金属镉应答新基因TEF-1δ的致癌力鉴定

目的 前期研究已发现金属镉应答新基因TEF -1δ转染NIH3T3细胞可致其编码蛋白质表达升高而引起细胞转化。本研究的目的是对TEF 1δ基因转化NIH3T3细胞的致癌能力和致瘤性进行鉴定。方法 软琼脂集落形成试验和裸鼠成瘤实验。结果 所有 4株不同的TEF 1δ转染发生转化的NIH3T3细胞在软琼脂培养中均显示锚非依赖性生长能力 ,形成明显的细胞集落。而非转化对照细胞在软琼脂上则无生长能力。转化细胞和对照细胞分别给裸鼠皮下注射 (每组 4只 ,5组共 2 0只 ) ,1周后 ,转化细胞组的绝大部分裸鼠下背部皮下开始出现肿瘤 ,4周后所有 4种不同转化细胞株接种的裸鼠全部长出大小不等的肿瘤 ,但非转化对照细胞于第 5周后仍无一只裸鼠出现肿瘤。提示TEF 1δ转基因转化细胞属恶性转化细胞 ,具有明显的致癌能力和致瘤作用。结论 根据本研究结果及前期发现 ,可以认为新发现的TEF

基于TEF6606车载收音机模块设计

介绍了TEF6606芯片在车载收音机中的应用,根据车载供电的特点设计电路,实现了省电模式下低功耗操作;利用I^2C总线通信,控制TEF6606芯片相应的寄存器实现数字调频立体声收音功能的方法,并通过软件编程实现自动搜台、浏览选听等收音功能的多样化,满足不同的收音要求。

镉应答新基因TEF-1δ的生物学功能研究

目的 探讨镉应答新基因TEF 1δ在细胞转化和致癌过程中的重要作用。方法 应用细胞转染、WesternBlot以及细胞转化等技术与方法 ,对克隆化基因TEF 1δ的生物学功能进行研究。结果 TEF 1δcDNA以pcDNA 3 1 V5 His TOPO为表达载体所转染的CHO细胞和猴肾COS1细胞均可表达相对分子质量为 3 10 0 0的TEF 1δ编码蛋白质 ,而非转染和单纯载体转染的对照细胞则无此蛋白质表达。进一步研究表明 ,TEF 1δcDNA转染NIH3T3细胞可导致TEF 1δ蛋白质超额表达 ,并与细胞转化密切相关。结论 镉的细胞转化和致癌作用至少部分因TEF 1δ基因的高表达所致。TEF 1δ可能是一个新发现的镉应答原癌基因。

英国何以让高校教学更卓越?——追踪教学卓越与学生成果框架(TEF)的成效、反思与改进

英国高等教育正在经历一场根本性的变革,政府围绕学生的选择,研发了教学卓越与学生成果框架(TEF),旨在向学生提供更高质量、透明公开和物有所值的高等教育服务。2016~2017年是TEF的首轮预期评估,英国政府通过对此次评估结果的全面考察,系统分析了院校获奖结果、学生参与特征和用户体验反响;深刻反思了评估指标的精准性与引导性、评估过程的差异性和适切性、评估架构的监管性和服务性等价值导向;相应改进了评估指标技术和功能、评估过程方法和模式、评估架构体系和机制。基于此,英国教学卓越与学生成果框架及其评估工作将对我国高校教学评估的目的、宗旨、技术、方法和主体等方面提供有益的启示。

