Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the represe...Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the representative species around the hydrothermal vent in the deep sea. They have developed unique mechanisms to adapt to the harsh environment. In this study, a 1 092 bp cDNA, designed as rpgal, was first cloned and characterized from the tubeworm Ridgeia piscesae. Sequence analysis showed that RPGAL had low homology with the known galectin. And it had two homologous carbohydrate-recognition domains, which is the characteristic of the tandem-repeat type galectins. The RPGAL was successfully recombinant expressed in Escherichia coli and purified. Analysis of biological activity revealed that RPGAL was metal ion independent and it could agglutinate all the vertebrate erythrocytes tested. It was stable at 10-50℃ and pH 5-10. And the hemagglutinating activity of RPGAL was strongly inhibited by D-Lactose and lipopolysaccharide. Although RPGAL had no effect on the microorganisms tested, it showed anti-tumor activity towards HeLa cells and HT1080 cells, which was accomplished by apoptosis. The study demonstrated that RPGAL was a novel galectin and provided a potential candidate for therapy of anti-tumor.展开更多
Seven Cornulites species,including a new one—Cornulites leonei n.sp.,are described from the Upper Ordovician Portixeddu Formation(Katian,stage slices Ka2-3)of Sardinia and the Cavá(lower Katian,stage slice Ka2)a...Seven Cornulites species,including a new one—Cornulites leonei n.sp.,are described from the Upper Ordovician Portixeddu Formation(Katian,stage slices Ka2-3)of Sardinia and the Cavá(lower Katian,stage slice Ka2)and Estana(upper Katian,stage slices Ka3-4)formations of the Pyrenees.The Sardinian and Pyrenean cornulitids represent an adaptation to live in environments with high sedimentation rates and limited hard substrates availability.Their prominent annuli could have had a stabilizing function in the soft sediment that helped cornulitids to keep a favourable position in the sediment to enable suspension feeding.The known Late Ordovician cornulitid diversity in different Gondwana areas is low,usually ranging from one to three taxa,being higher(seven)in Sardinia.Like other benthic groups during the Late Ordovician,the cornulitid tubeworm faunas within the high-latitude peri-Gondwana Province indicate a certain endemism and share morphological and ecological affinities,such as a small body size and tubes with a strikingly small apical angle.Although essentially endemic,some links with cornulitids from the Late Ordovician of Scotland are revealed.展开更多
As an important part of marine hydrothermal system, hydrothermal vent faunas live in hydrothermal inorganic environment and closely interact with hydrothermal inorganic environment. Sometimes, they can participate in ...As an important part of marine hydrothermal system, hydrothermal vent faunas live in hydrothermal inorganic environment and closely interact with hydrothermal inorganic environment. Sometimes, they can participate in the mineralization process of modern hydrothermal site. Hydrothermal vent faunas, particularly vestimentiferan and polychaete tubeworms, are occasionally preserved in the geological record. Study on the early mineralization process of hydrothermal vent fauna is significant for under- standing the interaction between mineral and organism, and also the formation and preservation mechanism of geological fossil in hydrothermal environment. In this paper, the early stage of miner- alization of Vestimentiferan Ridgeia piscesae tubes collected from Juan de Fuca Ridge is studied. The results showed that a lot of filamentous microorganisms were unevenly distributed on the surface of internal wall and in the interspace of the wall of tubeworm. In some cases, microorganisms aggregated as thin layers in or on the wall of tubeworm. The surfaces of microbial cells and the products of micro- bial degradation may play an important role in the early mineralization of tubeworm. Semitransparent thin layers of organic matter containing sulfur and sulfur granules were commonly found on the wall of tubeworm with lower degree of mineralization. The degradation production of these semitransparent thin layers may accelerate the mineralization of tube wall during the early stage. EDS results showed that on the tube walls some chemical elements such as Fe, P, Ca and Si are selectively enriched from ambient hydrothermal environment. Interestingly, P, Ca and Si covary with Fe content. Because element S originated from the bio-oxidation of H2S by symbiotic microorganism in the tissue of tubeworm, it can be considered as a biomarker when studying the mineralization process of tube wall. Based on the characteristics of tubeworms with different degrees of mineralization, we suggested that the early mineralization stage of tube wall was mainly controlled by microbial-induced mineralization and the degradation process of tube wall.展开更多
基金The Major State Basic Research Development Program of China(973 Program)under contract No.2015CB755906China Ocean Mineral Resources R&D Association under contract No.DYXM-115-02-2-16
文摘Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the representative species around the hydrothermal vent in the deep sea. They have developed unique mechanisms to adapt to the harsh environment. In this study, a 1 092 bp cDNA, designed as rpgal, was first cloned and characterized from the tubeworm Ridgeia piscesae. Sequence analysis showed that RPGAL had low homology with the known galectin. And it had two homologous carbohydrate-recognition domains, which is the characteristic of the tandem-repeat type galectins. The RPGAL was successfully recombinant expressed in Escherichia coli and purified. Analysis of biological activity revealed that RPGAL was metal ion independent and it could agglutinate all the vertebrate erythrocytes tested. It was stable at 10-50℃ and pH 5-10. And the hemagglutinating activity of RPGAL was strongly inhibited by D-Lactose and lipopolysaccharide. Although RPGAL had no effect on the microorganisms tested, it showed anti-tumor activity towards HeLa cells and HT1080 cells, which was accomplished by apoptosis. The study demonstrated that RPGAL was a novel galectin and provided a potential candidate for therapy of anti-tumor.
