目的用缺口末端标记技术(Td-mediated dudpo-x nick end Labeling,Tunel)对大鼠睾丸生殖细胞的凋亡做形态学观察,以期提高此方法的特异性和敏感性。方法采用大黄素灌胃后天数不同大鼠睾丸生殖细胞,用HE、Tunel经典法和Tunel改良法观察...目的用缺口末端标记技术(Td-mediated dudpo-x nick end Labeling,Tunel)对大鼠睾丸生殖细胞的凋亡做形态学观察,以期提高此方法的特异性和敏感性。方法采用大黄素灌胃后天数不同大鼠睾丸生殖细胞,用HE、Tunel经典法和Tunel改良法观察不同时期细胞凋亡水平并进行比较。结果采用Tunel改良法后,凋亡阳性细胞数量明显增加且背景浅,细胞着色深,轮廓清晰完整,凋亡指数明显提高(P<0.05)。结论改进的Tunel法适合于大鼠睾丸生殖细胞凋亡的检测。展开更多
Aim: To investigate the relationship between germ cell degeneration and apoptosis in cryptorchid rats. Methods: Thirteen 21-day-old Wistar rats were made unilaterally cryptorchid by closing the left inguinal canal. At...Aim: To investigate the relationship between germ cell degeneration and apoptosis in cryptorchid rats. Methods: Thirteen 21-day-old Wistar rats were made unilaterally cryptorchid by closing the left inguinal canal. At day 30 (Group 1, n=6) and day 60 (Group 2, n=7) after operation, the testes were removed for histopathological examination. The controls (n=8) were sham operated and were sacrificed at day 60. Germ cell apoptosis was assessed by means of the TUNEL method. Results: Spermatogenesis was arrested and the testicular and seminiferous tubular diameters were significantly reduced In the unilateral undescended testes (UUTs) compared with the contralateral descended testes (CDTs) and the control rats. However, atrophic changes, pathological calcification, necrosis of seminiferous tubule, and absence or sloughing of germ cells were not found in all the animals. The spermatocytes were the main type of germ cells undergoing apoptosis in all the groups. In the UUTs, there was a significant and time-dependent increase in the mean apoptotic index. By 60 days after surgery, increased apoptosis in germ cells was also observed in the CDTs. Conclusion: Apoptosis is the predominant mechanism of germ cell death rather than atrophy and necrosis in cryptorchidism.展开更多
文摘目的用缺口末端标记技术(Td-mediated dudpo-x nick end Labeling,Tunel)对大鼠睾丸生殖细胞的凋亡做形态学观察,以期提高此方法的特异性和敏感性。方法采用大黄素灌胃后天数不同大鼠睾丸生殖细胞,用HE、Tunel经典法和Tunel改良法观察不同时期细胞凋亡水平并进行比较。结果采用Tunel改良法后,凋亡阳性细胞数量明显增加且背景浅,细胞着色深,轮廓清晰完整,凋亡指数明显提高(P<0.05)。结论改进的Tunel法适合于大鼠睾丸生殖细胞凋亡的检测。
文摘Aim: To investigate the relationship between germ cell degeneration and apoptosis in cryptorchid rats. Methods: Thirteen 21-day-old Wistar rats were made unilaterally cryptorchid by closing the left inguinal canal. At day 30 (Group 1, n=6) and day 60 (Group 2, n=7) after operation, the testes were removed for histopathological examination. The controls (n=8) were sham operated and were sacrificed at day 60. Germ cell apoptosis was assessed by means of the TUNEL method. Results: Spermatogenesis was arrested and the testicular and seminiferous tubular diameters were significantly reduced In the unilateral undescended testes (UUTs) compared with the contralateral descended testes (CDTs) and the control rats. However, atrophic changes, pathological calcification, necrosis of seminiferous tubule, and absence or sloughing of germ cells were not found in all the animals. The spermatocytes were the main type of germ cells undergoing apoptosis in all the groups. In the UUTs, there was a significant and time-dependent increase in the mean apoptotic index. By 60 days after surgery, increased apoptosis in germ cells was also observed in the CDTs. Conclusion: Apoptosis is the predominant mechanism of germ cell death rather than atrophy and necrosis in cryptorchidism.