In the flotation process, bubble is a key factor in studying bubble-particle interaction and fine particle flo- tation. Knowledge on size distribution of bubbles in a flotation system is highly important. In this stud...In the flotation process, bubble is a key factor in studying bubble-particle interaction and fine particle flo- tation. Knowledge on size distribution of bubbles in a flotation system is highly important. In this study, bubble distributions in different reagent concentrations, electrolyte concentrations, cathode apertures, and current densities in electroflotation are determined using a high-speed camera. Average bubble sizes under different conditions are calculated using Image-Pro@ Plus （Media Cybernetics@, MD, USA） and SigmaScan@ Pro （Systat Software, CA, USA） software. Results indicate that the average sizes of bubbles, which were generated through 38, 50, 74, 150, 250, 420, and 1000 μm cathode apertures, are 20.2, 29.5, 44.6, 59.2, 68.7, 78.5, and 88.8 μm, respectively. The optimal current density in electroflotation is 20 A/m2. Reagent and electrolyte concentrations, current density, and cathode aperture are important factors in controlling bubble size and nucleation. These factors also contribute to the control of fine- Particle flotation.展开更多
目的:研究Tat作用蛋白30(Tat-interactive protein 30,TIP30)基因在胃癌组织中的表达及其对胃癌血管生成的影响。方法:运用免疫组织化学方法检测52例胃癌组织及47例癌旁正常组织中TIP30蛋白的表达水平,同时检测CD34标记的微血管密度(mic...目的:研究Tat作用蛋白30(Tat-interactive protein 30,TIP30)基因在胃癌组织中的表达及其对胃癌血管生成的影响。方法:运用免疫组织化学方法检测52例胃癌组织及47例癌旁正常组织中TIP30蛋白的表达水平,同时检测CD34标记的微血管密度(microvessel density,MVD)。结果:TIP30在胃癌和癌旁正常组织中的阳性率分别为53.8%和85.1%,两者相比较差异有统计学意义(χ2=11.22,P﹤0.01),TIP30表达水平与患者年龄、性别、病理分化程度及肿瘤大小无关,与有无淋巴结转移及TNM分期有关。胃癌组织和癌旁正常组织中MVD值分别为27.37±2.68和21.87±4.11,两者比较差异有统计学意义(t=7.95,P<0.01),胃癌组织中TIP30表达阳性者较阴性者MVD值显著降低(t=-3.18,P=0.003)。结论:TIP30在胃癌组织中表达降低,其可能通过抑制胃癌血管生成影响胃癌的发生发展。展开更多
Calcium phosphate nanoparticles(CaPNPs)have good biocompatibility as gene carriers;however,CaPNPs typically exhibit a low transfection efficiency.Cell penetrate peptide(TAT)can increase the uptake of nanoparticles but...Calcium phosphate nanoparticles(CaPNPs)have good biocompatibility as gene carriers;however,CaPNPs typically exhibit a low transfection efficiency.Cell penetrate peptide(TAT)can increase the uptake of nanoparticles but is limited by its non-specificity.Grafting adhesion peptide adhesion peptide on carriers can enhance their targeting.The Plekho1 gene encodes casein kinase-2 interacting protein-1(CKIP-1),which can negatively regulate osteogenic differentiation.Based on the above,we produced a Mg-CaPNPs-RGD-TAT-CKIP-1 siRNA carrier system via hydrothermal synthesis,silanization and adsorption.The effects of this carrier system on cell endocytosis and biological effects were evaluated by cell culture in vitro.The results demonstrate that CaPNPs with 7%Mg(60 nm particle size,short rod shape and good dispersion)were suitable for use as gene carriers.The carrier system boosted the endocytosis of MG63 cells and was helpful for promoting the differentiation of osteoblasts,and the dual-ligand system possessed a synergistic effect.The findings of this study show the tremendous potential of the Mg-CaPNPs-RGD-TAT-CKIP-1 siRNA carrier system for efficient delivery into cells and osteogenesis inducement.展开更多
