On the Study of Multi-Gas Data Acquisition System Based on PCI1711 Card

A data acquisition system for testing gas sensor array response to multi-gas is presented.The testing system is based on the character of the gas response of metal oxide semiconductor gas sensor array.The data acquisition is realized automatically through the real time controlling of the data acquisition card PCI1711.This system is highly attractive for electronic nose,which is a powerful tool for the discrimination of gases.

赣州市呼吸道伴发热症状病例的多病原研究

目的探讨赣州市呼吸道伴发热症状病例的病原阳性检出情况及病原特点。方法选取2021年1月至2022年3月赣州市疾病预防控制中心收集的198例不明原因呼吸道伴发热症状患者的咽拭子样本进行研究。采用基于微流体芯片(TAC)的核酸快速检测技术进行病原体检测,记录阳性检出率以及病原类型,分析病原分布特点。结果在198例患者中,0~<6岁人群的阳性检出率高于6~<18、18~<46、46~65岁人群,年龄>65岁人群的阳性检出率高于6~<18岁人群,差异有统计学意义(P<0.005)。本次共检出14种病毒以及7种细菌,主要病原类型为流感嗜血杆菌、人类疱疹病毒4型(HHV-4)、肺炎克雷伯菌、腮腺炎病毒、乙型流感病毒。在各年龄段人群中,0~<6岁人群的HHV-4、流感嗜血杆菌阳性检出率较高,>65岁人群的肺炎克雷伯菌、乙型流感病毒阳性检出率较高,6~<47岁人群的腮腺炎病毒阳性检出率较高。不同年龄段的主要病原类型分布阳性检出率比较,差异有统计学意义(P<0.05)。在198例患者中,体温正常95例,低热61例,中度发热20例,高热22例。中度发热、高热人群的主要病原阳性检出率高于正常、低热人群,差异有统计学意义(P<0.008)。结论赣州市呼吸道伴发热症状者的病毒/细菌阳性检出率以婴幼儿和老年人居多,不同年龄段人群的病原分布存在差异,且体温与阳性检出率有关。

2种高通量核酸检测方法多种呼吸道病原体检测效能比较

目的比较多重荧光定量聚合酶链反应(PCR)和TaqMan微流体芯片(TAC)技术检测呼吸道病原体的效能。方法收集419例急性呼吸道感染患者临床样本,分别采用TAC技术和多重荧光定量PCR进行多种呼吸道病毒核酸检测,比较2种方法的敏感性和特异性。结果多重荧光定量PCR多种呼吸道病毒的阳性检出率为30.1%,敏感性为93.4%,特异性为98.9%;TAC技术多种呼吸道病毒阳性检出率为27.9%,敏感性为87.4%,特异性为98.9%。2种方法检测结果的一致性为92.8%,且敏感性和特异性差异均无统计学意义(P>0.05)。结论 TAC技术与多重荧光定量PCR检测常见呼吸道病毒的敏感性和特异性相近,但TAC技术检测病原谱范围更广,在紧急情况下可作为快速筛查多种呼吸道病原体的有效方法。

Trend of <i>Cryptosporidium</i>Infection among Children below 24 Months in an Informal Urban Settlement, Kenya

Cryptosporidium infection is estimated to cause 2.9 million diarrheal cases yearly among children aged under 24 months in sub-Saharan Africa. Studies have shown long-term climatic variations can affect infectious diseases. The burden of cryptosporidiosis in rural areas of sub-Saharan Africa is well characterized. However, the trend of Cryptosporidium infection is not known, especially in informal urban settings. This study therefore sought to determine cryptosporidiosis trends, and further explore the association between year and Cryptosporidium infection among children below 24 months in Kibera urban informal settlement in Kenya. Data collected by the Kenya Medical Research Institute longitudinal study in Tabitha clinic in Kibera from 2009 to 2015 were used. At least 3000 children aged < 24 months receive free health care at the clinic. In the longitudinal study, children presenting with diarrhea were eligible for stool sample collection (n = 477), out of which 421 stool samples were tested using TaqMan™ Array Card (TAC) polymerase chain reaction panel that included a target for Cryptosporidium genus. Data for the 421 children were included in the analysis. Logistic regression was used to explore the difference between the seven years and cryptosporidiosis. Overall, the pooled data indicated that 23.5% of the children who were tested had Cryptosporidium infection, with the highest proportions of Cryptosporidium-positive cases observed in 2015 (45.2%). The logistic regression results also indicated that children who were tested in the year 2015 were more likely to have Cryptosporidium infection (OR = 3.39;95% CI: 1.44 - 7.96;p = 0.005) than those in 2009. Watery stool was also found to be an important symptom of cryptosporidiosis. There was a high prevalence of Cryptosporidium infection among young children, especially in the most recent year. Routine testing of Cryptosporidium infection using molecular methods, constant monitoring and identification of the infection sources is therefore necessary towards reducing the disease burden in the low resource settings.

