With the technology of morphology, histology and nutrition, the characteristics of organ development and the source and demand of nutritional at different stages in post-embryonic （water temperature 3-14℃） of Hucho...With the technology of morphology, histology and nutrition, the characteristics of organ development and the source and demand of nutritional at different stages in post-embryonic （water temperature 3-14℃） of Hucho taimen were discussed. The results showed that the membrane of H. taimen fertilized ovum was ruptured in 30 d, the prototype of U-type stomach was formed, the mouth could open and close freely, and nutrition was supplied from its yolk sac. The membrane ruptured after 8 d, teeth formed, and anus communicated with outsiders. The oropharyngeal cavity, esophagus, U-type stomach, intestine, and anus initially formed in the digestive tube, which nutrition was supplied from the huge yolk sac. The membrane ruptured after 16 d, pancreas and pyloric caeca appeared, larvae could intake and digest exogenous food, fish mixed nutrition, which was from yolk sac and outsiders, mainly depended on endogenous. The membrane ruptured after 24 d, the fish began floating and intaking actively, the fish mixed nutrition which mainly depended on exogenous. The membrane ruptured after 30 d, the yolk sac was completely absorbed, the function and structure of each digestive organs developed consummately, fish was from mixed nutrition into the stage of exogenous nutrition, the nutritional need of juveniles was below 10% fat, level of the optimal protein was 50%.展开更多
[ Objective] The aim was to study the effects of four feeding attractants（dimethyl-15-propiothetin, trimethylamine oxide, betaine, sodium- 5＇-inosinate） on feed intake, growth performance, body composition, digesti...[ Objective] The aim was to study the effects of four feeding attractants（dimethyl-15-propiothetin, trimethylamine oxide, betaine, sodium- 5＇-inosinate） on feed intake, growth performance, body composition, digestive enzyme activities and serum indices of Hucho taimen with initial body weight 9.39 ±0.26 g. [ Method] The Hucho taimen was raised in water flow system. There was one control group and four process group. Each diet was randomly assigned to triplicates of 50 fish for 56 days. During the experiment, the water temperature fluctuated from 9.3℃ to 14.2℃ and the dissolved oxygen was above 8.0 mg/L. [ Result ] The weight gain rate and specific growth rate increased significantly by adding 0.2% dimethyl- 13-propiothetin, 0.2% trimethylamine oxide, 0.2% betaine, but there were no changes with 0.05% sodium-5＇-inosinate supplementation （ P 〉 0.05）. The body crude protein increased significantly by supplementing 0.2% dimethyl-15-propiothetin（ P 〈0.05）, and body crude lipid decreased signifi- cantly by adding 0.2% trimethylamine oxide and 0.2% betaine（ P 〈0.05）, but body composition had no changes by supplementing 0.05% sodium- 5＇-inosinate（ P 〉0.05）. The digestive enzyme activities increased significantly by adding 0.2% dimethyl-13-propiothetin and 0.2% trimethylamine oxide（ P 〈0.05）, and the lipase increased significantly by supplementing 0.2% betaine（ P 〈 0.05）, but digestive enzyme activities had no changes supplemented 0.05% sodium-5＇-inosinate（ P 〉 0.05）. The serum total protein, albumin and globulin increased significantly added 0.2% dimethyl-15- propiothetin（ P 〈0.05）, and the high density lipoprotein content increased while cholesterol, triglycedde and low density lipoprotein decreased sig- nificantly added 0.2% trimethylamine oxide and 0.2% betaine（ P 〈 0.05）, and serum complement 3 and complement 4 increased significantly sup- plemented 0.05% sodium-5＇-inosinate （ P 〈 0.05）. [ Conclusion] The food intake, growth performance, body composition and digestive ability of taimen were improved supplemented with 0.2% dimethyl-15-propiothetin, 0.2% trimethylamine oxide and 0.2% betaine, respectively（ P 〈 0.05）, but not 0.05% sodium-5＇-inosinate. The immunity of body was improved by adding 0.05% sodium-5＇-inosinate.展开更多
A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length...A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.展开更多
基金supported by the Tackle Key Problems in National Science and Technology(2006BAD03B08-01)the Heilongjiang Emphasis Technology,China(C2008-35)the Special Talents in Harbin,China(2009RFXYN044)
文摘With the technology of morphology, histology and nutrition, the characteristics of organ development and the source and demand of nutritional at different stages in post-embryonic （water temperature 3-14℃） of Hucho taimen were discussed. The results showed that the membrane of H. taimen fertilized ovum was ruptured in 30 d, the prototype of U-type stomach was formed, the mouth could open and close freely, and nutrition was supplied from its yolk sac. The membrane ruptured after 8 d, teeth formed, and anus communicated with outsiders. The oropharyngeal cavity, esophagus, U-type stomach, intestine, and anus initially formed in the digestive tube, which nutrition was supplied from the huge yolk sac. The membrane ruptured after 16 d, pancreas and pyloric caeca appeared, larvae could intake and digest exogenous food, fish mixed nutrition, which was from yolk sac and outsiders, mainly depended on endogenous. The membrane ruptured after 24 d, the fish began floating and intaking actively, the fish mixed nutrition which mainly depended on exogenous. The membrane ruptured after 30 d, the yolk sac was completely absorbed, the function and structure of each digestive organs developed consummately, fish was from mixed nutrition into the stage of exogenous nutrition, the nutritional need of juveniles was below 10% fat, level of the optimal protein was 50%.
