Polyphenols,including phenolic acids,flavonoids,and procyanidins,are abundant in food and beverage derived from plants.Tea(Camellia sinensis)is particularly rich in polyphenols(e.g.,catechins,theaflavins,thearubigins,...Polyphenols,including phenolic acids,flavonoids,and procyanidins,are abundant in food and beverage derived from plants.Tea(Camellia sinensis)is particularly rich in polyphenols(e.g.,catechins,theaflavins,thearubigins,gallic acid,and flavonols),which are thought to contribute to the health benefits of tea.High intake of tea polyphenols has been described to prevent and/or attenuate a variety of chronic pathological conditions like cardiovascular diseases,neurodegenerative diseases,diabetes,and cancer.This review focuses on established antioxidant and anti-inflammatory properties of tea polyphenols and underlying mechanisms of their involvement in inflammatory bowel diseases(IBD).Tea polyphenols act as efficient antioxidants by inducing an endogenous antioxidant defense system and maintaining intracellular redox homeostasis.Tea polyphenols also regulate signaling pathways such as nuclear factor-κB,activator protein 1,signal transducer and activator of transcriptions,and nuclear factor E2-related factor 2,which are associated with IBD development.Accumulating pieces of evidence have indicated that tea polyphenols enhance epithelial barrier function and improve gut microbial dysbiosis,contributing to the management of inflammatory colitis.Therefore,this study suggests that supplementation of tea polyphenols could prevent inflammatory conditions and improve the outcome of patients with IBD.展开更多
[ Objedlve] To investigate the effects of tea polyphenols (TP) and vitamin E (VE ) on growth performance and antioxidant performance of broilers in the routine breeding conditions. [ Method] A total of 270 broiler...[ Objedlve] To investigate the effects of tea polyphenols (TP) and vitamin E (VE ) on growth performance and antioxidant performance of broilers in the routine breeding conditions. [ Method] A total of 270 broilers at 1 day old were fed on the basic diets supplemented with TP and VE at different proportions. The malondialdehyde (MDA) content, total antioxidant capacity (T-AOC) as well as activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. The whole weight gain and feed conversion rate were also determined. [ Result] The broilers fed on TP and VE had remarkably higher serum activities of SOD and GSH-Px, stronger T-AOC and lower MDA content than the control ones. The best effects were achieved when the supplementary dosage of -rP and VE was respectively 60 and 80 mg/kg · Diet, because the experi- mental broilers had significantly higher antioxidant indices than the control ones ( P 〈 0.01 ). Additionally, the boilers at the age of 21 or 42 days old had significantly higher body weight and lower feed conversion rate than the control ones ( P 〈 0.01 ). [ Conclusion] The study provides a theoreti- cal basis for the applications of TP and VE in animal production.展开更多
Purpose: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2O2 and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to...Purpose: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2O2 and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to testing their antioxidative abilities. Methods: The experiments were performed at 37℃ with final concentration of 2.5mg/ml protein, 0.1 mM metal ions, 0.3 mM EDTA and 1.0 mM H2O2. Then the TP was added to the solution with CuSO4 and H2O2, after 5 or 24 hours, the crystallins were analysed with SDS-PAGE and IEF.Results : There were marked oxidative modifications of lens protein in H2O2 and copper without EDTA. In SDS-PAGE patterns, we found an increase in those species above of bands higher than 30 kD and some diffuse bands from 30 to 17 kD after 5 hours. In IEF patterns, there were a general increase on acidity with loss of the more basic species. When the TP was added, there was not any difference with control group.Conclusion: The results indicate that exposure of water-soluble展开更多