精神分裂症血浆中差异表达miRNA的辅助诊断价值初探

目的:检测与精神分裂症相关的血浆中差异表达微小RNA(miRNA),探寻有精神分裂症诊断价值的生物标记。方法:选择6例首发、未用药的符合美国精神障碍诊断与统计手册第4版(DSMIV)诊断的精神分裂症患者及6例年龄、性别匹配的正常对照者(筛查样本),采用Taq M an Array人类微小RNA芯片(Taq Man Array Human MicroRNA A+B Cards Set v3.0)高通量检测了754个miRNA(SangerhumanmiRBase v14)的血浆表达水平,并用SAM软件的t检验分析病例和对照组间miRNA表达水平的差异;进而在独立的25例精神分裂症患者和18例正常对照(验证样本)中,采用实时荧光定量PCR(RT-PCR)方法进行检测与分析,采用t检验比较两组miRNA表达水平差异。结果:筛查样本中精神分裂症患者有20个miRNA的表达水平低于正常对照,并通过FDR多重比较校正;根据差异表达miRNA的显著性和既往文献报道,选择其中8个差异表达miRNA进行独立样本验证,发现这些miRNA在验证样本的精神分裂症血浆中表达下调,且其中4个miRNA:hsa-miR-15b-5p经过FDR多重比较校正后仍有统计意义。结论:精神分裂症患者血浆中有多个miRNA低表达,这些低表达的miRNA对精神分裂症的诊断有参考意义。

Identification of deregulated miRNAs and their targets in hepatitis B virus-associated hepatocellular carcinoma

AIM： TO identify the differentially expressed miRNAs and their targets in hepatitis B virus （HBV）-associated hepatocellular carcinoma （HCC）. METHODS： Six hundred and sixty seven human miRNAs were quantitatively analyzed by Taqman lowdensity miRNA array （TLDA） in HBV-HCC tissues. Gene ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analyses were used to analyze the significant function and pathway of the differentially expressed miRNAs in HBV-HCC. TargetScan software was used to predict the targets of deregulated miRNAs. Western blotting and luciferase assay were performed to verify the targets of these miRNAs.RESULTS： Ten up-regulated miRNAs （miR-217, miR- 518b, miR-517c, miR-520g, miR-519a, miR-522, miR- 518e, miR-525-3p, miR-512-3p, and miR-518a-3p） and 11 down-regulated miRNAs （miR-138, miR-214, miR-214#, miR-199a-5p, miR-433, miR-511, miR-592, miR-483-3p, miR-483-5p, miR-708 and miR-1275） were identified by Taqman miRNAs array and confirmed quantitatively by reverse transcription polymerase chain reaction in HCC and adjacent non-tumor tissues. GO and KEGG pathway analysis revealed that ＂regulation of actin cytoskeleton＂ and ＂pathway in cancer＂ are most likely to play critical roles in HCC tumorigenesis. MiR- 519a and ribosomal protein S6 kinase polypeptide 3 （RPS6KA3） were predicted as the most significant can-didates by miRNA-mRNA network. In addition, cyclin D3 （CCND3） and clathrin heavy chain （CHC）, usually up-regulated in HCC tissues, were validated as the di- rect target of miR-138 and miR-199a-5p, respectively.

