In this study, we assess the effectiveness of utilizing a straight knee kick in the backstroke with regard to the overall time, max speed, and distance per stroke when using a straight knee butterfly technique. We ana...In this study, we assess the effectiveness of utilizing a straight knee kick in the backstroke with regard to the overall time, max speed, and distance per stroke when using a straight knee butterfly technique. We analyzed freestyle kick of knee angles from 7-Olympic medalists, 1970＇s S-shaped pulling pattern stroke was used with a bending knee freestyle kick. The straight knee kick used in butterfly competitions resulted in improvements from 54.74 to 51.00 in Men＇s 100-meter butterfly and fi＇om 23.43 to 23.02 in Men＇s 50-meter butterfly. Using a straight knee kick in backstroke competitions resulted in improvements from 1：01.74 to 1：00.55 in the 100-meter backstroke event. Increases in performance speed, max speed and distance per stroke among three elite level swimmers are highly correlated to duration of the kick resistance, and straight knee kick in backstroke. The straight knee kick also resulted in an increase in lactate acid.展开更多
In this study,we assess the effectiveness of utilizing a downward breaststroke kick with regard to the overall time,max speed and velocity per stroke when using a downward breaststroke kick technique.We analyzed breas...In this study,we assess the effectiveness of utilizing a downward breaststroke kick with regard to the overall time,max speed and velocity per stroke when using a downward breaststroke kick technique.We analyzed breaststroke kick of knee angles from 8 elite world class swimmers.The downward breaststroke kick used in breaststroke competitions resulted in improvements average of 100 yard or 100 meter breaststroke from 1:05.10 s to 1:01.97 s.Increases in performance max speed and velocity per stroke among 9 elite world class swimmers are highly correlated to duration of the kick aerodynamic buoyant farce in breaststroke.The downward breaststroke kick also resulted in an increase in lactate acid.展开更多
Background The study objective was to test the hypothesis that low crude protein(CP)diet with crystalline amino acids(CAA)supplementation improves Lys utilization efficiency for milk production and reduces protein tur...Background The study objective was to test the hypothesis that low crude protein(CP)diet with crystalline amino acids(CAA)supplementation improves Lys utilization efficiency for milk production and reduces protein turnover and muscle protein breakdown.Eighteen lactating multiparous Yorkshire sows were allotted to 1 of 2 isocaloric diets(10.80 MJ/kg net energy):control(CON;19.24%CP)and reduced CP with“optimal”AA profile(OPT;14.00%CP).Sow body weight and backfat were recorded on d 1 and 21 of lactation and piglets were weighed on d 1,14,18,and 21 of lactation.Between d 14 and 18,a subset of 9 sows(CON=4,OPT=5)was infused with a mixed solution of 3-[methyl-2H3]histidine(bolus injection)and[13C]bicarbonate(priming dose)first,then a constant 2-h[13C]bicarbonate infusion followed by a 6-h primed constant[1-13C]lysine infusion.Serial blood and milk sampling were performed to determine plasma and milk Lys enrichment,Lys oxidation rate,whole body protein turnover,and muscle protein breakdown.Results Over the 21-d lactation period,compared to CON,sows fed OPT had greater litter growth rate(P<0.05).Compared to CON,sows fed OPT had greater efficiency of Lys(P<0.05),Lys mammary flux(P<0.01)and whole-body protein turnover efficiency(P<0.05).Compared to CON,sows fed OPT tended to have lower whole body protein breakdown rate(P=0.069).Muscle protein breakdown rate did not differ between OPT and CON(P=0.197).Conclusion Feeding an improved AA balance diet increased efficiency of Lys and reduced whole-body protein turnover and protein breakdown.These results imply that the lower maternal N retention observed in lactating sows fed improved AA balance diets in previous studies may be a result of greater partitioning of AA towards milk rather than greater body protein breakdown.展开更多
Fatty acids are not only widely known as energy sources,but also play important roles in many metabolic pathways.The significance of fatty acids in modulating the reproductive potential of livestock has received great...Fatty acids are not only widely known as energy sources,but also play important roles in many metabolic pathways.The significance of fatty acids in modulating the reproductive potential of livestock has received greater recognition in recent years.Functional fatty acids and their metabolites improve follicular development,oocyte maturation and embryo development,as well as endometrial receptivity and placental vascular development,through enhancing energy supply and precursors for the synthesis of their productive hormones,such as steroid hormones and prosta-glandins.However,many studies are focused on the impacts of individual functional fatty acids in the reproductive cycle,lacking studies involved in deeper mechanisms and optimal fatty acid requirements for specific physiological stages.Therefore,an overall consideration of the combination and synergy of functional fatty acids and the establish-ment of optimal fatty acid requirement for specific stages is needed to improve reproductive potential in livestock.展开更多
