Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard D...Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard Diagnostics) Bio line, South Korea was evaluated. The ICT is a rapid, reliable and cheaper method that can be used instead of conventional biochemical tests for confirming MTB in culture isolates in resource limited laboratories. The study also evaluated the ability of ICT to detect MPT64-Antigen before the micro MGIT could signal positive. Material/Methods: A total of 450 sputum samples of individual patients were used for the study. 152 isolates of Mycobacteria were recovered from solid and liquid media. These strains were tested for the detection of MPT64-antigen. H37Rv strain was served as the positive reference control and also used for early detection of Antigen experiment. Findings: The development of bands on both test and sample region when H37Rv strain was tested were seen (MPT64 antigen positive). When 138 MTB isolates were tested, it showed a similar banding pattern indicating 100% sensitivity. MPT64 band formation was not detected in any of the 14 isolates indicating 100% specificity. Both PPV & NPV were 100%. All the isolates negative for MPT64 Ag were confirmed as MOTT by conventional bio-chemical PNBA. The H37Rv strain showed a faint band from the 2nd day onwards from inoculation till 3rd day in the earlier Antigen detection experiment. Conclusion: Rapid identification of MTB culture isolate is a pressing need for diagnosis and proceeding to perform drug susceptibility testing. MPT64 TB Ag detection ICT kit is a rapid, reliable method, good substitute for molecular identification methods, and conventional biochemical test which is time-consuming and technically demanding. The early detection of Antigen can be used as an effective tool in diagnosis.展开更多
A newly designed shock-tube for biological testing has been built in our labo-ratory.This tube is 39.34 m long.It consists of several sections:high pressure section,divergent section,transitional section,test section ...A newly designed shock-tube for biological testing has been built in our labo-ratory.This tube is 39.34 m long.It consists of several sections:high pressure section,divergent section,transitional section,test section and wave-dissipated section.In theopen condition,the maximal overpressure is about 214,3 kPa,while in the closed condi-tion,the maximal overpressure may go up to 630.3 kPa.The energy source is compres-sed air.Using this equipment,we are able to inflict blast injuries with various degreesof severity in rabbits,dogs and sheep.展开更多
Performance characterization for massive photomultiplier tubes(PMTs) is a frequently encountered procedure in large nuclear and particle experiments.To facilitate this work,a dedicated test bench system has been devel...Performance characterization for massive photomultiplier tubes(PMTs) is a frequently encountered procedure in large nuclear and particle experiments.To facilitate this work,a dedicated test bench system has been developed at the Institute of Modern Physics,Chinese Academy of Sciences.The two-dimensional photocathode position scanning capability is an intrinsic function of the test bench,and up to 25 PMTs,with the diameter smaller than 2",can be tested simultaneously.The parameters of the light source pulses can be adjusted in a wide range,thus making it suitable for various characteristics measurements.The test bench system is highly automated with all the controlling operations integrated into a single software.Additionally,the hardware platform is extensible which allows complex testing schemes,and the modularity in the software design makes the migration from one testing configuration to another light-weight and efficient.All these features make the test bench versatile and reusable in different experiments.It has been first used in the construction of the Plastic Scintillator Detector(PSD) of DArk Matter Particle Explorer(DAMPE),and a total of 570 Hamamatsu R4443 tubes were tested successfully.The performance was verified and the testing results are also reported in this article.展开更多
In this paper, the Ground Penetrating Radar (GPR) method was used to characterize concrete tubes and steel/plastic tanks buried in IAG/USP test site. The microwave tomography was used to improve the GPR images, aiming...In this paper, the Ground Penetrating Radar (GPR) method was used to characterize concrete tubes and steel/plastic tanks buried in IAG/USP test site. The microwave tomography was used to improve the GPR images, aiming to retrieve the geometry of the targets. The numerical modeling studies also were done in order to predict the GPR results of the buried targets and to give more reliability to the results interpretation. The targets were installed in the first shallow geophysical test site of the Brazil located at Institute of Astronomy, Geophysics, and Atmospheric Science (IAG) of the University of S?o Paulo (USP). GPR profiles of 200 MHz (shielded bistatic antennas) were acquired along three lines containing concrete tubes and steel/plastic tanks buried in subsoil. The concrete tubes show a hyperbolic reflector for the top, and the vertical tube also presented a reflection on its bottom. The horizontal steel tanks were characterized by a strong GPR