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适冷菌Pseudomonas extremaustralis PF中海藻糖的合成途径及TreS基因的克隆 被引量:6
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作者 龙凤 李洁 +4 位作者 王光鹏 陈熙明 陈书燕 盛红梅 安黎哲 《兰州大学学报(自然科学版)》 CAS CSCD 北大核心 2009年第6期87-93,共7页
通过对一株高寒冰缘植物内生适冷假单胞菌Pseudomonas extremaustralis PF的研究,发现该菌中同时存在TPS/TPP,TreY/TreZ和TreS三种海藻糖合成途径,其中TreS途径的合成酶活性最高.对该菌的海藻糖合成酶TreS的基因进行克隆,得到一个新的T... 通过对一株高寒冰缘植物内生适冷假单胞菌Pseudomonas extremaustralis PF的研究,发现该菌中同时存在TPS/TPP,TreY/TreZ和TreS三种海藻糖合成途径,其中TreS途径的合成酶活性最高.对该菌的海藻糖合成酶TreS的基因进行克隆,得到一个新的TreS基因PFTreS,该基因与已报道的细菌TreS基因在核酸序列上表现出较高的同源性(最高达80.2%).根据基因序列预测的PFTreS氨基酸序列具有TreS酶的催化功能保守区,与假单胞菌P.Fluorescens Pf-5的TreS有很高的同源性.这些结果表明了Pseudomonas extremaustralis中海藻糖的合成特性,为进一步揭示海藻糖的合成与Pseudomonas extremaustralis PF的低温响应机制的关系奠定了基础. 展开更多
关键词 PSEUDOMONAS extremaustralis PF 海藻糖 tres基因 克隆
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Fibria将在其Tres Lagoas基地投建自动化桉木育苗苗圃
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作者 石瑜 《造纸信息》 2016年第6期88-88,共1页
Fibria近日宣布,将在其位于南马托格罗索州(Mato Grosso do Sul)的Tres Lagoas基地投建1个新的苗圃。该苗圃将引进荷兰最新一代技术,整个桉木树苗培育流程将采用全自动化控制,这在行业内是前所未有的。据介绍,Fibria将成为第一家采用... Fibria近日宣布,将在其位于南马托格罗索州(Mato Grosso do Sul)的Tres Lagoas基地投建1个新的苗圃。该苗圃将引进荷兰最新一代技术,整个桉木树苗培育流程将采用全自动化控制,这在行业内是前所未有的。据介绍,Fibria将成为第一家采用机器人种植桉木树苗的制浆企业。树苗种植是整个工艺流程的第一步,以往这一步需要依靠人工完成。 展开更多
关键词 Fibria tres Lagoas基地 自动化桉木育苗苗圃 荷兰最新一代技术
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水生栖热菌FL-03海藻糖合酶基因的克隆及真核表达 被引量:6
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作者 刘俊梅 聂海彦 +1 位作者 郑微微 胡耀辉 《食品科学》 EI CAS CSCD 北大核心 2010年第23期267-270,共4页
通过基因工程技术合成海藻糖合酶Tres全基因,插入到毕赤酵母菌表达载体pPICZα中。电转化毕赤酵母菌GS115后,经Zeocin抗性筛选阳性菌株。用PCR和全基因测序鉴定目的基因的表达。通过诱导表达目的蛋白,并以SDS-PAGE和Western blotting进... 通过基因工程技术合成海藻糖合酶Tres全基因,插入到毕赤酵母菌表达载体pPICZα中。电转化毕赤酵母菌GS115后,经Zeocin抗性筛选阳性菌株。用PCR和全基因测序鉴定目的基因的表达。通过诱导表达目的蛋白,并以SDS-PAGE和Western blotting进行鉴定。成功地构建了pPICZα-Tres重组质粒,并证明目的基因已整合于毕赤酵母菌的基因组。 展开更多
关键词 海藻糖合酶 tres全基因 基因克隆 真核表达
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海藻糖合成酶产生菌筛选、鉴定及其产酶特性 被引量:6
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作者 刘德海 权淑静 +4 位作者 解复红 马焕 巩涛 贾彬 陈国参 《中国酿造》 CAS 北大核心 2016年第9期95-100,共6页
以麦芽糖为唯一碳源高盐培养基,经高温培养,从温泉水样及其附近土壤中筛选得到一株菌株T2,经过生物合成途径初步验证,该菌株产海藻糖合酶,能够通过海藻糖合成酶(TreS)途径将麦芽糖转化为海藻糖。菌株T2为革兰氏阳性菌,杆状,有... 以麦芽糖为唯一碳源高盐培养基,经高温培养,从温泉水样及其附近土壤中筛选得到一株菌株T2,经过生物合成途径初步验证,该菌株产海藻糖合酶,能够通过海藻糖合成酶(TreS)途径将麦芽糖转化为海藻糖。菌株T2为革兰氏阳性菌,杆状,有芽孢,经过生物学鉴定,将其初步鉴定为芽孢杆菌属(Bacillus sp.)。对其产海藻糖合酶的酶学性质进行了研究:酶反应最适作用温度为60℃,在60℃条件下保温100min仍能保持酶活性80.7%;最适作用pH值为7.0,在pH6.0~7.5范围内稳定。采用正交试验对其发酵培养基配方进行了优化研究,确定了最佳的培养基组成为牛肉膏3.0g/L,麦芽糖20.0g/L,蛋白胨7.5g/L,无机盐(K2HPO4+NaH2PO4+MgSO4·7H2O)3.0g/L。在此条件下,菌株T2产海藻糖合酶酶活力达到310.6U/L。 展开更多
关键词 海藻糖 海藻糖合酶 tres途径 筛选 鉴定
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红色亚栖热菌海藻糖合酶在大肠杆菌中的表达及其酶学性质分析 被引量:1
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作者 王文文 张峻 +5 位作者 王宇凡 朱玥明 张娟 刘艳超 邢来君 李明春 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2011年第3期90-97,共8页
利用前期克隆得到的红色亚栖热菌CBS-01(Meiothermus ruber CBS-01)的海藻糖合酶基因(TreS),构建天然蛋白异源宿主表达载体并在大肠杆菌中获得较高的表达量.经纯化后测定该酶在pH 6.5,反应温度为50℃时活性最高.酶学性质的研究表明,Tre... 利用前期克隆得到的红色亚栖热菌CBS-01(Meiothermus ruber CBS-01)的海藻糖合酶基因(TreS),构建天然蛋白异源宿主表达载体并在大肠杆菌中获得较高的表达量.经纯化后测定该酶在pH 6.5,反应温度为50℃时活性最高.酶学性质的研究表明,TreS的最适底物为麦芽糖,其对麦芽糖的转化效率约是海藻糖的2.5倍;该酶在30~60℃、pH 3.5~9.0范围内都保留较高的酶活;2 mmol/L的一价阳离子Na^+、K^+对TreS有激活作用;低温有利于反应向海藻糖方向进行,并能减少葡萄糖副产物的生成;20℃时,几乎没有葡萄糖生成,麦芽糖至海藻糖的转化率可达65%.为工业化大规模生产海藻糖奠定了理论基础. 展开更多
关键词 红色亚栖热菌 海藻糖合酶(tres) 表达 酶学性质
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Phylogeography and morphometric variation in the Cinnamon Hummingbird complex:Amazilia rutila(Aves:Trochilidae)
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作者 Melisa Vázquez-López Nandadevi Córtes-Rodríguez +4 位作者 Sahid M.Robles-Bello Alfredo Bueno-Hernández Luz E.Zamudio-Beltrán Kristen Ruegg Blanca E.Hernández-Baños 《Avian Research》 SCIE CSCD 2021年第4期509-525,共17页
