急性非淋巴细胞白血病IgH、TCRr基因重排的检测

研究急性非淋巴细胞白血病（ANLL）基因分型和微小残留病（MRD）的检测方法，选择IgH、TCRr基因重排标记．用PCR技术，对31例ANLL患者进行检测．结果：IgH阳性12．9％（4／31），TCRr阳性6．45％（2／31），表明ANLL中存在IrH和TCRr基因重排．对MRD的研究有一定的临床意义．

多聚酶链反应检测白血病淋巴瘤TCRr基因重排的初探

现代医学分子生物学的发展证实,急性淋巴细胞白血病(ALL)和淋巴瘤等淋巴系恶性增殖性疾病,存在着特异的免疫球蛋白基因或T细胞抗原受体(TCR)基因的重排。用多聚酶链反应(PCR)将重排的基因片段放大100万倍,由此可检测出体内占细胞总数1/100000的恶性细胞。

溴介导法标记荧光引物做彩色PCR分析白血病TCRr基因重排

采用溴介导法标记引物，做彩色ＰＣＲ分析２９例淋巴系统白血病Ｔ细胞受体ｒ（ＴＣＲｒ）基因的单克隆基因重排特征，取得进展。

急性非淋巴细胞性白血病免疫球蛋白和T细胞受体基因重排的研究及其意义

采用PCR法对25例不同时期急性非淋巴细胞性白血病（ANLL）患者免疫球蛋白重链（IgH）和T细胞受体γ链（TcRr）基因重排进行了研究。结果显示，3例（12．0％）发生IgH基因重排，3例（12．0％）出现TcRr基因重排，其中1例同时出现IgH和TcRr基因重排，对这种ANLL中序列失真现象的机制及其在白血病基因分型及检测微小残留疾病中的意义进行讨论。

淋巴细胞恶性增殖病基因重排及其意义

目的：对淋巴细胞恶性增殖病进行研究。方法：应用多聚酶链反应－聚丙烯酰胺凝胶电泳（ＰＣＲ－ＰＡＧＥ）和银染色法，对３０７ 例淋巴细胞恶性增殖病进行了免疫球蛋白重链基因（ＩＧＨ），Ｔ细胞受体ｒ链基因（ＴＣＲｒ）的克隆性重排进行检测。结果：ＩＧＨ １６０ 例（５２．１％ ），ＴＣＲｒ ５８ 例（１８８％ ），双阳性２２ 例（７．１％ ），阴性６７ 例（２１．８％ ）。非霍奇金淋巴瘤（ＮＨＬ）与ＮＨＬ白血病的基因重排分布相似；霍奇金淋巴瘤（ＨＤ）明显较低；急性淋巴细胞性白血病（ＡＬＬ）及多发性骨髓瘤（ＭＭ）进展期基因重排高达９５％ ，９０％ ；缓解期６０％ ，４５％ ；慢性淋巴细胞性白血病（ＣＬＬ）重排率低；急性粒细胞性白血病（ＡＭＬ）及慢性粒细胞性白血病（ＣＭＬ）为对照，它们也有少量淋巴细胞基因重排。病理学型别以不能分型者，原发灶以鼻及咽淋巴环者之ＴＣＲｒ 重排明显增高，且与恶性程度呈正相关。并可以此重排来检测微小病灶（ＭＲＤ）之存在。结论：可用此指导化疗。

15例急性淋巴细胞性白血病微小残留病追踪检测结果分析

为探讨残留白血病细胞的存在与白血病患儿长期生存之间的关系,我们用非同位素的PCR-RNA转录本分子杂交法,选用T细胞抗原受体γ链(TCR_γ)重排基因为标志,检测了20例急性淋巴细胞白血病(ALL)患儿,并对其中具有TCR_γ重排基因的15例患儿进行了追踪检测,结果显示:化疗后早期(<5月)大多数患儿都能检测到残留白血病细胞,长期缓解者微小残留病检测为阴性。结果表明,当临床达到完全缓解时,对每1例患者的残留白血病细胞进行监测是必要的。

Drawing mechanisms for top coal in longwall top coal caving(LTCC):A review of two decades of literature

This review details the state of the art in research on top coal drawing mechanisms in Longwall top coal caving(LTCC)by examining the relevant literature over the last two decades.It startswith an introduction of the brief history and basic procedures of LTCC.The framework of research on the drawing mechanism,basic concepts,and some theoretical models of LTCC are detailed in sect.research framework of top coal drawingmechanism.The authors note that theTop coal drawbody(TCD),Top coal boundary(TCB)and Top coal recovery ratio(TCRR)are key factors in the drawingmechanism.TheBody-boundary-ratio(BBR)research system has been the classic framework for research over the last 20 years.The modified Bergmark-Roos model,which considers the effects of the supporting rear canopy,flowing velocity of top coal,and its shape factor,is optimal for characterizing the TCD.A 3Dmodel to describe the TCB that considers the thicknesses of the coal seam and roof strata is reviewed.In sect.physical testing and numerical simulation,the physical tests and numerical simulations in the literature are classified for ease of bibliographical review,and classic conclusions regarding the drawing mechanism of top coal are presented and discussedwith elaborate illustrations and descriptions.The deflection of the TCDis noted,and is caused by the shape of the rear canopy.The inclined coal seam always induces a largerTCD,and a deflection in theTCDhas also been observed in it.The effects of the drawing sequence and drawing interval on the TCRR are reviewed,where a long drawing interval is found to lead to significant loss of top coal.Its flowing behavior and velocity distribution are also presented.Sect.practical applications of drawingmechanisms forLTCCmines 4 summarizes over 10 cases where the TCRRof LTCCmines improved due to the guidance of the drawing mechanism.The final section provides a summary of the work here and some open questions.Prospective investigations are highlighted to give researchers guidance on promising issues in future research on LTCC.

FAB分类慢性淋巴系白血病临床及细胞形态学和生物学特征研究

按1989年FAB对慢性淋巴系白血病分类建议，根据临床、细胞／组织形态学、免疫表型及PCR法IgH与TCRr基因重排分析，血尿免疫球蛋白定量研究，对20例患者（B细胞白血病16例，T细胞白血病4例）进行研究分析。并就B－CLL诊断标准、形态学与生物学特点，疾病的转归，B－PLL、WM及T－LGLL、NK－LGLL的诊断要点进行讨论。对T-白血病／淋巴瘤是否亦应列入分类提出看法，强调了形态学、免疫学及分子遗传学三维诊断对该系统疾病研究的重要性。