Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncodin...Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1(WEE2-AS1)is upregulated in glioma tissues compared to normal brain tissues,and validation with quantitative real-time polymerase chain reaction(qRT–PCR)revealed that WEE2-AS1 expression was consistent with the database prediction.Fluorescence in situ hybridization(FISH)assays revealed that WEE2-AS1 was localized primarily in the cytoplasm.Clone formation experiment and EDU assay were used to detect cell proliferation ability,and Transwell assay was used to detect cell migration and invasion ability,Western-blot assay and immunofluorescence were used to determine TPM3 protein level.Functional experiments revealed that the downregulation of WEE2-AS1 impeded cell proliferation,migration,and invasion in glioma cell lines.Furthermore,downregulation of WEE2-AS1 suppressed tumor growth in vivo.Bioinformatics predictions and integrated experiments indicated that WEE2-AS1 promoted tropomyosin 3(TPM3)expression by sponging miR-29b-2-5p.A dual-luciferase reporter assay was conducted to uncover the binding of WEE2-AS1 and miR-29b-2-5p and that of miR-29b-2-5p and TPM3.Additionally,a series of rescue assays showed that WEE2-AS1 promotes proliferation,migration,and invasion by targeting miR-29b-2-5p to regulate TPM3 expression.Ultimately,the results of this study indicate that WEE2-AS1 plays an oncogenic role in glioma and may promote further investigations of the diagnostic and prognostic value of WEE2-AS1 in glioma.展开更多
According to existing reports,mutations in the slow tropomyosin gene(TPM3)may lead to congenital fiber-type disproportion(CFTD),nemaline myopathy(NM)and cap myopathy(CD).They are all congenital myopathies and are asso...According to existing reports,mutations in the slow tropomyosin gene(TPM3)may lead to congenital fiber-type disproportion(CFTD),nemaline myopathy(NM)and cap myopathy(CD).They are all congenital myopathies and are associated with clinical,pathological and ge-netic heterogeneity.A ten-year-old girl with scoliosis was unable to wean from mechanical ventilation after total intravenous anesthesia.The girl has scoliosis,respiratory insufficiency,motion delay and muscle weakness;her younger brother has a similar physiology but does not have scoliosis or respiratory insufficiency,and her parents are healthy.We conducted genetic testing and found a c.502C>G(p.R168G)heterozygous mutation in the family.This mutation originated from the father and was autosomal dominant.Muscle biopsy results indicated that no special structures were present,and the type I fiber ratio was not notably high compared to previous reports.Although the family members have the same mutations,their clinical mani-festations are quite different.展开更多
Three-degree of freedom（3-DOF） translational parallel manipulators（TPMs） have been widely studied both in industry and academia in the past decades. However, most architectures of 3-DOF TPMs are created mainly on ...Three-degree of freedom（3-DOF） translational parallel manipulators（TPMs） have been widely studied both in industry and academia in the past decades. However, most architectures of 3-DOF TPMs are created mainly on designers＇ intuition, empirical knowledge, or associative reasoning and the topology synthesis researches of 3-DOF TPMs are still limited. In order to find out the atlas of designs for 3-DOF TPMs, a topology search is presented for enumeration of 3-DOF TPMs whose limbs can be modeled as 5-DOF serial chains. The proposed topology search of 3-DOF TPMs is aimed to overcome the sensitivities of the design solution of a 3-DOF TPM for a LARM leg mechanism in a biped robot. The topology search, which is based on the concept of generation and specialization in graph theory, is reported as a step-by-step procedure with desired specifications, principle and rules of generalization, design requirements and constraints, and algorithm of number synthesis. In order to obtain new feasible designs for a chosen example and to limit the search domain under general considerations, one topological generalized kinematic chain is chosen to be specialized. An atlas of new feasible designs is obtained and analyzed for a specific solution as leg mechanisms. The proposed methodology provides a topology search for 3-DOF TPMs for leg mechanisms, but it can be also expanded for other applications and tasks.展开更多
基金supported by Jiangsu Commission of Health(M2020046).