With increasing strict regulation on single-use plastics,lactic acid(LA)and alkyl lactates,as essential monomers for bio-degradable polylactic acid(PLA)plastic products,have gained worldwide attention in both academia...With increasing strict regulation on single-use plastics,lactic acid(LA)and alkyl lactates,as essential monomers for bio-degradable polylactic acid(PLA)plastic products,have gained worldwide attention in both academia and industry.While LA is still dominantly produced through fermentation processes from start,chemical synthesis from cellulosic biomass remains a grand challenge,owing to poor selectivity in activating CAH and CAC bonds in sugar molecules.To our best knowledge,recent publications have been focused on hydrothermal conversion of glucose to LA,while this review summarizes the highlights on direct thermal conversion of fructose as starting material to LA and derivatives.In particular,the synergies of metal/metal cations and acid/base catalysts will be critically revised on retro-aldol and dehydration reactions.This work will provide insights into rational design of active and selective catalysts for the production of carboxylic acids from biomass feedstocks.展开更多
Altered blood chemistry, acid-base and electrolyte are suggested determinants of sleep disturbance, with frequent arousal at high altitude even in well and long-trained altitude marathon runners. In this sample of exp...Altered blood chemistry, acid-base and electrolyte are suggested determinants of sleep disturbance, with frequent arousal at high altitude even in well and long-trained altitude marathon runners. In this sample of experienced altitude marathon runners with maximal aerobic power at sea level of 61.4 ± 2.7 ml/kg-1·min-1 we found that pO2 and percent of oxygen saturation (%SO2) were lower at2050 mand3480 mthan at sea level;pO2 was higher after 38 - 41 hours than after 30 - 31 hours of acclimatization at3480 m(P 2 decreased (P 2 and (P 2 at a sleeping altitude of3480 mwas lower (P Simple regression analysis disclosed a significant correlation between the changes in TST and the percent of REM sleep and the changes in %SpaO2 recorded during sleep (P 2 at higher altitude and the percent of W and of TST (P 2, tCO2 and [HCO3-] correlated negatively and significantly with the percent of REM sleep changes at high altitude (P 2 and pH and correlated negatively with the changes in %SO2, pCO2, tCO2, and [HCO3-] (P ++] and [BE] and negatively with the changes in buffered bases [BB] and [BEeffective] (P 2 and significantly and negatively with the changes in [K+] (P 2, tCO2, [HCO3-] and [K+]展开更多
Objective: Abnormal metabolism is the underlying reason for breast cancer progression. Decreased lactate dehydrogenase B(LDHB) has been detected in breast cancer but the function of LDHB remains unknown.Methods: Weste...Objective: Abnormal metabolism is the underlying reason for breast cancer progression. Decreased lactate dehydrogenase B(LDHB) has been detected in breast cancer but the function of LDHB remains unknown.Methods: Western blot was used to analyze LDHB expression in breast cancer cells. The impact of LDHB on tumor cell migration and invasion was determined using Transwell assays, wound healing assays, and a mouse lung metastasis model. Subcutaneous tumor formation, a natural killer(NK) cell cytotoxicity assay, and flow cytometry evaluated NK cell activation. Immunofluorescence and quantitative real-time PCR detected NK cell activation markers. Kaplan-Meier analysis evaluated the effect of immune cell infiltration on prognosis. Single-sample gene set enrichment analysis determined NK cell activation scores. A support vector machine predicted the role of LDHB in NK cell activation.Results: In this study we showed that LDHB inhibits the breast cancer cell metastasis and orchestrates metabolic reprogramming within tumor cells. Our results revealed that LDHB-mediated lactic acid clearance in breast cancer cells triggers NK cell activation within the tumor microenvironment. Our findings, which were confirmed in a murine model, demonstrated that LDHB in tumor cells promotes NK cell activation and ultimately results in the eradication of malignant cells. Clinically, our study further validated that LDHB affects immune cell infiltration and function. Specifically, its expression has been linked to enhanced NK cell-mediated cytotoxicity and improved patient survival. Furthermore, we identified LDHB expression in tumors as an important predictor of NK cell activation, with strong predictive ability in some cancers.Conclusions: Our results suggest that LDHB is a promising target for activating the tumor immune microenvironment in breast cancer, where LDHB-associated lactic acid clearance leads to increased NK cell activity. This study highlights the critical role of LDHB in regulating immune responses and its potential as a therapeutic target for breast cancer.展开更多
文摘In this study, we assess the effectiveness of utilizing a straight knee kick in the backstroke with regard to the overall time, max speed, and distance per stroke when using a straight knee butterfly technique. We analyzed freestyle kick of knee angles from 7-Olympic medalists, 1970＇s S-shaped pulling pattern stroke was used with a bending knee freestyle kick. The straight knee kick used in butterfly competitions resulted in improvements from 54.74 to 51.00 in Men＇s 100-meter butterfly and fi＇om 23.43 to 23.02 in Men＇s 50-meter butterfly. Using a straight knee kick in backstroke competitions resulted in improvements from 1：01.74 to 1：00.55 in the 100-meter backstroke event. Increases in performance speed, max speed and distance per stroke among three elite level swimmers are highly correlated to duration of the kick resistance, and straight knee kick in backstroke. The straight knee kick also resulted in an increase in lactate acid.