TEF-1δ基因真核细胞稳定表达系统的构建与鉴定

目的 :构建TEF 1δ (mousetranslationelongationfactor 1δ)的稳定表达系统。方法 :采用磷酸钙介导转染技术和G4 18细胞筛选法 ,以 pcDNA3.1/V5 His TOPO为表达载体 ,构建了TEF 1δ转基因CHO和COS7细胞系 ,WesternBlot分析与鉴定表达蛋白。结果 :在 10株转染和经G4 18反复筛选的CHO细胞系中 ,有 3株 (编码为COH pcDNA3.1 TEF 1δ # 3,# 6 ,# 14 )具有高效稳定表达的TEF 1δ编码蛋白质 (Mr约 31× 10 3 ) ,其余CHO细胞株的TEF 1δ蛋白质表达相对较弱或无表达。在 4株转染和经G4 18反复筛选的COS7细胞系中 ,4株细胞 (编码为COS7 pcCDNA3.1 TEF 1δ # 4 ,# 8,# 14和 # 17)均有高效稳定的TEF 1δ编码蛋白表达 ,相应的无转染组及载体对照组的CHO和COS7细胞均无TEF 1δ蛋白质表达。结论 :这两类TEF 1δ转基因哺乳动物细胞稳定表达系统已成功构建与鉴定 ,该表达系统的建立对于TEF 1δ这一新基因的生物学功能研究 ,尤其是镉的致癌作用与致癌机制的研究有重要应用价值。

反义翻译延伸因子TEF-1δ对氯化镉致癌性的逆转作用

背景与目的:翻译延伸因子TEF-1δ(基因文库新添编号为AF304351)是一个新发现的镉应答原癌基因。本研究之目的在于探讨反义TEF-1δ能否逆转镉转化细胞的致癌性。材料与方法:用磷酸钙介导转染法和G418细胞筛选技术,建立CdCl2转化BALB／c-3T3细胞反义TEF1δ稳定表达系统,再用软琼脂检测和裸鼠致瘤试验对这些转基因细胞的致癌性逆转情况进行鉴定。结果:CdCl2转化BALB／c-3T3细胞中反义TEF-1δ表达可逆转这些细胞的转化表型,与非转染细胞和载体转染对照细胞比较,转染反义TEF-1δ的镉转化细胞在软琼脂上所形成的锚非依赖性生长集落减少29％-44％。转染反义TEF-1δ基因的镉转化细胞可延迟裸鼠出现肿瘤的时间,而且这些肿瘤的大小显著变小,肿瘤重量平均下降54％-58％。结论:反义TEF-1δ mRNA表达可逆转镉转化细胞的致癌性;应用反义TEF-1δ阻断TEF-1δ癌基因表达对镉诱发的肿瘤可能有治疗价值。

氯化镉诱发16HBE细胞恶变过程中TEF-1δ p31异常表达的研究

背景与目的:探讨氯化镉(CdCl2)诱发人支气管上皮细胞(16HBE)恶性转化过程中不同阶段人翻译延伸因子TEF-1δ p31 mRNA表达的变化,以进一步阐明CdCl2致癌的分子机制。材料与方法:应用RT与PCR技术,以及Taqman荧光定量PCR方法,检测不同浓度CdCl2诱发16HBE恶性转化3个不同阶段细胞(转化期间细胞、转化细胞和成瘤细胞)的TEF-1δ p31 mRNA表达量的变化。结果:①相对于非转化16HBE对照细胞,CdCl2诱发恶性转化3个不同阶段细胞的TEF-1δ mRNA表达水平均显著升高(P<0.01或P<0.05),5μmol/L CdCl2处理组各阶段细胞内的TEF-1δ平均表达量分别是对照细胞的3.4、5.2和6.9倍;10μmol/L CdCl2处理组各阶段细胞内的TEF-1δ平均表达量分别是对照细胞的4.1、6.9和6.3倍;15μmol/L CdCl2处理组各阶段转化细胞内的TEF-1δ平均表达量分别是对照细胞的1.4、2.9和8.4倍。提示TEF-1δ的异常表达量与CdCl2诱发16HBE细胞恶变程度之间有正相关关系(r>0.799,P<0.01)。②16HBE细胞恶变不同阶段TEF-1δp31的异常表达水平与CdCl2的剂量相关(P=0.001)。结论:氯化镉在诱发16HBE细胞恶变过程中,存在明显的人翻译延伸因子TEF-1δ p31异常表达的现象,其表达水平与细胞恶性转化程度和CdCl2的剂量密切相关,这可能是氯化镉诱发人细胞肿瘤的重要分子致癌机制之一。