[Objectives]To explore the change rule of polyphenol content and antioxidant activity of coarse old leaves of Yingshan Yunwu Tea in the process of human digestion.[Methods]The coarse and old leaves of Yunwu tea in Yin...[Objectives]To explore the change rule of polyphenol content and antioxidant activity of coarse old leaves of Yingshan Yunwu Tea in the process of human digestion.[Methods]The coarse and old leaves of Yunwu tea in Yingshan,Huanggang,Hubei Province were selected as the research object,and their digestion in vitro was simulated.The total polyphenol content was determined by Folin-phenol reagent colorimetric method,and the DPPH radical scavenging activity and total antioxidant activity were determined.[Results]After simulated gastrointestinal digestion in vitro,the polyphenol content and antioxidant activity of coarse old leaf tea soup showed a downward trend.After gastrointestinal digestion,the polyphenol content in tea infusion separately decreased by 31.8%and 8.5%;the scavenging rate of DPPH free radical was 97%before digestion,decreased to 92%after gastric digestion and 65%after intestinal digestion,which decreased by 5%and 27%,respectively;after gastrointestinal digestion,the total antioxidant capacity of tea soup decreased by 4.7%and 3.1%,respectively.[Conclusions]This study provided a reference for the development and application of coarse old leaves of Yingshan Yunwu tea,and provided a reference for the nutritional value evaluation and comprehensive utilization of coarse old leaves,so as to make the best use of coarse tea leaves and reduce the waste of resources.展开更多
Tea trees have a long history of cultivation and utilization.People in many countries have the habit of drinking tea and choosing green tea,oolong tea,or black tea according to different regions and personal tastes.Te...Tea trees have a long history of cultivation and utilization.People in many countries have the habit of drinking tea and choosing green tea,oolong tea,or black tea according to different regions and personal tastes.Tea polyphe nols are a general term for polyphenol co mpounds in tea,and has been shown to have good effects on antioxidant,anti-inflammatory,cancer prevention and regulation of lipid metabolism.Tea polyphenols have been widely used as antioxidants in disease treatment and animal husbandry,but their specific mechanism of action needs to be further clarified and revealed.This review focuses on the definition,classification,antioxidant activity and the regulation of signaling pathways of tea polyphenols.This paper also aims to examine the application of tea polyphenols in human and animal health,providing a scientific basis for this application in addition to proposing future directions for the development of this resource.展开更多
The in vitro oxidative hemolysis of human red blood cells (RBC) was used as a model to study the free radical induced damage of biological membranes and the inhibitory effect of natural antioxidants. The hemolysis was...The in vitro oxidative hemolysis of human red blood cells (RBC) was used as a model to study the free radical induced damage of biological membranes and the inhibitory effect of natural antioxidants. The hemolysis was induced by a water-soluble free radical initiator 2,2’-azo(2-asmidinopropane)dihydrochloride (AAPH) and inhibited by the principal polyphenolic components extracted from green tea leaves, i.e. (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicat-echin gallate (ECG), (-)-epigallocatechin gallate (EGCG) and gallic acid (GA). Addition of AAPH at 37℃ caused fast hemolysis after a short period of inhibition period, while addition of the green tea polyphenols efficiently suppressed the hemolysis in the activity sequence of EGCG 】 EGC】 ECG ≈EC】GA, demonstrating that these green tea polyphenols are effective antioxidants which could protect biological membranes from free radical induced oxidative damage.展开更多