文摘In this study,we assess the effectiveness of utilizing a downward breaststroke kick with regard to the overall time,max speed and velocity per stroke when using a downward breaststroke kick technique.We analyzed breaststroke kick of knee angles from 8 elite world class swimmers.The downward breaststroke kick used in breaststroke competitions resulted in improvements average of 100 yard or 100 meter breaststroke from 1:05.10 s to 1:01.97 s.Increases in performance max speed and velocity per stroke among 9 elite world class swimmers are highly correlated to duration of the kick aerodynamic buoyant farce in breaststroke.The downward breaststroke kick also resulted in an increase in lactate acid.
基金financially supported by funds from the USDA-NIFA(award number 2014-67015-21832)。
文摘Background The study objective was to test the hypothesis that low crude protein(CP)diet with crystalline amino acids(CAA)supplementation improves Lys utilization efficiency for milk production and reduces protein turnover and muscle protein breakdown.Eighteen lactating multiparous Yorkshire sows were allotted to 1 of 2 isocaloric diets(10.80 MJ/kg net energy):control(CON;19.24%CP)and reduced CP with“optimal”AA profile(OPT;14.00%CP).Sow body weight and backfat were recorded on d 1 and 21 of lactation and piglets were weighed on d 1,14,18,and 21 of lactation.Between d 14 and 18,a subset of 9 sows(CON=4,OPT=5)was infused with a mixed solution of 3-[methyl-2H3]histidine(bolus injection)and[13C]bicarbonate(priming dose)first,then a constant 2-h[13C]bicarbonate infusion followed by a 6-h primed constant[1-13C]lysine infusion.Serial blood and milk sampling were performed to determine plasma and milk Lys enrichment,Lys oxidation rate,whole body protein turnover,and muscle protein breakdown.Results Over the 21-d lactation period,compared to CON,sows fed OPT had greater litter growth rate(P<0.05).Compared to CON,sows fed OPT had greater efficiency of Lys(P<0.05),Lys mammary flux(P<0.01)and whole-body protein turnover efficiency(P<0.05).Compared to CON,sows fed OPT tended to have lower whole body protein breakdown rate(P=0.069).Muscle protein breakdown rate did not differ between OPT and CON(P=0.197).Conclusion Feeding an improved AA balance diet increased efficiency of Lys and reduced whole-body protein turnover and protein breakdown.These results imply that the lower maternal N retention observed in lactating sows fed improved AA balance diets in previous studies may be a result of greater partitioning of AA towards milk rather than greater body protein breakdown.
基金funded by National Natural Science Foundation of China(32022080).
文摘Fatty acids are not only widely known as energy sources,but also play important roles in many metabolic pathways.The significance of fatty acids in modulating the reproductive potential of livestock has received greater recognition in recent years.Functional fatty acids and their metabolites improve follicular development,oocyte maturation and embryo development,as well as endometrial receptivity and placental vascular development,through enhancing energy supply and precursors for the synthesis of their productive hormones,such as steroid hormones and prosta-glandins.However,many studies are focused on the impacts of individual functional fatty acids in the reproductive cycle,lacking studies involved in deeper mechanisms and optimal fatty acid requirements for specific physiological stages.Therefore,an overall consideration of the combination and synergy of functional fatty acids and the establish-ment of optimal fatty acid requirement for specific stages is needed to improve reproductive potential in livestock.