汽车收音机调谐器专用集成电路TEF6860HL的原理与应用

随着汽车工业和汽车多媒体技术的发展,汽车收音机调谐器技术也趋向于单片集成化、全球应用化、自动调试化等特点发展。本文结合飞利浦公司最新开发设计的CREST IC TEF6860HL型高性能汽车收音机处理芯片,阐述了汽车收音机调谐器的最新技术发展方向和原理应用。

Additive Main Effects and Multiplicative Interaction and Other Stability Analyses of Tef [<i>Eragrostis tef</i>(Zucc.)Trotter] Grain Yield

Tef [Eragrostis tef (Zucc.)Trotter]) is one of the most important cereal crops grown in Ethiopia. Tef production has been partly constrained by low yield and less stability of the genotypes under cultivation. Field experiments were carried out in Halaba, Loka Abaya, Bensa and Areka, South Ethiopia, from August to November, during 2016 and 2017 main cropping seasons, in order to estimate yield stability and the association between AMMI analysis and other stability parameters. Experiments were laid out in randomized complete block design with three replications using fourteen improved tef genotypes. Mean yield for Halaba, Loka Abaya2016, Loka Abaya2017, Bensa, Areka2016 and Areka2017 was 0.99, 0.45, 0.48, 1.50, 1.62 and 0.77 tons/ha, respectively. Genotypes Amarach, Boset, Simada, and Tseday exhibited high mean yield of 1.09, 1.10, 1.07 and 1.07 tons/ha, respectively. AMMI stability value (ASV) ranged from 0.17 (genotype Lakech) to 1.40 (Amarach);yield stability index (YSI) from 7 (Lakech) to 25 (Quncho);and superiority measure (Pi) from 0.015 (Boset) to 0.145 (Dega Tef). Rank correlation of yield with Pi (r = 0.97, p < 0.01), ASV with Wi and δi2 (r = 0.85, p < 0.01) and that between Wi and δi2 (r = 1.00, p < 0.01) was high. Rank correlation of YSI with yield (r = 0.57, p < 0.05), ASV (r = 0.75, p < 0.01), and Pi (r = 0.68, p < 0.01) and Wi and δi2 (r = 0.67, p < 0.01) was also positive. The present study showed that genotypes Etsub, Simada and Tseday would be recommended for high yield and wide adaptation, and ASV would be used alone or jointly with YSI, Wi and δi2 for ranking of genotypes.

法语出国考试TEF/TCF分析指导

尽管目前法语出国考试TEF/TCF尚不被广大学生所熟悉,但它却是越来越多希望赴法留学学生必须参加的一项考试,因而他们迫切需要了解考试形式并期望得到相应指导。本文正是基于这一需求,试图对法语出国考试在中国的发展变化、目前国内主要的TEF与TCF两种考试形式及其应试策略、赴法留学的评估面试等问题进行详细阐述。

基于TEF模型的天津市旅游地生态安全评价

基于TEF(旅游生态足迹)模型,构建了旅游地生态安全评价指标体系。以天津市统计年鉴以及相关资料为依据,对天津市2011年旅游地生态安全进行评价,通过人均旅游生态足迹与人均旅游生态承载力的比值I来确定旅游地生态安全状况。结果表明,2011年天津市旅游地处于一般安全状态。在6种旅游要素中,交通旅游生态足迹所占比例最大,餐饮次之。针对天津市旅游地生态安全现状,提出了相应的旅游地生态安全维护措施。