This study was conducted to evaluate the effects of ageing corn levels(stored for 4 years)with or without the supplementation of tea polyphenols(TPP)on the performance,egg quality and antioxidant status of laying hens...This study was conducted to evaluate the effects of ageing corn levels(stored for 4 years)with or without the supplementation of tea polyphenols(TPP)on the performance,egg quality and antioxidant status of laying hens.A total of 288 Lohmann commercial laying hens(63-week-old)were used under a 2×4 factorial arrangement with 4 levels of dietary ageing corn(0%,25%,50%,or 100%)and 2 levels of TPP(0 and 600 mg/kg)for 8 wk.Dietary ageing corn linearly decreased(P<0.05)the egg production,serum total antioxidant capacity(T-AOC),liver glutathione peroxidase(GSH-Px)of laying hens,yolk index,yolk colour,1,1-diphenyl-2-picrylhydrazyl(DPPH)value and the reducing power value of egg yolk,but it linearly increased(P<0.05)the feed conversion rate,ovary malondialdehyde(MDA)content of laying hens,and the protein carbonyl content of egg yolk.Tea polyphenol supplementation increased(P<0.05)the serum T-AOC,serum superoxide dismutase(SOD),liver SOD,liver GSH-Px,ovary SOD,GSH-Px,the expression of antioxidant-related genes of laying hens,albumen height,Haugh unit,DPPH value and the majority free amino acids of egg yolk,but it decreased(P<0.05)the serum MDA content of laying hens,MDA and protein carbonyl of egg yolk.In conclusion,the ageing corn significantly reduced the perfor-mance,egg quality,antioxidant status and egg antioxidant capacity of laying hens,while TPP supple-mentation partially counteracted the adverse effects,especially antioxidant status and egg antioxidant capacity of laying hens.展开更多
Extracts of 40 green, black or oolong high quality tea samples from China, Japan or India among others, have been prepared according to the manufacturer instructions and assayed for total polyphenols content using the...Extracts of 40 green, black or oolong high quality tea samples from China, Japan or India among others, have been prepared according to the manufacturer instructions and assayed for total polyphenols content using the Folin-Ciocalteu phenol reagent. In addition, the antioxidant activity was assessed by the CUPRAC method and the free-radical scavenging activity was determined using the antioxidant-promoting decay of the stable free-radical DPPH. Caffeine and the most important catechins were identified using RP-HPLC previously validated method. Results from this study suggest that teas, even though they content similar amounts of caffeine, differ considerably in polyphenolic content and therefore in antioxidant and free-radical scavenging activities, depending on the part of the plant used and the process applied to the material for the preparation of the final tea presentation. Correlations among Folin-Ciocalteau’s reactivity of tea samples, the neocuproine reactivity, the DPPH decolourization and the HPLC analysis suggest that the antioxidant activity is due essentially to polyphenolic compounds present in teas, mainly EGCG. The best extraction method of tea powder was the one indicated by dealers. In general, the richest samples belong to green teas and the poorest samples belong to the black teas.展开更多
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.NRF-2020R1F1A1073595 and 2021R1A2C2006745)。
文摘Polyphenols,including phenolic acids,flavonoids,and procyanidins,are abundant in food and beverage derived from plants.Tea(Camellia sinensis)is particularly rich in polyphenols(e.g.,catechins,theaflavins,thearubigins,gallic acid,and flavonols),which are thought to contribute to the health benefits of tea.High intake of tea polyphenols has been described to prevent and/or attenuate a variety of chronic pathological conditions like cardiovascular diseases,neurodegenerative diseases,diabetes,and cancer.This review focuses on established antioxidant and anti-inflammatory properties of tea polyphenols and underlying mechanisms of their involvement in inflammatory bowel diseases(IBD).Tea polyphenols act as efficient antioxidants by inducing an endogenous antioxidant defense system and maintaining intracellular redox homeostasis.Tea polyphenols also regulate signaling pathways such as nuclear factor-κB,activator protein 1,signal transducer and activator of transcriptions,and nuclear factor E2-related factor 2,which are associated with IBD development.Accumulating pieces of evidence have indicated that tea polyphenols enhance epithelial barrier function and improve gut microbial dysbiosis,contributing to the management of inflammatory colitis.Therefore,this study suggests that supplementation of tea polyphenols could prevent inflammatory conditions and improve the outcome of patients with IBD.