基金the funds from National Natural Science Foundation of China (22078365, 21706290)Natural Science Foundation of Shandong Province (ZR2017MB004)+2 种基金Innovative Research Funding from Qingdao City, Shandong Province (17-1-1-80-jch)Fundamental Research Funds for the Central Universities and Development Fund of State Key Laboratory of Heavy Oil Processing (17CX02017A, 20CX02204A)New Faculty Start-Up Funding from China University of Petroleum (YJ201601059)
文摘With increasing strict regulation on single-use plastics,lactic acid(LA)and alkyl lactates,as essential monomers for bio-degradable polylactic acid(PLA)plastic products,have gained worldwide attention in both academia and industry.While LA is still dominantly produced through fermentation processes from start,chemical synthesis from cellulosic biomass remains a grand challenge,owing to poor selectivity in activating CAH and CAC bonds in sugar molecules.To our best knowledge,recent publications have been focused on hydrothermal conversion of glucose to LA,while this review summarizes the highlights on direct thermal conversion of fructose as starting material to LA and derivatives.In particular,the synergies of metal/metal cations and acid/base catalysts will be critically revised on retro-aldol and dehydration reactions.This work will provide insights into rational design of active and selective catalysts for the production of carboxylic acids from biomass feedstocks.
文摘Altered blood chemistry, acid-base and electrolyte are suggested determinants of sleep disturbance, with frequent arousal at high altitude even in well and long-trained altitude marathon runners. In this sample of experienced altitude marathon runners with maximal aerobic power at sea level of 61.4 ± 2.7 ml/kg-1·min-1 we found that pO2 and percent of oxygen saturation (%SO2) were lower at2050 mand3480 mthan at sea level;pO2 was higher after 38 - 41 hours than after 30 - 31 hours of acclimatization at3480 m(P 2 decreased (P 2 and (P 2 at a sleeping altitude of3480 mwas lower (P Simple regression analysis disclosed a significant correlation between the changes in TST and the percent of REM sleep and the changes in %SpaO2 recorded during sleep (P 2 at higher altitude and the percent of W and of TST (P 2, tCO2 and [HCO3-] correlated negatively and significantly with the percent of REM sleep changes at high altitude (P 2 and pH and correlated negatively with the changes in %SO2, pCO2, tCO2, and [HCO3-] (P ++] and [BE] and negatively with the changes in buffered bases [BB] and [BEeffective] (P 2 and significantly and negatively with the changes in [K+] (P 2, tCO2, [HCO3-] and [K+]
基金supported by the Shenzhen Science and Technology Program (Grant no. JCYJ20230807090459001)the Joint Research Fund of the National Science Fund of China Science and Technology Development Fund of Macao SAR (No. 32161160303 for NSFC and No. 0010/2021/AFJ for FDCT)the Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University (Grant no. ZNJC202330)。
文摘Objective: Abnormal metabolism is the underlying reason for breast cancer progression. Decreased lactate dehydrogenase B(LDHB) has been detected in breast cancer but the function of LDHB remains unknown.Methods: Western blot was used to analyze LDHB expression in breast cancer cells. The impact of LDHB on tumor cell migration and invasion was determined using Transwell assays, wound healing assays, and a mouse lung metastasis model. Subcutaneous tumor formation, a natural killer(NK) cell cytotoxicity assay, and flow cytometry evaluated NK cell activation. Immunofluorescence and quantitative real-time PCR detected NK cell activation markers. Kaplan-Meier analysis evaluated the effect of immune cell infiltration on prognosis. Single-sample gene set enrichment analysis determined NK cell activation scores. A support vector machine predicted the role of LDHB in NK cell activation.Results: In this study we showed that LDHB inhibits the breast cancer cell metastasis and orchestrates metabolic reprogramming within tumor cells. Our results revealed that LDHB-mediated lactic acid clearance in breast cancer cells triggers NK cell activation within the tumor microenvironment. Our findings, which were confirmed in a murine model, demonstrated that LDHB in tumor cells promotes NK cell activation and ultimately results in the eradication of malignant cells. Clinically, our study further validated that LDHB affects immune cell infiltration and function. Specifically, its expression has been linked to enhanced NK cell-mediated cytotoxicity and improved patient survival. Furthermore, we identified LDHB expression in tumors as an important predictor of NK cell activation, with strong predictive ability in some cancers.Conclusions: Our results suggest that LDHB is a promising target for activating the tumor immune microenvironment in breast cancer, where LDHB-associated lactic acid clearance leads to increased NK cell activity. This study highlights the critical role of LDHB in regulating immune responses and its potential as a therapeutic target for breast cancer.
