酯碱对CFU—GM及L—CFU的细胞毒作用

基于琼脂半固体培养法和极限稀释微孔池培养法,建立了一种新的体外药敏检测体系——微量液体克隆形成法。并应用该体系检测了三尖杉酯碱(H)和高三尖杉酯碱(HH)对CFU—GM及L—CFU的细胞毒作用。结果提示:24h及168h作用法时,HH对CFU—GM以及L—CFU的细胞毒作用均大于H,H、HH对L—CFO的细胞毒作用选择性均大于CFU—GM,但HH的细胞毒作用选择性不及H;H、HH的TDI(Time—schedule Depne—dence Index),IC_(90-24h)÷IC_(-90-168h)分别为68、60,说明两药物均为细胞周期时相特异性药物;并对H、HH之间可能存在的同系物间的交叉耐药性作了探讨。

Bleaching with the Mixed Adsorbents of Activated Earth and Activated Alumina to Reduce Color and Oxidation Products of Anchovy Oil

Fish oil is a rich source of polyunsaturated fatty acids,and its refinement has drawn attention for years.An appropriate adsorbent can effectively remove the pigment impurities in the fish oil.This study evaluated the impact of different absorbents on the reduction of oxidation products and color of anchovy oil during the decolorization under high vacuum.Using the single factor design,four process parameters including adsorbents type,adsorbent amount,temperature and time were tested to determine the optimum decolorization parameter.The results showed the optimum decolorization conditions were that the fish oil was treated with 8%acti-vated alumina at 80℃for 40 min.In the central group experiment,the addition amounts of mixed absorbents(activated earth and activated alumina),including the mass ratio of adsorbent in oil(5%-11%,w/w)and the mass ratio of activated earth in total absor-bent(20%-80%,w/w)were optimized to remove the oxidation products.Under the optimum condition at 10.18%of adsorbent and 70%of activated earth,the total oxidation value(TOTOX value)showed the minimum with the 44.4%of removal rate.Eight metal elements were analyzed in decolorized oil using inductively coupled plasma mass spectrometry(ICP-MS).The removal rates of Zn and Pb were 94.12%and 55.35%,respectively.The decolorization process using mixed absorbents under appropriate condition can significantly reduce the oxidation products and pigments in fish oil,which will benefit the industrial production of fish oil.

231例氨基甙类抗生素致聋患者脑干听觉诱发电位分析

目的 了解氨基甙类抗生素致聋的临床情况。方法 回顾自 1980年～ 2 0 0 1年收治的 2 31例氨基甙类抗生素致聋患者的临床资料和脑干听觉诱发电位变化。结果 6岁以下患儿 112例 (48.0 5 % ) ,～ 14岁 87例 (38.0 9% ) ,15岁以上者仅 32例 (13.86% ) ;全部患者的脑干听觉诱发电位均有不同程度的异常 ,多数小儿脑干听觉诱发电位明显异常。结论 部分聋儿有明显的残余听力 。

Immune Control of Equine Infectious Anemia Virus Infection by Cell-Mediated and Humoral Responses

Equine Infectious Anemia Virus (EIAV) is a retrovirus that establishes a persistent infection in horses and ponies. The virus is in the same lentivirus subgroup that includes human immunodeficiency virus (HIV). The similarities between these two viruses make the study of the immune response to EIAV relevant to research on HIV. We developed a mathematical model of within-host EIAV infection dynamics that contains both humoral and cell-mediated immune responses. Analysis of the model yields results on thresholds that would be necessary for a combined immune response to successfully control infection. Numerical simulations are presented to illustrate the results. These findings have the potential to lead to immunological control measures for lentiviral infection.

