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TTV阳性肝炎患者27例临床分析
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作者 谢新生 吴文仙 《浙江医学》 CAS 2003年第10期636-637,共2页
关键词 ttV阳性肝炎 tt型肝炎病毒 治疗 病原学 诊断 酶联免疫法
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荧光定量PCR检测TTV方法学建立及临床应用 被引量:2
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作者 杨建荣 陈晓东 陶志华 《华中医学杂志》 2004年第1期17-18,28,共3页
目的 建立一种灵敏度高、特异性好、操作简便的荧光实时定量PCR技术。方法 设计针对TT型肝炎病毒(TTV)基因保守序列非转录区的一对引物和一条荧光基团双标记探针。将PCR扩增所得的产物片段克隆 ,作为定量检测的标准品。对 36例丙肝患... 目的 建立一种灵敏度高、特异性好、操作简便的荧光实时定量PCR技术。方法 设计针对TT型肝炎病毒(TTV)基因保守序列非转录区的一对引物和一条荧光基团双标记探针。将PCR扩增所得的产物片段克隆 ,作为定量检测的标准品。对 36例丙肝患者及 12 3例不同血清标志物阳性的乙肝患者、16 6例健康体检者血清中TTVDNA定量检测 ,评估TTVDNA在不同人群中检出阳性率的差异显著性。结果 该方法检测TTVDNA的灵敏度高于普通套式PCR ELISA方法 ,线性范围为 10 2~ 7拷贝 /ml;批间CV为 13.2 %~ 16 .0 % ,批内CV为 6 .5 %~ 11.3%。健康体检者、丙肝患者、乙肝患者三组人群阳性检出率无显著性差异 ;且乙肝患者 (TTVDNA +)血清ALT水平与TTVDNA载量无显著相关性 (P >0 .0 5 ) ;乙肝患者血清ALT异常与正常两组TTVDNA阳性检出率无显著性差异 (P >0 .0 5 )。结论 该方法操作简单、灵敏度高、特异性好、结果数据化 ,适合于临床实验室进行临床标本的TTVDNA定量检测。 展开更多
关键词 tt型肝炎病毒 荧光实时定量聚合酶链反应 DNA诊断 开放读码框2 非转录区
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TT virus infection in patients with chronic hepatitis B and response of TTV to lamivudine 被引量:9
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作者 Javier Moreno Garcia Rafael Barcena Marugan +3 位作者 Gloria Moraleda Garcia M Luisa Mateos Lindeman Jesus Fortun Abete Santos del Campo Terron 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第6期1261-1264,共4页
AIM: To investigate the responses of TT virus (TTV) and hepatitis B virus (HBV) to a long-term lamivudine therapy.METHODS: Sixteen patients infected with both TTV and HBV were treated with lamivudine 100 mg daily for ... AIM: To investigate the responses of TT virus (TTV) and hepatitis B virus (HBV) to a long-term lamivudine therapy.METHODS: Sixteen patients infected with both TTV and HBV were treated with lamivudine 100 mg daily for 30 months. Blood samples were drawn at the beginning of the therapy and subsequently at month 3, 6, 9, 12 and 30.Serum TTV was quantified by real time PCR and serum HBV was detected by hybridization assay and nested polymerase chain reaction.RESULTS: TTV infection was detected in 100 % of HBV-infected patients. Loss of serum TTV DNA after one year of treatment occurred in 1/16 (6 %) patients. At the end of therapy, TTV DNA was positive in 94 % of them. The decline of HBV viremia was evident at 3 months after therapy and the response rate was 31%, 44 %, 63 %, 50 % and 50 %at month 3, 6, 9, 12 and 30, respectively.CONCLUSION: TTV replication is not sensitive to lamivudine and is highly prevalent in HBV-infected patients. 展开更多
关键词 Torque teno virus ADULT Aged DNA Virus Infections FEMALE Hepatitis B Chronic Humans LAMIVUDINE MALE Middle Aged PREVALENCE Reverse Transcriptase Inhibitors Virus Replication
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TT virus and hepatitis G virus infections in Korean blood donors and patients with chronic liver disease 被引量:7
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作者 Mee Juhng Jeon Jong Hee Shin +2 位作者 Soon Pal Suh Young Chai Lim Dong Wook Ryang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第4期741-744,共4页
AIM:To determine the prevalences of TTV and HGV infections among blood donors and patients with chronic liver disease in Korea,to investigate the association of TTV and HGV infections with blood transfusion,and to ass... AIM:To determine the prevalences of TTV and HGV infections among blood donors and patients with chronic liver disease in Korea,to investigate the association of TTV and HGV infections with blood transfusion,and to assess the correlation between TTV and HGV viremia and hepatic damage. METHODS:A total of 391 serum samples were examined in this study.Samples were obtained from healthy blood donors(n=110),hepatitis B surface antigen(HBsAg)-positive donors(n=112),anti-hepatitis C virus(anti-HCV)-positive donors(n=69),patients with type B chronic liver disease (n=81),and