基金supported by Hebei Education Department(2010252)
文摘[ Objedlve] To investigate the effects of tea polyphenols (TP) and vitamin E (VE ) on growth performance and antioxidant performance of broilers in the routine breeding conditions. [ Method] A total of 270 broilers at 1 day old were fed on the basic diets supplemented with TP and VE at different proportions. The malondialdehyde (MDA) content, total antioxidant capacity (T-AOC) as well as activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. The whole weight gain and feed conversion rate were also determined. [ Result] The broilers fed on TP and VE had remarkably higher serum activities of SOD and GSH-Px, stronger T-AOC and lower MDA content than the control ones. The best effects were achieved when the supplementary dosage of -rP and VE was respectively 60 and 80 mg/kg · Diet, because the experi- mental broilers had significantly higher antioxidant indices than the control ones ( P 〈 0.01 ). Additionally, the boilers at the age of 21 or 42 days old had significantly higher body weight and lower feed conversion rate than the control ones ( P 〈 0.01 ). [ Conclusion] The study provides a theoreti- cal basis for the applications of TP and VE in animal production.
文摘Purpose: To investigate the oxidative modification of water-soluble crystallins of human fetal lens with H2O2 and fourteen metal ions with or without EDTA. Tea-polyphenols (TP) was added to above solutions in order to testing their antioxidative abilities. Methods: The experiments were performed at 37℃ with final concentration of 2.5mg/ml protein, 0.1 mM metal ions, 0.3 mM EDTA and 1.0 mM H2O2. Then the TP was added to the solution with CuSO4 and H2O2, after 5 or 24 hours, the crystallins were analysed with SDS-PAGE and IEF.Results : There were marked oxidative modifications of lens protein in H2O2 and copper without EDTA. In SDS-PAGE patterns, we found an increase in those species above of bands higher than 30 kD and some diffuse bands from 30 to 17 kD after 5 hours. In IEF patterns, there were a general increase on acidity with loss of the more basic species. When the TP was added, there was not any difference with control group.Conclusion: The results indicate that exposure of water-soluble
基金Supported by High-level Training Project of Huanggang Normal University in 2021(202108504).
文摘[Objectives]To explore the change rule of polyphenol content and antioxidant activity of coarse old leaves of Yingshan Yunwu Tea in the process of human digestion.[Methods]The coarse and old leaves of Yunwu tea in Yingshan,Huanggang,Hubei Province were selected as the research object,and their digestion in vitro was simulated.The total polyphenol content was determined by Folin-phenol reagent colorimetric method,and the DPPH radical scavenging activity and total antioxidant activity were determined.[Results]After simulated gastrointestinal digestion in vitro,the polyphenol content and antioxidant activity of coarse old leaf tea soup showed a downward trend.After gastrointestinal digestion,the polyphenol content in tea infusion separately decreased by 31.8%and 8.5%;the scavenging rate of DPPH free radical was 97%before digestion,decreased to 92%after gastric digestion and 65%after intestinal digestion,which decreased by 5%and 27%,respectively;after gastrointestinal digestion,the total antioxidant capacity of tea soup decreased by 4.7%and 3.1%,respectively.[Conclusions]This study provided a reference for the development and application of coarse old leaves of Yingshan Yunwu tea,and provided a reference for the nutritional value evaluation and comprehensive utilization of coarse old leaves,so as to make the best use of coarse tea leaves and reduce the waste of resources.
基金financially supported by the National Key R&D Program(2018YFD0500405)the National Nature Science Foundation of China(31972582)+3 种基金the Science and Technology Projects of Changsha City(kq1801059)the STS regional key projects of Chinese Academy of Sciences(KFJ-STS-QYZD-052)the Youth Innovation Team Project of ISA,CAS(2017QNCXTD_ZCS)the Earmarked Fund for China Agriculture Research System(CARS-35)
文摘Tea trees have a long history of cultivation and utilization.People in many countries have the habit of drinking tea and choosing green tea,oolong tea,or black tea according to different regions and personal tastes.Tea polyphe nols are a general term for polyphenol co mpounds in tea,and has been shown to have good effects on antioxidant,anti-inflammatory,cancer prevention and regulation of lipid metabolism.Tea polyphenols have been widely used as antioxidants in disease treatment and animal husbandry,but their specific mechanism of action needs to be further clarified and revealed.This review focuses on the definition,classification,antioxidant activity and the regulation of signaling pathways of tea polyphenols.This paper also aims to examine the application of tea polyphenols in human and animal health,providing a scientific basis for this application in addition to proposing future directions for the development of this resource.