Background:The Mesoamerican dominion is a biogeographic area of great interest due to its complex topography and distinctive climatic history.This area has a large diversity of habitats,including tropical deciduous fo... Background:The Mesoamerican dominion is a biogeographic area of great interest due to its complex topography and distinctive climatic history.This area has a large diversity of habitats,including tropical deciduous forests,which house a large number of endemic species.Here,we assess phylogeographic pattern,genetic and morphometric variation in the Cinnamon Hummingbird complex Amazilia rutila,which prefers habitats in this region.This resident species is distributed along the Pacific coast from Sinaloa—including the Tres Marías Islands in Mexico to Costa Rica,and from the coastal plain of the Yucatán Peninsula of Mexico south to Belize.Methods:We obtained genetic data from 85 samples of A.rutila,using 4 different molecular markers(mtDNA:ND2,COI;nDNA:ODC,MUSK)on which we performed analyses of population structure(median-joining network,STRU CTU RE,FST,AMOVA),Bayesian and Maximum Likelihood phylogenetic analyses,and divergence time estimates.In order to evaluate the historic suitability of environmental conditions,we constructed projection models using past scenarios(Pleistocene periods),and conducted Bayesian Skyline Plots(BSP)to visualize changes in population sizes over time.To analyze morphometric variation,we took measurements of 5 morphological traits from 210 study skins.We tested for differences between sexes,differences among geographic groups(defined based on genetic results),and used PCA to examine the variation in multivariate space.Results:Using mtDNA,we recovered four main geographic groups:the Pacific coast,the Tres Marías Islands,the Chiapas region,and the Yucatán Peninsula together with Central America.These same groups were recovered by the phylogenetic results based on the multilocus dataset.Demography based on BSP results showed constant population size over time throughout the A.rutila complex and within each geographic group.Ecological niche model projec-tions onto past scenarios revealed no drastic changes in suitable conditions,but revealed some possible refuges.Mor-phometric results showed minor sexual dimorphism in this species and statistically significant differences between geographic groups.The Tres Marías Islands population was the most differentiated,having larger body size than the remaining groups.Conclusions:The best supported evolutionary hypothesis of diversification within this group corresponds to geo-graphic isolation(limited gene flow),differences in current environmental conditions,and historical habitat fragmen-tation promoted by past events(Pleistocene refugia).Four well-defined clades comprise the A.rutila complex,and we assess the importance of a taxonomic reevaluation.Our data suggest that both of A.r.graysoni(Tres Marías Islands)and A.r.rutila(Pacific coast)should be considered full species.The other two strongly supported clades are:(a)the Chiapas group(southern Mexico),and(b)the populations from Yucatán Peninsula and Central America.These clades belong to the corallirostris taxon,which needs to be split and properly named. 展开更多
关键词 Amazilia rutila Genetic structure Genetic variation PHYLOGEOGRAPHY tres Marías Islands TROCHILIDAE Tropical deciduous forest
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Redefinition of the Terminus of the Middle America Trench
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作者 Román Alvarez 《International Journal of Geosciences》 2013年第4期766-776,共11页
