We evaluated the function of the fresh and cryopresereed newborn rat testicular tissue transplanted into outbred adult castrated male rats without immunosuppression for 30 days.Testis grafts were evaluated morphologic...We evaluated the function of the fresh and cryopresereed newborn rat testicular tissue transplanted into outbred adult castrated male rats without immunosuppression for 30 days.Testis grafts were evaluated morphologically and histologically,and testosterone(T)levels of serum and homogenate of testis grafts were determined in recipients by 30 days after transplantation.The fresh tissues(GroupI)and cryopreserved tissues(Group Ⅱ),from the 2nd or 3rd day of postnatal,showed an overall increase in growth,with maintenance of architecture and minimal lymphocytic infiltration.Thirty days after implantation,mean(±s)serum T concentrations(0.986±0.028 nmol/L in group I;1.113±0.201 nmol/L in group Ⅱ)were significantly higher than that prior to transplantation(<0.347 nmol/L,respectively;P<0.01 and P<0.01).After transplantation,however,mean serum T levels between two groups were not significantly different(P<0.05),and their grafting homogenate T levels(0.944±0.164 nmol/g in group I;1.019±0.172 nmol/g in group Ⅱ) were also not significantly different between two groups(P>0.05).Our data suggest that the testicular tissue of newborn rats can not only survive,grow and differentiate,but also can produce enough T detectable in vivo after transplantation and no significant differences were found between fresh tissue and frozen tissue transplanted.展开更多
背景:干细胞移植治疗雄性激素部分性缺乏综合征鲜有报道。目的:观察骨髓间充质干细胞移植对老年大鼠性激素水平及睾丸组织的影响。方法:取30只6月龄SD大鼠作为正常对照组,检测血清睾酮和游离睾酮浓度;选择血清睾酮和游离睾酮浓度低于正...背景:干细胞移植治疗雄性激素部分性缺乏综合征鲜有报道。目的:观察骨髓间充质干细胞移植对老年大鼠性激素水平及睾丸组织的影响。方法:取30只6月龄SD大鼠作为正常对照组,检测血清睾酮和游离睾酮浓度;选择血清睾酮和游离睾酮浓度低于正常对照组的90只20月龄SD大鼠,作为雄性激素部分性缺乏综合征模型,随机分为3组,模型组、原位移植组与尾静脉移植组,原位移植组在睾丸内注射2×10~8 L^(-1)的骨髓间充质干细胞悬液0.2 m L,尾静脉移植组经尾静脉注射2×10~8 L^(-1)的骨髓间充质干细胞悬液0.5 m L。移植8周后,检测各组血清中卵泡刺激素、促黄体激素及睾酮水平,并制作睾丸组织病理切片,观察睾丸组织及间质细胞情况。结果与结论:(1)血清检测结果:4组间卵泡刺激素、促黄体激素水平比较差异均无显著性意义;模型组睾酮水平明显低于正常对照组(P≤0.01),原位移植组、尾静脉移植组睾酮水平明显高于模型组(P≤0.01);(2)苏木精-伊红染色结果:正常对照组睾丸组织完整,可见大量间质细胞、支持细胞及各级生精细胞;模型组睾丸组织缺失,支持细胞与间质细胞稀疏,缺乏精原细胞与生精细胞;原位移植组、尾静脉移植组睾丸组织较完整,可见大量间质细胞与支持细胞或生精细胞;(3)NBT染色结果:模型组睾丸间质细胞数量明显少于正常对照组(P≤0.01),原位移植组、尾静脉移植组睾丸间质细胞数量明显多于模型组(P≤0.01);(4)结果表明:骨髓间充质干细胞移植对老年大鼠睾丸组织有修复作用。展开更多
文摘We evaluated the function of the fresh and cryopresereed newborn rat testicular tissue transplanted into outbred adult castrated male rats without immunosuppression for 30 days.Testis grafts were evaluated morphologically and histologically,and testosterone(T)levels of serum and homogenate of testis grafts were determined in recipients by 30 days after transplantation.The fresh tissues(GroupI)and cryopreserved tissues(Group Ⅱ),from the 2nd or 3rd day of postnatal,showed an overall increase in growth,with maintenance of architecture and minimal lymphocytic infiltration.Thirty days after implantation,mean(±s)serum T concentrations(0.986±0.028 nmol/L in group I;1.113±0.201 nmol/L in group Ⅱ)were significantly higher than that prior to transplantation(<0.347 nmol/L,respectively;P<0.01 and P<0.01).After transplantation,however,mean serum T levels between two groups were not significantly different(P<0.05),and their grafting homogenate T levels(0.944±0.164 nmol/g in group I;1.019±0.172 nmol/g in group Ⅱ) were also not significantly different between two groups(P>0.05).Our data suggest that the testicular tissue of newborn rats can not only survive,grow and differentiate,but also can produce enough T detectable in vivo after transplantation and no significant differences were found between fresh tissue and frozen tissue transplanted.
文摘背景:干细胞移植治疗雄性激素部分性缺乏综合征鲜有报道。目的:观察骨髓间充质干细胞移植对老年大鼠性激素水平及睾丸组织的影响。方法:取30只6月龄SD大鼠作为正常对照组,检测血清睾酮和游离睾酮浓度;选择血清睾酮和游离睾酮浓度低于正常对照组的90只20月龄SD大鼠,作为雄性激素部分性缺乏综合征模型,随机分为3组,模型组、原位移植组与尾静脉移植组,原位移植组在睾丸内注射2×10~8 L^(-1)的骨髓间充质干细胞悬液0.2 m L,尾静脉移植组经尾静脉注射2×10~8 L^(-1)的骨髓间充质干细胞悬液0.5 m L。移植8周后,检测各组血清中卵泡刺激素、促黄体激素及睾酮水平,并制作睾丸组织病理切片,观察睾丸组织及间质细胞情况。结果与结论:(1)血清检测结果:4组间卵泡刺激素、促黄体激素水平比较差异均无显著性意义;模型组睾酮水平明显低于正常对照组(P≤0.01),原位移植组、尾静脉移植组睾酮水平明显高于模型组(P≤0.01);(2)苏木精-伊红染色结果:正常对照组睾丸组织完整,可见大量间质细胞、支持细胞及各级生精细胞;模型组睾丸组织缺失,支持细胞与间质细胞稀疏,缺乏精原细胞与生精细胞;原位移植组、尾静脉移植组睾丸组织较完整,可见大量间质细胞与支持细胞或生精细胞;(3)NBT染色结果:模型组睾丸间质细胞数量明显少于正常对照组(P≤0.01),原位移植组、尾静脉移植组睾丸间质细胞数量明显多于模型组(P≤0.01);(4)结果表明:骨髓间充质干细胞移植对老年大鼠睾丸组织有修复作用。
