This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the ...This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the effects of NAA,6-BA,IBA and KT on the induction of differentiation,proliferation and rooting were evaluated on MS or 1/2 MS medium. The results showed that Zhongshanshan can be proliferated via tissue culture. The combined use of NAA and 6-BA in MS medium greatly promoted the differentiation of Zhongshanshan explants,and the optimal medium was MS ＋ 0. 2 mg/L NAA ＋ 0. 4 mg/L6-BA. The optimal medium for the proliferation of differentiated buds was MS ＋ 0. 3 mg/L NAA ＋ 0. 4 mg/L 6-BA,on which the proliferation rate was up to 3. 8. 1/2 MS medium was more conducive than MS medium to the induction of rooting. The optimal medium for the rooting of tissue-cultured Zhongshanshan shoots was 1/2 MS ＋ 0. 3 mg/L IBA ＋ 0. 2 mg/L NAA.展开更多
Saltwater intrusion is a major cause of coastal erosion. New hybrid bald cypress species have been shown to exhibit salinity tolerance compared to native bald cypress species. Planting these hybrids, or Nanjing Bot Ga...Saltwater intrusion is a major cause of coastal erosion. New hybrid bald cypress species have been shown to exhibit salinity tolerance compared to native bald cypress species. Planting these hybrids, or Nanjing Bot Garden genotype (NBG), trees where their roots can stabilize soil and preserve land may reduce coastal erosion in areas subjected to saltwater intrusion. The purpose of this experiment was to determine whether or not selected bald cypress NBG genotype tree species survive better than native bald cypress trees in areas inundated with saline water. Leaves were harvested, dried and analyzed for their elemental concentration. NBG genotype trees excluded more sodium than native bald cypress trees. NBG genotype tree roots were also less effected by brackish water. Finally, the growth of the NBG genotype trees and the control were measured. All NBG genotype trees tested showed growth while the control trees maintained little or no growth, and a decrease in tree height in sodic soil. If these NBG genotype trees are subjected to a surge of brackish water, NBG genotype bald cypress trees may be used to stabilize soil that may be otherwise subject to coastal erosion.展开更多
文摘This study was carried out to optimize the culture media for the micropropagation of Taxodium hybrid Zhongshanshan（ T. distichum × T. mucronatum）.Using the tender stems of Zhongshanshan 301 as the explants,the effects of NAA,6-BA,IBA and KT on the induction of differentiation,proliferation and rooting were evaluated on MS or 1/2 MS medium. The results showed that Zhongshanshan can be proliferated via tissue culture. The combined use of NAA and 6-BA in MS medium greatly promoted the differentiation of Zhongshanshan explants,and the optimal medium was MS ＋ 0. 2 mg/L NAA ＋ 0. 4 mg/L6-BA. The optimal medium for the proliferation of differentiated buds was MS ＋ 0. 3 mg/L NAA ＋ 0. 4 mg/L 6-BA,on which the proliferation rate was up to 3. 8. 1/2 MS medium was more conducive than MS medium to the induction of rooting. The optimal medium for the rooting of tissue-cultured Zhongshanshan shoots was 1/2 MS ＋ 0. 3 mg/L IBA ＋ 0. 2 mg/L NAA.
文摘Saltwater intrusion is a major cause of coastal erosion. New hybrid bald cypress species have been shown to exhibit salinity tolerance compared to native bald cypress species. Planting these hybrids, or Nanjing Bot Garden genotype (NBG), trees where their roots can stabilize soil and preserve land may reduce coastal erosion in areas subjected to saltwater intrusion. The purpose of this experiment was to determine whether or not selected bald cypress NBG genotype tree species survive better than native bald cypress trees in areas inundated with saline water. Leaves were harvested, dried and analyzed for their elemental concentration. NBG genotype trees excluded more sodium than native bald cypress trees. NBG genotype tree roots were also less effected by brackish water. Finally, the growth of the NBG genotype trees and the control were measured. All NBG genotype trees tested showed growth while the control trees maintained little or no growth, and a decrease in tree height in sodic soil. If these NBG genotype trees are subjected to a surge of brackish water, NBG genotype bald cypress trees may be used to stabilize soil that may be otherwise subject to coastal erosion.
