Magnetic resonance imaging(MRI)plays an important role in precision medicine that is hampered by the lack of contrast agents with high efficiency and the ability to translate diagnostic accuracy into therapeutic inter...Magnetic resonance imaging(MRI)plays an important role in precision medicine that is hampered by the lack of contrast agents with high efficiency and the ability to translate diagnostic accuracy into therapeutic intervention.Herein,we demonstrate a DNA-based MRI probe that overcomes previous single-mode enhancement and provides a mechanism of action for aggregationinduced dual-modal MRI signal enhancement.A facile method is developed to produce aggregated T_(1)/T_(2)dual-modal NaGdF_(4):Dy@PDA-DNA(PDA=polydopamine)MRI probes.When aggregated,this probe can further amplify MRI signal intensity and exhibit improved geometrical and positional stability in vivo.The performance of the NaGdF_(4):Dy@PDA-DNA MRI probe toward MRI-guided preoperative planning and visualization-guided surgery is verified using an orthotopic tumor-bearing mouse model.The result shows that the rapid metabolism of the degraded probe leads to the mitigation of long-term toxic effects.Therefore,the developed high-performance MRI probe is of great significance for enhancing MRI diagnostic accuracy into precision medical therapeutic interventions.展开更多
目的分析术前T_(1)S与C_(2)-C_(7)前凸角的匹配性(T_(1)slope minus C_(2)-C_(7)lordosis,T_(1)S-CL)对脊髓型颈椎病(cervical spondylotic myelopathy,CSM)患者行后路单开门椎板成形手术疗效的影响。方法回顾性分析2018年3月~2020年1...目的分析术前T_(1)S与C_(2)-C_(7)前凸角的匹配性(T_(1)slope minus C_(2)-C_(7)lordosis,T_(1)S-CL)对脊髓型颈椎病(cervical spondylotic myelopathy,CSM)患者行后路单开门椎板成形手术疗效的影响。方法回顾性分析2018年3月~2020年1月在本院接受后路单开门椎板成形术治疗的72例多节段CSM患者,依据术前X线片测得的T_(1)S-CL值分为两组:T1S-CL匹配组50例,T_(1)S-CL值≤20°;T_(1)S-CL不匹配组22例,T_(1)S-CL值>20°。随访观察两组患者的疗效和颈椎矢状位参数[T_(1)斜率(T1 slope,T1S),C_(2)-C_(7)前凸角,C_(2)-C_(7)SVA值]变化情况(“△”表示变化值),进行分组比较;同时分析T1S-CL与其他颈椎矢状位参数的相关性。结果与术前相比,两组患者末次随访时的NDI指数和JOA评分均获得明显改善(P<0.05);T_(1)S-CL不匹配组患者末次随访时的C_(2)-C_(7)前凸角较之术前显著减少,C_(2)-C_(7)SVA、T_(1)S-CL值较之术前显著增加(P<0.05)。两组间比较,T_(1)S-CL不匹配组的△C_(2)-C_(7)前凸角、△C_(2)-C_(7)SVA、△T_(1)S-CL等参数的变化值显著大于T_(1)S-CL匹配组(P<0.05),颈椎后凸发生率和颈椎矢状位失衡发生率和NDI指数显著高于T_(1)S-CL匹配组(P<0.05),且T_(1)S-CL不匹配组的JOA改善率显著低于T_(1)S-CL匹配组(P<0.05)。经Pearson相关性分析,T_(1)S-CL与T_(1)S、C_(2)-C_(7)前凸角和C_(2)-C_(7)SVA值之间,均有显著的相关性(r=0.384,-0.643,0.375;P=0.008,<0.001,0.006)。结论T_(1)S-CL的匹配性可作为颈后路单开门椎板成形手术决策的影像学参数;T_(1)S-CL不匹配的患者术后的临床获益较小,且术后颈椎后凸畸形和颈椎矢状位失衡的发生率明显增加。展开更多
基金supported by the National Natural Science Foundation of China(22134006,21721003,22204161,U2241287)the Natural Science Foundation of Shandong Province(ZR2020MB063)the Program of Science and Technology Development Plan of Jilin Province(20230101039JC)。
文摘Magnetic resonance imaging(MRI)plays an important role in precision medicine that is hampered by the lack of contrast agents with high efficiency and the ability to translate diagnostic accuracy into therapeutic intervention.Herein,we demonstrate a DNA-based MRI probe that overcomes previous single-mode enhancement and provides a mechanism of action for aggregationinduced dual-modal MRI signal enhancement.A facile method is developed to produce aggregated T_(1)/T_(2)dual-modal NaGdF_(4):Dy@PDA-DNA(PDA=polydopamine)MRI probes.When aggregated,this probe can further amplify MRI signal intensity and exhibit improved geometrical and positional stability in vivo.The performance of the NaGdF_(4):Dy@PDA-DNA MRI probe toward MRI-guided preoperative planning and visualization-guided surgery is verified using an orthotopic tumor-bearing mouse model.The result shows that the rapid metabolism of the degraded probe leads to the mitigation of long-term toxic effects.Therefore,the developed high-performance MRI probe is of great significance for enhancing MRI diagnostic accuracy into precision medical therapeutic interventions.
