Two new flavone glycosides, isoetin-7-O-β-D-glucopyranosyl-2′-O-α-L-arabinopyranoside （1） and isoetin-7-O-β-D-glucopyranosyl-2′-O-α-D-glucopyranoside （2）, a new lignan, mongolicumin A （3）, and a new guaian...Two new flavone glycosides, isoetin-7-O-β-D-glucopyranosyl-2′-O-α-L-arabinopyranoside （1） and isoetin-7-O-β-D-glucopyranosyl-2′-O-α-D-glucopyranoside （2）, a new lignan, mongolicumin A （3）, and a new guaianolide, mongolicumin B （4） were isolated from the aerial part of Taraxacum mongolicum. Their structures were elucidated mainly by spectral analyses.展开更多
Plants belonging to the genus Taraxacum are widespread all over the world,which contain rubber-producing and non-rubberproducing species.However,the genomic basis underlying natural rubber(NR)biosynthesis still needs ...Plants belonging to the genus Taraxacum are widespread all over the world,which contain rubber-producing and non-rubberproducing species.However,the genomic basis underlying natural rubber(NR)biosynthesis still needs more investigation.Here,we presented high-quality genome assemblies of rubber-producing T.kok-saghyz TK1151 and non-rubber-producing T.mongolicum TM5.Comparative analyses uncovered a large number of genetic variations,including inversions,translocations,presence/absence variations,as well as considerable protein divergences between the two species.Two gene duplication events were found in these two Taraxacum species,including one common ancestral whole-genome triplication and one subsequent round of gene amplification.In genomes of both TK1151 and TM5,we identified the genes encoding for each step in the NR biosynthesis pathway and found that the SRPP and CPT gene families have experienced a more obvious expansion in TK1151 compared to TM5.This study will have large-ranging implications for the mechanism of NR biosynthesis and genetic improvement of NR-producing crops.展开更多
DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbit...DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.展开更多
文摘Two new flavone glycosides, isoetin-7-O-β-D-glucopyranosyl-2′-O-α-L-arabinopyranoside （1） and isoetin-7-O-β-D-glucopyranosyl-2′-O-α-D-glucopyranoside （2）, a new lignan, mongolicumin A （3）, and a new guaianolide, mongolicumin B （4） were isolated from the aerial part of Taraxacum mongolicum. Their structures were elucidated mainly by spectral analyses.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA24030504 and XDA24040305)the National Natural Science Foundation of China(32000144)the Key Research Program of the Chinese Academy of Sciences(ZDRW-ZS-2018-2)。
文摘Plants belonging to the genus Taraxacum are widespread all over the world,which contain rubber-producing and non-rubberproducing species.However,the genomic basis underlying natural rubber(NR)biosynthesis still needs more investigation.Here,we presented high-quality genome assemblies of rubber-producing T.kok-saghyz TK1151 and non-rubber-producing T.mongolicum TM5.Comparative analyses uncovered a large number of genetic variations,including inversions,translocations,presence/absence variations,as well as considerable protein divergences between the two species.Two gene duplication events were found in these two Taraxacum species,including one common ancestral whole-genome triplication and one subsequent round of gene amplification.In genomes of both TK1151 and TM5,we identified the genes encoding for each step in the NR biosynthesis pathway and found that the SRPP and CPT gene families have experienced a more obvious expansion in TK1151 compared to TM5.This study will have large-ranging implications for the mechanism of NR biosynthesis and genetic improvement of NR-producing crops.
文摘DNA fingerprinting among members of the Chinese drug Pu Gong Ying(Taraxacum mongolicum Hand,-Mazz.)and six adulterants of Tu Gong Ying were demonstrated with random-primed polymerase chain reaction(PCR)including arbitrarily primed polymerase chain reaction(AP-PCR)and random amplified polymorphic DNA(RAPD).Distinctive,reproducible genomic fingerprints from DNA from 7 species belonged to Compositae were generated with two long(20 and 24 mer)and one short(10 mer)randomly chosen primers.The Pu Gong Ying can be differentiated from six species of Tu Gong Ying according to the banding pattems of their amplified DNA on agarose gels.The results showed that AP-PCR and RAPD methods can be used for identifying Chinese drugs.Moreover,the Similarity Indexes of the genomic DNA fingerprints showed that Pu Gong Ying and its adulterants are unrelated.Therefore,AP-PCR and RAPD methods can be used for identifying Chinese drugs.