reflection on their top. The empty plastic tank shows a strong reflector for the top with normal polarity. On the other hand, the plastic tank filled with water shows a weaker reflector for its top characterized by the inverted polarity of GPR signal when compared with empty plastic tank. The plastic tank filled with water also went characterized by the strong reflection to its bottom, being a good indicative to interpret GPR data on target in subsoil with some types of fluid inside of tank. The results of polarity difference for the top of tank can be used as guide pattern to identify buried tank empty or filled with water. The application of microwave tomography to the GPR data permitted to determine the position and get a good identification of the edges of the targets studied. The numeric modeling presented a good accordance with real data reducing the ambiguities in interpretation of results. These results can be used as a reference, and they can be extrapolated for areas where there is no subsurface information.展开更多
"Generalized mobility"is used to realize the unification of tube flow and seepage in form and the unification of commonly used linear and nonlinear flow laws in form,which makes it possible to use the same f..."Generalized mobility"is used to realize the unification of tube flow and seepage in form and the unification of commonly used linear and nonlinear flow laws in form,which makes it possible to use the same form of motion equations to construct unified governing equations for reservoirs of different scales in different regions.Firstly,by defining the generalized mobility under different flow conditions,the basic equation governing fluid flow in reservoir coupling generalized tube flow and seepage is established.Secondly,two typical well test analysis models for coupling tube flow and seepage flow are given,namely,pipe-shaped composite reservoir model and partially open cylindrical reservoir model.The log-log pressure draw-down type-curve of composite pipe-shaped reservoir model can show characteristics of two sets of linear flow.The log-log pressure drawdown plot of partially opened cylindrical reservoir model can show the characteristics of spherical flow and linear flow,as well as spherical flow and radial flow.The pressure build-up derivative curves of the two models basically coincide with their respective pressure drawdown derivative curves in the early stage,pulling down features in the late stage,and the shorter the production time is,the earlier the pulling down feature appears.Finally,the practicability and reliability of the models presented in this paper are verified by three application examples.展开更多
A design for an air-cooled test tube, with a series of modular and movable rings (cylindrical “crystallization comb”), installed in a laboratory crucible furnace is presented. The setup allows easy regulation simult...A design for an air-cooled test tube, with a series of modular and movable rings (cylindrical “crystallization comb”), installed in a laboratory crucible furnace is presented. The setup allows easy regulation simultaneous crystallization tests of a series of different crystallization rates in several columns (matrix) of test tubes, enabling fast studies of obtaining crystals. This low-budget, portable device (i.e. adjustable airstream with more simple control options), can also be applied in tube and chamber furnaces. The relations between the crystallization rate and parameters of air-cooled test tube are given and numerically analyzed.展开更多
文摘Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard Diagnostics) Bio line, South Korea was evaluated. The ICT is a rapid, reliable and cheaper method that can be used instead of conventional biochemical tests for confirming MTB in culture isolates in resource limited laboratories. The study also evaluated the ability of ICT to detect MPT64-Antigen before the micro MGIT could signal positive. Material/Methods: A total of 450 sputum samples of individual patients were used for the study. 152 isolates of Mycobacteria were recovered from solid and liquid media. These strains were tested for the detection of MPT64-antigen. H37Rv strain was served as the positive reference control and also used for early detection of Antigen experiment. Findings: The development of bands on both test and sample region when H37Rv strain was tested were seen (MPT64 antigen positive). When 138 MTB isolates were tested, it showed a similar banding pattern indicating 100% sensitivity. MPT64 band formation was not detected in any of the 14 isolates indicating 100% specificity. Both PPV & NPV were 100%. All the isolates negative for MPT64 Ag were confirmed as MOTT by conventional bio-chemical PNBA. The H37Rv strain showed a faint band from the 2nd day onwards from inoculation till 3rd day in the earlier Antigen detection experiment. Conclusion: Rapid identification of MTB culture isolate is a pressing need for diagnosis and proceeding to perform drug susceptibility testing. MPT64 TB Ag detection ICT kit is a rapid, reliable method, good substitute for molecular identification methods, and conventional biochemical test which is time-consuming and technically demanding. The early detection of Antigen can be used as an effective tool in diagnosis.