文摘Glioma is a general malignant tumor with a dismal prognosis.Long noncoding RNAs(lncRNAs)have been implicated in the initiation and processes of tumors.An investigation of the GEPIA database revealed that long noncoding RNA WEE2 antisense RNA 1(WEE2-AS1)is upregulated in glioma tissues compared to normal brain tissues,and validation with quantitative real-time polymerase chain reaction(qRT–PCR)revealed that WEE2-AS1 expression was consistent with the database prediction.Fluorescence in situ hybridization(FISH)assays revealed that WEE2-AS1 was localized primarily in the cytoplasm.Clone formation experiment and EDU assay were used to detect cell proliferation ability,and Transwell assay was used to detect cell migration and invasion ability,Western-blot assay and immunofluorescence were used to determine TPM3 protein level.Functional experiments revealed that the downregulation of WEE2-AS1 impeded cell proliferation,migration,and invasion in glioma cell lines.Furthermore,downregulation of WEE2-AS1 suppressed tumor growth in vivo.Bioinformatics predictions and integrated experiments indicated that WEE2-AS1 promoted tropomyosin 3(TPM3)expression by sponging miR-29b-2-5p.A dual-luciferase reporter assay was conducted to uncover the binding of WEE2-AS1 and miR-29b-2-5p and that of miR-29b-2-5p and TPM3.Additionally,a series of rescue assays showed that WEE2-AS1 promotes proliferation,migration,and invasion by targeting miR-29b-2-5p to regulate TPM3 expression.Ultimately,the results of this study indicate that WEE2-AS1 plays an oncogenic role in glioma and may promote further investigations of the diagnostic and prognostic value of WEE2-AS1 in glioma.
基金This study was supported by grants from the Key Program of Chongqing Health and Family Planning Commission(grant number[2013]39:2013-1-029).
文摘According to existing reports,mutations in the slow tropomyosin gene(TPM3)may lead to congenital fiber-type disproportion(CFTD),nemaline myopathy(NM)and cap myopathy(CD).They are all congenital myopathies and are associated with clinical,pathological and ge-netic heterogeneity.A ten-year-old girl with scoliosis was unable to wean from mechanical ventilation after total intravenous anesthesia.The girl has scoliosis,respiratory insufficiency,motion delay and muscle weakness;her younger brother has a similar physiology but does not have scoliosis or respiratory insufficiency,and her parents are healthy.We conducted genetic testing and found a c.502C>G(p.R168G)heterozygous mutation in the family.This mutation originated from the father and was autosomal dominant.Muscle biopsy results indicated that no special structures were present,and the type I fiber ratio was not notably high compared to previous reports.Although the family members have the same mutations,their clinical mani-festations are quite different.
基金supported by the Chinese Scholarship Council(CSC)for his Ph D study and research at LARM in the University of Cassino and South Latium,Italy,during 2013-2015
文摘Three-degree of freedom（3-DOF） translational parallel manipulators（TPMs） have been widely studied both in industry and academia in the past decades. However, most architectures of 3-DOF TPMs are created mainly on designers＇ intuition, empirical knowledge, or associative reasoning and the topology synthesis researches of 3-DOF TPMs are still limited. In order to find out the atlas of designs for 3-DOF TPMs, a topology search is presented for enumeration of 3-DOF TPMs whose limbs can be modeled as 5-DOF serial chains. The proposed topology search of 3-DOF TPMs is aimed to overcome the sensitivities of the design solution of a 3-DOF TPM for a LARM leg mechanism in a biped robot. The topology search, which is based on the concept of generation and specialization in graph theory, is reported as a step-by-step procedure with desired specifications, principle and rules of generalization, design requirements and constraints, and algorithm of number synthesis. In order to obtain new feasible designs for a chosen example and to limit the search domain under general considerations, one topological generalized kinematic chain is chosen to be specialized. An atlas of new feasible designs is obtained and analyzed for a specific solution as leg mechanisms. The proposed methodology provides a topology search for 3-DOF TPMs for leg mechanisms, but it can be also expanded for other applications and tasks.
