目的探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)在大肠癌中的表达水平及其意义。方法采用SYBR Green I荧光定量RT-PCR技术对40例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA的表达进行检测,并分析其表达情况与临床病理资...目的探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)在大肠癌中的表达水平及其意义。方法采用SYBR Green I荧光定量RT-PCR技术对40例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA的表达进行检测,并分析其表达情况与临床病理资料的相关性。结果癌组织中TPEF mRNA的相对表达量明显低于正常组织(P<0.05);合并淋巴结转移的组织中表达量亦低于无淋巴结转移的组织(P<0.05)。结论TPEF基因在大肠癌组织中低表达,与大肠癌的转移相关,可能作为大肠癌恶性潜能的检测指标之一。展开更多
目的:探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)、信号转导子和转录激活子1(STAT1)在大肠癌中的表达水平及其意义。方法:采用SYBR Green I荧光定量RT-PCR技术对50例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA及STAT1 mRN...目的:探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)、信号转导子和转录激活子1(STAT1)在大肠癌中的表达水平及其意义。方法:采用SYBR Green I荧光定量RT-PCR技术对50例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA及STAT1 mRNA的表达进行检测,并分析其表达情况与临床病理资料的相关性。结果:癌组织中TPEFmRNA及STAT1 mRNA的相对表达水平明显低于相应正常组织(P<0.05);肿瘤TNM分期中T3/T4癌组织表达水平明显低于T1/T2癌组织(P<0.05);合并淋巴结转移的组织中表达水平亦低于无淋巴结转移者(P<0.05);TPEFmRNA与STAT1mRNA的表达水平呈正相关(r_(癌组织)=0.537,r_(正常组织)=0.851,P<0.001)。结论:TPEF、STAT1基因在大肠癌组织中低表达,与大肠癌的转移相关,可作为大肠癌恶性潜能的检测指标之一。展开更多
Two carbazole-based polymerization initiators possessing blue fluorescence emission have been synthesized via Wittig reaction in the solid phase at room temperature.Two-photon excited fluorescence(TPEF) spectra for th...Two carbazole-based polymerization initiators possessing blue fluorescence emission have been synthesized via Wittig reaction in the solid phase at room temperature.Two-photon excited fluorescence(TPEF) spectra for them were investigated under 800 nm fs laser pulse and two-photon absorption cross sections were determined by the Z-scan technique.Then two-photon initiating polymerization(TPIP) microfabrication experiments were successfully carried out.Three-dimensional lattice and artificial defects were gained,indicating that they were viable candidates for the two-photon polymerization initiator in practical application of microfabrication.展开更多
文摘目的探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)在大肠癌中的表达水平及其意义。方法采用SYBR Green I荧光定量RT-PCR技术对40例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA的表达进行检测,并分析其表达情况与临床病理资料的相关性。结果癌组织中TPEF mRNA的相对表达量明显低于正常组织(P<0.05);合并淋巴结转移的组织中表达量亦低于无淋巴结转移的组织(P<0.05)。结论TPEF基因在大肠癌组织中低表达,与大肠癌的转移相关,可能作为大肠癌恶性潜能的检测指标之一。
文摘目的:探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白(TPEF)、信号转导子和转录激活子1(STAT1)在大肠癌中的表达水平及其意义。方法:采用SYBR Green I荧光定量RT-PCR技术对50例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA及STAT1 mRNA的表达进行检测,并分析其表达情况与临床病理资料的相关性。结果:癌组织中TPEFmRNA及STAT1 mRNA的相对表达水平明显低于相应正常组织(P<0.05);肿瘤TNM分期中T3/T4癌组织表达水平明显低于T1/T2癌组织(P<0.05);合并淋巴结转移的组织中表达水平亦低于无淋巴结转移者(P<0.05);TPEFmRNA与STAT1mRNA的表达水平呈正相关(r_(癌组织)=0.537,r_(正常组织)=0.851,P<0.001)。结论:TPEF、STAT1基因在大肠癌组织中低表达,与大肠癌的转移相关,可作为大肠癌恶性潜能的检测指标之一。
基金Supported by the National Natural Science Foundation of China (Grant Nos. 50532030, 20771001 & 50703001)Team for Scientific Innovation of Anhui Province (Grant No. 2006KJ007TD)
文摘Two carbazole-based polymerization initiators possessing blue fluorescence emission have been synthesized via Wittig reaction in the solid phase at room temperature.Two-photon excited fluorescence(TPEF) spectra for them were investigated under 800 nm fs laser pulse and two-photon absorption cross sections were determined by the Z-scan technique.Then two-photon initiating polymerization(TPIP) microfabrication experiments were successfully carried out.Three-dimensional lattice and artificial defects were gained,indicating that they were viable candidates for the two-photon polymerization initiator in practical application of microfabrication.