Cutleaf teasel (Dipsacus laciniatus L.) is an invasive plant that is spreading through natural and disturbed areas. Teasel grows for two or more years as a rosette which stays green late in the growing season and begi...Cutleaf teasel (Dipsacus laciniatus L.) is an invasive plant that is spreading through natural and disturbed areas. Teasel grows for two or more years as a rosette which stays green late in the growing season and begins growth earlier in spring than its native competitors. The purpose of this study was to find a time both seasonally and in cutleaf teasel’s life history when herbicides could be applied to decrease teasel with the least impact on the surrounding vegetation. We tested the effects of three different herbicides (glyphosate (Round-UpTM), triclopyr amine (GarlonTM), and clopyralid (LontrelTM)) on cut and uncut teasel at three different times of the year (July and October 2005, and April 2006) near Clinton Lake in Dewitt Co. Illinois. Photosynthetic measurements were taken before application to determine teasel’s susceptibility to the herbicides, and we harvested seed heads and rosettes in late October 2006. Results indicated teasel was photosynthetically active at all three application times. Cutting before herbicide application had no significant effect on the number of seeds produced or the dry weight of the rosettes. Herbicide treatment in April significantly reduced the amount of seeds produced, but there were no significant differences among the three herbicides. Clopyralid application in April significantly reduced rosette biomass, but none of the herbicides significantly affected rosette biomass at the other two times. Our studies suggest herbicide application early in the growing season may be beneficial in controlling the spread of teasel, and that mowing at the time of spraying will not increase effectiveness of the herbicide.展开更多
目的基于网络药理学方法探讨杜仲-续断药对治疗膝关节炎的药效物质基础与潜在的分子作用机制。方法通过TCMSP数据库挖掘杜仲、续断的主要化学成分,结合SwissTargetPrediction平台筛选出药物活性成分并通过Uniprot数据库进行靶点预测;通...目的基于网络药理学方法探讨杜仲-续断药对治疗膝关节炎的药效物质基础与潜在的分子作用机制。方法通过TCMSP数据库挖掘杜仲、续断的主要化学成分,结合SwissTargetPrediction平台筛选出药物活性成分并通过Uniprot数据库进行靶点预测;通过GeneCards、OMIM、DrugBank、TTD数据库检索膝关节炎主要作用靶点,利用String平台进行蛋白质交互作用(protein-protein interaction,PPI)分析,制作PPI网络图;再根据David数据库进行基因本体论(gene ontology,GO)分析和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,并通过Cytoscape3.8.0软件构建“靶点-通路”网络图。结果杜仲-续断配对调治膝关节炎的核心活性成分为β-谷甾醇、槲皮素等,重要靶点有丝裂原活化蛋白激酶1(mitogen-activated protein kinase,MAPK)抗体、肿瘤坏死因子(tumor necrosis factor,TNF)等。杜仲-续断配对治疗膝关节炎的生物学通路主要作用于TNF信号通路、磷脂酰肌醇3-激酶/蛋白激酶B(phosphatidylinositol-3-kinase/protein kinase B,PI3K/Akt)信号通路、MAPK信号通路等。结论杜仲-续断配对治疗膝关节炎具有多成分、多靶点、多通路的作用特点。展开更多
文摘Cutleaf teasel (Dipsacus laciniatus L.) is an invasive plant that is spreading through natural and disturbed areas. Teasel grows for two or more years as a rosette which stays green late in the growing season and begins growth earlier in spring than its native competitors. The purpose of this study was to find a time both seasonally and in cutleaf teasel’s life history when herbicides could be applied to decrease teasel with the least impact on the surrounding vegetation. We tested the effects of three different herbicides (glyphosate (Round-UpTM), triclopyr amine (GarlonTM), and clopyralid (LontrelTM)) on cut and uncut teasel at three different times of the year (July and October 2005, and April 2006) near Clinton Lake in Dewitt Co. Illinois. Photosynthetic measurements were taken before application to determine teasel’s susceptibility to the herbicides, and we harvested seed heads and rosettes in late October 2006. Results indicated teasel was photosynthetically active at all three application times. Cutting before herbicide application had no significant effect on the number of seeds produced or the dry weight of the rosettes. Herbicide treatment in April significantly reduced the amount of seeds produced, but there were no significant differences among the three herbicides. Clopyralid application in April significantly reduced rosette biomass, but none of the herbicides significantly affected rosette biomass at the other two times. Our studies suggest herbicide application early in the growing season may be beneficial in controlling the spread of teasel, and that mowing at the time of spraying will not increase effectiveness of the herbicide.
文摘目的基于网络药理学方法探讨杜仲-续断药对治疗膝关节炎的药效物质基础与潜在的分子作用机制。方法通过TCMSP数据库挖掘杜仲、续断的主要化学成分,结合SwissTargetPrediction平台筛选出药物活性成分并通过Uniprot数据库进行靶点预测;通过GeneCards、OMIM、DrugBank、TTD数据库检索膝关节炎主要作用靶点,利用String平台进行蛋白质交互作用(protein-protein interaction,PPI)分析,制作PPI网络图;再根据David数据库进行基因本体论(gene ontology,GO)分析和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析,并通过Cytoscape3.8.0软件构建“靶点-通路”网络图。结果杜仲-续断配对调治膝关节炎的核心活性成分为β-谷甾醇、槲皮素等,重要靶点有丝裂原活化蛋白激酶1(mitogen-activated protein kinase,MAPK)抗体、肿瘤坏死因子(tumor necrosis factor,TNF)等。杜仲-续断配对治疗膝关节炎的生物学通路主要作用于TNF信号通路、磷脂酰肌醇3-激酶/蛋白激酶B(phosphatidylinositol-3-kinase/protein kinase B,PI3K/Akt)信号通路、MAPK信号通路等。结论杜仲-续断配对治疗膝关节炎具有多成分、多靶点、多通路的作用特点。
