Pufferfish is prone to deterioration due to abundant nutrients and high moisture content.Drying technology can extend the shelf life and enhance the flavor quality of aquatic products.The study investigated the effect...Pufferfish is prone to deterioration due to abundant nutrients and high moisture content.Drying technology can extend the shelf life and enhance the flavor quality of aquatic products.The study investigated the effect of hot air drying(HAD),microwave vacuum drying(MVD)and hot air assisted radio frequency drying(HARFD)on the taste and volatile profiles of Takifugu obscurus.Different drying methods had significant influence on the color,rehydration,5’-nucleotides,free amino acids and volatile components(P<0.05).The results showed that HAD and HARFD could promote the flavor of T.obscurus by producing higher equivalent umami concentration(EUC)values,which were about two times of MVD group,and more pronounced pleasant odor according to sensory analysis.HAD is more appropriate for industrial application than HARFD and MVD considering the economic benefits.This study could provide a reference for the industrial application of drying T.obscurus.展开更多
Objective:To assess the toxicity of the pufferfish Takifugu oblongus,from Chennai coast,Tamil Nadu,India and to detect the presence of Tetrodotoxin(TTX).Methods:The toxicity was evaluated by mouse bioassay using Swiss...Objective:To assess the toxicity of the pufferfish Takifugu oblongus,from Chennai coast,Tamil Nadu,India and to detect the presence of Tetrodotoxin(TTX).Methods:The toxicity was evaluated by mouse bioassay using Swiss Albino mice which were expressed in mouse units(MU).Gross anatomical features were observed which is followed by histopathology of the dead mice tissues to establish the toxicity.Instrumental analysis for the presence of tetrodotoxin was also performed through GC-MS and HPLC.Results:The toxicity of ovary was the maximum with 163 MU/g and lowest toxicity was observed in skin with 75.88 MU/g.Histopathological analyses of the dead mice showed various cellular degenerations and inflammations.The amount of Tetrodotoxin detected through GC-MS and HPLC was more reliable and sensitive than the customary mouse bioassay as instrumental analyses were able to detect even nanograms of the toxin.Conclusions:The present study evidently proved that Takifugu oblongus is highly toxic and consumption of the same can pose serious threat for health and possible lethality to humans.展开更多
Tiger puffer(Takifugu rubripes) is a promising species for aquaculture production because of its high value and limited supply. However, in the north of China, using sea cages to culture this species in winter is hamp...Tiger puffer(Takifugu rubripes) is a promising species for aquaculture production because of its high value and limited supply. However, in the north of China, using sea cages to culture this species in winter is hampered by the fact that the seawater temperature is extremely low. Here, a large scale commercial production of tiger puffer has been successfully realized using a recirculating aquaculture system(RAS) from 3 October 2012 to 31 May 2013. The RAS was comprised of nine culture tanks(total water volume 200 m3) and stocked with approximately a total of 14400 fish(initial mean weight 160 g). The tiger puffer was hand-fed at a rate of 0.7% of total body weight per day, and the feed conversion rate was(1.21 ± 0.3) kg kg-1. The recycle water in RAS was treated by a sieve bend screen, a foam fractionator, a submerged biofilter, an UV sterilizer and a submersible aerator. During the whole culture period, an excellent water quality control was achieved in RAS. At the end of this experiment, the survival rate of tiger puffer was more than 98%. The final tank densities averaged 31.2 kg m-3, and the final individual mean weight was 440 g.展开更多
Estradiol treatment during early life stages of tiger puffer Takifugu rubripes induces feminization in genetic males.However,the ovaries in genetic males may revert to testes once estradiol treatment is halted.Therefo...Estradiol treatment during early life stages of tiger puffer Takifugu rubripes induces feminization in genetic males.However,the ovaries in genetic males may revert to testes once estradiol treatment is halted.Therefore studies should investigate molecular mechanisms underlying ovary-to-testis recovery in genetic males after treatment.In the present study,tiger puffer were exposed to 10,and 100μg/L 17β-estradiol(E 2)from 15 to 100 days post-hatching(dph),then gonad phenotypes and expression profi les of six sex-related genes(cyp19a,foxl2,dmrt1,amh,sox9a,and sox9b)were characterized after the exposure.Results showed that both 10 and 100μg/L E2 induced ovarian development in genetic males at 100 dph.However,all ovaries induced by 10μg/L E2 first developed into intersexual gonads and subsequently reverted to testes after the exposure.As for treatment of 100μg/L E2,while the rest of the ovaries maintained morphological stability,percentages of intersexual gonads reached 38%-57%,and none were reverted to testes.Increased mRNA levels of cyp19a,foxl2 and sox9b and decreased mRNA levels of dmrt1,amh,and sox9a were observed during the ovarian development in genetic males.While contrary gene expression profiles were detected during ovary-to-testis transformation.The mRNA levels of all the six genes were increased during the development of intersexual gonads.These results indicated that up-regulation of dmrt1,amh and sox9a is associated with initial ovary-to-intersexual transformation,and suppression of foxl2,cyp19a and sox9b is essential for complete ovary-to-testis recovery in genetic males.This research will help to trace the molecular processes underlying gonadal transformation in teleosts.展开更多