文摘A newly designed shock-tube for biological testing has been built in our labo-ratory.This tube is 39.34 m long.It consists of several sections:high pressure section,divergent section,transitional section,test section and wave-dissipated section.In theopen condition,the maximal overpressure is about 214,3 kPa,while in the closed condi-tion,the maximal overpressure may go up to 630.3 kPa.The energy source is compres-sed air.Using this equipment,we are able to inflict blast injuries with various degreesof severity in rabbits,dogs and sheep.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Science(No.XDA04040202-3)
文摘Performance characterization for massive photomultiplier tubes(PMTs) is a frequently encountered procedure in large nuclear and particle experiments.To facilitate this work,a dedicated test bench system has been developed at the Institute of Modern Physics,Chinese Academy of Sciences.The two-dimensional photocathode position scanning capability is an intrinsic function of the test bench,and up to 25 PMTs,with the diameter smaller than 2",can be tested simultaneously.The parameters of the light source pulses can be adjusted in a wide range,thus making it suitable for various characteristics measurements.The test bench system is highly automated with all the controlling operations integrated into a single software.Additionally,the hardware platform is extensible which allows complex testing schemes,and the modularity in the software design makes the migration from one testing configuration to another light-weight and efficient.All these features make the test bench versatile and reusable in different experiments.It has been first used in the construction of the Plastic Scintillator Detector(PSD) of DArk Matter Particle Explorer(DAMPE),and a total of 570 Hamamatsu R4443 tubes were tested successfully.The performance was verified and the testing results are also reported in this article.
文摘In this paper, the Ground Penetrating Radar (GPR) method was used to characterize concrete tubes and steel/plastic tanks buried in IAG/USP test site. The microwave tomography was used to improve the GPR images, aiming to retrieve the geometry of the targets. The numerical modeling studies also were done in order to predict the GPR results of the buried targets and to give more reliability to the results interpretation. The targets were installed in the first shallow geophysical test site of the Brazil located at Institute of Astronomy, Geophysics, and Atmospheric Science (IAG) of the University of S?o Paulo (USP). GPR profiles of 200 MHz (shielded bistatic antennas) were acquired along three lines containing concrete tubes and steel/plastic tanks buried in subsoil. The concrete tubes show a hyperbolic reflector for the top, and the vertical tube also presented a reflection on its bottom. The horizontal steel tanks were characterized by a strong GPR reflection on their top. The empty plastic tank shows a strong reflector for the top with normal polarity. On the other hand, the plastic tank filled with water shows a weaker reflector for its top characterized by the inverted polarity of GPR signal when compared with empty plastic tank. The plastic tank filled with water also went characterized by the strong reflection to its bottom, being a good indicative to interpret GPR data on target in subsoil with some types of fluid inside of tank. The results of polarity difference for the top of tank can be used as guide pattern to identify buried tank empty or filled with water. The application of microwave tomography to the GPR data permitted to determine the position and get a good identification of the edges of the targets studied. The numeric modeling presented a good accordance with real data reducing the ambiguities in interpretation of results. These results can be used as a reference, and they can be extrapolated for areas where there is no subsurface information.
基金Supported by the Scientific Research Project of Key Laboratory of Shaanxi Provincial Department of Education(13JS090)。
文摘"Generalized mobility"is used to realize the unification of tube flow and seepage in form and the unification of commonly used linear and nonlinear flow laws in form,which makes it possible to use the same form of motion equations to construct unified governing equations for reservoirs of different scales in different regions.Firstly,by defining the generalized mobility under different flow conditions,the basic equation governing fluid flow in reservoir coupling generalized tube flow and seepage is established.Secondly,two typical well test analysis models for coupling tube flow and seepage flow are given,namely,pipe-shaped composite reservoir model and partially open cylindrical reservoir model.The log-log pressure draw-down type-curve of composite pipe-shaped reservoir model can show characteristics of two sets of linear flow.The log-log pressure drawdown plot of partially opened cylindrical reservoir model can show the characteristics of spherical flow and linear flow,as well as spherical flow and radial flow.The pressure build-up derivative curves of the two models basically coincide with their respective pressure drawdown derivative curves in the early stage,pulling down features in the late stage,and the shorter the production time is,the earlier the pulling down feature appears.Finally,the practicability and reliability of the models presented in this paper are verified by three application examples.
文摘A design for an air-cooled test tube, with a series of modular and movable rings (cylindrical “crystallization comb”), installed in a laboratory crucible furnace is presented. The setup allows easy regulation simultaneous crystallization tests of a series of different crystallization rates in several columns (matrix) of test tubes, enabling fast studies of obtaining crystals. This low-budget, portable device (i.e. adjustable airstream with more simple control options), can also be applied in tube and chamber furnaces. The relations between the crystallization rate and parameters of air-cooled test tube are given and numerically analyzed.