绵羊不同繁殖状态下SIX1和TEF基因的表达特征分析

试验旨在探究SIX1和TEF基因在苏尼特羊(Sunite sheep,SNT)和小尾寒羊(Small Tail Han sheep,STH)相关组织中的表达特征,有助于揭示上述2个基因在绵羊季节性发情和繁殖调控中的重要作用。选取季节性发情的SNT母羊在短光照(模拟繁殖季节)和长光照(模拟休情期)条件下及常年发情的STH母羊在不同繁殖时期(卵泡期和黄体期)的下丘脑等10种组织,利用qPCR技术分析上述不同繁殖状态下各组织中SIX1和TEF基因的相对表达量。结果表明,SIX1基因在SNT和STH的垂体组织中均高表达,其它组织中微弱表达;TEF基因在2个绵羊品种的多个组织中广泛表达;SNT垂体中TEF基因在短光照条件下其表达量显著高于长光照条件(P<0.05),STH垂体中TEF基因在卵泡期其表达量显著高于黄体期(P<0.05);SNT子宫体中TEF在长光照条件下其表达量显著高于短光照条件(P<0.05),STH子宫体中TEF在黄体期其表达量极显著高于卵泡期(P<0.01)。研究结果显示,SIX1和TEF基因表达在绵羊垂体中发挥重要作用,2个基因在SNT垂体中的表达变化趋势与已知的长光照诱导基因EYA3的表达变化不同,暗示绵羊垂体中SIX1和TEF基因不是通过转录水平变化来参与季节性发情上游基因的调控;TEF基因可能参与绵羊不同繁殖状态下子宫生理变化的调控。本研究为深入探究这2个基因在绵羊繁殖性能调控方面的作用奠定了基础。

英国教学卓越框架(TEF)计划及其对我国的启示

英国高等教育在全球范围内享有很高的国际地位和声誉,为了进一步扩大英国高等教育的优势,保持英国大学在国际上的领导地位和影响力,英国政府决定将于2017年正式推行"教学卓越框架"(TEF)计划。这一计划第一次把高等教育教学基金与质量联系起来,对教学质量评估标准进行了调整,将关注的重点放在提高教学评估标准,保护弱势群体学生利益,以及重视学生未来职业发展方面,反映出教学评估过程中以质量为核心,以学生为本,多元主体参与和分阶段实施的特点。TEF计划的内容和特点,对提高我国高等教育教学质量,建设一流大学的目标有着借鉴和启示意义,值得进一步关注。

法语TEF考试与应试指导

要留学法国必须通过TEF考试(法语水平测试),TEF法语水平考试是一种新型权威的水平考试,越来越多希望赴法留学的学生迫切需要了解考试形式并期望得到相应指导。本文正是基于这一需求,试图对目前国内的TEF考试形式及其应试策略等问题进行详细阐述。

Tef rs738499基因多态性与帕金森病不同亚型合并抑郁的相关性

目的:探讨中国北方汉族人群Tefrs738499基因多态性与帕金森病不同亚型合并抑郁的相关性。方法:采用病例对照研究方法,选取230例帕金森病患者(PD组)和250例同期健康查体者(对照组),按运动症状及UPDRS评分将PD组分为震颤型、姿势障碍型、不确定型3种亚型,并根据HAMD评分将PD患者分为抑郁组(PDD组,n = 116)和非抑郁组(non-PDD, n = 114)。应用聚合酶链反应-限制性内切酶多态性(PCR-RFLP)方法检测Tef rs738499等位基因和基因型分布,并进行测序验证。结果:(1) 与对照组相比,该位点T等位基因在PD组中差别无统计学意义(OR: 0.983 95%CI: 0.720~1.342 P = 0.913),野生型基因型即TT基因型在PD组中差别无统计学意义(OR: 0.931 95%CI: 0.646~1.340 P = 0.699);(2) 将PD患者分为PDD与n-PDD组后,T等位基因在两组之间比较差别有统计学意义,T等位基因发生PDD的风险较n-PDD组是G等位基因的3.0倍(OR: 2.975 95%CI: 1.838~4.815 P <0.01),野生型基因型在两组之间比较差别有统计学意义,TT基因型发生PDD的风险是TG + GG型的4.6倍(OR: 4.646 95%CI: 2.637~8.186 P <0.01);(3) 该位点TT基因型在三种亚型分析中PDD组与n-PDD组均存在显著差异;(4) 病程、UPDRS-III评分、Tef rs738499的TT基因型是PDD的独立危险因素。结论:中国北方汉族人中,Tefrs738499 TT基因型及T等位基因与帕金森病不同亚型合并抑郁相关。

