目的探讨辣椒素(capsaicin,CAP)对水浸-束缚应激(water immersion restraint stress,WIRS)大鼠胃动力的作用及机制。方法雄性SD大鼠30只,随机分为3组:正常对照组、WIRS模型组、CAP干预组。其中正常对照组自由摄食进水;WIRS模型组每日WIR...目的探讨辣椒素(capsaicin,CAP)对水浸-束缚应激(water immersion restraint stress,WIRS)大鼠胃动力的作用及机制。方法雄性SD大鼠30只,随机分为3组:正常对照组、WIRS模型组、CAP干预组。其中正常对照组自由摄食进水;WIRS模型组每日WIRS 1 h后自由摄食进水;CAP干预组每日WIRS 1 h后喂食CAP饲料。4周后所有大鼠给予酚红溶液灌胃后麻醉处死,测定胃酚红排空率;采用ELISA法检测血浆胃动素(motilin,MTL)水平;采用免疫组化方法检测胃窦辣椒素受体(transient receptor potential vanilloid1,TRPV1)、P物质(substance P,SP)的表达;用RT-PCR方法检测c-kit m RNA、SCF m RNA的转录水平。结果大鼠胃酚红排空率:WIRS模型组胃酚红排空率显著低于其余两组(P<0.05);正常对照组胃酚红排空率与CAP干预组相比,差异无统计学意义(P>0.05)。血浆MTL水平:WIRS模型组血浆MTL水平显著低于其余两组(P<0.05);正常对照组血浆MTL水平显著低于CAP干预组(P<0.05)。免疫组化结果:大鼠胃窦组织TRPV1表达积分:CAP干预组TRPV1表达水平显著高于其余两组(P<0.05);正常对照组TRPV1表达水平与WIRS模型组相比,差异无统计学意义(P>0.05)。大鼠胃窦组织SP表达积分:WIRS模型组SP表达水平显著低于其余两组(P<0.05);正常对照组SP表达水平显著低于CAP干预组(P<0.05)。RT-PCR方法检测结果:大鼠胃窦组织c-kit m RNA表达结果:WIRS模型组c-kit m RNA转录水平显著低于正常对照组(P<0.05);正常对照组、WIRS模型组c-kit m RNA转录水平与CAP干预组相比,差异无统计学意义(P>0.05)。大鼠胃窦组织SCF m RNA表达结果:WIRS模型组SCF m RNA转录水平显著低于其余两组(P<0.05);正常对照组SCF m RNA转录水平显著低于CAP干预组(P<0.05)。结论 CAP可能对WIRS大鼠胃动力具有改善作用,其机制可能与CAP调节TRPV1、SP的表达及MTL的释放有关,是否与Cajal间质细胞相关尚不明确。展开更多
Introduction: The existence of ovarian intrinsic neurons is well established. However, the morphology and chemical phenotype are not completely characterized and are even unknown for some species used in medical resea...Introduction: The existence of ovarian intrinsic neurons is well established. However, the morphology and chemical phenotype are not completely characterized and are even unknown for some species used in medical research. The purpose of this work was to determine the morphology and chemical phenotype of intrinsic neurons of the guinea pig ovary at two ages: neonates (0 days old) and sexually mature reproductive animals (90 days old). Materials and Methods: For the morphological analysis, we employed the modified Golgi-Cox impregnation technique. For the chemical phenotype, we used immunohistochemistry and the following antibodies;tyrosine hydroxylase (TH), calcitonin gene-related peptide (CGRP), transient receptor potential type 1 (TRPV1), neuron-specific nuclear protein (NeuN) and proto-oncogene product of the cFos gene (cFos). We also used enzyme histochemistry for NADPH-diaphorase detection. Results: The number of intrinsic neurons in the neonate ovary was low in comparison to the adult guinea pig ovary. The intrinsic neurons were located in the cortex and the ovarian medulla;some were isolated or clustered, forming ganglia, and others were interconnected and formed networks. The neurons were small, medium or large. In the cortex of neonate vs adult ovaries, the small and medium neurons comprised 23% vs 36% and 5.2% vs 11.6%, respectively. In the medulla, the percent of the same neurons was 10.1% vs 10.1% and 1.1% vs 2.2% in the neonate and adult, respectively. In both cortex and medulla < 1% were large neurons at two ages. Also, the neurons were rounded, fusiform or multipolar. In the cortex, they were 12.7% vs 20.9%, 14.9% vs 24.2% and 1.1% vs 3.0%, respectively. In the medulla, the percent of small vs medium neurons was 6% vs 7.1% and 4.1% vs 4.8% in the neonate and adult ovary, respectively, and <1% were large neurons at both ages. The chemical phenotypes were in the neonate and adult: TH/NeuN-positive neurons, 16.3% vs 26.5%;CGRP/NeuN, 13.5% vs 35.8%;TRPV1/NeuN, 10.2% vs 38.6%;and cFos/NeuN, 4.6% vs 5.4%, respectively.The percent of NADPHd-positive cells in the cortex was 9.5% vs 25.1% and 3.2% vs 62.2% in the medulla in the neonate and adult, respectively. Conclusion: Altogether, these data showed that the number of ovarian intrinsic neurons was low at birth and increased in the sexually mature reproductive guinea pig. The chemical phenotype was rich and peptidergic, catecholaminergic and nitrergic in nature and positive for cFos immunoreactivity. Therefore, intrinsic neurons can be chemical sensors inside of the gonad and transmit signal to the central nervous system.展开更多
文摘目的探讨辣椒素(capsaicin,CAP)对水浸-束缚应激(water immersion restraint stress,WIRS)大鼠胃动力的作用及机制。方法雄性SD大鼠30只,随机分为3组:正常对照组、WIRS模型组、CAP干预组。其中正常对照组自由摄食进水;WIRS模型组每日WIRS 1 h后自由摄食进水;CAP干预组每日WIRS 1 h后喂食CAP饲料。4周后所有大鼠给予酚红溶液灌胃后麻醉处死,测定胃酚红排空率;采用ELISA法检测血浆胃动素(motilin,MTL)水平;采用免疫组化方法检测胃窦辣椒素受体(transient receptor potential vanilloid1,TRPV1)、P物质(substance P,SP)的表达;用RT-PCR方法检测c-kit m RNA、SCF m RNA的转录水平。结果大鼠胃酚红排空率:WIRS模型组胃酚红排空率显著低于其余两组(P<0.05);正常对照组胃酚红排空率与CAP干预组相比,差异无统计学意义(P>0.05)。血浆MTL水平:WIRS模型组血浆MTL水平显著低于其余两组(P<0.05);正常对照组血浆MTL水平显著低于CAP干预组(P<0.05)。免疫组化结果:大鼠胃窦组织TRPV1表达积分:CAP干预组TRPV1表达水平显著高于其余两组(P<0.05);正常对照组TRPV1表达水平与WIRS模型组相比,差异无统计学意义(P>0.05)。大鼠胃窦组织SP表达积分:WIRS模型组SP表达水平显著低于其余两组(P<0.05);正常对照组SP表达水平显著低于CAP干预组(P<0.05)。RT-PCR方法检测结果:大鼠胃窦组织c-kit m RNA表达结果:WIRS模型组c-kit m RNA转录水平显著低于正常对照组(P<0.05);正常对照组、WIRS模型组c-kit m RNA转录水平与CAP干预组相比,差异无统计学意义(P>0.05)。大鼠胃窦组织SCF m RNA表达结果:WIRS模型组SCF m RNA转录水平显著低于其余两组(P<0.05);正常对照组SCF m RNA转录水平显著低于CAP干预组(P<0.05)。结论 CAP可能对WIRS大鼠胃动力具有改善作用,其机制可能与CAP调节TRPV1、SP的表达及MTL的释放有关,是否与Cajal间质细胞相关尚不明确。
文摘Introduction: The existence of ovarian intrinsic neurons is well established. However, the morphology and chemical phenotype are not completely characterized and are even unknown for some species used in medical research. The purpose of this work was to determine the morphology and chemical phenotype of intrinsic neurons of the guinea pig ovary at two ages: neonates (0 days old) and sexually mature reproductive animals (90 days old). Materials and Methods: For the morphological analysis, we employed the modified Golgi-Cox impregnation technique. For the chemical phenotype, we used immunohistochemistry and the following antibodies;tyrosine hydroxylase (TH), calcitonin gene-related peptide (CGRP), transient receptor potential type 1 (TRPV1), neuron-specific nuclear protein (NeuN) and proto-oncogene product of the cFos gene (cFos). We also used enzyme histochemistry for NADPH-diaphorase detection. Results: The number of intrinsic neurons in the neonate ovary was low in comparison to the adult guinea pig ovary. The intrinsic neurons were located in the cortex and the ovarian medulla;some were isolated or clustered, forming ganglia, and others were interconnected and formed networks. The neurons were small, medium or large. In the cortex of neonate vs adult ovaries, the small and medium neurons comprised 23% vs 36% and 5.2% vs 11.6%, respectively. In the medulla, the percent of the same neurons was 10.1% vs 10.1% and 1.1% vs 2.2% in the neonate and adult, respectively. In both cortex and medulla < 1% were large neurons at two ages. Also, the neurons were rounded, fusiform or multipolar. In the cortex, they were 12.7% vs 20.9%, 14.9% vs 24.2% and 1.1% vs 3.0%, respectively. In the medulla, the percent of small vs medium neurons was 6% vs 7.1% and 4.1% vs 4.8% in the neonate and adult ovary, respectively, and <1% were large neurons at both ages. The chemical phenotypes were in the neonate and adult: TH/NeuN-positive neurons, 16.3% vs 26.5%;CGRP/NeuN, 13.5% vs 35.8%;TRPV1/NeuN, 10.2% vs 38.6%;and cFos/NeuN, 4.6% vs 5.4%, respectively.The percent of NADPHd-positive cells in the cortex was 9.5% vs 25.1% and 3.2% vs 62.2% in the medulla in the neonate and adult, respectively. Conclusion: Altogether, these data showed that the number of ovarian intrinsic neurons was low at birth and increased in the sexually mature reproductive guinea pig. The chemical phenotype was rich and peptidergic, catecholaminergic and nitrergic in nature and positive for cFos immunoreactivity. Therefore, intrinsic neurons can be chemical sensors inside of the gonad and transmit signal to the central nervous system.
