OBJECTIVE Tenuifolin is a natu⁃ral neuroprotective compound isolated from Polygala tenuifolia Willd,which have a long history of being used in mental illness therapy in China.Acute or chronic administration of NMDA re...OBJECTIVE Tenuifolin is a natu⁃ral neuroprotective compound isolated from Polygala tenuifolia Willd,which have a long history of being used in mental illness therapy in China.Acute or chronic administration of NMDA recep⁃tor blocker MK-801 is a validated animal model for the positive,negative and cognitive symp⁃toms of schizophrenia.This study aimed to inves⁃tigate the antipsychotic effects and possible mechanism of tenuifolin in MK-801-treated mice.METHODS Sensorimotor gating deficit and hyper⁃locomotion,which implying positive symptoms,were assessed by prepulse inhibition(PPI)test and open field test.Social withdrawal,a main character of negative symptoms,was assessed by social interaction tests.Cognitive deficit was assessed by novel object recognition(NOR)test.The level of glutamate decarboxylase(GAD),GABA and monoamines were detected by West⁃ern blotting or HPLC-ECD.RESULTS Acute administration of MK-801(0.1 mg·kg^(-1),sc)could significantly induce hyperlocomotion and prepulse inhibition(PPI)deficit in mice,which could be reversed by the treatment of tenuifolin(40 and 80 mg·kg^(-1),ig).Moreover,tenuifolin ameliorated social withdrawal and cognitive deficit induced by the chronic administration of MK-801(0.3 mg·kg^(-1) per day,sc,14 d followed by 7 d washout period).In addition,tenuifolin reversed the reduction in GAD and GABA induced by MK-801 and normal⁃ized dopamine,serotonin and norepinephrine in the prefrontal cortex.CONCLUSION These results provided persuasive evidence that tenui⁃folin has multiple antipsychotic-like effects in ani⁃mal model of schizophrenia and its underlying mechanism may be associated with its potential effects on GABA function in the prefrontal cortex.展开更多
目的：建立镇癎康脑A胶囊的成分研究方法。方法：采用显微鉴别法对镇癎康脑A胶囊中的全蝎、蜈蚣进行鉴别;采用薄层色谱（TLC）法对柴胡、天麻进行鉴别;采用HPLC法测定制剂中细叶远志皂苷的含量,色谱条件：Phenomenex Luna 5u18（2）100...目的：建立镇癎康脑A胶囊的成分研究方法。方法：采用显微鉴别法对镇癎康脑A胶囊中的全蝎、蜈蚣进行鉴别;采用薄层色谱（TLC）法对柴胡、天麻进行鉴别;采用HPLC法测定制剂中细叶远志皂苷的含量,色谱条件：Phenomenex Luna 5u18（2）100AC_（18）柱（250mm×4.6mm,5μm）;流动相：甲醇-0.05%磷酸水溶液（69∶31）;检测波长：210nm;流速：1.0ml/min;进样量为20μl。结果：显微镜下,镇康脑A胶囊中的全蝎可见棕褐色刚毛,蜈蚣有明显的气管碎片;柴胡、天麻TLC鉴别斑点清晰,分离效果好;细叶远志皂苷在80.2～802.5μg/ml范围内线性关系良好（r=0.999 3）,精密度、稳定性、重复性实验的RSD均〈2%,平均加样回收率为（101.0±1.02）%（n=6）。结论：该方法操作简单,灵敏度高,专属性强,重复性好,可用于镇康脑A胶囊的成分研究。展开更多
文摘OBJECTIVE Tenuifolin is a natu⁃ral neuroprotective compound isolated from Polygala tenuifolia Willd,which have a long history of being used in mental illness therapy in China.Acute or chronic administration of NMDA recep⁃tor blocker MK-801 is a validated animal model for the positive,negative and cognitive symp⁃toms of schizophrenia.This study aimed to inves⁃tigate the antipsychotic effects and possible mechanism of tenuifolin in MK-801-treated mice.METHODS Sensorimotor gating deficit and hyper⁃locomotion,which implying positive symptoms,were assessed by prepulse inhibition(PPI)test and open field test.Social withdrawal,a main character of negative symptoms,was assessed by social interaction tests.Cognitive deficit was assessed by novel object recognition(NOR)test.The level of glutamate decarboxylase(GAD),GABA and monoamines were detected by West⁃ern blotting or HPLC-ECD.RESULTS Acute administration of MK-801(0.1 mg·kg^(-1),sc)could significantly induce hyperlocomotion and prepulse inhibition(PPI)deficit in mice,which could be reversed by the treatment of tenuifolin(40 and 80 mg·kg^(-1),ig).Moreover,tenuifolin ameliorated social withdrawal and cognitive deficit induced by the chronic administration of MK-801(0.3 mg·kg^(-1) per day,sc,14 d followed by 7 d washout period).In addition,tenuifolin reversed the reduction in GAD and GABA induced by MK-801 and normal⁃ized dopamine,serotonin and norepinephrine in the prefrontal cortex.CONCLUSION These results provided persuasive evidence that tenui⁃folin has multiple antipsychotic-like effects in ani⁃mal model of schizophrenia and its underlying mechanism may be associated with its potential effects on GABA function in the prefrontal cortex.
文摘目的：建立镇癎康脑A胶囊的成分研究方法。方法：采用显微鉴别法对镇癎康脑A胶囊中的全蝎、蜈蚣进行鉴别;采用薄层色谱（TLC）法对柴胡、天麻进行鉴别;采用HPLC法测定制剂中细叶远志皂苷的含量,色谱条件：Phenomenex Luna 5u18（2）100AC_（18）柱（250mm×4.6mm,5μm）;流动相：甲醇-0.05%磷酸水溶液（69∶31）;检测波长：210nm;流速：1.0ml/min;进样量为20μl。结果：显微镜下,镇康脑A胶囊中的全蝎可见棕褐色刚毛,蜈蚣有明显的气管碎片;柴胡、天麻TLC鉴别斑点清晰,分离效果好;细叶远志皂苷在80.2～802.5μg/ml范围内线性关系良好（r=0.999 3）,精密度、稳定性、重复性实验的RSD均〈2%,平均加样回收率为（101.0±1.02）%（n=6）。结论：该方法操作简单,灵敏度高,专属性强,重复性好,可用于镇康脑A胶囊的成分研究。