TaqMan微流体芯片技术应用发展

分子诊断技术日新月异,TaqMan微流体芯片作为一种新兴的病原体筛查技术,具有快速高效、灵敏准确、检测范围广等特点,应用研究也逐年增加,在疾病监测、临床诊疗、食品卫生领域表现尤为突出。本文将针对TaqMan微流体芯片技术应用发展进行综述,为广大科研工作者提供研究新方向、新思路。

Taqman低密度微流体芯片技术在呼吸道传染病病原体快速检测中的应用分析

目的使用Taqman低密度微流体芯片技术(TAC)建立顺义区多种呼吸道传染病病原体构成谱,为制定有效防控措施提供依据。方法利用Taqman低密度微流体芯片技术,对2017年收集的372份呼吸道感染病例标本进行42种呼吸道传染病病原体的检测。结果 372份呼吸道感染患者病例标本共检出16种病原体,检出阳性病原体125例,阳性率为33.6%(125/372)。在所检出的病原体构成谱中,肺炎支原体检出阳性率最高;其次为流感病毒及肺炎链球菌,肺炎克雷伯菌、金黄色葡萄球菌等其他病原体也有检出,并随着年龄和季节变化感染病原体有一定的流行规律。结论肺炎支原体、流感病毒、肺炎链球菌是顺义区呼吸道感染病的主要病原体,今后应继续加强病原学的监测,注重病原体在不同人群、季节感染分布以及防治工作。

10 Gb/s free space optical interconnect with broadcasting capability enabled by a silicon integrated optical phased array

We have proposed and experimentally demonstrated a reconfigurable free space optical interconnect with broadcasting capability based on an eight-channel silicon integrated optical phased array.By using the silicon integrated beam steering and broadcasting device,10 Gb/s on-off keying data is transmitted over 15 cm in free space for up to three receivers located in three different cards.The experimental results show that the optical phased array can be used with broadcasting capability provided to multi-receivers in the card to card optical interconnects,which can significantly reduce device size,system complexity,and total costs.

流行性脑膜炎患者密切接触人群呼吸道样本多病原检测结果分析

目的 探究脑膜炎奈瑟菌携带情况与各种呼吸道病原体携带情况之间的关联性,为后续研究提供理论基础。方法 采集2名流脑患者的60例密切接触者咽拭子样本,采用荧光PCR法检测脑膜炎奈瑟菌携带情况,同时利用TaqMan微流体芯片检测多种呼吸道病原体。结果 采用荧光PCR法检出脑膜炎奈瑟菌阳性32例,阳性率53.33%。利用微流体芯片检出的病原体携带率前5位分别为流感嗜血杆菌(58/60,96.67%)、2型鼻病毒(47/60,78.33%)、1型鼻病毒(45/60,75.00%)、肺炎克雷伯菌(35/60,58.33%)、肺炎链球菌(29/60,48.33%)。在阳性组与阴性组检出率对比中,2型鼻病毒(χ^(2)=6.104,P=0.013)与卡它莫拉氏菌(χ^(2)=4.627,P=0.031)的检出率差异均有统计学意义。结论 脑膜炎奈瑟菌携带与2型鼻病毒、卡它莫拉氏菌携带存在统计学关联。

多重PCR技术和呼吸道病原体微流体芯片在诊断儿童急性呼吸道病毒感染中的应用

目的 了解多重PCR技术(RespiFinder■SMART 22检测试剂盒)和呼吸道病原体微流体芯片技术(TaqMan array cards, TAC)在诊断儿童急性呼吸道感染(acute respiratory infection, ARI)中的应用价值。方法 从2018年8月份至2019年8月份采集的成都市儿童专科医院275例有ARI症状的患儿咽拭子样本中随机选择120份样本,分别使用RespiFinder■SMART 22和TAC进行呼吸道病毒核酸检测,比较2种检测方法的优缺点。结果 120份儿童ARI标本经RespiFinder■SMART 22和TAC检测,阳性率分别为88.33%和93.33%,混合感染率分别为26.67%和35%。在120份样本中,2种方法检测结果一致率为88.33%,其中阳性一致性106例,阴性一致性7例。结果不一致的有7例。结论 多重PCR技术和TAC技术在此次实验检测中检出阳性样本一致性较高,两者对于呼吸道病毒检测均具有良好的临床应用价值。