Parkinson＇s disease （PD） is the second most common neurodegenerative disorder characterized by a selective loss of nigrostriatal dopaminergic neurons. Clinical manifestations of this complex disease include resting...Parkinson＇s disease （PD） is the second most common neurodegenerative disorder characterized by a selective loss of nigrostriatal dopaminergic neurons. Clinical manifestations of this complex disease include resting tremor, bradykinesia, postural instability, gait difficulty and rigidity. Approximately 5%-10% of patients have genetic factors, yet etiology of PD remains unclear. Genetic deficits, environmental exposure, oxidative stress, mitochondrial dysfunction,展开更多
研究利用在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)对苹果种质资源的26份材料进行了SSR标记的鉴定,5对引物共获得52个等位基因,平均10.4个...研究利用在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)对苹果种质资源的26份材料进行了SSR标记的鉴定,5对引物共获得52个等位基因,平均10.4个。引物对CH04h02可区分全部的供试品种,5对引物多态性好。26份材料5个SSR位点的遗传多样性、多态性信息含量和位点杂合度的变化为0.6620～0.9149、0.6326～0.9086和0.6538～1.0000。这种方法具有经济、灵敏、高效等优点,在苹果品种鉴定中得到成功应用。展开更多
遗传多样性分析是种质资源研究的重要组成部分。由于研究群体通常都较大,简便可靠的高通量分析技术显得尤为重要。本文概述了在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repe...遗传多样性分析是种质资源研究的重要组成部分。由于研究群体通常都较大,简便可靠的高通量分析技术显得尤为重要。本文概述了在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)的发展历程、反应原理和应用,并比较了它与普通银染、琼脂糖检测以及常规荧光检测体系在SSR扩增产物检测上的优劣,讨论了该技术在植物遗传多样性研究中的应用潜力。展开更多
A self-tuning reaching law based sliding mode control(SMC)theory is proposed to stabilize the nonlinear continuous stirred tank reactor(CSTR).T-S fuzzy logic is used to build a global fuzzy state-space linear model.Co...A self-tuning reaching law based sliding mode control(SMC)theory is proposed to stabilize the nonlinear continuous stirred tank reactor(CSTR).T-S fuzzy logic is used to build a global fuzzy state-space linear model.Combing the traits of SMC and CSTR,three fuzzy rules can meet the requirements of controlled system.The self-tuning switch control law which can drive the state variables to the sliding surface as soon as possible is designed to ensure the robustness of uncertain fuzzy system.Lyapunov equation is applied to proving the stability of the sliding surface.The simulations show that the proposed approach can achieve desired performance with less chattering problem.展开更多
基金This work was supported by grants from the Major State Basic Research Development Program of China （973 Program） （No. 2006cb500700）, the National ＂863＂ High-Tech Research and Development Program of China （No. 2006AA02A408）, and National Natural Science Foundation of China （No. 30370565, 30570638 and 30770735）.
文摘Parkinson＇s disease （PD） is the second most common neurodegenerative disorder characterized by a selective loss of nigrostriatal dopaminergic neurons. Clinical manifestations of this complex disease include resting tremor, bradykinesia, postural instability, gait difficulty and rigidity. Approximately 5%-10% of patients have genetic factors, yet etiology of PD remains unclear. Genetic deficits, environmental exposure, oxidative stress, mitochondrial dysfunction,
文摘研究利用在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)对苹果种质资源的26份材料进行了SSR标记的鉴定,5对引物共获得52个等位基因,平均10.4个。引物对CH04h02可区分全部的供试品种,5对引物多态性好。26份材料5个SSR位点的遗传多样性、多态性信息含量和位点杂合度的变化为0.6620～0.9149、0.6326～0.9086和0.6538～1.0000。这种方法具有经济、灵敏、高效等优点,在苹果品种鉴定中得到成功应用。
文摘遗传多样性分析是种质资源研究的重要组成部分。由于研究群体通常都较大,简便可靠的高通量分析技术显得尤为重要。本文概述了在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)的发展历程、反应原理和应用,并比较了它与普通银染、琼脂糖检测以及常规荧光检测体系在SSR扩增产物检测上的优劣,讨论了该技术在植物遗传多样性研究中的应用潜力。
文摘A self-tuning reaching law based sliding mode control(SMC)theory is proposed to stabilize the nonlinear continuous stirred tank reactor(CSTR).T-S fuzzy logic is used to build a global fuzzy state-space linear model.Combing the traits of SMC and CSTR,three fuzzy rules can meet the requirements of controlled system.The self-tuning switch control law which can drive the state variables to the sliding surface as soon as possible is designed to ensure the robustness of uncertain fuzzy system.Lyapunov equation is applied to proving the stability of the sliding surface.The simulations show that the proposed approach can achieve desired performance with less chattering problem.