This study used tiger puffer Takifugu rubripes to explore new,simple methods for lowtemperature-induced masculinization in a cultured fish without the use of ultraviolet irradiation or sex hormones.An orthogonal test ...This study used tiger puffer Takifugu rubripes to explore new,simple methods for lowtemperature-induced masculinization in a cultured fish without the use of ultraviolet irradiation or sex hormones.An orthogonal test L9(3^4)design was used to consider three factors at three levels:treatment starting times(days post-hatch,dph:factor A)of 20,50 and 80 dph;treatment temperatures(factor B)of 13℃,15℃and 17℃;and treatment durations(factor C)of 30,45 and 60 days.A control group was reared at 21±1℃.The experiments were repeated twice.At 230 dph,the gonads were removed from thirty randomly sampled fish in each group.Histological observations and analysis of single nucleotide polymorphisms(SNP)were used to identified pseudo males,which biological sex was male and genetic sex were female(XX).Treatment group 4(A2B1C2)resulted in the highest proportion of males(75%).According to the intuitive analysis of the orthogonal-array experiments,the optimal combination of lowtemperature-induced masculinization of T.rubripes was A2B1C2.The population sex ratio depended on the three factors in the sequence B→A→C.A comparison of the daily increases in length and weight during and after the low-temperature conditions showed that the absolute daily increases in weight and length were significantly less during treatment than after treatment.Daily increases in weight and length did not significantly differ between the treatment groups and the controls(P>0.05),demonstrating that the growth rate could return to normal after the low-temperature conditions.This study establishes a low-temperatureinduced masculinization technology for T.rubripes and demonstrates that although the growth rate(length and weight)decreased in an array of nine treatment groups during the processing time,it returned to a normal level after processing.The results should serve as a guide for achieving the masculinization of T.rubripes in production.展开更多
The original version of this article unfortunately contained a mistake. The publication year in the header on the first page (p.1113) of this article was incorrect. The corrected publication year is given below:
基金supported by The National Natural Science Foundation of China (32001824, 31972198, 31901813, 31901816, 32001827)Startup Fund for Youngman Research at SJTU (SFYR at SJTU)
文摘Pufferfish is prone to deterioration due to abundant nutrients and high moisture content.Drying technology can extend the shelf life and enhance the flavor quality of aquatic products.The study investigated the effect of hot air drying(HAD),microwave vacuum drying(MVD)and hot air assisted radio frequency drying(HARFD)on the taste and volatile profiles of Takifugu obscurus.Different drying methods had significant influence on the color,rehydration,5’-nucleotides,free amino acids and volatile components(P<0.05).The results showed that HAD and HARFD could promote the flavor of T.obscurus by producing higher equivalent umami concentration(EUC)values,which were about two times of MVD group,and more pronounced pleasant odor according to sensory analysis.HAD is more appropriate for industrial application than HARFD and MVD considering the economic benefits.This study could provide a reference for the industrial application of drying T.obscurus.
文摘Objective:To assess the toxicity of the pufferfish Takifugu oblongus,from Chennai coast,Tamil Nadu,India and to detect the presence of Tetrodotoxin(TTX).Methods:The toxicity was evaluated by mouse bioassay using Swiss Albino mice which were expressed in mouse units(MU).Gross anatomical features were observed which is followed by histopathology of the dead mice tissues to establish the toxicity.Instrumental analysis for the presence of tetrodotoxin was also performed through GC-MS and HPLC.Results:The toxicity of ovary was the maximum with 163 MU/g and lowest toxicity was observed in skin with 75.88 MU/g.Histopathological analyses of the dead mice showed various cellular degenerations and inflammations.The amount of Tetrodotoxin detected through GC-MS and HPLC was more reliable and sensitive than the customary mouse bioassay as instrumental analyses were able to detect even nanograms of the toxin.Conclusions:The present study evidently proved that Takifugu oblongus is highly toxic and consumption of the same can pose serious threat for health and possible lethality to humans.