基金funded by Special Fund for Agro-scientific Research in the Public Interest(201003055)National Key Technology Research and Development Program in the 12th Five Year Plan of China(2012BAD25B10)the Fundamental Research Funds for the Central Institutes of HRFRI(201103)
文摘[ Objective] The aim was to study the effects of four feeding attractants（dimethyl-15-propiothetin, trimethylamine oxide, betaine, sodium- 5＇-inosinate） on feed intake, growth performance, body composition, digestive enzyme activities and serum indices of Hucho taimen with initial body weight 9.39 ±0.26 g. [ Method] The Hucho taimen was raised in water flow system. There was one control group and four process group. Each diet was randomly assigned to triplicates of 50 fish for 56 days. During the experiment, the water temperature fluctuated from 9.3℃ to 14.2℃ and the dissolved oxygen was above 8.0 mg/L. [ Result ] The weight gain rate and specific growth rate increased significantly by adding 0.2% dimethyl- 13-propiothetin, 0.2% trimethylamine oxide, 0.2% betaine, but there were no changes with 0.05% sodium-5＇-inosinate supplementation （ P 〉 0.05）. The body crude protein increased significantly by supplementing 0.2% dimethyl-15-propiothetin（ P 〈0.05）, and body crude lipid decreased signifi- cantly by adding 0.2% trimethylamine oxide and 0.2% betaine（ P 〈0.05）, but body composition had no changes by supplementing 0.05% sodium- 5＇-inosinate（ P 〉0.05）. The digestive enzyme activities increased significantly by adding 0.2% dimethyl-13-propiothetin and 0.2% trimethylamine oxide（ P 〈0.05）, and the lipase increased significantly by supplementing 0.2% betaine（ P 〈 0.05）, but digestive enzyme activities had no changes supplemented 0.05% sodium-5＇-inosinate（ P 〉 0.05）. The serum total protein, albumin and globulin increased significantly added 0.2% dimethyl-15- propiothetin（ P 〈0.05）, and the high density lipoprotein content increased while cholesterol, triglycedde and low density lipoprotein decreased sig- nificantly added 0.2% trimethylamine oxide and 0.2% betaine（ P 〈 0.05）, and serum complement 3 and complement 4 increased significantly sup- plemented 0.05% sodium-5＇-inosinate （ P 〈 0.05）. [ Conclusion] The food intake, growth performance, body composition and digestive ability of taimen were improved supplemented with 0.2% dimethyl-15-propiothetin, 0.2% trimethylamine oxide and 0.2% betaine, respectively（ P 〈 0.05）, but not 0.05% sodium-5＇-inosinate. The immunity of body was improved by adding 0.05% sodium-5＇-inosinate.
基金Supported by the National Science and Technology Support Plan Project(2012BDA25B10)the Science and Technology Project of Heilongjiang Province(GZ11B201)+1 种基金the Fundamental Research Fund for Central Public Welfare Research Institutes(201109)the Open Project of Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture(2012NYBSJ-03)
文摘A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.
