The seed of \%Taxus chinensis \%(Pilger) Rehd.\ has a long postmature duration only with the germination of 29.5%～37.5% after 40～70 d when its dormancy is relieved. The low limit of mean temperature for shooting is...The seed of \%Taxus chinensis \%(Pilger) Rehd.\ has a long postmature duration only with the germination of 29.5%～37.5% after 40～70 d when its dormancy is relieved. The low limit of mean temperature for shooting is 15℃, the mean height of 1\|year\|old and 2\|years\|old seedlings is 8.12 cm and 16.10 cm respectively, mean basal diameter of 2\|years\|old seedling is 0.19 cm. Soft\|wood + 50 mg/kg ABT + sandy bed in spring and hard\|wood + 100 mg/kg NAA + sandy bed in autumn are the best treatments for cutting propagation, the survival rate of afforestation may reach 100%. Their mean height is 70.48 cm, basal diameter is 1.01 cm, and branch is 40～50 cm in second year for plantation.展开更多
The embryo culture of Taxus chinensis (Pilger) Rehd. and T. brevifolia Nutt. in vitro can shorten the dormancy period with early germinating, germination percentage is above 98% after cultured thirty days. The surviva...The embryo culture of Taxus chinensis (Pilger) Rehd. and T. brevifolia Nutt. in vitro can shorten the dormancy period with early germinating, germination percentage is above 98% after cultured thirty days. The survival rate of the seedlings increased when the seeds were stored in low temperature, it could be increased from 49% to 70% after treated four months. The seedling′s growth can be improved if reduce the main inorganic element content of the medium. Germination character of two \%Taxus \%L. species are similar.展开更多
Taxol content from the branches and leaves of Taxus chinensis var. mairei (Lemeé et Lévl.) Cheng et L. K. Fu and its cell cultures was analyzed by using Nova-Pak C-{18}column and improving the proportion of ...Taxol content from the branches and leaves of Taxus chinensis var. mairei (Lemeé et Lévl.) Cheng et L. K. Fu and its cell cultures was analyzed by using Nova-Pak C-{18}column and improving the proportion of mobile phase (methanol/acetonitrile/water) and modifing column temperature at a wavelength of 227 nm. The improved HPLC method is celerity, nicety and delicacy. The lowest detectable quantity of taxol could reach 0.005 μg.展开更多
文摘The seed of \%Taxus chinensis \%(Pilger) Rehd.\ has a long postmature duration only with the germination of 29.5%～37.5% after 40～70 d when its dormancy is relieved. The low limit of mean temperature for shooting is 15℃, the mean height of 1\|year\|old and 2\|years\|old seedlings is 8.12 cm and 16.10 cm respectively, mean basal diameter of 2\|years\|old seedling is 0.19 cm. Soft\|wood + 50 mg/kg ABT + sandy bed in spring and hard\|wood + 100 mg/kg NAA + sandy bed in autumn are the best treatments for cutting propagation, the survival rate of afforestation may reach 100%. Their mean height is 70.48 cm, basal diameter is 1.01 cm, and branch is 40～50 cm in second year for plantation.
文摘The embryo culture of Taxus chinensis (Pilger) Rehd. and T. brevifolia Nutt. in vitro can shorten the dormancy period with early germinating, germination percentage is above 98% after cultured thirty days. The survival rate of the seedlings increased when the seeds were stored in low temperature, it could be increased from 49% to 70% after treated four months. The seedling′s growth can be improved if reduce the main inorganic element content of the medium. Germination character of two \%Taxus \%L. species are similar.
文摘Taxol content from the branches and leaves of Taxus chinensis var. mairei (Lemeé et Lévl.) Cheng et L. K. Fu and its cell cultures was analyzed by using Nova-Pak C-{18}column and improving the proportion of mobile phase (methanol/acetonitrile/water) and modifing column temperature at a wavelength of 227 nm. The improved HPLC method is celerity, nicety and delicacy. The lowest detectable quantity of taxol could reach 0.005 μg.
