Objective:To observe the clinical effect of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in the treatment of granulomatous lobular mastitis in the mass stage and the effect on cellu...Objective:To observe the clinical effect of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in the treatment of granulomatous lobular mastitis in the mass stage and the effect on cellular pyroptosis-related proteins.Methods:Forty patients with granulomatous lobular mastitis were randomly divided into 20 cases in the observation group and 20 cases in the control group.Both groups were given dexamethasone ointment for treatment,and the observation group was treated with external application of Zi Cao Yang He Tang on the basis of the control group.Breast lump vol-ume changes,breast pain and pus symptoms,pathology and histology and immunohistochemistry,cellular death-related indicators expression were compared between the two groups.Results:After treatment,the difference in mass volume of the observation group was significantly greater than that of the control group(16.7±21.5 in the control group and 32.9±27.4 in the experimental group)and the difference between the two groups was statistically significant(P<0.05);the objective remission rate of the patients in the observation group(ORR=85%)was significantly higher than that of the treatment group(ORR=50%,P<0.05).In terms of pus formation rate and pain level,the breast pain score of the observation group was significantly lower than that of the control group after two weeks or three weeks of treatment(P<0.05);the pus formation rate of the observation group was 30%,and the pus formation rate of the control group was 20%,and the difference was not statistically significant(P>0.05).The immunohistochemical expression levels of caspase-1 and GSDMD in the observation group were significantly lower than those in the control group(P<0.05);and in terms of the expression of cellular pyroptosis-related indexes,the expression of GSDMD,cas-pase-1,and NLRP3 in the observation group was significantly lower compared with that in the control group(P<0.05).Conclusion:The efficacy of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in treating granulomatous lobular mastitis in the mass stage is remarkable,which can effectively reduce breast pain,reduce the size of the mass,de-crease the expression of cellular pyroptosisrelated indexes,and have a good anti-inflammatory effect.展开更多
目的:观察复方烫疡油(Fu fang tang yang you,FTY)促进小鼠创面愈合、减少瘢痕形成的作用,并探讨其作用机制。方法:采用小鼠深Ⅱ0烫伤模型。创面外用复方烫疡油治疗,并用表皮生长因子(Recombinant human epidermal growth factor,rhEGF...目的:观察复方烫疡油(Fu fang tang yang you,FTY)促进小鼠创面愈合、减少瘢痕形成的作用,并探讨其作用机制。方法:采用小鼠深Ⅱ0烫伤模型。创面外用复方烫疡油治疗,并用表皮生长因子(Recombinant human epidermal growth factor,rhEGF)、磺胺嘧啶银霜、芝麻油(赋形剂)作疗效对比,用生理盐水作空白对照。测定创面组织创伤后8、12、24、48、72h干湿重比例以了解组织创伤后水肿情况;透明膜描绘-求积议测定创面伤后3、7、14h创面愈合情况;并用免疫组织化学法观察Ⅰ、Ⅲ型胶原、TGF-β1(Transforming growth factor-β1,TGF-β1)的变化;TUNEL法定量观察组织细胞的凋亡指数;原位杂交观察胶原酶(Marix Metalloproteianse1,MMP-1)及金属蛋白酶抑制剂(Tissue inhibitor of metalloproteinase-1,TIMP-1)mRNA的表达。结果:复方烫疡油能减轻创面炎症,降低创伤组织水肿,改善Ⅰ、Ⅲ型胶原比例,减少瘢痕形成,促进创面愈合。结论:复方烫疡油具有抗炎、抗感染,促进创面愈合,减轻瘢痕形成的作用。展开更多
基金Hubei Chen Xiaoping Science and Technology Development Foundation Special Fund Project (No.CXPJJH12000002-2020010)Education Science Research Project,Beijing University of Chinese Medicine (No.XJY21056)Beijing University of Chinese Medicine New Teachers Start-up Fund Project (No.2022-BUCMXJKY010)。
文摘Objective:To observe the clinical effect of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in the treatment of granulomatous lobular mastitis in the mass stage and the effect on cellular pyroptosis-related proteins.Methods:Forty patients with granulomatous lobular mastitis were randomly divided into 20 cases in the observation group and 20 cases in the control group.Both groups were given dexamethasone ointment for treatment,and the observation group was treated with external application of Zi Cao Yang He Tang on the basis of the control group.Breast lump vol-ume changes,breast pain and pus symptoms,pathology and histology and immunohistochemistry,cellular death-related indicators expression were compared between the two groups.Results:After treatment,the difference in mass volume of the observation group was significantly greater than that of the control group(16.7±21.5 in the control group and 32.9±27.4 in the experimental group)and the difference between the two groups was statistically significant(P<0.05);the objective remission rate of the patients in the observation group(ORR=85%)was significantly higher than that of the treatment group(ORR=50%,P<0.05).In terms of pus formation rate and pain level,the breast pain score of the observation group was significantly lower than that of the control group after two weeks or three weeks of treatment(P<0.05);the pus formation rate of the observation group was 30%,and the pus formation rate of the control group was 20%,and the difference was not statistically significant(P>0.05).The immunohistochemical expression levels of caspase-1 and GSDMD in the observation group were significantly lower than those in the control group(P<0.05);and in terms of the expression of cellular pyroptosis-related indexes,the expression of GSDMD,cas-pase-1,and NLRP3 in the observation group was significantly lower compared with that in the control group(P<0.05).Conclusion:The efficacy of external application of Zi Cao Yang He Tang combined with dexamethasone ointment in treating granulomatous lobular mastitis in the mass stage is remarkable,which can effectively reduce breast pain,reduce the size of the mass,de-crease the expression of cellular pyroptosisrelated indexes,and have a good anti-inflammatory effect.
文摘目的:观察复方烫疡油(Fu fang tang yang you,FTY)促进小鼠创面愈合、减少瘢痕形成的作用,并探讨其作用机制。方法:采用小鼠深Ⅱ0烫伤模型。创面外用复方烫疡油治疗,并用表皮生长因子(Recombinant human epidermal growth factor,rhEGF)、磺胺嘧啶银霜、芝麻油(赋形剂)作疗效对比,用生理盐水作空白对照。测定创面组织创伤后8、12、24、48、72h干湿重比例以了解组织创伤后水肿情况;透明膜描绘-求积议测定创面伤后3、7、14h创面愈合情况;并用免疫组织化学法观察Ⅰ、Ⅲ型胶原、TGF-β1(Transforming growth factor-β1,TGF-β1)的变化;TUNEL法定量观察组织细胞的凋亡指数;原位杂交观察胶原酶(Marix Metalloproteianse1,MMP-1)及金属蛋白酶抑制剂(Tissue inhibitor of metalloproteinase-1,TIMP-1)mRNA的表达。结果:复方烫疡油能减轻创面炎症,降低创伤组织水肿,改善Ⅰ、Ⅲ型胶原比例,减少瘢痕形成,促进创面愈合。结论:复方烫疡油具有抗炎、抗感染,促进创面愈合,减轻瘢痕形成的作用。