patients with type C chronic liver disease(n=19). Trv DNA was detected using the hemi-nested PCR.HGV RNA was tested using RT-PCR.A history of blood transfusion and serum levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were also determined. RESULTS:TTV DNA was detected in 8.2%of healthy blood donors,16.1%of HBsAg-positive donors,20.3%of anti- HCV-positive donors,21.0%of patients with type B chronic liver disease,and 21.1%of patients with type C chronic liver disease.HGV RNA was detected in 1.8%of healthy blood donors,1.8%of HBsAg-positive donors,17.4%of anti-HCV-positive donors,13.6%of patients with type B chronic liver disease,and 10.5%of patients with type C chronic liver disease.The prevalence of TTV and HGV infections in HBV- or HCV-positive donors and patients was significantly higher than in healthy blood donors(P<0.05), except for the detection rate of HGV in HBsAg-positive donors which was the same as for healthy donors.There was a history of transfusion in 66.7%of TTV DNA-positive patients and 76.9%of HGV RNA-positive patients(P<0.05).No significant increase in serum ALT and AST was detected in the TTV or HGV-positive donors and patients. CONCLUSION:TTV and HGV infections are more frequently found in donors and patients infected with HBV or HCV than in healthy blood donors.However,there is no significant association between TTV or HGV infections and liver injury. 展开更多
关键词 Blood Donors Blood Transfusion Chronic Disease DNA Virus Infections DNA Viral Flaviviridae Infections GB virus C purification Hepatitis B Surface Antigens Hepatitis Viral Human Korea Liver Diseases Polymerase Chain Reaction Reference Values Reverse Transcriptase Polymerase Chain Reaction Torque teno virus
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Response of TT virus to IFN plus ribavirin treatment in patients with chronic hepatitis C 被引量:1
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作者 JavierMoreno RafaelBarcena +2 位作者 SantosdelCampo GloriaMoraleda Mluisa Mateos 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第1期143-146,共4页
AIM:TT virus (TTV) is a newly described DNA virus related to postransfusion hepatitis that produces persistent viremia in the absence of clinical manifestations.PEG-IFN plus ribavirin have been useful in the treatment... AIM:TT virus (TTV) is a newly described DNA virus related to postransfusion hepatitis that produces persistent viremia in the absence of clinical manifestations.PEG-IFN plus ribavirin have been useful in the treatment of chronic hepatitis C infection.This study investigated the responses of TT virus (TTV) and hepatitis C virus (HCV) to PEG-IFN plus ribavirin therapy. METHODS:Fifteen patients infected with HCV were treated with PEG-IFN(0.5 μg/body weight/week) and ribavirin (1000 mg-1 200 mg/daily) for 48 weeks,Blood samples were drawn at the beginning and the end of the therapy.Serum TTV DNA and HCV RNA were quantified by real time PCR. RESULTS:At the beginning of treatment,TTV infection was detected in 10/15 (66.6%) of HCV-infected patients.Loss of serum TTV DNA at the end of therapy occurred in 6/10 (60%) patients.Out of these 6 patients,4 (67%) became positive for TTV DNA after 6 months of therapy.Regarding HCV viremia,11/15 (73%) patients were negative for serum HCV RNA after 48 weeks of therapy,7/11 (64%) of these cases also became negative for TTV DNA following the combined treatment.In the 3/4 (75%) patients who were positive for HCV RNA at the end of therapy,TTV DNA was detected as well.Sustained HCV response at 6 months after treatment was 53% (8/15). CONCLUSION:No TTV sustained response can be achieved in any patient after PEG-IFN plus ribavirin administration. 展开更多
关键词 Torque teno virus ADULT Antiviral Agents DOSAGE DNA Virus Infections Drug Therapy Combination Female Hepatitis C Chronic Humans INTERFERONS Male Middle Aged Research Support Non-U.S. Gov't RIBAVIRIN Treatment Outcome
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