文摘The in vitro oxidative hemolysis of human red blood cells (RBC) was used as a model to study the free radical induced damage of biological membranes and the inhibitory effect of natural antioxidants. The hemolysis was induced by a water-soluble free radical initiator 2,2’-azo(2-asmidinopropane)dihydrochloride (AAPH) and inhibited by the principal polyphenolic components extracted from green tea leaves, i.e. (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicat-echin gallate (ECG), (-)-epigallocatechin gallate (EGCG) and gallic acid (GA). Addition of AAPH at 37℃ caused fast hemolysis after a short period of inhibition period, while addition of the green tea polyphenols efficiently suppressed the hemolysis in the activity sequence of EGCG 】 EGC】 ECG ≈EC】GA, demonstrating that these green tea polyphenols are effective antioxidants which could protect biological membranes from free radical induced oxidative damage.
基金financially supported by the National Science and Technology Project(2014BAD13B04)Sichuan Provincial Science and Technology Project(2018NZ20009)
文摘This study was conducted to evaluate the effects of ageing corn levels(stored for 4 years)with or without the supplementation of tea polyphenols(TPP)on the performance,egg quality and antioxidant status of laying hens.A total of 288 Lohmann commercial laying hens(63-week-old)were used under a 2×4 factorial arrangement with 4 levels of dietary ageing corn(0%,25%,50%,or 100%)and 2 levels of TPP(0 and 600 mg/kg)for 8 wk.Dietary ageing corn linearly decreased(P<0.05)the egg production,serum total antioxidant capacity(T-AOC),liver glutathione peroxidase(GSH-Px)of laying hens,yolk index,yolk colour,1,1-diphenyl-2-picrylhydrazyl(DPPH)value and the reducing power value of egg yolk,but it linearly increased(P<0.05)the feed conversion rate,ovary malondialdehyde(MDA)content of laying hens,and the protein carbonyl content of egg yolk.Tea polyphenol supplementation increased(P<0.05)the serum T-AOC,serum superoxide dismutase(SOD),liver SOD,liver GSH-Px,ovary SOD,GSH-Px,the expression of antioxidant-related genes of laying hens,albumen height,Haugh unit,DPPH value and the majority free amino acids of egg yolk,but it decreased(P<0.05)the serum MDA content of laying hens,MDA and protein carbonyl of egg yolk.In conclusion,the ageing corn significantly reduced the perfor-mance,egg quality,antioxidant status and egg antioxidant capacity of laying hens,while TPP supple-mentation partially counteracted the adverse effects,especially antioxidant status and egg antioxidant capacity of laying hens.
文摘Extracts of 40 green, black or oolong high quality tea samples from China, Japan or India among others, have been prepared according to the manufacturer instructions and assayed for total polyphenols content using the Folin-Ciocalteu phenol reagent. In addition, the antioxidant activity was assessed by the CUPRAC method and the free-radical scavenging activity was determined using the antioxidant-promoting decay of the stable free-radical DPPH. Caffeine and the most important catechins were identified using RP-HPLC previously validated method. Results from this study suggest that teas, even though they content similar amounts of caffeine, differ considerably in polyphenolic content and therefore in antioxidant and free-radical scavenging activities, depending on the part of the plant used and the process applied to the material for the preparation of the final tea presentation. Correlations among Folin-Ciocalteau’s reactivity of tea samples, the neocuproine reactivity, the DPPH decolourization and the HPLC analysis suggest that the antioxidant activity is due essentially to polyphenolic compounds present in teas, mainly EGCG. The best extraction method of tea powder was the one indicated by dealers. In general, the richest samples belong to green teas and the poorest samples belong to the black teas.