The terminus of the Middle America Trench has been traditionally represented as an arc, concave towards the continent. Tres Marías Islands are located at the terminus of the Middle America Trench in western Mexic... The terminus of the Middle America Trench has been traditionally represented as an arc, concave towards the continent. Tres Marías Islands are located at the terminus of the Middle America Trench in western Mexico, and their location is not only intriguing but also a key to the re-construction of the position of Baja California peninsula before separation from the North America plate. Previous re-constructions suggested various places around the location of Tres Marías Islands for the position of the tip of the peninsula, and several converge to a position that invades the area occupied by the islands, offering no explanation for the overlap. Before peninsular separation from North America, the Guadalupe trench followed a smooth curve;recreating the position of this paleo-trench yields a baseline for fixing the position of the peninsula, as well as the original position of the Tres Marías Islands fragment. A new tectonic view of the structure of the Middle America Trench terminus is proposed, replacing the traditional arc representation with a series of en echelon blocks, the northernmost terminates at the Tres Marías Escarpment. The long sides of the blocks correspond to previously identified geological faults in the Bahía de Banderas region, while their offshore continuation is supported by topographic observations. As a test of this model I show the corresponding re-construction of the position of Baja California prior to separation from the North America plate and the positional evolution of the peninsula and the Tres Marías fragment from Chron 4n.2 (7.90 Ma) to Chron 3n.4 (5.12 Ma). 展开更多
关键词 Middle AMERICA TRENCH tres Marías ISLANDS Guadalupe TRENCH MAT TERMINUS Banderas Fault
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高速卫星调制解调器
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《电子产品世界》 1998年第11期88-88,共1页
关键词 美国休斯网络系统公司 tres 卫星调制解调器 功能
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Rain-Flow Modelling Using the GR4J Model for Flood Risk Management in the Oti Watershed (Togo)
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作者 Koungbanane Dambré Kodja Japhet Domiho +2 位作者 Lemou Faya Totin Vodounon Henri Sourou Amoussou Ernest 《Open Journal of Modern Hydrology》 CAS 2024年第4期213-230,共18页
In recent years, West Africa has been confronted with hydro-climatic disasters causing crises in both urban and rural areas. The tragedy in the occurrence of such events lies in the recurrent aspect of high water and ... In recent years, West Africa has been confronted with hydro-climatic disasters causing crises in both urban and rural areas. The tragedy in the occurrence of such events lies in the recurrent aspect of high water and associated floods. The devastating floods observed in Africa’s major rivers have revealed the need to understand the causes of these phenomena and to predict their behavior in order to improve the safety of exposed people and property. The aim of this study is to reproduce flood flows using the GR4J (Rural Engineering Four Daily Parameters) model to analyze flood risk in the Oti watershed in Togo. Daily data on flows (m3/s), potential evapotranspiration (mm/day) and average precipitation (mm) over the basin from 1961-2022 collected at the National Meteorological Agency of Togo (ANAMET) and the Department of Water Resources in Lome, were used with the R software package airGR. The Data from the West African Cordex program from 1961-2100 were used to analyze projected flows. The results obtained show the GR4J model’s effectiveness in reproducing flood flows, indicating that observed flows are well simulated during the calibration and validation periods, with KGE values ranging from 0.73 to 0.85 at calibration and 0.62 to 0.81 at validation. These KGE values reflect the good performance of the GR4J model in simulating flood flows in the watershed. However, a deterioration in the KGE value was observed over the second validation period. Under these conditions, there may be false or missed alerts for flood prediction, and the use of this model should be treated with the utmost caution for decision-support purposes. 展开更多
关键词 Modeling Génie Rural à 4 Paramètres Journaliers (GR4J) Floods Kling-Gupta Efficiency (KGE) Oti Watershed Risk
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