Effect of Phosphorus Rates and Varieties on Grain Yield, Nutrient Uptake and Phosphorus Efficiency of Tef [<i>Eragrostis tef</i>(Zucc.) Trotter]

Tef [Eragrostis tef (Zucc.) Trotter] is the major cereal crop in Ethiopia. Increasing tef yield requires improving soil phosphorus (P) supply and identifying P efficient varieties. An experiment was conducted at Wenago, Ethiopia, from May to August, 2011, during the main cropping season, to investigate the role of P supply in relation to grain yield, nutrient uptake (N, P, Ca and K) and P efficiency, and to investigate varietal differences for these parameters using four P rates (0, 3, 6 and 9 g/m2 P2O5) as main plots and three tef varieties (DZ-Cr-37, DZ-Cr-82, and DZ-Cr-255) as subplots in split-plot design with three replications. For respective 0, 3, 6 and 9 g/m2 P2O5, grain yield was 84, 203, 215 and 218 g/m2, total biomass 586, 897, 971 and 1016 g/m2, and harvest index 0.14, 0.23, 0.22 and 0.22. For respective variety DZ-Cr-37, DZ-Cr-82, and DZ-Cr-255, grain yield was 194, 182 and 163 g/m2, total biomass 810, 922 and 871 g/m2, and harvest index 0.24, 0.19 and 0.18. Total plant nutrients (g/m2) for respective 0, 3, 6 and 9 g/m2 P2O5 were N 3.92, 7.95, 9.49 and 10.80, P 0.57, 1.20, 1.49 and 1.66, calcium 0.16, 0.27, 0.38 and 0.45, and K 4.45, 7.96, 9.70 and 10.50. For respective 3, 6 and 9 g/m2 P2O5, P physiological efficiency (PE) was 224, 153 and 127, apparent recovery (AR) 0.49, 0.36 and 0.28, and agronomic efficiency (AE) 92, 50 and 35. For respective variety DZ-Cr-37, DZ-Cr-82 and DZ-Cr-255, PE was 248, 130 and 126, AR 0.28, 0.44 and 0.41, and AE 68, 57 and 51. The present experiment suggests that excess P supply beyond 3 g/m2 could result in low grain yield increase and low P recovery requiring soil P assessment prior to fertilizer application. Moreover, variety DZ-Cr-37 may be incorporated in the future breeding programs for P efficiency in tef.

Genetic Variation for Grain Yield and Yield Related Traits in Tef [<i>Eragrostis tef</i>(Zucc.)Trotter] under Moisture Stress and Non-Stress Environments

Tef [Eragrostis tef (Zucc.)Trotter] is an ancient and major cereal crop in Ethiopia. Increasing tef grain yield partly requires developing cultivars that are adapted to drought stress environment. An experiment was carried out using 18 tef genotypes grown during September to December, 2010, under two water supply environments (stress during grain filling period, and non-stress) to identify genetic variation, heritability and correlations of grain yield and yield related traits. Broad-sense heritability values under respective stress and non-stress environments were grain yield (g/m2) 0.80 and 0.89, total biomass (g/m2) 0.89 and 0.73, harvest index 0.69 and 0.79, panicle weight (g/plant) 0.93 and 0.92, and seed weight (g/plant) 0.96 and 0.86. The correlations of grain yield under respective stress and non-stress environments were total biomass rp = 0.64, rg = 0.70, and rp = 0.48, rg = 0.56, harvest index rp = 0.70, rg = 0.64, and rp = 0.87, rg = 0.90, panicle weight rp = 0.98, rg = 1.00, and rp = 0.96, rg = 1.00, and seed weight/plant rp = 0.98, rg = 1.00, and rp = 0.90, rg = 1.00. The present experiment showed that either grain yield per se, or seed weight/plant could be used to improve grain yield under stress and non-stress environments.