基金the support of the Major Subject of the Committee of Science and Technology of Liaoning Province of China(2011203005)the Public Science and Technology Research Funds Projects of Ocean(2012418025)+4 种基金the Public Subject of the Committee of Science and Technology of Liaoning Province of China(2012005001)the Science and Technology Foundation of Education Department of Liaoning Province of China(L2011117)the Scientific Research Project of Liaoning Provincial Department of Education(L201603)the Open Foundation of Fujian Provincial Key Laboratory of Ecology-Toxicological Effects&Control for Emerging Contaminants(PY16005)the Natural Science Foundation of Liaoning Province of China(2014020149)
文摘Tiger puffer(Takifugu rubripes) is a promising species for aquaculture production because of its high value and limited supply. However, in the north of China, using sea cages to culture this species in winter is hampered by the fact that the seawater temperature is extremely low. Here, a large scale commercial production of tiger puffer has been successfully realized using a recirculating aquaculture system(RAS) from 3 October 2012 to 31 May 2013. The RAS was comprised of nine culture tanks(total water volume 200 m3) and stocked with approximately a total of 14400 fish(initial mean weight 160 g). The tiger puffer was hand-fed at a rate of 0.7% of total body weight per day, and the feed conversion rate was(1.21 ± 0.3) kg kg-1. The recycle water in RAS was treated by a sieve bend screen, a foam fractionator, a submerged biofilter, an UV sterilizer and a submersible aerator. During the whole culture period, an excellent water quality control was achieved in RAS. At the end of this experiment, the survival rate of tiger puffer was more than 98%. The final tank densities averaged 31.2 kg m-3, and the final individual mean weight was 440 g.
基金Supported by the Innovation ofAgricultural Organization and Development of lndustry Amalgamation Project(No.125162002000160001)the Qingdao Postdoctoral Application Research Project(No.ZHHSZ201819039)the Shandong Province Post-doctoral Innovation Projects of Special Funds(No.ZHHSZ201819032)
文摘Estradiol treatment during early life stages of tiger puffer Takifugu rubripes induces feminization in genetic males.However,the ovaries in genetic males may revert to testes once estradiol treatment is halted.Therefore studies should investigate molecular mechanisms underlying ovary-to-testis recovery in genetic males after treatment.In the present study,tiger puffer were exposed to 10,and 100μg/L 17β-estradiol(E 2)from 15 to 100 days post-hatching(dph),then gonad phenotypes and expression profi les of six sex-related genes(cyp19a,foxl2,dmrt1,amh,sox9a,and sox9b)were characterized after the exposure.Results showed that both 10 and 100μg/L E2 induced ovarian development in genetic males at 100 dph.However,all ovaries induced by 10μg/L E2 first developed into intersexual gonads and subsequently reverted to testes after the exposure.As for treatment of 100μg/L E2,while the rest of the ovaries maintained morphological stability,percentages of intersexual gonads reached 38%-57%,and none were reverted to testes.Increased mRNA levels of cyp19a,foxl2 and sox9b and decreased mRNA levels of dmrt1,amh,and sox9a were observed during the ovarian development in genetic males.While contrary gene expression profiles were detected during ovary-to-testis transformation.The mRNA levels of all the six genes were increased during the development of intersexual gonads.These results indicated that up-regulation of dmrt1,amh and sox9a is associated with initial ovary-to-intersexual transformation,and suppression of foxl2,cyp19a and sox9b is essential for complete ovary-to-testis recovery in genetic males.This research will help to trace the molecular processes underlying gonadal transformation in teleosts.
基金Supported by the National Natural Science Foundation of China(No.41606178)the Liaoning Department of Education Key Laboratory of Basic Research Project(No.LZ2015010)the Liaoning Doctor Scientific Research Foundation Project(No.201501185)
文摘This study used tiger puffer Takifugu rubripes to explore new,simple methods for lowtemperature-induced masculinization in a cultured fish without the use of ultraviolet irradiation or sex hormones.An orthogonal test L9(3^4)design was used to consider three factors at three levels:treatment starting times(days post-hatch,dph:factor A)of 20,50 and 80 dph;treatment temperatures(factor B)of 13℃,15℃and 17℃;and treatment durations(factor C)of 30,45 and 60 days.A control group was reared at 21±1℃.The experiments were repeated twice.At 230 dph,the gonads were removed from thirty randomly sampled fish in each group.Histological observations and analysis of single nucleotide polymorphisms(SNP)were used to identified pseudo males,which biological sex was male and genetic sex were female(XX).Treatment group 4(A2B1C2)resulted in the highest proportion of males(75%).According to the intuitive analysis of the orthogonal-array experiments,the optimal combination of lowtemperature-induced masculinization of T.rubripes was A2B1C2.The population sex ratio depended on the three factors in the sequence B→A→C.A comparison of the daily increases in length and weight during and after the low-temperature conditions showed that the absolute daily increases in weight and length were significantly less during treatment than after treatment.Daily increases in weight and length did not significantly differ between the treatment groups and the controls(P>0.05),demonstrating that the growth rate could return to normal after the low-temperature conditions.This study establishes a low-temperatureinduced masculinization technology for T.rubripes and demonstrates that although the growth rate(length and weight)decreased in an array of nine treatment groups during the processing time,it returned to a normal level after processing.The results should serve as a guide for achieving the masculinization of T.rubripes in production.
文摘The original version of this article unfortunately contained a mistake. The publication year in the header on the first page (p.1113) of this article was incorrect. The corrected publication year is given below:
