This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the ...This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the effects of NAA,6-BA,IBA and KT on the induction of differentiation,proliferation and rooting were evaluated on MS or 1/2 MS medium. The results showed that Zhongshanshan can be proliferated via tissue culture. The combined use of NAA and 6-BA in MS medium greatly promoted the differentiation of Zhongshanshan explants,and the optimal medium was MS ＋ 0. 2 mg/L NAA ＋ 0. 4 mg/L6-BA. The optimal medium for the proliferation of differentiated buds was MS ＋ 0. 3 mg/L NAA ＋ 0. 4 mg/L 6-BA,on which the proliferation rate was up to 3. 8. 1/2 MS medium was more conducive than MS medium to the induction of rooting. The optimal medium for the rooting of tissue-cultured Zhongshanshan shoots was 1/2 MS ＋ 0. 3 mg/L IBA ＋ 0. 2 mg/L NAA.展开更多
Saltwater intrusion is a major cause of coastal erosion. New hybrid bald cypress species have been shown to exhibit salinity tolerance compared to native bald cypress species. Planting these hybrids, or Nanjing Bot Ga...Saltwater intrusion is a major cause of coastal erosion. New hybrid bald cypress species have been shown to exhibit salinity tolerance compared to native bald cypress species. Planting these hybrids, or Nanjing Bot Garden genotype (NBG), trees where their roots can stabilize soil and preserve land may reduce coastal erosion in areas subjected to saltwater intrusion. The purpose of this experiment was to determine whether or not selected bald cypress NBG genotype tree species survive better than native bald cypress trees in areas inundated with saline water. Leaves were harvested, dried and analyzed for their elemental concentration. NBG genotype trees excluded more sodium than native bald cypress trees. NBG genotype tree roots were also less effected by brackish water. Finally, the growth of the NBG genotype trees and the control were measured. All NBG genotype trees tested showed growth while the control trees maintained little or no growth, and a decrease in tree height in sodic soil. If these NBG genotype trees are subjected to a surge of brackish water, NBG genotype bald cypress trees may be used to stabilize soil that may be otherwise subject to coastal erosion.展开更多
采用室内盆栽方式,以两年生落羽杉属杂交树种"中山杉406"(Taxodium‘Zhongshanshan 406’)为试验材料,设置土壤含水量为土壤田间持水量的70%(对照)、渍水(水面与土面持平)、淹水10 cm 3个处理,研究中山杉406的生物量,N、P等...采用室内盆栽方式,以两年生落羽杉属杂交树种"中山杉406"(Taxodium‘Zhongshanshan 406’)为试验材料,设置土壤含水量为土壤田间持水量的70%(对照)、渍水(水面与土面持平)、淹水10 cm 3个处理,研究中山杉406的生物量,N、P等营养元素含量,以及超氧化物歧化酶(SOD)活性等生长与生理指标的变化。结果表明,与对照相比,渍水与淹水能显著增加中山杉406的地茎,降低植株的根冠比,而对植株的株高、鲜质量、株高增长率与鲜质量增长率无显著影响。淹水处理后,中山杉406根部P、K、Na、Mg含量显著高于对照。中山杉406叶片的叶绿素含量、SOD活性、过氧化物酶(POD)活性、丙二醛(MDA)含量以及根系活力在对照、渍水、淹水3个处理间无显著差异。此外,淹水处理中山杉406出现皮孔肥大以及长出气生根等对淹水的适应特征。可见,中山杉406具有良好的耐水湿特性,可应用于湿地的恢复与构建。展开更多
为了探究水杉(Metasequoia glyptostroboides Hu et Cheng)、池杉(Taxodium ascendens Brongn.)和落羽杉(T.distichum(Linn.)Rich.)挥发性有机化合物(VOCs)的差异,从植物挥发性有机化合物角度为其园林配置提供理论依据,试验采用动态顶...为了探究水杉(Metasequoia glyptostroboides Hu et Cheng)、池杉(Taxodium ascendens Brongn.)和落羽杉(T.distichum(Linn.)Rich.)挥发性有机化合物(VOCs)的差异,从植物挥发性有机化合物角度为其园林配置提供理论依据,试验采用动态顶空气体循环采集法和热脱附气质联用技术(TDS-GC-MS)对3种杉科植物挥发物进行成分分析。结果表明:萜烯类化合物是3种杉科植物挥发性有机化合物的主要成分。其中从水杉中鉴定出26种成分,主要为β-蒎烯(相对峰面积50.41%),水芹烯(13.42%),柠檬烯(6.96%),3-蒈烯(4.92%)和α-蒎烯(3.77%);从池杉中鉴定出37种成分,主要为β-蒎烯(40.18%),水芹烯(12.01%),柠檬烯(8.10%),石竹烯(5.77%)和顺-3-己烯醇(5.30%);从落羽杉中鉴定出28种成分,主要为β-蒎烯(42.97%),柠檬烯(30.53%),水芹烯(6.54%),莰烯(3.44%)和石竹烯(2.46%)。展开更多
In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T.mucronatum was identified by SRAP(Sequence-related Amplified Polymorphism) markers.Firstly,48 SRAP primer combinations were random...In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T.mucronatum was identified by SRAP(Sequence-related Amplified Polymorphism) markers.Firstly,48 SRAP primer combinations were randomly selected and polymorphism primers between two parents(T.distichum and T.mucronatum) were selected.As a result,all primers amplifid clear bands,and 29 primers had polymorphism bands of two parents,accounted for 60.4% of all primers,and 14 primer combinations with specific bands both two samples.Authenticity of 4 progenies from T.distichum and T.mucronatum was identified by 12 polymorphism primer combinations selected from above 29 polymorphism primer combinations.The results showed that 4 progenies were all identified to be true hybrids owing to their specific bands from male parent.Among the primer combinations,Me9+Em10 produced specific bands of both male parent and female parent in every progeny,that is to say,with this one primer combination alone,the status of 4 progenies can be identified.Efficiency of molecule identification were much improved.The results of hybrids identification provided a solid evidence for the hybrids further study and application.The experimental results also proved that SRAP molecular marker technology can be effectively applied in the researches on Taxodium.展开更多
文摘This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the effects of NAA,6-BA,IBA and KT on the induction of differentiation,proliferation and rooting were evaluated on MS or 1/2 MS medium. The results showed that Zhongshanshan can be proliferated via tissue culture. The combined use of NAA and 6-BA in MS medium greatly promoted the differentiation of Zhongshanshan explants,and the optimal medium was MS ＋ 0. 2 mg/L NAA ＋ 0. 4 mg/L6-BA. The optimal medium for the proliferation of differentiated buds was MS ＋ 0. 3 mg/L NAA ＋ 0. 4 mg/L 6-BA,on which the proliferation rate was up to 3. 8. 1/2 MS medium was more conducive than MS medium to the induction of rooting. The optimal medium for the rooting of tissue-cultured Zhongshanshan shoots was 1/2 MS ＋ 0. 3 mg/L IBA ＋ 0. 2 mg/L NAA.
