This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the ...This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the effects of NAA,6-BA,IBA and KT on the induction of differentiation,proliferation and rooting were evaluated on MS or 1/2 MS medium. The results showed that Zhongshanshan can be proliferated via tissue culture. The combined use of NAA and 6-BA in MS medium greatly promoted the differentiation of Zhongshanshan explants,and the optimal medium was MS ＋ 0. 2 mg/L NAA ＋ 0. 4 mg/L6-BA. The optimal medium for the proliferation of differentiated buds was MS ＋ 0. 3 mg/L NAA ＋ 0. 4 mg/L 6-BA,on which the proliferation rate was up to 3. 8. 1/2 MS medium was more conducive than MS medium to the induction of rooting. The optimal medium for the rooting of tissue-cultured Zhongshanshan shoots was 1/2 MS ＋ 0. 3 mg/L IBA ＋ 0. 2 mg/L NAA.展开更多
采用室内盆栽方式,以两年生落羽杉属杂交树种"中山杉406"(Taxodium‘Zhongshanshan 406’)为试验材料,设置土壤含水量为土壤田间持水量的70%(对照)、渍水(水面与土面持平)、淹水10 cm 3个处理,研究中山杉406的生物量,N、P等...采用室内盆栽方式,以两年生落羽杉属杂交树种"中山杉406"(Taxodium‘Zhongshanshan 406’)为试验材料,设置土壤含水量为土壤田间持水量的70%(对照)、渍水(水面与土面持平)、淹水10 cm 3个处理,研究中山杉406的生物量,N、P等营养元素含量,以及超氧化物歧化酶(SOD)活性等生长与生理指标的变化。结果表明,与对照相比,渍水与淹水能显著增加中山杉406的地茎,降低植株的根冠比,而对植株的株高、鲜质量、株高增长率与鲜质量增长率无显著影响。淹水处理后,中山杉406根部P、K、Na、Mg含量显著高于对照。中山杉406叶片的叶绿素含量、SOD活性、过氧化物酶(POD)活性、丙二醛(MDA)含量以及根系活力在对照、渍水、淹水3个处理间无显著差异。此外,淹水处理中山杉406出现皮孔肥大以及长出气生根等对淹水的适应特征。可见,中山杉406具有良好的耐水湿特性,可应用于湿地的恢复与构建。展开更多
文摘This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the effects of NAA,6-BA,IBA and KT on the induction of differentiation,proliferation and rooting were evaluated on MS or 1/2 MS medium. The results showed that Zhongshanshan can be proliferated via tissue culture. The combined use of NAA and 6-BA in MS medium greatly promoted the differentiation of Zhongshanshan explants,and the optimal medium was MS ＋ 0. 2 mg/L NAA ＋ 0. 4 mg/L6-BA. The optimal medium for the proliferation of differentiated buds was MS ＋ 0. 3 mg/L NAA ＋ 0. 4 mg/L 6-BA,on which the proliferation rate was up to 3. 8. 1/2 MS medium was more conducive than MS medium to the induction of rooting. The optimal medium for the rooting of tissue-cultured Zhongshanshan shoots was 1/2 MS ＋ 0. 3 mg/L IBA ＋ 0. 2 mg/L NAA.
文摘采用室内盆栽方式,以两年生落羽杉属杂交树种"中山杉406"(Taxodium‘Zhongshanshan 406’)为试验材料,设置土壤含水量为土壤田间持水量的70%(对照)、渍水(水面与土面持平)、淹水10 cm 3个处理,研究中山杉406的生物量,N、P等营养元素含量,以及超氧化物歧化酶(SOD)活性等生长与生理指标的变化。结果表明,与对照相比,渍水与淹水能显著增加中山杉406的地茎,降低植株的根冠比,而对植株的株高、鲜质量、株高增长率与鲜质量增长率无显著影响。淹水处理后,中山杉406根部P、K、Na、Mg含量显著高于对照。中山杉406叶片的叶绿素含量、SOD活性、过氧化物酶(POD)活性、丙二醛(MDA)含量以及根系活力在对照、渍水、淹水3个处理间无显著差异。此外,淹水处理中山杉406出现皮孔肥大以及长出气生根等对淹水的适应特征。可见,中山杉406具有良好的耐水湿特性,可应用于湿地的恢复与构建。