基金The financial supported was provided by a Sepkoski Grant from the Paleontological SocietyKing Saud University,Riyadh,Saudi Arabia,Researchers Supporting Project Number RSP 2024R140Project PDI2021-125585NB-100 of the Spanish Ministry for Science Innovation (MICINN)。
文摘Seven Cornulites species,including a new one—Cornulites leonei n.sp.,are described from the Upper Ordovician Portixeddu Formation(Katian,stage slices Ka2-3)of Sardinia and the Cavá(lower Katian,stage slice Ka2)and Estana(upper Katian,stage slices Ka3-4)formations of the Pyrenees.The Sardinian and Pyrenean cornulitids represent an adaptation to live in environments with high sedimentation rates and limited hard substrates availability.Their prominent annuli could have had a stabilizing function in the soft sediment that helped cornulitids to keep a favourable position in the sediment to enable suspension feeding.The known Late Ordovician cornulitid diversity in different Gondwana areas is low,usually ranging from one to three taxa,being higher(seven)in Sardinia.Like other benthic groups during the Late Ordovician,the cornulitid tubeworm faunas within the high-latitude peri-Gondwana Province indicate a certain endemism and share morphological and ecological affinities,such as a small body size and tubes with a strikingly small apical angle.Although essentially endemic,some links with cornulitids from the Late Ordovician of Scotland are revealed.
基金the National Natural Science Foundation of China (Grant Nos. 40532011, 40403004 and 40473032)
文摘As an important part of marine hydrothermal system, hydrothermal vent faunas live in hydrothermal inorganic environment and closely interact with hydrothermal inorganic environment. Sometimes, they can participate in the mineralization process of modern hydrothermal site. Hydrothermal vent faunas, particularly vestimentiferan and polychaete tubeworms, are occasionally preserved in the geological record. Study on the early mineralization process of hydrothermal vent fauna is significant for under- standing the interaction between mineral and organism, and also the formation and preservation mechanism of geological fossil in hydrothermal environment. In this paper, the early stage of miner- alization of Vestimentiferan Ridgeia piscesae tubes collected from Juan de Fuca Ridge is studied. The results showed that a lot of filamentous microorganisms were unevenly distributed on the surface of internal wall and in the interspace of the wall of tubeworm. In some cases, microorganisms aggregated as thin layers in or on the wall of tubeworm. The surfaces of microbial cells and the products of micro- bial degradation may play an important role in the early mineralization of tubeworm. Semitransparent thin layers of organic matter containing sulfur and sulfur granules were commonly found on the wall of tubeworm with lower degree of mineralization. The degradation production of these semitransparent thin layers may accelerate the mineralization of tube wall during the early stage. EDS results showed that on the tube walls some chemical elements such as Fe, P, Ca and Si are selectively enriched from ambient hydrothermal environment. Interestingly, P, Ca and Si covary with Fe content. Because element S originated from the bio-oxidation of H2S by symbiotic microorganism in the tissue of tubeworm, it can be considered as a biomarker when studying the mineralization process of tube wall. Based on the characteristics of tubeworms with different degrees of mineralization, we suggested that the early mineralization stage of tube wall was mainly controlled by microbial-induced mineralization and the degradation process of tube wall.