文摘Saltwater intrusion is a major cause of coastal erosion. New hybrid bald cypress species have been shown to exhibit salinity tolerance compared to native bald cypress species. Planting these hybrids, or Nanjing Bot Garden genotype (NBG), trees where their roots can stabilize soil and preserve land may reduce coastal erosion in areas subjected to saltwater intrusion. The purpose of this experiment was to determine whether or not selected bald cypress NBG genotype tree species survive better than native bald cypress trees in areas inundated with saline water. Leaves were harvested, dried and analyzed for their elemental concentration. NBG genotype trees excluded more sodium than native bald cypress trees. NBG genotype tree roots were also less effected by brackish water. Finally, the growth of the NBG genotype trees and the control were measured. All NBG genotype trees tested showed growth while the control trees maintained little or no growth, and a decrease in tree height in sodic soil. If these NBG genotype trees are subjected to a surge of brackish water, NBG genotype bald cypress trees may be used to stabilize soil that may be otherwise subject to coastal erosion.
文摘采用室内盆栽方式,以两年生落羽杉属杂交树种"中山杉406"(Taxodium‘Zhongshanshan 406’)为试验材料,设置土壤含水量为土壤田间持水量的70%(对照)、渍水(水面与土面持平)、淹水10 cm 3个处理,研究中山杉406的生物量,N、P等营养元素含量,以及超氧化物歧化酶(SOD)活性等生长与生理指标的变化。结果表明,与对照相比,渍水与淹水能显著增加中山杉406的地茎,降低植株的根冠比,而对植株的株高、鲜质量、株高增长率与鲜质量增长率无显著影响。淹水处理后,中山杉406根部P、K、Na、Mg含量显著高于对照。中山杉406叶片的叶绿素含量、SOD活性、过氧化物酶(POD)活性、丙二醛(MDA)含量以及根系活力在对照、渍水、淹水3个处理间无显著差异。此外,淹水处理中山杉406出现皮孔肥大以及长出气生根等对淹水的适应特征。可见,中山杉406具有良好的耐水湿特性,可应用于湿地的恢复与构建。
文摘为了探究水杉(Metasequoia glyptostroboides Hu et Cheng)、池杉(Taxodium ascendens Brongn.)和落羽杉(T.distichum(Linn.)Rich.)挥发性有机化合物(VOCs)的差异,从植物挥发性有机化合物角度为其园林配置提供理论依据,试验采用动态顶空气体循环采集法和热脱附气质联用技术(TDS-GC-MS)对3种杉科植物挥发物进行成分分析。结果表明:萜烯类化合物是3种杉科植物挥发性有机化合物的主要成分。其中从水杉中鉴定出26种成分,主要为β-蒎烯(相对峰面积50.41%),水芹烯(13.42%),柠檬烯(6.96%),3-蒈烯(4.92%)和α-蒎烯(3.77%);从池杉中鉴定出37种成分,主要为β-蒎烯(40.18%),水芹烯(12.01%),柠檬烯(8.10%),石竹烯(5.77%)和顺-3-己烯醇(5.30%);从落羽杉中鉴定出28种成分,主要为β-蒎烯(42.97%),柠檬烯(30.53%),水芹烯(6.54%),莰烯(3.44%)和石竹烯(2.46%)。
文摘In this paper,the authenticity of 4 reciprocal progenies from Taxodium distichum and T.mucronatum was identified by SRAP(Sequence-related Amplified Polymorphism) markers.Firstly,48 SRAP primer combinations were randomly selected and polymorphism primers between two parents(T.distichum and T.mucronatum) were selected.As a result,all primers amplifid clear bands,and 29 primers had polymorphism bands of two parents,accounted for 60.4% of all primers,and 14 primer combinations with specific bands both two samples.Authenticity of 4 progenies from T.distichum and T.mucronatum was identified by 12 polymorphism primer combinations selected from above 29 polymorphism primer combinations.The results showed that 4 progenies were all identified to be true hybrids owing to their specific bands from male parent.Among the primer combinations,Me9+Em10 produced specific bands of both male parent and female parent in every progeny,that is to say,with this one primer combination alone,the status of 4 progenies can be identified.Efficiency of molecule identification were much improved.The results of hybrids identification provided a solid evidence for the hybrids further study and application.The experimental results also proved that SRAP molecular marker technology can be effectively applied in the researches on Taxodium.