目的探讨分析Notch3与TIP30表达对脑胶质瘤患者预后评估价值。方法收集2019年12月至2020年12月期间保定市第七医院手术切除经病理科确诊的135例脑胶质瘤患者病理组织标本135份,并取瘤旁组织60份。采用蛋白质印迹法(western blot)和免疫...目的探讨分析Notch3与TIP30表达对脑胶质瘤患者预后评估价值。方法收集2019年12月至2020年12月期间保定市第七医院手术切除经病理科确诊的135例脑胶质瘤患者病理组织标本135份,并取瘤旁组织60份。采用蛋白质印迹法(western blot)和免疫组化法检测脑胶质瘤组织中和瘤旁组织中Notch3和Tat(transactivator of transcription)作用蛋白30(Tat-interactive protein 30,TIP30)表达情况。比较不同组织中Notch3、TIP30阳性率情况及Notch3、TIP30表达与脑胶质瘤临床病理特征的关系。随访术后1年患者生存情况,采用Kaplan-Meier法分析Notch3、TIP30表达与脑胶质瘤患者预后的关系,并采用Spearman等级相关性分析Notch3与TIP30表达的关系。结果胶质瘤组织中Notch3蛋白表达量(2.25±0.63)高于瘤旁组织的(0.95±0.21),Notch3阳性率57.78%高于瘤旁组织的36.67%,而TIP30蛋白表达量(0.42±0.11)低于瘤旁组织的(0.71±0.23),TIP30阳性率48.15%显著低于瘤旁组织的86.67%(P<0.05)。胶质瘤组织中Notch3阳性表达与患者患者性别、肿瘤部位、切除范围无关(P>0.05),与患者年龄、肿瘤直径、病理分级、Ki-67表达有关(P<0.05);胶质瘤组织中TIP30阳性表达与患者性别、年龄、肿瘤部位、切除范围无关(P>0.05),与肿瘤直径、病理分级、Ki-67表达有关(P<0.05)。Cox分析结果显示,病理分级(HR=2.401,95%CI:1.572~3.667)、Ki-67高表达(2.492,95%CI:1.542~4.028)、Notch3高表达(HR=2.221,95%CI:1.138~4.333)、TIP30低表达(HR=8.457,95%CI:2.050~34.886)是影响脑胶质瘤患者预后的重要影响因素(P<0.05)。Kaplan-Meier生存分析,结果显示,Notch3高表达(评分<2分)组脑胶质瘤患者1年生存时间低于低表达(评分>2分)组(P<0.05);TIP30高表达(评分>3分)组脑胶质瘤患者1年生存时间高于低表达(评分<3分)组(P<0.05)。Spearman等级相关性分析,结果发现,脑胶质瘤组织中Notch3和TIP30表达呈负相关(r=-0.539,P<0.01)。结论脑胶质瘤患者组织中Notch3呈较高表达,TIP30呈较低表达,随着病理分级增加Notch3表达越高,TIP30表达降低,患者预后较差,其表达对早期病情及预后评估具有重要参考价值。展开更多
基金the National Natural Science Foundation of China (No.50774094)the Ministry of Science and Technology of China (No.2011BAB05B01) for financial support
文摘In the flotation process, bubble is a key factor in studying bubble-particle interaction and fine particle flo- tation. Knowledge on size distribution of bubbles in a flotation system is highly important. In this study, bubble distributions in different reagent concentrations, electrolyte concentrations, cathode apertures, and current densities in electroflotation are determined using a high-speed camera. Average bubble sizes under different conditions are calculated using Image-Pro@ Plus （Media Cybernetics@, MD, USA） and SigmaScan@ Pro （Systat Software, CA, USA） software. Results indicate that the average sizes of bubbles, which were generated through 38, 50, 74, 150, 250, 420, and 1000 μm cathode apertures, are 20.2, 29.5, 44.6, 59.2, 68.7, 78.5, and 88.8 μm, respectively. The optimal current density in electroflotation is 20 A/m2. Reagent and electrolyte concentrations, current density, and cathode aperture are important factors in controlling bubble size and nucleation. These factors also contribute to the control of fine- Particle flotation.
