Experimental study on Tangshenjiangzhuo granules treatment of rats with early diabetic nephropathy

Objective:To study the effect of Tangshenjiangzhuo granules on serum cytokines as well as the oxidative stress and endoplasmic reticulum stress in kidney tissue of diabetic rats. Methods:SPF male Wistar rats were selected as experimental animals and randomly divided into control group, model group and intervention group. Model group received intragastric administration of saline after diabetes model was established, and the intervention group received intragastric administration of Tangshenjiangzhuo granules after diabetes model was established. 8 weeks after treatment, serum was collected to detect the levels of pro-inflammatory cytokines and adipocytokines, and kidney tissue was collected to detect the levels of oxidative stress molecules and endoplasmic reticulum stress molecules. Results:Serum interleukin-6 (IL-6), macrophage migration inhibitory factor (MIF), regulated upon activation normal T cell expressed secreted (RANTES), Chemerin and Nesfatin levels of model group were significantly higher than those of control group (P<0.05), and serum IL-6, MIF, RANTES, Chemerin and Nesfatin levels of intervention group were significantly lower than those of model group (P<0.05);reactive oxygen species (ROS), malondialdehyde (MDA), advanced oxidation protein products (AOPP), 8-hydroxy-2-deoxyguanosine (8-OHdG), glucose-regulated protein (GRP78), CCAAT/enhancer-binding protein homologous protein (CHOP) and ATF4 protein levels in kidney tissue of model group were significantly higher than those of control group (P<0.05), and ROS, MDA, AOPP, 8-OHdG, GRP78, CHOP and ATF4 levels in kidney tissue of intervention group were significantly lower than model group (P<0.05). Conclusions:Tangshenjiangzhuo granules can adjust the secretion of pro-inflammatory factors and adipocytokines, and inhibit oxidative stress and endoplasmic reticulum stress in kidney tissue of diabetic rat models.

糖肾降浊颗粒治疗早期糖尿病肾病大鼠的实验研究

目的:研究糖肾降浊颗粒对糖尿病大鼠血清细胞因子以及肾脏组织氧化应激反应、内质网应激反应的影响。方法:选择SPF级的雄性Wistar大鼠作为实验动物,随机分为对照组、模型组及干预组,模型组建立糖尿病模型后给予生理盐水灌胃,干预组建立糖尿病模型后给予糖肾降浊颗粒灌胃治疗。治疗8周后,采集血清并检测促炎因子、脂肪因子的含量,采集肾脏组织并检测氧化应激分子、内质网应激分子的含量。结果:模型组大鼠血清中IL-6、MIF、RANTES、Chemerin、Nesfatin的含量均显著高于对照组,干预组大鼠血清中IL-6、MIF、RANTES、Chemerin、Nesfatin的含量均显著低于模型组;模型组大鼠肾脏组织中ROS、MDA、AOPP、8-OHdG、GRP78、CHOP、ATF4的含量均显著高于对照组,干预组大鼠肾脏组织中ROS、MDA、AOPP、8-OHdG、GRP78、CHOP、ATF4的含量均显著低于模型组。结论:糖肾降浊颗粒干预能够调节糖尿病大鼠模型体内促炎因子和脂肪因子的分泌,抑制肾脏组织的氧化应激反应和内质网应激反应。