[Objectives]The TaMOR gene is a gene that affects the initiation and growth of the secondary roots of wheat,but the expression patterns in different parts of the wheat root system and the differences in expression in ...[Objectives]The TaMOR gene is a gene that affects the initiation and growth of the secondary roots of wheat,but the expression patterns in different parts of the wheat root system and the differences in expression in different varieties are not clear.This study aimed to investigate the expression of the TaMOR gene in the seminal roots,secondary roots and root base.[Methods]Real-time fluorescence quantitative PCR technology was used to analyze the relative expression levels of the TaMOR gene in seminal roots,secondary roots and root base of seedlings of ancient variety Monkhead and modern variety Longchun 35.[Results]There was no significant difference in the number of seminal roots between Longchun 35 and Monkhead,and the numbers of seminal roots of the two varieties did not change significantly during the three sampling periods.The number of secondary roots and shoot dry weight of Longchun 35 were significantly higher than those of Monkhead,and the number of secondary roots and shoot dry weight of both varieties increased with the sampling time point.The root dry weight of Monkhead increased with the sampling time,while Longchun 35 showed the largest value at the second time.The fluorescence quantitative PCR results showed that for 13-day seedlings,the relative expression of the TaMOR gene in root base was significantly higher than that in the seminal roots and secondary roots.There was no significant difference in the relative expression of gene TaMOR in the root system of Monkhead and Longchun 35.[Conclusions]The root allocation of gramineous crops decreases with the breeding years,and the difference in gene TaMOR expression level needs further study.展开更多
目的探讨肿瘤转移相关基因1(M TA 1)蛋白表达与乳腺癌生物学行为之间的关系。方法应用免疫组化SP法检测56例乳腺癌组织中M TA 1蛋白及雌激素受体(ER)的表达。结果56例乳腺癌组织中39例M TA 1蛋白阳性表达,阳性率为69.6%(39/56)。M TA 1...目的探讨肿瘤转移相关基因1(M TA 1)蛋白表达与乳腺癌生物学行为之间的关系。方法应用免疫组化SP法检测56例乳腺癌组织中M TA 1蛋白及雌激素受体(ER)的表达。结果56例乳腺癌组织中39例M TA 1蛋白阳性表达,阳性率为69.6%(39/56)。M TA 1高表达与乳腺癌组织学分级、临床分期和淋巴结转移有关(P<0.05),与年龄和ER状况无关(P>0.05)。结论M TA 1蛋白可作为诊断乳腺癌转移复发的参考指标,并有望成为乳腺癌基因治疗的新靶点。展开更多
基金Supported by Huanggang Normal University High-level Cultivation Project(201615703)Startup Foundation for Docotors(201600603).
文摘[Objectives]The TaMOR gene is a gene that affects the initiation and growth of the secondary roots of wheat,but the expression patterns in different parts of the wheat root system and the differences in expression in different varieties are not clear.This study aimed to investigate the expression of the TaMOR gene in the seminal roots,secondary roots and root base.[Methods]Real-time fluorescence quantitative PCR technology was used to analyze the relative expression levels of the TaMOR gene in seminal roots,secondary roots and root base of seedlings of ancient variety Monkhead and modern variety Longchun 35.[Results]There was no significant difference in the number of seminal roots between Longchun 35 and Monkhead,and the numbers of seminal roots of the two varieties did not change significantly during the three sampling periods.The number of secondary roots and shoot dry weight of Longchun 35 were significantly higher than those of Monkhead,and the number of secondary roots and shoot dry weight of both varieties increased with the sampling time point.The root dry weight of Monkhead increased with the sampling time,while Longchun 35 showed the largest value at the second time.The fluorescence quantitative PCR results showed that for 13-day seedlings,the relative expression of the TaMOR gene in root base was significantly higher than that in the seminal roots and secondary roots.There was no significant difference in the relative expression of gene TaMOR in the root system of Monkhead and Longchun 35.[Conclusions]The root allocation of gramineous crops decreases with the breeding years,and the difference in gene TaMOR expression level needs further study.
文摘目的探讨肿瘤转移相关基因1(M TA 1)蛋白表达与乳腺癌生物学行为之间的关系。方法应用免疫组化SP法检测56例乳腺癌组织中M TA 1蛋白及雌激素受体(ER)的表达。结果56例乳腺癌组织中39例M TA 1蛋白阳性表达,阳性率为69.6%(39/56)。M TA 1高表达与乳腺癌组织学分级、临床分期和淋巴结转移有关(P<0.05),与年龄和ER状况无关(P>0.05)。结论M TA 1蛋白可作为诊断乳腺癌转移复发的参考指标,并有望成为乳腺癌基因治疗的新靶点。