文摘目的:研究Tat作用蛋白30(Tat-interactive protein 30,TIP30)基因在胃癌组织中的表达及其对胃癌血管生成的影响。方法:运用免疫组织化学方法检测52例胃癌组织及47例癌旁正常组织中TIP30蛋白的表达水平,同时检测CD34标记的微血管密度(microvessel density,MVD)。结果:TIP30在胃癌和癌旁正常组织中的阳性率分别为53.8%和85.1%,两者相比较差异有统计学意义(χ2=11.22,P﹤0.01),TIP30表达水平与患者年龄、性别、病理分化程度及肿瘤大小无关,与有无淋巴结转移及TNM分期有关。胃癌组织和癌旁正常组织中MVD值分别为27.37±2.68和21.87±4.11,两者比较差异有统计学意义(t=7.95,P<0.01),胃癌组织中TIP30表达阳性者较阴性者MVD值显著降低(t=-3.18,P=0.003)。结论:TIP30在胃癌组织中表达降低,其可能通过抑制胃癌血管生成影响胃癌的发生发展。
基金Project(81571021)supported by the National Natural Science Foundation of ChinaProject(2018zzts944)supported by the Graduate Student Independent Exploration Innovation Fund of the Central South University,China+1 种基金Projects(2015WK3012,2018SK2017)supported by the Hunan Provincial Science and Technology Department,ChinaProject(20160301)supported by New Talent Project of the Third Xiangya Hospital of Central South University,China。
文摘Calcium phosphate nanoparticles(CaPNPs)have good biocompatibility as gene carriers;however,CaPNPs typically exhibit a low transfection efficiency.Cell penetrate peptide(TAT)can increase the uptake of nanoparticles but is limited by its non-specificity.Grafting adhesion peptide adhesion peptide on carriers can enhance their targeting.The Plekho1 gene encodes casein kinase-2 interacting protein-1(CKIP-1),which can negatively regulate osteogenic differentiation.Based on the above,we produced a Mg-CaPNPs-RGD-TAT-CKIP-1 siRNA carrier system via hydrothermal synthesis,silanization and adsorption.The effects of this carrier system on cell endocytosis and biological effects were evaluated by cell culture in vitro.The results demonstrate that CaPNPs with 7%Mg(60 nm particle size,short rod shape and good dispersion)were suitable for use as gene carriers.The carrier system boosted the endocytosis of MG63 cells and was helpful for promoting the differentiation of osteoblasts,and the dual-ligand system possessed a synergistic effect.The findings of this study show the tremendous potential of the Mg-CaPNPs-RGD-TAT-CKIP-1 siRNA carrier system for efficient delivery into cells and osteogenesis inducement.
文摘目的探讨分析Notch3与TIP30表达对脑胶质瘤患者预后评估价值。方法收集2019年12月至2020年12月期间保定市第七医院手术切除经病理科确诊的135例脑胶质瘤患者病理组织标本135份,并取瘤旁组织60份。采用蛋白质印迹法(western blot)和免疫组化法检测脑胶质瘤组织中和瘤旁组织中Notch3和Tat(transactivator of transcription)作用蛋白30(Tat-interactive protein 30,TIP30)表达情况。比较不同组织中Notch3、TIP30阳性率情况及Notch3、TIP30表达与脑胶质瘤临床病理特征的关系。随访术后1年患者生存情况,采用Kaplan-Meier法分析Notch3、TIP30表达与脑胶质瘤患者预后的关系,并采用Spearman等级相关性分析Notch3与TIP30表达的关系。结果胶质瘤组织中Notch3蛋白表达量(2.25±0.63)高于瘤旁组织的(0.95±0.21),Notch3阳性率57.78%高于瘤旁组织的36.67%,而TIP30蛋白表达量(0.42±0.11)低于瘤旁组织的(0.71±0.23),TIP30阳性率48.15%显著低于瘤旁组织的86.67%(P<0.05)。胶质瘤组织中Notch3阳性表达与患者患者性别、肿瘤部位、切除范围无关(P>0.05),与患者年龄、肿瘤直径、病理分级、Ki-67表达有关(P<0.05);胶质瘤组织中TIP30阳性表达与患者性别、年龄、肿瘤部位、切除范围无关(P>0.05),与肿瘤直径、病理分级、Ki-67表达有关(P<0.05)。Cox分析结果显示,病理分级(HR=2.401,95%CI:1.572~3.667)、Ki-67高表达(2.492,95%CI:1.542~4.028)、Notch3高表达(HR=2.221,95%CI:1.138~4.333)、TIP30低表达(HR=8.457,95%CI:2.050~34.886)是影响脑胶质瘤患者预后的重要影响因素(P<0.05)。Kaplan-Meier生存分析,结果显示,Notch3高表达(评分<2分)组脑胶质瘤患者1年生存时间低于低表达(评分>2分)组(P<0.05);TIP30高表达(评分>3分)组脑胶质瘤患者1年生存时间高于低表达(评分<3分)组(P<0.05)。Spearman等级相关性分析,结果发现,脑胶质瘤组织中Notch3和TIP30表达呈负相关(r=-0.539,P<0.01)。结论脑胶质瘤患者组织中Notch3呈较高表达,TIP30呈较低表达,随着病理分级增加Notch3表达越高,TIP30表达降低,患者预后较差,其表达对早期病情及预后评估具有重要参考价值。
