Quercetin （QE） and resveratrol （RSV） are powerful antioxidants with the potential to protect the testes against ischemia/reperfusion （I/R） injury. We compared their effects in testicular torsion/detorsion （T/D...Quercetin （QE） and resveratrol （RSV） are powerful antioxidants with the potential to protect the testes against ischemia/reperfusion （I/R） injury. We compared their effects in testicular torsion/detorsion （T/D） in adult rats. Twenty-four male Wistar rats were divided into four groups： sham （group A）, T/D （group B）, T/D treated with QE （group C）, and T/D treated with RSV （group D）. QE （20 mg kg-1） and RSV （20 mg kg-1） were injected intra-peritoneally at 60 min of torsion. After 90 rain of surgically induced torsion, the testicular cord was restored to its anatomical position. Twenty-four hour after torsion, blood and tissue samples were obtained for further examination. Testicular tissue malondialdehyde （MDA） and nitric oxide （NO） levels and serum total oxidant status （TOS） were higher in group B than in group A （P 〈 0.05）. Group A had higher serum total antioxidant status （TAS） than group B. （P 〈 0.05） QE and RSV significantly lowered MDA, NO, and TOS levels and TAS consumption （P 〈 0.05）. QE reduced the MDA and TOS levels more than RSV （P〈 0.05）, but their effects on NO reduction and TAS consumption were similar （P〉 0.05）. Group A had normal testicular architecture （grade 1）. Groups C （mean grade 2.60） and D （mean grade 3.00） had lower testicular injury grades than group B （mean grade 3.45） （P 〈 0.05）. Group C had lower testicular injury grade than group D （P 〈 0.05）. Treatment with QE and RSV protects against I/R injury after testicular T/D. QE may exhibit better function than RSV at the doses tested in this study.展开更多
Objective To investigate the apoptosis of epididyrnis epithelium and the change of epididymissialic acid following torsed/detorsed testes. Methods Twenty four adult male Sprague-Dawley rats were subjected to unilatera...Objective To investigate the apoptosis of epididyrnis epithelium and the change of epididymissialic acid following torsed/detorsed testes. Methods Twenty four adult male Sprague-Dawley rats were subjected to unilateral 720 testicular torsion with the duration of 2h and 4h, then repaired. The ischemic epididymis were collected for detecting the content of sialic acid by using spectrophotometry and the apoptosis with TUNEL technique. Results There were no statistically significant difference in the apoptosis of epididymis epithelium [(9.51± 2.78)% vs (6.34±1. 98)%] and the content of epididymis sialic acid(23, 3851 ± 9. 2199mg/mgprot vs 19. 3661 6. 3373mg/mgprot) at 24h between following 2h-torsed/detorsed testes and those of sham group. There were statistically significant difference in the apopotosis of epididymis epithelium[ (46. 81 ±3. 55)% vs (6. 34±1. 98) % ] and the content of epididymis sialic acid (13. 7249±7. 8006mg/mgprot vs 19. 3661±6. 3373mg/mgprot) at 24h between following 4h-torsed/detorsed testes and those of sham group(P <0. 05). Conclusion The results suggest that the sialic acid-secreting-function of epididymis remain normal at 24h following 2h-torsed/detorsed testes, while the apoptosis index of epididymis epithelium do not increase. The epididymis would be injured at 24h following 4h-torsed/detorsed testes, while the apoptosis index increased.展开更多
文摘Quercetin （QE） and resveratrol （RSV） are powerful antioxidants with the potential to protect the testes against ischemia/reperfusion （I/R） injury. We compared their effects in testicular torsion/detorsion （T/D） in adult rats. Twenty-four male Wistar rats were divided into four groups： sham （group A）, T/D （group B）, T/D treated with QE （group C）, and T/D treated with RSV （group D）. QE （20 mg kg-1） and RSV （20 mg kg-1） were injected intra-peritoneally at 60 min of torsion. After 90 rain of surgically induced torsion, the testicular cord was restored to its anatomical position. Twenty-four hour after torsion, blood and tissue samples were obtained for further examination. Testicular tissue malondialdehyde （MDA） and nitric oxide （NO） levels and serum total oxidant status （TOS） were higher in group B than in group A （P 〈 0.05）. Group A had higher serum total antioxidant status （TAS） than group B. （P 〈 0.05） QE and RSV significantly lowered MDA, NO, and TOS levels and TAS consumption （P 〈 0.05）. QE reduced the MDA and TOS levels more than RSV （P〈 0.05）, but their effects on NO reduction and TAS consumption were similar （P〉 0.05）. Group A had normal testicular architecture （grade 1）. Groups C （mean grade 2.60） and D （mean grade 3.00） had lower testicular injury grades than group B （mean grade 3.45） （P 〈 0.05）. Group C had lower testicular injury grade than group D （P 〈 0.05）. Treatment with QE and RSV protects against I/R injury after testicular T/D. QE may exhibit better function than RSV at the doses tested in this study.
文摘Objective To investigate the apoptosis of epididyrnis epithelium and the change of epididymissialic acid following torsed/detorsed testes. Methods Twenty four adult male Sprague-Dawley rats were subjected to unilateral 720 testicular torsion with the duration of 2h and 4h, then repaired. The ischemic epididymis were collected for detecting the content of sialic acid by using spectrophotometry and the apoptosis with TUNEL technique. Results There were no statistically significant difference in the apoptosis of epididymis epithelium [(9.51± 2.78)% vs (6.34±1. 98)%] and the content of epididymis sialic acid(23, 3851 ± 9. 2199mg/mgprot vs 19. 3661 6. 3373mg/mgprot) at 24h between following 2h-torsed/detorsed testes and those of sham group. There were statistically significant difference in the apopotosis of epididymis epithelium[ (46. 81 ±3. 55)% vs (6. 34±1. 98) % ] and the content of epididymis sialic acid (13. 7249±7. 8006mg/mgprot vs 19. 3661±6. 3373mg/mgprot) at 24h between following 4h-torsed/detorsed testes and those of sham group(P <0. 05). Conclusion The results suggest that the sialic acid-secreting-function of epididymis remain normal at 24h following 2h-torsed/detorsed testes, while the apoptosis index of epididymis epithelium do not increase. The epididymis would be injured at 24h following 4h-torsed/detorsed testes, while the apoptosis index increased.