Effects of Soil Moisture and Fertilization Treatments on the Agronomic Traits of Teff (<i>Eragrosis tef</i>) in Southern Kyushu, Japan

This study investigated the agronomic characteristics of pot-cultured teff (Eragrostis tef) under soil moisture and fertilization treatments in southern Kyushu, Japan. In experiments examining the effects of soil moisture and fertilization, growth of teff and Setaria (Setaria sphacelata) as a reference species was compared under six treatments combining two soil moisture regimes, control (C) and high moisture (H) treatment, and three fertilizer applications, chemical fertilizer with manure (CF + M), chemical fertilizer (CF) and doubled level of manure (DM). H treatment did not decrease dry matter yield in teff two months after transplanting, except when combined with CF + M, compared with a decreasing tendency in Setaria. Top and underground dry matter yields and harvest index in teff increased significantly (P -2);6.2 gN m-2 is the standard level in the USA. Dry matter yield in teff did not differ significantly (P > 0.05) at CF application levels above 6.2 gN m-2 two months after sowing, suggesting that the optimum CF level of teff in southern Kyushu was comparable with that in the USA, and that teff had potential as an early-harvest forage crop two months after planting to replace summer forage in paddy fields of this region.

Response of Tef (<i>Eragrostis tef</i>(Zucc.) Trotter) to Balanced Fertilizer in Wolaita Zone, Southern Ethiopia

Tef is grown as an important domestic cereal in Ethiopia. Currently, global attention is given for it particularly as a “health food” due to the absence of gluten and gluten like proteins in its grains. Regardless of its wider adaptation, productivity of tef is low in the country with the national average grain yield of 1.379 tons·ha-1. This is mainly because of low soil fertility and severe organic matter depletion intensified by low rate of chemical fertilizer application. This study was conducted to evaluate effects of balanced fertilizers on the yield, yield components of tef and to determine economic feasibility for tef production in Wolaita. The experiment was laid out in a randomized complete block design (RCBD) with sixteen treatments replicated three times. The treatments consist of factorial combinations of four rates of K (Potassium) (0, 25, 50, and 100 kg/ha) and four rates of NPSB (Nitrogen, Phosphorus, Sulfur and Boron) (0, 50, 100, 200 kg/ha) fertilizers. Fertilizer types such as urea (46-0-0), NPSB (18.9-37.7-6.95-0.1) and K (0-0-60) were used as a source of nutrients. The soil analysis result indicated that, most of the nutrients are below optimum level to support the potential crop production. This may be related with reduced farm management practices and continuous cropping with little or no fertilizers input. In this study, it was found that, the combined application of NPSB and K fertilizers had a significant effect on growth, yield and yield components of tef. Among the treatments studied, NPSB (100 Kg/ha) and K (50 Kg/ha) gave greater grain yield. Furthermore, this treatment enhanced growth and yield related parameters compared to the control treatment. Thus, it is conceivable to recommend each to attain greater grain yield of tef in the study area. However, it is desirable to undertake further research across soil type, years and locations to appeal comprehensive recommendation on a wider scale.

英国TEF背景下的开放教育质量标准变革

英国近年来实施的TEF(教学卓越框架)计划,是20世纪90年代以QAA(高等教育质量保障局)建立健全高等教育学历标准、持续改进质量管理之后的一次重大改革。与QAA运行规则相比,TEF的根本变化,一是评估对象聚焦于本科教学;二是评估标准从基本质量到卓越要求;三是评估方式综合与多元化。TEF促使英国高校更加重视本科优质教育,不过,相比较而言,TEF计划对英国开放大学影响更大,其学生主要是本科生,规模全英第一,学生差异性大。TEF评估标准的新变化,有利于促进开放教育以学生为中心、追求卓越的教学质量、个性化的评估方式。