OBJECTIVE: To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction(藤龙补中汤,TBD), i.e Tenglong Buzhong granules(藤龙补中颗粒, TBG), in human SW620 colon cancer. METHODS: BALB/c nu...OBJECTIVE: To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction(藤龙补中汤,TBD), i.e Tenglong Buzhong granules(藤龙补中颗粒, TBG), in human SW620 colon cancer. METHODS: BALB/c nude mice were subcutaneously transplanted with SW620 cells, and treated with TBG(2.56 g/kg, once per day) and/or 5-Fu(104 mg/kg, once per week) for 21 d. Apoptosis, Caspase activities and cellular senescence were measured by commercial kits. The protein expression and phosphorylation were detected by Western blot or immunohistochemistry. RESULTS: TBG and 5-Fu inhibited tumor growth. The tumor inhibition rate of the TBG, 5-Fu, and TBG+5-Fu groups was 42.25%, 51.58%, and 76.08%, respectively. Combination of TBG and 5-Fu showed synergetic anticancer effects. TBG and 5-Fu induced apoptosis, activated caspase-3,-8, and-9, increased SMAC expression, inhibited XIAP expression. TBG induced cellular senescence, upregulated cyclin-dependent kinase inhibitor 1a(CDKN1a) and cyclin-dependent kinase inhibitor 2a(CDKN2a) expression, and inhibited phosphorylation of retinoblastoma-associated protein(RB) and expression of cyclin E1(CCNE1) and cyclindependent kinases(CDK) 2. TBG also inhibited angiogenesis accompanied by downregulation of vascular endothelial growth factor(VEGF) and hypoxiainducible factor-1α(HIF-1α). CONCLUSIONS: TBG inhibits SW620 colon cancer growth, induces apoptosis via SMAC-XIAP-Caspases signaling, induces cellular senescence through CDKN1a/CDKN2a-RB-E2F signaling, inhibits angiogenesis by down-regulation of HIF-1α and VEGF, and enhances the effects of 5-Fu.展开更多
目的大肠癌是全球常见恶性肿瘤;调节性T细胞(Regulatory T cells,Tregs)是一群免疫负调控细胞,参与大肠癌的发病,与大肠癌预后直接相关。藤龙补中汤是以解毒健脾立法的中药复方,细胞与动物模型研究显示藤龙补中汤可以促大肠癌细胞凋亡...目的大肠癌是全球常见恶性肿瘤;调节性T细胞(Regulatory T cells,Tregs)是一群免疫负调控细胞,参与大肠癌的发病,与大肠癌预后直接相关。藤龙补中汤是以解毒健脾立法的中药复方,细胞与动物模型研究显示藤龙补中汤可以促大肠癌细胞凋亡、细胞衰老,增强化疗对大肠癌的疗效。本研究观察了藤龙补中汤对晚期大肠癌患者Tregs的作用。方法晚期大肠癌患者随机分为对照组及治疗组,分别给化疗或藤龙补中汤联合化疗治疗;观察患者治疗前后Tregs细胞及相关细胞因子变化。流式细胞仪检测Tregs细胞,酶联免疫吸附实验(Enzyme-Linked Immunosorbent Assay,ELISA)检测细胞因子。结果治疗组治疗后调节性T细胞及白介素-10(Interleukin-10,IL-10)、转化生长因子-β(Transforming growth factor-β,TGF-β)水平显著降低(P<0.01),与对照组比较差异显著(P<0.01)。结论藤龙补中汤可以降低晚期大肠癌患者Tregs细胞数量,以相关细胞因子IL-10、TGF-β水平。展开更多
基金Supported by Natural Science Foundation of Shanghai Municipality:Tenglong Buzhong Decoction Inhibiting Metastasis of Colorectal Cancer by Regulation of HIF-1 α/LOX/PTK2 Signal Transduction (No. 20ZR1458700)Program from Science and Technology Commission of Shanghai Municipality:Real World Study of Tenglong Buzhong Decoction on Colorectal Cancer Metastasis and Circulating miRNAs (No. 19401933400)。
文摘OBJECTIVE: To observe the anticancer effects of the granular preparation of Tenglong Buzhong decoction(藤龙补中汤,TBD), i.e Tenglong Buzhong granules(藤龙补中颗粒, TBG), in human SW620 colon cancer. METHODS: BALB/c nude mice were subcutaneously transplanted with SW620 cells, and treated with TBG(2.56 g/kg, once per day) and/or 5-Fu(104 mg/kg, once per week) for 21 d. Apoptosis, Caspase activities and cellular senescence were measured by commercial kits. The protein expression and phosphorylation were detected by Western blot or immunohistochemistry. RESULTS: TBG and 5-Fu inhibited tumor growth. The tumor inhibition rate of the TBG, 5-Fu, and TBG+5-Fu groups was 42.25%, 51.58%, and 76.08%, respectively. Combination of TBG and 5-Fu showed synergetic anticancer effects. TBG and 5-Fu induced apoptosis, activated caspase-3,-8, and-9, increased SMAC expression, inhibited XIAP expression. TBG induced cellular senescence, upregulated cyclin-dependent kinase inhibitor 1a(CDKN1a) and cyclin-dependent kinase inhibitor 2a(CDKN2a) expression, and inhibited phosphorylation of retinoblastoma-associated protein(RB) and expression of cyclin E1(CCNE1) and cyclindependent kinases(CDK) 2. TBG also inhibited angiogenesis accompanied by downregulation of vascular endothelial growth factor(VEGF) and hypoxiainducible factor-1α(HIF-1α). CONCLUSIONS: TBG inhibits SW620 colon cancer growth, induces apoptosis via SMAC-XIAP-Caspases signaling, induces cellular senescence through CDKN1a/CDKN2a-RB-E2F signaling, inhibits angiogenesis by down-regulation of HIF-1α and VEGF, and enhances the effects of 5-Fu.