晶态SiO_2的细胞毒性与Ca^(2+)的关系

本文应用Fura-2荧光染料技术和测定细胞存活率的方法研见了晶态S_iO_2的细胞毒性与C_a^(2+)的关系。结果表明:在含C_a^(2+)介质中,晶态SiO_2的毒性作用,使细胞内游离C_a^(2+)浓度(〔C_a^(2+)〕_i)升高。SiO_2粉尘浓度越大,或作用时间越长,〔C_a^(2+)〕_i升高越大。在无C_a^(2+)介质中,未观察到〔C_a^(2+)〕_i的明显变化。〔C_a^(2+)〕_i的升高与细胞存活率降低是相一致的。

Effects of aqueous extracts of Aconitum carmichaeli,Rhizoma bolbostemmatis,Phytolacca acinosa,Panax notoginseng and Gekko swinhonis Gūenther on Bel-7402 cells

AIM: To investigate the anticancer activity of a chinese medical mixture, WRCP (warming and relieving Cold Phlegm), on hepatocarcinoma Bel-7402 cells. METHODS: Fingerprints of WRCP, which were composed of aqueous extracts of Aconitum carmichaeli, Rhizoma bolbostemmatis, Phytolacca acinosa, Panax notoginseng and Gekko swinhonis Gūenther, and aconitine, which could be isolated from Aconitum carmichaeli and have the potential toxicity, were identified by high pressure liquid chromatography. Bel-7402 cells were grown in the presence of WRCP, As2O3 or all-trans-retinoic acid (ATRA). Cell proliferation and viability were determined by trypan blue stain. Apoptosis and cell cycle of Bel-7402 cells were detected by flow cytometry. Morphologic and ultrastructural variations were determined under optic and electronic microscopy. The secretion of alpha-fetoprotein and albumin was detected by radioimmunoassay. RESULTS: The average quality of aconitine is 1.15 ± 0.10 μg per 7.5 g extracts. WRCP could suppress the proliferation and viability of Bel-7402 cells. The percentage of apoptosis cells and S phase cells increased on WRCP-treated cells. Treated with WRCP, Bel-7402 cells showed ultrastructural features of differentiation. The alpha-fetoprotein secretion decreased while the albumin secretion increased (P < 0.001, P < 0.001, respectively) markedly in WRCP-treated cells.CONCLUSION: WRCP can affect the proliferation, differentiation and apoptosis of Bel-7402 cells. It can arrest cells in S phase and has strong cytotoxicity to Bel-7402 cells.

2种酚类抗氧化剂对牡丹籽油氧化稳定性影响研究

通过Schaal烘箱实验,以过氧化值(PV)、茴香胺值(ρ-AnV)、全氧化值及脂肪酸组成为指标研究2种天然酚类抗氧化剂咖啡酸和阿魏酸及合成抗氧化剂TBHQ对牡丹籽油氧化稳定性影响,并建立牡丹籽油氧化动力学模型,以Rancimat法预测其货架期。研究表明:0.02%咖啡酸和0.02%阿魏酸对牡丹籽油都有一定的抗氧化效果,与不添加抗氧化剂相比货架期延长2.65、2.55倍,但较TBHQ仍有提升空间,几种抗氧化剂能力大小为TBHQ>咖啡酸>阿魏酸。咖啡酸、阿魏酸能够有效延缓牡丹籽油中α-亚麻酸等不饱和脂肪酸分解,提高牡丹籽油氧化稳定性和产品质量,能够作为高效、安全的天然抗氧化剂应用于油脂工业。

温石棉与其3种天然代用纤维矿物粉尘体外细胞毒性的对比研究

目的比较温石棉与3种天然代用矿物纤维粉尘的体外细胞毒性,评价3种粉尘安全性。方法首先比较温石棉及代用纤维的化学组分、在悬液中的性质,其次进行巨噬细胞培养,以细胞死亡率、乳酸脱氢酶及超氧化物歧化酶活力及丙二醛含量变化来观察温石棉与代用纤维的毒性。结果温石棉与纤维水镁石因含较高的OH-表现出较强的细胞毒性,纤维坡缕石含较低的OH-表现出较低的细胞毒性,斜发沸石未含有OH-而未表现出细胞毒性。结论在温石棉的3种代用纤维中,斜发沸石对肺泡巨噬细胞是安全的。